Online citations, reference lists, and bibliographies.
← Back to Search

In Vivo Response Of GsdmA3Dfl/+ Mice To Topically Applied Fish Oil – Effects On Cellular Markers And Macrophages

M. H. Zulfakar, R. Porter, C. Heard
Published 2016 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Psoriasis is an incurable autoimmune disease characterized by patches of abnormal red, itchy and scaly skin. This work examined the modulation of inflammation, hyperproliferation and immune cell markers following topical application of fish oil (FO) in comparison to the antipsoriatic agents, betamethasone dipropionate (BD) and salicylic acid (SA), to GsdmA3Dfl/+ mice, a hair loss mutant which also exhibits epidermal hyperproliferation akin to psoriasis. The mice were dosed with 100 mg of the test formulation and after 10 days, the mice were sacrificed, skin sections excised and subjected to immunohistochemical determination of COX‐2, K17 and MAC‐1; and immunofluorescence of Ki‐67. Unchanged expression of the proinflammatory enzyme COX‐2 was observed in all treatments, suggesting the noninvolvement of COX‐2 in the aetiology of cutaneous aberration seen in GsdmA3Dfl/+ mice. Intense staining of K17 and MAC‐1 in the FO‐treated group mirrored the epidermal thickening seen observed in live mice by optical coherence tomography (OCT). The ratio of Ki‐67‐positive nuclei per 100 basal cells indicated that hyperproliferation of keratinocytes occurred in FO‐treated mice and the opposite was true for BD‐treated mice. There was a positive correlation (R2 0.995) between Ki‐67 and the epidermal thickness data observed previously. In all immunochemical procedures, the combined BD, SA and FO formulation did not show any significant difference with the control group, reflecting observations seen previously. In conclusion, the epidermal changes observed following topical FO treatment on GsdmA3Dfl/+ mice involves an increase in cellular proliferation and macrophages, although COX‐2 does not appear to play an important role.
This paper references
Effect of fish oil on epidermal hyperproliferation
M H Zulfakar
10.1684/EJD.2007.0201
Is there a role for topically delivered eicosapentaenoic acid in the treatment of psoriasis?
Modh Hanif Zulfakar (2007)
Psoriasis 2 Current and future management of psoriasis
A. Menter (2007)
10.1016/S0090-6980(00)00093-9
Significance of lipoxygenase-derived monohydroxy fatty acids in cutaneous biology.
V. Ziboh (2000)
FEBS Open Bio
(2016)
10.1016/S0753-3322(02)00256-1
Polyunsaturated fatty acids and inflammatory diseases.
Á. Gil (2002)
10.1093/JN/117.8.1360
Dietary influences of evening primrose and fish oil on the skin of essential fatty acid-deficient guinea pigs.
R. Chapkin (1987)
10.1002/jcp.20494
Ki‐67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells
J. Bullwinkel (2006)
10.1111/j.1365-2230.2009.03410.x
A comprehensive review of biomarkers in psoriasis
R. Rashmi (2009)
10.1111/1523-1747.EP12613492
Up-regulation of keratin 17 expression in human HaCaT keratinocytes by interferon-gamma.
B. Bonnekoh (1995)
10.1111/j.1365-2249.1993.tb03377.x
CR3 (CD11b, CD18): a phagocyte and NK cell membrane receptor with multiple ligand specificities and functions
G. Ross (1993)
10.1016/j.neuropharm.2005.10.016
Diclofenac, a non-steroidal anti-inflammatory drug, suppresses apoptosis induced by endoplasmic reticulum stresses by inhibiting caspase signaling
Takao Yamazaki (2006)
10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9
The Ki‐67 protein: From the known and the unknown
T. Scholzen (2000)
10.1002/jcb.10302
Proliferation marker pKi‐67 affects the cell cycle in a self‐regulated manner
M. H. Schmidt (2002)
Keratins ( Kl 6 and Kl 7 ) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro
I Leigh (1995)
10.1111/j.1600-0625.2008.00751.x
Animal models of psoriasis: a critical appraisal
M. Schön (2008)
10.1016/J.PROSTAGLANDINS.2005.12.003
Co-localization of COX-2, CYP4F8, and mPGES-1 in epidermis with prominent expression of CYP4F8 mRNA in psoriatic lesions.
K. Stark (2006)
10.1146/ANNUREV.NUTR.25.050304.092610
Fatty acids as modulators of the immune response.
K. Fritsche (2006)
10.1038/jid.2010.379
Delineating immune-mediated mechanisms underlying hair follicle destruction in the mouse mutant defolliculated.
F. Ruge (2011)
10.1093/ajcn/71.1.361s
Metabolism of polyunsaturated fatty acids by skin epidermal enzymes: generation of antiinflammatory and antiproliferative metabolites.
V. Ziboh (2000)
10.1111/j.1365-2230.1992.tb00194.x
Topical fish oil in psoriasis—a controlled and blind study
S. Escobar (1992)
10.1046/J.1523-1747.1998.00218.X
The relationship between hyperproliferation and epidermal thickening in a mouse model for BCIE.
R. Porter (1998)
10.1046/J.1523-1747.1999.00480.X
Evidence of nuclear PKC/MAP-kinase cascade in guinea pig model of epidermal hyperproliferation.
I. Mani (1999)
10.1007/s00418-008-0435-6
The human keratins: biology and pathology
R. Moll (2008)
10.1111/j.1600-0625.2010.01233.x
In vivo response of GsdmA3Dfl/+ mice to topically applied anti‐psoriatic agents: effects on epidermal thickness, as determined by optical coherence tomography and H&E staining
M. H. Zulfakar (2011)
10.1016/S0140-6736(07)61129-5
Current and future management of psoriasis
A. Menter (2007)
10.1111/J.0022-202X.2005.23623.X
Mutations in gasdermin 3 cause aberrant differentiation of the hair follicle and sebaceous gland.
D. Lunny (2005)
10.1111/j.1365-2133.1995.tb02696.x
Keratins (Kl6 and Kl7) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro
I. Leigh (1995)
834 FEBS Open Bio
(2016)
10.1016/j.jdermsci.2008.04.012
Expression of GLUT-1 in psoriasis and the relationship between GLUT-1 upregulation induced by hypoxia and proliferation of keratinocyte growth.
J. Tao (2008)
10.1111/j.1365-2133.1989.tb01335.x
Topical eicosapentaenoic acid (EPA) in the treatment of psoriasis
Catherine E.Dewsbury (1989)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar