Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Stimulation Of Protein And DNA Synthesis In Mouse C2C12 Satellite Cells: Evidence For Phospholipase D‐dependent And ‐independent Pathways

K. S. Morrison, S. Mackie, R. M. Palmer, M. Thompson
Published 1995 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
In C2C12 myoblasts, 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) stimulated a phospholipase D (PLD) to degrade phosphatidylcholine (PC) as measured by the release of choline and an increase in the formation of phosphatidic acid (PA) (or phosphatidylbutanol [PtdBuOH] in the presence of 0.5% butanol). Exogenous PLD also stimulated choline release, PA and PtdBuOH formation. The protein kinase C (PKC) inhibitor, Ro‐31‐8220, and PKC downregulation significantly inhibited the effects of TPA but Ro‐31‐8220 had no effect on PLD action. Neither basic Fibroblast Growth Factor (bFGF) or Epidermal Growth Factor (EGF) increased PLD activity. All agonists stimulated protein synthesis during both a 90 min and a 6 hr incubation and increased RNA accretion after 6 hr. The response at 90 min was not inhibited by the transcription inhibitor, actinomycin D. Ro‐31‐8220 and PKC downregulation significantly inhibited all the effects of TPA. In contrast, Ro‐31‐8220 significantly inhibited the increase in RNA accretion elicited by PLD but had no effect on the ability of agonists other than TPA to enhance protein synthesis. All agonists also stimulated thymidine incorporation into DNA. The effects of EGF, bFGF, and PLD were rapid and transient whereas that of TPA was delayed and sustained. Ro‐31‐8220 and PKC downregulation significantly inhibited the response due to TPA. Furthermore, Ro‐31‐8220 also significantly inhibited the effects elicited by EGF and PLD but not that induced by bFGF. In differentiated myotubes, TPA and PLD, but not bFGF or EGF, again stimulated choline release and PtdBuOH formation. However, all agents failed to stimulate protein synthesis and RNA accretion. The data demonstrate the presence in C2C12 myoblasts, but not differentiated myotubes, of both a PLD‐dependent and PLD‐independent pathway(s) leading to the stimulation of protein synthesis, RNA accretion, and DNA synthesis. © 1995 Wiley‐Liss, Inc.
This paper references
10.1042/BJ2700427
Stimulation of insulin release by phospholipase D. A potential role for endogenous phosphatidic acid in pancreatic islet function.
S. Metz (1990)
10.1016/s0021-9258(19)38335-8
Lysophosphatidic acid, but not phosphatidic acid, is a potent Ca2(+)-mobilizing stimulus for fibroblasts. Evidence for an extracellular site of action.
K. Jalink (1990)
10.1016/0005-2760(84)90102-4
Ca2+-dependent conversion of phosphatidylinositol to phosphatidate in neutrophils stimulated with fMet-Leu-Phe or ionophore A23187.
S. Cockcroft (1984)
10.1002/JCP.1041400103
Dexamethasone effects on creatine kinase activity and insulin‐like growth factor receptors in cultured muscle cells
P. Whitson (1989)
10.1002/MUS.880040311
Skeletal muscle regeneration
D. Allbrook (1981)
10.1042/BJ2760019
Angiotensin II-induced phosphatidylcholine hydrolysis in cultured vascular smooth-muscle cells. Regulation and localization.
B. Lassègue (1991)
10.1016/0167-4889(90)90009-3
Phosphatidylcholine hydrolysis stimulated by phorbol myristate acetate is mediated principally by phospholipase D in endothelial cells.
T. W. Martin (1990)
10.1016/0163-7827(90)90001-2
Effects of phorbol ester on phospholipid metabolism.
Z. Kiss (1990)
10.1021/BI00524A057
Enhancement of calcium uptake and phosphatidylinositol turnover by epidermal growth factor in A-431 cells.
S. Sawyer (1981)
10.1038/270725a0
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle
D. Yaffe (1977)
10.1152/AJPCELL.1994.266.1.C206
Basic FGF activates phospholipase D in endothelial cells in the absence of inositol-lipid hydrolysis.
A. Ahmed (1994)
10.1042/BJ2870051
Epidermal-growth-factor-induced production of phosphatidylalcohols by HeLa cells and A431 cells through activation of phospholipase D.
M. Kaszkin (1992)
10.1126/SCIENCE.2237442
A cytoplasmic protein inhibits the GTPase activity of H-Ras in a phospholipid-dependent manner.
M. H. Tsai (1990)
10.1038/322644A0
Loss of genes on chromosome 22 in tumorigenesis of human acoustic neuroma
B. Seizinger (1986)
10.1083/JCB.107.2.761
Cell surface fibroblast growth factor and epidermal growth factor receptors are permanently lost during skeletal muscle terminal differentiation in culture
B. Olwin (1988)
10.1001/ARCHNEUR.1985.04210090013004
Synergistic influence of polypeptide growth factors on cultured human muscle.
V. Askanas (1985)
Formation of skeletal muscle in vivo from the mouse C2 cell line.
J. Morgan (1992)
10.1677/JOE.0.1270039
Basic fibroblast growth factor affects DNA synthesis and cell function and activates multiple signalling pathways in rat thyroid FRTL-5 and pituitary GH3 cells.
E. Black (1990)
10.1128/MCB.6.5.1412
Transcriptional and posttranscriptional control of c-myc during myogenesis: its mRNA remains inducible in differentiated cells and does not suppress the differentiated phenotype.
T. Endo (1986)
10.1038/332644A0
Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate
M. Whitman (1988)
10.2527/1991.691146X
Growth factor regulation of bovine satellite cell growth in vitro.
E. A. Greene (1991)
10.1016/0012-1606(71)90017-0
Alterations of enzymatic activities during muscle differentiation in vitro.
A. Shainberg (1971)
10.1093/OXFORDJOURNALS.JBCHEM.A132334
Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose.
S. Imamura (1979)
10.1016/s0021-9258(19)68537-6
Stimulation of phosphatidic acid of calcium influx and cyclic GMP synthesis in neuroblastoma cells.
S. Ohsako (1981)
10.1083/JCB.98.2.739
A rapid decrease in epidermal growth factor-binding capacity accompanies the terminal differentiation of mouse myoblasts in vitro
R. Lim (1984)
10.1016/0014-5793(89)81494-2
Potent selective inhibitors of protein kinase C
P. Davis (1989)
10.1042/BJ2690281
The regulation and cellular functions of phosphatidylcholine hydrolysis.
M. Billah (1990)
10.1016/0005-2760(94)90186-4
Phosphatidylcholine breakdown and signal transduction.
J. Exton (1994)
10.1016/0277-5379(91)90094-T
Doxorubicin interactions at the membrane: evidence for a biphasic modulation of inositol lipid metabolism.
M. Thompson (1991)
10.1016/s0021-9258(18)45603-7
Phosphatidic acid may stimulate membrane receptors mediating adenylate cyclase inhibition and phospholipid breakdown in 3T3 fibroblasts.
T. Murayama (1987)
10.1073/PNAS.88.14.6210
Phosphatidate-dependent protein phosphorylation.
S. Bocckino (1991)
10.1016/0022-510X(92)90149-F
Effects of leukaemia inhibitory factor and other cytokines on murine and human myoblast proliferation
L. Austin (1992)
10.1016/s0021-9258(18)77324-9
Phosphatidic acid modulates DNA synthesis, phospholipase C, and platelet-derived growth factor mRNAs in cultured mesangial cells. Role of protein kinase C.
T. Knauss (1990)
10.1002/JCP.1041390315
Second messenger function of phosphatidic acid in platelet activation
M. Kroll (1989)
10.1016/s0021-9258(19)76551-x
Kinetic analysis of 1,2-diacylglycerol mass levels in cultured fibroblasts. Comparison of stimulation by alpha-thrombin and epidermal growth factor.
T. M. Wright (1988)
10.1016/0014-5793(89)81340-7
Stimulation of polyphosphoinositide hydrolysis in Swiss 3T3 cells by recombinant fibroblast growth factors
K. D. Brown (1989)
10.1016/s0021-9258(18)35882-4
Phospholipase D mimics platelet-derived growth factor as a competence factor in vascular smooth muscle cells.
T. Kondo (1992)
10.1016/0092-8674(90)90074-O
Phospholipase C-mediated hydrolysis of phosphatidlycholine is an important step in PDGF-stimulated DNA synthesis
P. Larrodera (1990)
10.1042/BJ2810163
Mitogenic action of lysophosphatidic acid and phosphatidic acid on fibroblasts. Dependence on acyl-chain length and inhibition by suramin.
E. V. van Corven (1992)
10.1152/AJPCELL.1992.262.6.C1376
Norepinephrine-induced phosphatidylcholine hydrolysis by phospholipases D and C in rat tail artery.
H. Gu (1992)
10.1042/BJ2700737
Inhibitors of phospholipase A2 block the stimulation of protein synthesis by insulin in L6 myoblasts.
B. G. Southorn (1990)
10.1006/EXCR.1993.1199
The role of macrophages in skeletal muscle regeneration with particular reference to chemotaxis.
T. Robertson (1993)
10.1002/JCP.1041380213
Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor‐beta, insulin‐like growth factor I, and fibroblast growth factor
R. Allen (1989)
10.1016/0167-4889(94)90191-0
Stimulation of protein synthesis and phospholipase D activity by vasopressin and phorbol ester in L6 myoblasts.
M. Thompson (1994)
10.1016/s0021-9258(18)41863-7
Identification of phosphatidylcholine-selective and phosphatidylinositol-selective phospholipases D in Madin-Darby canine kidney cells.
C. Huang (1992)
Gonadotropin-releasing hormone activates phospholipase D in ovarian granulosa cells. Possible role in signal transduction.
M. Liscovitch (1989)
10.1073/PNAS.88.2.598
Overexpression of protein kinase C beta 1 enhances phospholipase D activity and diacylglycerol formation in phorbol ester-stimulated rat fibroblasts.
J. Pai (1991)
10.1016/0092-8674(88)90531-4
Cellular ras activity and phospholipid metabolism
C. Yu (1988)
10.1126/SCIENCE.3014651
Studies and perspectives of protein kinase C.
Y. Nishizuka (1986)
10.1016/s0021-9258(18)48176-8
Phosphatidate accumulation in hormone-treated hepatocytes via a phospholipase D mechanism.
S. Bocckino (1987)
10.1016/0014-5793(93)81440-B
Enhancement of phospholipase A2 activation by phosphatidic acid endogenously formed through phospholipase D action in rat peritoneal mast cell
T. Sato (1993)
10.1016/s0021-9258(18)42502-1
Activation of phospholipase D by protein kinase C. Evidence for a phosphorylation-independent mechanism.
K. M. Conricode (1992)
10.1038/312315A0
Inositol trisphosphate, a novel second messenger in cellular signal transduction
M. Berridge (1984)
10.1007/BF01150480
Arachidonate activation of protein kinase C may be involved in the stimulation of protein synthesis by insulin in L6 myoblasts
M. Thompson (1993)
10.1146/ANNUREV.BI.56.070187.004313
Receptors for epidermal growth factor and other polypeptide mitogens.
G. Carpenter (1987)
10.1016/s0021-9258(18)81903-2
Regulation of phospholipase D in HL-60 granulocytes. Activation by phorbol esters, diglyceride, and calcium ionophore via protein kinase- independent mechanisms.
M. Billah (1989)
10.1083/JCB.111.4.1623
Regulation of proliferation and differentiation of myoblasts derived from adult mouse skeletal muscle by specific isoforms of PDGF
Z. Yablonka-Reuveni (1990)
10.1038/323171A0
Growth factor-like action of phosphatidic acid
W. Moolenaar (1986)
10.1126/SCIENCE.2536192
The effect of GTPase activating protein upon ras is inhibited by mitogenically responsive lipids.
M. H. Tsai (1989)



This paper is referenced by
10.1152/AJPGI.00041.2006
Phospholipase D and extracellular signal-regulated kinase in hepatic stellate cells: effects of platelet-derived growth factor and extracellular nucleotides.
Joaquín Benítez-Rajal (2006)
10.1016/j.bbalip.2009.04.007
The roles of phospholipase D in EGFR signaling.
C. Lee (2009)
Effect of a cancer cachectic factor on protein synthesis/degradation in murine C2C12 myoblasts: modulation by eicosapentaenoic acid.
H. J. Smith (1999)
10.1152/AJPCELL.1996.270.6.C1875
N tau-methylhistidine turnover in skeletal muscle cells measured by GC-MS.
M. Thompson (1996)
10.1007/978-1-4939-0464-8_22
Emerging Roles of Phospholipase D in Pathophysiological Signaling
C. Lee (2014)
10.1074/JBC.272.16.10910
Regulation of Phospholipase D in L6 Skeletal Muscle Myoblasts
M. Thompson (1997)
10.1079/BJN19980059
Growth and metabolism of fetal and maternal muscles of adolescent sheep on adequate or high feed intake: possible role of protein kinase C-alpha in fetal muscle growth.
R. M. Palmer (1998)
10.1002/(SICI)1097-4652(199603)166:3<506::AID-JCP5>3.0.CO;2-T
Measurement of protein degradation by release of labelled 3‐methylhistidine from skeletal muscle and non‐muscle cells
M. Thompson (1996)
Modulation by Eicosapentaenoic Acid Myoblasts : 12 C 2 Synthesis / Degradation in Murine C Effect of a Cancer Cachectic Factor on Protein Updated
Helen J. Smith (1999)
10.1016/S0005-2760(98)00013-7
A role for phospholipase D activation in the lipid signalling cascade generated by bradykinin and thrombin in C2C12 myoblasts.
V. Vasta (1998)
Semantic Scholar Logo Some data provided by SemanticScholar