Online citations, reference lists, and bibliographies.
← Back to Search

31P NMR Spectroscopy Of 9L Cell Line In Culture: Differential Effects Of High Temperature On Anchored Cells And Spheroids

N. Suzuki, I. Kwee, L. B. Tamas, T. Nakada
Published 1991 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The differential effects of high temperature on 9L rat gliosarcoma cells cultured in two distinctive forms, namely, anchored monolayer cells and multicellular spheroids, were investigated using 31P nuclear magnetic resonance spectroscopy. The critical temperatures resulting in irreversible changes in cellular energetics were found to be significantly different: 45°C for anchored cells and 43°C for multicellular spheroids, respectively. Phosphonionoester levels in multicellular spheroids were found to be consistently higher than those in anchored monolayer cells regardless of the temperature to which they were exposed. The study demonstrates that tissue derived from a single cell line may exhibit different metabolic properties depending on its growth pattern. © 1991 Academic Press, Inc.
This paper references
10.1016/S0167-8140(84)80077-8
The relevance of tumour pH to the treatment of malignant disease.
J. Wike-Hooley (1984)
In vivo growth of tumor cell spheroids after in vitro hyperthermia.
J. Landry (1982)
10.1002/IJC.2910390222
Growth of a human colonic adenocarcinoma cell line (HT 29) on microcarrier beads: Metabolic studies by 31phosphorus nuclear magnetic resonance spectroscopy
Jacques Fantini Baetselier (1987)
Measurements of in vivo 31P nuclear magnetic resonance spectra in neuroectodermal tumors for the evaluation of the effects of chemotherapy.
S. Naruse (1985)
10.2307/3576758
Comparative study of the effects of hyperthermia and BCNU on BCNU-sensitive and BCNU-resistant 9L rat brain tumor cells.
V. D. da Silva (1985)
10.1083/JCB.95.1.24
31P nuclear magnetic resonance evidence for the regulation of intracellular pH by Ehrlich ascites tumor cells
R. Gillies (1982)
Response of solid tumors to chemotherapy monitored by in vivo 31P nuclear magnetic resonance spectroscopy: a review.
Steen Rg (1989)
10.1016/0014-5793(84)81257-0
Phosphorylethanolamine ‐ the major constituent of the phosphomonoester peak observed by 31P‐NMR on developing dog brain
L. Gyulai (1984)
10.1126/SCIENCE.2451290
Cell and environment interactions in tumor microregions: the multicell spheroid model.
R. M. Sutherland (1988)
10.1002/MRM.1910060102
A versatile multinuclear probe designed for in vivo NMR spectroscopy: Applications to subcutaneous human tumors in mice
R. Lyon (1988)
10.1002/MRM.1910030503
A perfusion technique for 13C NMR studies of the metabolism of 13C‐labeled substrates by mammalian cells
R. Lyon (1986)
10.1073/PNAS.78.8.4843
31P NMR studies of intact anchorage-dependent mouse embryo fibroblasts.
K. Uǧurbil (1981)
10.1093/JNCI/64.6.1373
Development of a 9L rat brain tumor cell multicellular spheroid system and its response to 1,3-bis(2-chloroethyl)-1-nitrosourea and radiation.
D. Deen (1980)
10.1148/RADIOLOGY.137.2.7433686
Effects of hyperthermia on normal and tumor microenvironment.
H. Bicher (1980)
10.1002/MRM.1910010410
Application ofin vivo NMR spectroscopy to cancer
W. Evanochko (1984)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar