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A Substrate‐Independent Approach For The Surface Immobilization Of Oligonucleotides Using Aldehyde Functionalized Surfaces

J. McGettrick, W. E. Schofield, R. P. Garrod, J. P. Badyal
Published 2009 · Materials Science

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A highly efficient approach for the covalent immobilization of single-strand DNA onto solid surfaces and their subsequent hybridization to complementary strands is described. This entails pulsed plasma deposition of poly(3-vinylbenzaldehyde) followed by the Schiff-base reaction. A range of substrates, including glass slides, silicon wafers, polystyrene microspheres, and single-walled carbon nanotubes (SWNTs), are functionalized employing this methodology.
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