Online citations, reference lists, and bibliographies.
← Back to Search

Beneficial Effect Of Silkworm Hemolymph On A CHO Cell System: Inhibition Of Apoptosis And Increase Of EPO Production.

S. Choi, W. J. Rhee, T. Park
Published 2005 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
To produce erythropoietin (EPO), Chinese hamster ovary (CHO) cells were first cultured in a medium containing FBS (growth medium) and then in a serum-free medium containing sodium butyrate (production medium). Sodium butyrate increases recombinant protein production, but also induces apoptosis, which reduces cell viability and productivity. In a previous study, we found that silkworm hemolymph (SH), an insect serum, inhibits the apoptosis of insect and mammalian cells. To overcome sodium butyrate-induced apoptosis, we added SH to growth medium. This pretreatment with SH inhibited the sodium butyrate-induced apoptosis of CHO cells and consequently increased their longevity and their ability to produce EPO. As a result, the volumetric productivity of EPO was increased five-fold. SH was found to inhibit cytochrome c release from mitochondria into the cytosol, and prevented the activation of caspase-3 and other subsequent caspase reactions.
This paper references
10.1016/S0092-8674(00)80085-9
Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c
X. Liu (1996)
10.1006/BBRC.2001.5148
Isolation and characterization of an apoptosis-inhibiting component from the hemolymph of Bombyx mori.
E. Kim (2001)
10.1021/bp990093s
Kinetic Effect of Silkworm Hemolymph on the Delayed Host Cell Death in an Insect Cell‐Baculovirus System
Won Jong Rhee (1999)
10.1021/bp0000353
The Growth Factor Inhibitor Suramin Reduces Apoptosis and Cell Aggregation in Protein‐Free CHO Cell Batch Cultures
J. A. Zanghi (2000)
10.1016/S0092-8674(00)80501-2
Apaf-1, a Human Protein Homologous to C. elegans CED-4, Participates in Cytochrome c–Dependent Activation of Caspase-3
H. Zou (1997)
10.1271/bbb.66.2264
Specific Binding of Silkworm Bombyx mori 30-kDa Lipoproteins to Carbohydrates Containing Glucose
M. Ujita (2002)
10.1016/0014-4827(73)90091-8
Morphology and growth rate changes in Chinese hamster cells cultured in presence of sodium butyrate.
J. Wright (1973)
10.1016/S0006-291X(02)00746-5
Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells.
W. J. Rhee (2002)
10.1002/(SICI)1097-0290(20000305)67:5<544::AID-BIT5>3.0.CO;2-#
Part I. Bcl-2 and Bcl-xL limit apoptosis upon infection with alphavirus vectors.
A. Mastrangelo (2000)
Induction of caspase-3 protease activity and apoptosis by butyrate and trichostatin A (inhibitors of histone deacetylase): dependence on protein synthesis and synergy with a mitochondrial/cytochrome c-dependent pathway.
V. Medina (1997)
10.1006/BBRC.1997.7158
Sodium butyrate induces NIH3T3 cells to senescence-like state and enhances promoter activity of p21WAF/CIP1 in p53-independent manner.
H. Xiao (1997)
10.1016/0168-1656(91)90073-5
Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression.
D. P. Palermo (1991)
10.1007/BF02940270
Comparative characterization of growth and recombinant protein production among three insect cell lines with four kinds of serum free media
M. S. Kwon (2003)
10.1016/0005-2795(81)90013-1
Molecular properties and biosynthesis of major plasma proteins in Bombyx mori
Susumu Izumi (1981)
10.1021/bp020001q
Inhibition of Human Cell Apoptosis by Silkworm Hemolymph
S. Choi (2002)
10.1016/S0958-1669(00)00196-8
Understanding and modulating apoptosis in industrial cell culture.
H. A. Laken (2001)
10.1016/S0092-8674(00)81334-3
Human ICE/CED-3 Protease Nomenclature
E. Alnemri (1996)
10.1083/jcb.144.2.281
Ordering the Cytochrome c–initiated Caspase Cascade: Hierarchical Activation of Caspases-2, -3, -6, -7, -8, and -10 in a Caspase-9–dependent Manner
E. Slee (1999)
10.1080/10715769900300091
N-acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells.
Ki Hong Chang (1999)
10.1023/A:1018484309194
Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph
S. Ha (2004)
10.1021/bp034130y
Inhibition of Apoptosis by a Bombyx mori Gene
E. H. Kim (2004)
10.1002/1097-0290(2000)71:3<184::AID-BIT1008>3.0.CO;2-W
Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production.
N. Kim (2000)
10.1016/0092-8674(78)90305-7
Sodium butyrate inhibits histone deacetylation in cultured cells
E. P. Candido (1978)
10.1006/BBRC.2000.2592
Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
W. J. Rhee (2000)
10.1007/BF00174223
Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture
S. Ha (1996)
10.1038/376037A0
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
D. Nicholson (1995)
10.1016/S0092-8674(00)80434-1
Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade
Peng Li (1997)
Flow cytometric analysis of the effect of silkworm hemolymph on the baculovirus-induced insect cell apoptosis
Rhee Won Jong (2001)
10.1016/S0006-291X(03)01425-6
Inhibition of apoptosis by recombinant 30K protein originating from silkworm hemolymph.
E. H. Kim (2003)
10.1007/BF02940260
Anti-apoptosis engineering
Eun Jeong Kim (2003)



