Online citations, reference lists, and bibliographies.
← Back to Search

Sequential Morphological Changes Of Erythrocyte Apoptosis In Xenopus Larvae Exposed To 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

M. Sakamoto, S. Mima, T. Kihara, T. Tanimura
Published 2004 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
We previously demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin treatment of Xenopus laevis during the early stages of life induces apoptosis in larval erythrocytes (Sakamoto et al., 1997). In the present study, an examination of these cells at the ultrastructural level was undertaken to elucidate the sequential morphological changes that occur during apoptosis. Xenopus embryos were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin for 5 days shortly after fertilization. The circulating erythrocytes in larvae 12 days after fertilization were examined. Ultramicroscopic studies revealed four roughly defined stages of apoptosis. During the first stage, many small roundish vacuoles begin to appear in the cytoplasm. No noticeable changes can be found in the nucleus. In the second stage, the perinuclear cisterna become dilated, and huge cisternae can be seen in some erythrocytes. The roundish cytoplasmic vacuoles also become larger. Condensation of nuclear chromatin is not yet evident and the erythrocytes still maintain their elliptical shape. During the third stage, chromatin condensation and margination along the nuclear membrane becomes apparent. The nuclear pores gather in the diffuse chromatin region where the perinuclear cisterna is not dilated. The cytoplasm of some erythrocytes also becomes condensed and electron-dense. The normal arrangement of microtubules is disorderly and the erythrocytes deform into a roundish shape. Also, macrophages usually contact some part of the cell. In the final stage, those erythrocytes which show typical nuclear condensation, where neither nuclear or cytoplasmic fragmentation have occurred, are almost or completely phagocytosed by macrophages.
This paper references
10.1096/fasebj.6.6.1312045
The nuclear pore: at the crossroads
J. Hanover (1992)
Metabolic and ultrastructural aspects of the in vitro lysis of chronic lymphocytic leukemia cells by glucocorticoids.
U. Galili (1982)
Perspectives on mammalian cell death.
C. Potten (1987)
10.1016/0045-6535(86)90452-2
Ah receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin: Comparative studies in mammalian and nonmammalian species
M. Denison (1986)
10.1159/000147721
Accumulation and massive cell death of polymorphonuclear neutrophils in the developing bone marrow of the mouse: a histological study.
K. Sasaki (1995)
10.1016/0300-483X(93)90141-E
Early effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on rat thymocytes in vitro.
R. Kurl (1993)
10.1520/STP16766S
Bridging the Gap from Short-Term Teratogenesis Assays to Human Health Hazard Assessment by Understanding Common Modes of Teratogenic Action
J. Bantle (1989)
10.1006/TAAP.1994.1195
Apoptosis: molecular control point in toxicity.
G. Corcoran (1994)
A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy
Mj Karnovsky (1965)
10.1146/ANNUREV.PA.22.040182.002505
2,3,7,8-tetrachlorodibenzo-p-dioxin and related halogenated aromatic hydrocarbons: examination of the mechanism of toxicity.
A. Poland (1982)
10.1177/019262339702500409
Apoptotic Cell Death of Erythrocytes in Xenopus Larvae Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin
M. Sakamoto (1997)
10.1093/TOXSCI/69.1.191
2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in the zebrafish embryo: local circulation failure in the dorsal midbrain is associated with increased apoptosis.
W. Dong (2002)
Death process of primitive erythrocytes and phagocytosis by liver macrophages of the mouse embryo.
K. Sasaki (1997)
Light and electron microscopic observations in Macaca mulatta monkeys fed toxic fat.
J. R. Allen (1967)
10.1007/BF01964378
Innervation of the ovarian interstitial cell of the chick embryo
A. Amanuma (2005)
10.1016/S0006-291X(03)01244-0
Molecular cloning and expression analysis of the aryl hydrocarbon receptor of Xenopus laevis.
H. Ohi (2003)
10.1080/15287398809531120
2,3,7,8-Tetrachlorodibenzo-p-dioxin toxicity in yellow perch (Perca flavescens).
J. Spitsbergen (1988)
Recognition and phagocytosis of apoptotic cells.
L. Dini (1996)
10.1016/S0074-7696(08)62312-8
Cell death: the significance of apoptosis.
A. Wyllie (1980)
10.1016/0092-8674(91)90135-L
Nuclear import-export: In search of signals and mechanisms
E. Nigg (1991)
10.1111/j.1741-4520.1986.tb00655.x
The Xenopus laevis Embryo System for Evaluation of the Developmental Toxicity Using Non‐mammalian Species
Michiko Kamimura (1985)
10.1016/0140-6736(91)91443-X
Neutrophil apoptosis and clearance from neonatal lungs
J. Grigg (1991)
10.1006/FAAT.1997.2331
Perturbation of the mitosis/apoptosis balance: A fundamental mechanism in toxicology
R. Roberts (1997)
Decreased membrane phospholipid packing and decreased cell size precede DNA cleavage in mature mouse B cell apoptosis.
D. A. Mower (1994)
10.1007/BF00048057
Apoptosis and the regulation of cell numbers in normal and neoplastic tissues: an overview
A. Wyllie (2004)
Apoptosis of the intestinal principal cells of Xenopus larvae exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
M. Sakamoto (1999)
Hormone-induced cell death. 2. Surface changes in thymocytes undergoing apoptosis.
R. G. Morris (1984)
10.1679/AOHC.58.205
The fate of effete epithelial cells at the villus tips of the human small intestine.
T. Shibahara (1995)
10.1139/O90-160
The biochemistry of cell death by apoptosis.
W. Bursch (1990)
Production and fate of erythroid cells in anaemic Xenopus laevis.
N. Chegini (1979)
10.1016/0166-445X(91)90027-7
Pathologic alterations in early life stages of lake trout, Salvelinus namaycush, exposed to 2,3,7,8-tetrachlorodibenzo- p-dioxin as
J. Spitsbergen (1991)
A morphological study of liver lesions in Xenopus larvae exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) with special reference to apoptosis of hepatocytes.
M. Sakamoto (1995)
10.1016/S0015-6264(77)80004-7
Morphological changes in monkeys consuming a diet containing low levels of 2,3,7,8-tetrachlorodibenzo-p-dioxin.
J. R. Allen (1977)
10.1007/978-1-4613-3611-2_27
Frog Embryo Teratogenesis Assay: Xenopus (FETAX) — A Short-Term Assay Applicable to Complex Environmental Mixtures
J. Dumont (1983)
10.1002/dvdy.1213
Disruption of erythropoiesis by dioxin in the zebrafish
C. Belair (2001)
Ultrastructural features of apoptosis.
E. Falcieri (1994)
10.1007/BF00158206
Nuclear pores in the apoptotic cell
E. Falcieri (2004)
10.1007/BF00174615
Developmental cell death: morphological diversity and multiple mechanisms
P. G. Clarke (2004)
10.1007/978-1-4899-1462-0_11
Aquatic Toxicity of Dioxins and Related Chemicals
M. K. Walker (1994)
10.1007/BF00342184
Observations on the ultrastructure of nucleated erythrocytes and thrombocytes, with particular reference to the structural basis of their discoidal shape
D. Fawcett (2004)
10.1289/EHP.730587
Toxicology of chlorinated dibenzo-p-dioxins.
B. Schwetz (1973)
Electron microscopic alterations in the liver of chickens fed toxic fat.
J. R. Allen (1966)
10.1152/PHYSREV.1991.71.3.909
Nuclear pore complex: structure, function, and regulation.
M. Miller (1991)
10.1016/S0041-008X(96)80005-6
Embryotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): the embryonic vasculature is a physiological target for TCDD-induced DNA damage and apoptotic cell death in Medaka (Orizias latipes).
S. M. Cantrell (1996)
10.1126/SCIENCE.3262923
2,3,7,8-Tetrachlorodibenzo-p-dioxin kills immature thymocytes by Ca2+-mediated endonuclease activation.
D. McConkey (1988)
10.1111/j.1741-4520.2003.tb01036.x
Zebrafish as a novel experimental model for developmental toxicology
H. Teraoka (2003)
10.1006/TAAP.1997.8309
Correlation of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced apoptotic cell death in the embryonic vasculature with embryotoxicity.
S. M. Cantrell (1998)
10.1016/0041-008X(80)90221-5
Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin in the golden Syrian hamster.
J. Olson (1980)
10.1007/978-94-011-6921-9_2
Cell death: a new classification separating apoptosis from necrosis
A. Wyllie (1981)
10.1083/JCB.11.3.736
A MODIFIED PROCEDURE FOR LEAD STAINING OF THIN SECTIONS
G. Millonig (1961)
10.1016/S0304-3940(01)01743-8
2, 3, 7, 8-tetrachlorodibenzo-p-dioxin induces apoptosis in the dorsal midbrain of zebrafish embryos by activation of arylhydrocarbon receptor
W. Dong (2001)
10.1289/EHP.7305233
Biological responses of the nonhuman primate, chicken, and rat to chlorinated dibenzo-p-dioxin ingestion.
D. Norback (1973)
Effects of continuous exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) from the cleavage to the larval stage on the development of the Xenopus laevis
S. Mima (1997)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar