Online citations, reference lists, and bibliographies.

Neonatal Rat Primary Microglia: Isolation, Culturing, And Selected Applications

Mingwei Ni, M. Aschner
Published 2010 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Microglial cells elaborate trophic factors and cytokines and remove toxins and debris from the extracellular space in the central nervous system, acting analogously to peripheral macrophages. Over the past two decades, increased attention has been directed at the role of microglia, not only in normal physiology, but also in mediating neurotoxicity. Activation of microglia is inherent to multiple neurodegenerative disorders and exposure to toxic compounds. In large measure, these revelations have come about as a result of technologies that enable researchers to obtain high yield and purity primary cultures of rodent microglia. The mechanical isolation protocol discussed in this unit offers an economical method to isolate large amounts of microglia in a short and not too labor‐intensive manner. Most importantly, it ensures a high yield of cells with great reproducibility. Given the ever‐increasing importance of microglia to the field of neurotoxicology research, the ability to isolate large quantities of primary microglia makes it possible to investigate the role and mechanisms associated with microglial modulation of neurotoxicity. We provide a detailed description on the methods that are routinely used in our laboratory for the isolation and culture of microglia, with emphasis on the steps that are deemed most critical for obtaining pure and healthy cultures. Curr. Protoc. Toxicol. 43:12.17.1‐12.17.16. © 2010 by John Wiley & Sons, Inc.
This paper references
Methylmercury causes oxidative stress and cytotoxicity in microglia: Attenuation by 15-deoxy-delta 12, 14-Prostaglandin J2
T. Garg (2006)
Clonal evolution in human lymphoblast cultures.
B. Migeon (1988)
Nanosize Titanium Dioxide Stimulates Reactive Oxygen Species in Brain Microglia and Damages Neurons in Vitro
T. Long (2007)
Increased interleukin-6 expression by microglia from brain of aged mice
S. Ye (1999)
Brain Uptake and Utilization of Fatty Acids, Lipids and Lipoproteins: Application to Neurological Disorders
J. A. Hamilton (2007)
Microglial physiology: unique stimuli, specialized responses.
R. Ransohoff (2009)
Rapid isolation of highly enriched and quiescent microglia from adult rat hippocampus: Immunophenotypic and functional characteristics
M. Frank (2006)
The role of microglia in central nervous system immunity and glioma immunology
I. Yang (2010)
Astrocyte and Oligodendrocyte Cultures
R. Cole (1997)
Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue
K. D. Mccarthy (1980)
Signaling between glia and neurons: focus on synaptic plasticity
N. J. Allen (2005)
Neuroinflammation in Alzheimer's disease and Parkinson's disease: are microglia pathogenic in either disorder?
J. Rogers (2007)
A double staining technique for simultaneous demonstration of astrocytes and microglia in brain sections and astroglial cell cultures.
B. Castellano (1991)
Isolation and characterization of human fetal brain-derived microglia in in vitro culture
N. Hassan (1991)
Mitochondrial-dependent manganese neurotoxicity in rat primary astrocyte cultures
Zhaoobao Yin (2008)
GPCR Signaling Is Required for Blood-Brain Barrier Formation in Drosophila
Tina Schwabe (2005)
Methylmercury causes glial IL-6 release
J. Chang (2007)
Isolation and Flow Cytometric Characterization of Newborn Mouse Brain‐Derived Microglia Maintained In Vitro
N. Hassan (1991)
Microglial activation and its implications in the brain diseases.
S. Dheen (2007)
A Critical Role of Activin A in Maturation of Mouse Peritoneal Macrophages in vitro and in vivo
Y. Wang (2009)
Use of cultured renal tubular cells in the study of cell injury.
Wilson Pd (1986)
Use of cultured renal tubular cells in the study of cell injury.
P. Wilson (1986)
Astrocytes in methylmercury, ammonia, methionine sulfoximine and alcohol-induced neurotoxicity.
M. Aschner (2000)
Activation of microglia by secreted amyloid precursor protein evokes release of glutamate by cystine exchange and attenuates synaptic function
S. Barger (2001)
Co-culture with astrocytes or microglia protects metabolically impaired neurons
L. Park (2001)
Neuron-glia interrelations.
A. Vernadakis (1988)
Isolation and characterization of newborn rabbit brain-derived microglia.
N. Hassan (1991)
Modulation of microglial innate immunity in Alzheimer's disease by activation of peroxisome proliferator-activated receptor gamma.
F. Shie (2009)
rotransmitter receptors on microglia
J. M. Pocock (2007)

This paper is referenced by
Advances in Cellular Models to Explore the Pathophysiology of Amyotrophic Lateral Sclerosis
C. Veyrat-Durebex (2013)
Differential inflammatory response to acrylonitrile in rat primary astrocytes and microglia.
S. Caito (2014)
Methylmercury Alters the Activities of Hsp90 Client Proteins, Prostaglandin E Synthase/p23 (PGES/23) and nNOS
S. Caito (2014)
Microglia demonstrate age-dependent performance and interaction with beta-amyloid peptide
G. Lidónio (2013)
Protective Effect of Semisynthetic and Natural Flavonoid on Aged Rat Microglia–enriched Cultures
Nataša Mrvová (2019)
Methylmercury induces acute oxidative stress, altering Nrf2 protein level in primary microglial cells.
Mingwei Ni (2010)
HAMI 3379, a CysLT2 Receptor Antagonist, Attenuates Ischemia-Like Neuronal Injury by Inhibiting Microglial Activation
Xia-Yan Zhang (2013)
Microglia P2Y13 Receptors Prevent Astrocyte Proliferation Mediated by P2Y1 Receptors
C. Quintas (2018)
Idebenone attenuates cerebral inflammatory injury in ischemia and reperfusion via dampening NLRP3 inflammasome activity.
Jia-Ling Peng (2020)
Cocaine-Mediated Downregulation of miR-124 Activates Microglia by Targeting KLF4 and TLR4 Signaling
P. Periyasamy (2017)
P 2 Y 12 and P 2 Y 13 receptors involved in ADP b s induced the release of IL-1 b , IL-6 and TNF-a from cultured dorsal horn microglia
Pei-Wen Liu (2017)
Developmental Onset of Bilirubin-induced Neurotoxicity Involves Toll-like Receptor 2-dependent Signaling in Humanized UDP-glucuronosyltransferase1 Mice*
Mei-Fei Yueh (2014)
Investigating the regeneration behaviors of neuroglial cells on artificially made electrospun PCL scaffolds embedded with conductive nanomaterials
Madhulika Srikanth (2015)
Fisiopatología del modelo de Theiler: acciones terapéuticas del cannabidiol y efectos protectores en progenitores de oligodendrocitos
Miriam Mecha Rodríguez (2013)
Development and Analysis of Engineered Brain Cell Microenvironments Mimicking Healthy and Diseased Neuronal Circuits
Kahla St. Marthe (2018)
Comparison of the therapeutic effects of bone marrow mononuclear cells and microglia for permanent cerebral ischemia
Chao Jiang (2013)
Loss of Par1b/MARK2 primes microglia during brain development and enhances their sensitivity to injury
Victoria L DiBona (2018)
Human amniotic epithelial stem cells inhibit microglia activation through downregulation of tumor necrosis factor-α, interleukin-1β and matrix metalloproteinase-12 in vitro and in a rat model of intracerebral hemorrhage.
Hongsheng Liang (2014)
Lipid transporter Spns2 promotes microglia pro‐inflammatory activation in response to amyloid‐beta peptide
L. Zhong (2019)
Microglia: Housekeeper of the Central Nervous System
J. Kabba (2017)
Quercetin Protects Obesity-Induced Hypothalamic Inflammation by Reducing Microglia-Mediated Inflammatory Responses via HO-1 Induction
Jihyeon Yang (2017)
Differential response to acrylonitrile toxicity in rat primary astrocytes and microglia.
S. Caito (2013)
Primary microglia isolation from mixed glial cell cultures of neonatal rat brain tissue.
Tami Tamashiro (2012)
Nitric oxide-mediated immunosuppressive effect of human amniotic membrane-derived mesenchymal stem cells on the viability and migration of microglia
K. Yan (2014)
Circumdatin D Exerts Neuroprotective Effects by Attenuating LPS-Induced Pro-Inflammatory Responses and Downregulating Acetylcholinesterase Activity In Vitro and In Vivo
Chan-juan Zhang (2020)
Activating Autophagy in Hippocampal Cells Alleviates the Morphine-Induced Memory Impairment
Jingrui Pan (2016)
P2Y12 and P2Y13 receptors involved in ADPβs induced the release of IL-1β, IL-6 and TNF-α from cultured dorsal horn microglia
Pei-Wen Liu (2017)
Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometry
Belén Calvo (2020)
Microglia demostrate age-dependent performance and interaction with beta-amyloid peptide
Gonçalo Manuel da Costa Lidónio (2013)
Focal Transplantation of Aberrant Glial Cells Carrying the SOD1G93A Mutation into Rat Spinal Cord Induces Extensive Gliosis
Sofía Ibarburu (2017)
Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokines
Paola Pontecorvi (2019)
Microglia change from a reactive to an age-like phenotype with the time in culture
Cláudia Caldeira (2014)
See more
Semantic Scholar Logo Some data provided by SemanticScholar