This paper is referenced by
10.3390/ijms12074456
Silkworm Hemolymph Down-Regulates the Expression of Endoplasmic Reticulum Chaperones under Radiation-Irradiation
K. Lee (2011)
10.1002/biot.200700249
Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells
C. Mohan (2008)
10.1016/J.PROCBIO.2006.04.010
Enhancement of cell growth and viability of CHO cells in serum-free media by 30Kc6 gene expression
J. Park (2007)
10.1007/978-90-481-2245-5_10
Apoptosis and Autophagy Cell Engineering
C. Mohan (2009)
10.1016/J.MOLCATB.2006.07.013
Enhancement of sialyltransferase-catalyzed transfer of sialic acid onto glycoprotein oligosaccharides using silkworm hemolymph and its 30K protein
S. Choi (2006)
10.1021/bp0702065
Inhibition of HeLa Cell Apoptosis by Storage‐Protein 2
W. J. Rhee (2007)
10.1371/journal.pone.0108761
Crystal Structure of Bombyx mori Lipoprotein 6: Comparative Structural Analysis of the 30-kDa Lipoprotein Family
A. Pietrzyk (2014)
10.1016/J.PROCBIO.2017.08.021
Enzyme delivery using protein-stabilizing and cell-penetrating 30Kc19α protein nanoparticles
Hee Ho Park (2017)
dentification and characterization of a novel cell-penetrating peptide f 30 Kc 19 protein derived from Bombyx mori ee
H. Parka (2014)
Silkworm (Bombyx mori) and its constituents: A fascinating insect in science and research
M. Soumya (2017)
Enhancement of L-Asparaginase Activity in L5178Y Mouse Lymphoma Cells by Conjugating 30Kc19 Protein and L-Asparaginase by Cleavable Linker
S. J. Yang (2016)
10.1016/j.biocel.2020.105717
Protein-based direct reprogramming of fibroblasts to neuronal cells using 30Kc19 protein and transcription factor Ascl1.
Jina Ryu (2020)
10.1016/j.pestbp.2013.10.008
Characteristics of phoxim-exposed gene transcription in the silk gland of silkworms.
L. Ma (2013)
10.1016/J.TIBTECH.2006.01.007
Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells.
T. Kwaks (2006)
10.1186/1471-2164-11-611
The genomic underpinnings of apoptosis in the silkworm, Bombyx mori
Jin-Ye Zhang (2010)
10.1002/BIT.21023
Enhancement of recombinant protein production in Chinese hamster ovary cells through anti‐apoptosis engineering using 30Kc6 gene
S. Choi (2006)
10.1016/J.PROCBIO.2014.05.008
Identification and characterization of a novel cell-penetrating peptide of 30Kc19 protein derived from Bombyx mori
Hee Ho Park (2014)
10.1016/J.PROCBIO.2008.09.018
Beneficial effect of 30Kc6 gene expression on production of recombinant interferon-β in serum-free suspension culture of CHO cells
Tai Young Koo (2009)
10.1007/s00253-014-6045-4
Stabilization of cellular mitochondrial enzyme complex and sialyltransferase activity through supplementation of 30Kc19 protein
J. Park (2014)
Employing epigenetics to augment protein expression in mammalian cells
T. Kwaks (2008)
10.1016/j.biomaterials.2012.08.063
A protein delivery system using 30Kc19 cell-penetrating protein originating from silkworm.
J. Park (2012)
10.1155/2013/323592
Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
W. Yu (2013)
10.1007/s10068-013-0032-x
Antioxidant effect of protein-free silkworm hemolymph extract in mitochondrial membrane potential
S. Choi (2013)
PHARMACEUTICAL MONOCLONAL ANTIBODIES: PRODUCTION, GUIDELINES TO CELL ENGINEERING AND APPLICATIONS
Pankaj Chandel (2013)
10.1002/biot.201400253
Dimerization of 30Kc19 protein in the presence of amphiphilic moiety and importance of Cys-57 during cell penetration
Hee Ho Park (2014)
10.1007/s12257-018-0285-6
Exploring Binding Mechanisms between Curcumin and Silkworm 30Kc19 Protein Using Spectroscopic Analyses and Computational Simulations
Md. Abdur Razzak (2018)
10.1007/s10616-013-9677-9
Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells
Payel Datta (2013)
The Mulberry Silkworm — A New Source of Bioactive Proteins
Wojska Polskiego (2016)
10.1002/biot.201600040
Protein‐stabilizing and cell‐penetrating properties of α‐helix domain of 30Kc19 protein
Jina Ryu (2016)
10.1007/s12257-017-0283-0
Anti-apoptotic effects of the alpha-helix domain of silkworm storage protein 1
Ji Eun Baik (2017)
10.1016/j.biomaterials.2013.11.001
Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles.
Hong Jai Lee (2014)
10.1007/S12257-009-0081-4
Expression of Bombyx mori 30Kc19 protein in Escherichia coli and its anti-apoptotic effect in Sf9 cell
W. J. Rhee (2009)
See more
Semantic Scholar Logo Some data provided by SemanticScholar