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Development And Validation Of A Rapid Reversed-phase HPLC Method For The Determination Of Insulin From Nanoparticulate Systems.

B. Sarmento, A. Ribeiro, F. Veiga, D. Ferreira
Published 2006 · Chemistry, Medicine

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A reversed-phase high-performance liquid chromatographic (HPLC) method has been developed and validated for the determination of insulin in nanoparticulate dosage forms. Its application for the development and characterization of insulin-loaded nanoparticulates composed of polyelectrolytes has also been carried out. A reversed-phase (RP) C18 column and gradient elution with a mobile phase composed of acetonitrile (ACN) and 0.1% aqueous trifluoroacetic acid (TFA) solution at a flow rate of 1 mL/min was used. Protein identification was made by UV detection at 214 nm. The gradient changed from 30:70 (ACN:TFA, v/v) to 40:60 (v/v) in 5 min followed by isocratic elution at 40:60 (v/v) for a further five minutes. The method was linear in the range of 1-100 microg/mL (R2 = 0.9996), specific with a good inter-day and intra-day precision based on relative standard deviation values (less than 3.80%). The recovery was between 98.86 and 100.88% and the detection and quantitation limits were 0.24 and 0.72 microg/mL, respectively. The method was further tested for the determination of the association efficiency of insulin to nanoparticulate carriers composed of alginate and chitosan, as well as its loading capacity for this protein. Encapsulant release under simulated gastrointestinal fluids was evaluated. The method can be used for development and characterization of insulin-loaded nanoparticles made from cross-linked chitosan-alginate.
This paper references
High-performance liquid chromatographic determination of insulin in rat and human plasma.
G. Khaksa (1998)
A rapid and sensitive method for simultaneous determination of insulin and A21-desamido insulin by high-performance liquid chromatography.
Payam Moslemi (2003)
Quantitation of insulin by capillary electrophoresis and high-performance liquid chromatography method comparison and validation
Annette Kunkel (1997)
Development of two high-performance liquid chromatographic methods for the analysis and characterization of insulin and its degradation products in pharmaceutical preparations.
A. Oliva (2000)
A novel sustained-release formulation of insulin with dramatic reduction in initial rapid release.
M. Takenaga (2002)
duction of chitosan - coated insulin - loaded alginate nanoparticles by ionotropic gelation
B Sarmento (2004)
HPLC of peptides and proteins: basic theory and methodology.
M. Aguilar (2004)
Production of chitosan-coated insulin-loaded alginate nanoparticles by ionotropic gelation
B Sarmento (2004)
Chitosan/calcium–alginate beads for oral delivery of insulin
P. Hari (1996)
duction of chitosan - coated insulin - loaded alginate nanoparticles by ionotropic gelation
B Sarmento (2004)
Protein immobilization in crosslinked alginate microparticles
G. Coppi (2002)
Oral peptide drug delivery: polymer-inhibitor conjugates protecting insulin from enzymatic degradation in vitro.
M. Marschütz (2000)
Validation of liquid chromatographic and gas chromatographic methods. Applications to pharmacokinetics.
F. Bressolle (1996)
Insulinloaded alginate/chitosan nanoparticles produced by ionotropic pregelation
B Sarmento (2005)
HPLC of Peptides and Proteins
M. Aguilar (2003)
Encapsulation of insulin in chitosan-coated alginate beads: oral therapeutic peptide delivery.
S. Onal (2002)
Insulin‐loaded nanocapsules for oral administration: In vitro and in vivo investigation
M. Aboubakar (2000)
Evaluation of protein release from chitosan-alginate microcapsules produced using external or internal gelation.
G. Vandenberg (2001)
Validation of HPLC Techniques for Pharmaceutical Analysis
N. Épshtein (2004)
The Effect of Site of Administration in the Gastrointestinal Tract on the Absorption of Insulin from Nanocapsules in Diabetic Rats
C. Michel (1991)
Toward understanding insulin fibrillation.
J. Brange (1997)

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