Please confirm you are human (Sign Up for free to never see this)
← Back to Search
A New Stain For Quantitative Measurement Of Sprouting At Neuromuscular Junctions
A. Pestronk, D. Drachman
Published 1978 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
A new combined stain for the simultaneous demonstration of motor nerve terminals and cholinesterase at neuromuscular junctions is described. It employs bromoindoxyl acetate dye‐staining for cholinesterase and silver‐gold impregnation for nerve terminals. The clarity and reliability of the stain permit quantitative measurements of neuromuscular junctions in order to evaluate nerve terminal sprouting as well as other pathological changes. The method is rapid, reproducible, and simple, and it is well suited for the processing of large numbers of frozen sections.
This paper references
The changes in the motor end-plate in myasthenia gravis.
V. MacDermot (1960)
A modified chrome-silver paraffin wax technique for staining neural end-feet.
J. Armstrong (1960)
The intramuscular nerve endings in myasthenia gravis.
E. Bickerstaff (1960)
Trophic regulation of nerve sprouting.
J. Diamond (1976)
Re-innervation of paretic muscle by collateral branching of the residual motor innervation.
A. Harreveld (1952)
Regeneration in Duchenne muscular dystrophy. Electromyographic evidence.
J. Desmedt (1976)
Terminal innervation ratio in neuromuscular disease. I. Methods and controls.
C. Coërs (1973)
An electron microscopic study of the changes induced by botulinum toxin in the motor end-plates of slow and fast skeletal muscle fibres of the mouse.
L. Duchen (1971)
A method for obtaining longitudinal cryostat sections of living muscle without contraction artifacts.
J. Pearson (1974)
A new method for staining nerve fibers and nerve endings in mounted paraffin sections
D. Bodian (1936)
Collateral Nerve Regeneration
M. V. Edds (1953)
Sprouting and degeneration of mammalian motor axons in normal and de-afferentated skeletal muscle
D. Barker (1966)
Degeneration and regeneration of the nervous system
M. Scheibel (1960)
The histology of the neuromuscular junction in dystrophia myotonica.
V. MacDermot (1961)
Cytochemical Localization of Esterases Using Indoxyl Derivatives
S. Holt (1952)
Complete staining of neuromuscular innervation with bromoindigo and silver.
G. McIsaac (1974)
Collateral regeneration in partially denervated muscles
G. Wohlfart (1958)
A rapid method for demonstrating skeletal muscle motor innervation in frozen sections.
J. Toop (1976)
Local re-innervation in partially denervated muscle; a histophysiological study.
H. Hoffman (1950)
Detection of light‐induced changes of intracellular ionized calcium concentration in Limulus ventral photoreceptors using arsenazo III
J. E. Brown (1977)
This paper is referenced by
Lengthening extraocular muscle with autologous muscle transplants.
S. Christiansen (1994)
The location of neuromuscular junctions on regenerating adult mouse muscle in culture
M. Ecob (1984)
Ectopic endplates induce localized changes in skeletal muscle ultrastructure
D. Bishop (2002)
Attenuation of botulinum toxin mediated blockade of exocytosis at neuromuscular junctions by viral vector mediated gene transfer into spinal motor neurons
A. Raghunath (2009)
Overexpression of the neural growth-associated protein GAP-43 induces nerve sprouting in the adult nervous system of transgenic mice
L. Aigner (1995)
Exrraocular Muscle Regeneration in Freeze-Treated Extraocular Muscle Autograffs
Robert W. Baker (2005)
PDFlib PLOP: PDF Linearization, Optimization, Protection Page inserted by evaluation version
J. E. Yeo (2001)
IGF-1 gene transfer into denervated rat laryngeal muscle.
P. Flint (1999)
Surgical reneurotization of the tongue after unilateral paralysis of the hypoglossal nerve
W. Hosemann (2004)
Paucity of secondary synaptic clefts in a case of congenital myasthenia with multiple contractures: ultrastructural morphology of a developmental disorder.
L. M. Smit (1984)
Overexpression of GDNF Induces and Maintains Hyperinnervation of Muscle Fibers and Multiple End-Plate Formation
M. Zwick (2001)
Morphological and electrophysiological study of distal motor nerve fiber degeneration and sprouting after irreversible cholinesterase inhibition
M. Kawabuchi (1991)
Effects of aging on nerve sprouting and regeneration
A. Pestronk (1980)
Nerve sprouting and endplate growth induced in normal muscle by contralateral partial denervation of rat plantaris
B. Pachter (1991)
The anatomy of the muscle insertion (scleromuscular junction) of the lateral and medial rectus muscle in humans.
G. Jaggi (2005)
β-Endorphin and corticotropin immunoreactivity and specific binding in the neuromuscular system of obese-diabetic mice
S. Hughes (1992)
Histochemical staining of the acetylcholine receptor, acetylcholinesterase, and the axon terminal
E. K. Bjornskov (1982)
Idiopathic peripheral neuropathy in the horse with knuckling: muscle and nerve lesions in additional cases
H. Furuoka (1998)
Cytoplasm-to-myonucleus ratios and succinate dehydrogenase activities in adult rat slow and fast muscle fibers
B. Tseng (2004)
Proopiomelanocortin-derived peptides in mice with motoneurone disease
S. Hughes (1989)
The Innervation of Muscle Spindles in an Intrinsic Muscle of the Hind Foot: The Superficial Lumbrical of the Cat
R. Banks (1995)
Development of a Regenerative Peripheral Nerve Interface for Control of a Neuroprosthetic Limb
M. Urbanchek (2016)
Role of muscle insulin-like growth factors in nerve sprouting: suppression of terminal sprouting in paralyzed muscle by IGF-binding protein 4
P. Caroni (1994)
A myasthenic syndrome with congenital paucity of secondary synaptic clefts: CPSC Syndrome
L. M. Smit (1988)
Imaging neuromuscular junctions by confocal fluorescence microscopy: individual endplates seen in whole muscles with vital intracellular staining of the nerve terminals
M. Marques (1998)
Light- and electron-microscopical study of phosphoprotein B-50 following denervation and reinnervation of the rat soleus muscle
J. Verhaagen (1988)
Schwartz–Jampel syndrome: I. Clinical, electromyographic, and histologic studies
F. Spaans (1990)
The cellular events of injured muscle regeneration depend on the nature of the injury
J. P. Lefaucheur (1995)
Reinnervation of a denervated skeletal muscle by spinal axons regenerating through a collagen channel directly implanted into the rat spinal cord
L. Kassar-Duchossoy (2001)
Type-specific changes in fiber morphometry following denervation of canine extraocular muscle.
S. Christiansen (1992)
Motor nerve outgrowth: reduced capacity for sprouting in the terminals of longer axons
A. Pestronk (1988)
Intrinsic Neuronal Determinants Locally Regulate Extrasynaptic and Synaptic Growth at the Adult Neuromuscular Junction
P. Caroni (1997)See more