← Back to Search
Entrapment Of Urease In Poly(1‐vinyl Imidazole)/poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic Acid) Network
M. Senel, E. Cevik, M. F. Abasiyanik, A. Bozkurt
Published 2011 · Chemistry
Download PDFAnalyze on Scholarcy
In this article, urease was immobilized in a conducting network via complexation of poly(1-vinyl imidazole) (PVI) with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS). The preparation method for the polymer network was adjusted by using Fourier transform infrared (FTIR) spectroscopy. A scanning electron microscope (SEM) study revealed that enzyme immobilization had a strong effect on film morphology. The proton conductivity of the PVI/PAMPS network was measured via impedance spectroscopy, under humidified conditions. The basic characteristics (Michealis-Menten constants, pHopt, pHstability, Topt, Tstability, reusability, and storage stability) of the immobilized urease were determined. The obtained results showed that the PAA/PVI polymer network was suitable for enzyme immobilization. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
This paper references
Preparation and characterization of urease immobilized on to collagen-poly (glycidyl methacrylate) graft copolymer.
K. Raghunath (1984)
PAMPSA–IM based proton conducting polymer electrolytes
H. Erdemi (2004)
Comparative study between organic and inorganic entrapment matrices for urease biosensor development
A. Maaref (2007)
Recent developments in urea biosensors
Gunjan Dhawan (2009)
Novel conducting polymer electrolyte biosensor based on poly(1-vinyl imidazole) and poly(acrylic acid) networks.
A. Arslan (2006)
A new process for the treatment of fertilizer effluent using immobilized urease
S. George (1997)
Optical fibre biosensors based on immobilised enzymes.
B. Kuswandi (2001)
Polyaniline as a support for urease immobilization
J. Laska (1999)
Proton Conductivity: Materials and Applications
K. Kreuer (1996)
Enzymes and Other Proteins Entrapped in Sol-Gel Materials
D. Avnir (1994)
Urease immobilized on chitosan membrane: preparation and properties.
B. Krajewska (1990)
Preparation and Proton Conductivity of Polymer Electrolytes Based on Alginic Acid and 1,2,4-Triazole
Seyda Tugba Gunday (2008)
Miscibility and interactions in blends of carboxyl-containing polysiloxane with poly(1-vinylimidazole)
Xu Li (2001)
Chitosan-poly (glycidyl methacrylate) copolymer for immobilization of urease
M. Chellapandian (1998)
Preparation and characterization of urease-encapsulated biosensors in poly(vinyl alcohol)-modified silica sol-gel materials.
Hsiao-chung Tsai (2007)
On solids with liquidlike properties and the challenge to develop new proton-conducting separator materials for intermediate-temperature fuel cells.
K. Kreuer (2002)
Immobilization of urease using Amberlite MB-1
A. Anita (1997)
Proton Conducting Blends of Poly(4-vinylimidazole) with Phosphoric Acid
A. Bozkurt (2001)
Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor.
S. Singh (2007)
Urease immobilized on modified polysulphone membrane: preparation and properties.
G. Poźniak (1995)
Novel approaches for the use of mediators in enzyme electrodes.
I. Rosen-Margalit (1993)
Immobilization of urease using glycidyl methacrylate grafted nylon-6-membranes
A. Teke (2007)
A poly(N-isopropylacrylamide-co-N-acryloxysuccinimide-co-2-hydroxyethyl methacrylate) composite hydrogel membrane for urease immobilization to enhance urea hydrolysis rate by temperature swing*
Recent advances in the chemistry of an old enzyme, urease
B. Zerner (1991)
The Synthesis of Complex Polymer Electrolytes Based on Alginic Acid and Poly(1-vinylimidazole) and Application in Tyrosinase Immobilization
M. Kartal (2009)
New Approaches Using Immobilized Enzymes for the Removal of Urea and Ammonia
T. Chang (1980)
Immobilization of urease on gelatin — poly (HEMA) copolymer preparation and characterization
M. Srinivasa Rao (1995)
Entrapment of invertase in an interpenetrated polymer network of alginic acid and poly (1-vinylimidazole)
M. Kartal (2008)
Proton conducting copolymers on the basis of vinylphosphonic acid and 4-vinylimidazole
A. Bozkurt (2003)
Encapsulation of urease enzyme in xanthan-alginate spheres.
Y. M. Elçin (1995)
Preparation and application of spacer-arm-attached poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) films for urease immobilisation
Gulay Bayramoglu (2003)
Anhydrous Protonic Conductivity of a Self-Assembled Acid−Base Composite Material
M. Yamada (2004)
This paper is referenced by
Preparation and conductivities of polyacrylic acid/polyvinylimidazole grafted and ungrafted iron oxide nanocomposite polymer electrolytes
M. Senel (2013)
Novel amperometric glucose biosensor based on covalent immobilization of glucose oxidase on poly(pyrrole propylic acid)/Au nanocomposite
M. Senel (2012)
Synthesis, Chloramphenicol Uptake, and In Vitro Release of Poly(AMPS–TEA-Co-AAm) Gels with Affinity for Both Water and Alcohols
Tingting Weng (2014)
Smart Tandem Catalyst Developed with Sundew's Predation Strategy, Capable of Catching, Decomposing and Assimilating Preys
Panpan Xiao (2018)
An amperometric biosensor based on modified nanoparticles with an electron transfer mediator for the determination of phenol derivatives
E. Cevik (2011)
Entrapment of methyl parathion hydrolase in cross-linked poly(γ-glutamic acid)/gelatin hydrogel.
Jianfei Xie (2014)
Activity and stability of urease entrapped in thermosensitive poly(N-isopropylacrylamide-co-poly(ethyleneglycol)-methacrylate) hydrogel
Gulay Bayramoglu (2013)