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Engineering 3D Hydrogels For Personalized In Vitro Human Tissue Models.
Chya-Yan Liaw, S. Ji, M. Guvendiren
Published 2018 · Chemistry, Medicine
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There is a growing interest in engineering hydrogels for 3D tissue and disease models. The major motivation is to better mimic the physiological microenvironment of the disease and human condition. 3D tissue models derived from patients' own cells can potentially revolutionize the way treatment and diagnostic alternatives are developed. This requires development of tissue mimetic hydrogels with user defined and tunable properties. In this review article, a recent summary of 3D hydrogel platforms for in vitro tissue and disease modeling is given. Hydrogel design considerations and available hydrogel systems are summarized, followed by the types of currently available hydrogel models, such as bulk hydrogels, porous scaffolds, fibrous scaffolds, hydrogel microspheres, hydrogel sandwich systems, microwells, and 3D bioprinted constructs. Although hydrogels are utilized for a wide range of tissue models, this article focuses on liver and cancer models. This article also provides a detailed section on current challenges and future perspectives of hydrogel-based tissue models.
This paper references
Advancing the understanding of autism disease mechanisms through genetics
L. torre-Ubieta (2016)
Cell-laden microengineered gelatin methacrylate hydrogels.
J. W. Nichol (2010)
Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels
Valerie Liu Tsang (2007)
3D bioprinted glioma stem cells for brain tumor model and applications of drug susceptibility.
Xingliang Dai (2016)
Fabrication of viable tissue-engineered constructs with 3D cell-assembly technique.
Yongnian Yan (2005)
Conservation and novelty in the evolution of cell adhesion and extracellular matrix genes.
H. Hutter (2000)
Viscoelastic lithography for fabricating self-organizing soft micro-honeycomb structures with ultra-high aspect ratios
G. S. Jeong (2016)
Cellular Traction Stresses Increase with Increasing Metastatic Potential
Casey M. Kraning-Rush (2012)
Design of Thiol- and Light-sensitive Degradable Hydrogels using Michael-type Addition Reactions.
Prathamesh M Kharkar (2015)
The extracellular matrix at a glance
Christian Frantz (2010)
Bioengineering 3D environments for cancer models.
Mireia Alemany-Ribes (2014)
Engineered silk fibroin protein 3D matrices for in vitro tumor model.
Sarmistha Talukdar (2011)
Sandwich-cultured hepatocytes: an in vitro model to evaluate hepatobiliary transporter-based drug interactions and hepatotoxicity
B. Swift (2010)
Location, location, location: Cell‐level mechanics in liver fibrosis
R. Wells (2016)
Application areas of 3D bioprinting.
Ibrahim T. Ozbolat (2016)
Genomic responses in mouse models poorly mimic human inflammatory diseases
Keizo Takao (2013)
The extracellular matrix and insulin resistance
A. S. Williams (2015)
Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
Tao Jiang (2017)
The role of matrix stiffness in regulating cell behavior
R. Wells (2008)
A multicellular 3D heterospheroid model of liver tumor and stromal cells in collagen gel for anti-cancer drug testing.
Derek Yip (2013)
Flow–perfusion bioreactor system for engineered breast cancer surrogates to be used in preclinical testing
L. Marshall (2017)
Mechanics, malignancy, and metastasis: The force journey of a tumor cell
S. Kumar (2008)
Matrix Elasticity Directs Stem Cell Lineage Specification
A. Engler (2006)
Relationship between hyaluronan production and metastatic potential of mouse mammary carcinoma cells.
N. Itano (1999)
Overproduction of hyaluronan by expression of the hyaluronan synthase Has2 enhances anchorage-independent growth and tumorigenicity.
R. Kosaki (1999)
3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained
Michele Zanoni (2016)
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi (2007)
Isolation and co-culture of rat parenchymal and non-parenchymal liver cells to evaluate cellular interactions and response
S. Bale (2016)
Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering.
JunMin Zhu (2010)
Nuclear Lamin-A Scales with Tissue Stiffness and Enhances Matrix-Directed Differentiation
Joe Swift (2013)
Hyaluronan synthase 3 overexpression promotes the growth of TSU prostate cancer cells.
N. Liu (2001)
Hepatocytes cultured in alginate microspheres: an optimized technique to study enzyme induction.
M. Ringel (2005)
Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors
A. Jacobson (2002)
Novel 3D Co-Culture Model for Epithelial-Stromal Cells Interaction in Prostate Cancer
Xiaolan Fang (2013)
Bioprinting for cancer research.
S. Knowlton (2015)
An overview of inverted colloidal crystal systems for tissue engineering.
C. Filipe (2014)
3-D tumor model for in vitro evaluation of anticancer drugs.
Jayme L Horning (2008)
Assessment of hepatocellular function within PEG hydrogels.
G. H. Underhill (2007)
Current and emerging applications of 3D printing in medicine.
Chya-Yan Liaw (2017)
Bioengineered 3D platform to explore cell-ECM interactions and drug resistance of epithelial ovarian cancer cells.
D. Loessner (2010)
3D Bioprinting a Cell-Laden Bone Matrix for Breast Cancer Metastasis Study.
X. Zhou (2016)
In vitro models for liver toxicity testing.
Valerie Y. Soldatow (2013)
Engineering liver tissue spheroids with inverted colloidal crystal scaffolds.
J. Lee (2009)
Biomimetic electrospun nanofibrous structures for tissue engineering.
X. Wang (2013)
A three-dimensional spheroidal cancer model based on PEG-fibrinogen hydrogel microspheres.
Shantanu Pradhan (2017)
The ethics of animal research
Simon Festing (2007)
Cancer is a Preventable Disease that Requires Major Lifestyle Changes
P. Anand (2008)
A reductionist metastasis‐on‐a‐chip platform for in vitro tumor progression modeling and drug screening
A. Skardal (2016)
Matrix Elasticity of Void-Forming Hydrogels Controls Transplanted Stem Cell-Mediated Bone Formation
Nathaniel Huebsch (2015)
The liver as an immunological organ
V. Racanelli (2006)
Thermoreversible hydrogel for in situ generation and release of HepG2 spheroids.
Dongdong Wang (2011)
Efficient Hepatic Delivery of Drugs: Novel Strategies and Their Significance
Nidhi Mishra (2013)
In situ expansion of engineered human liver tissue in a mouse model of chronic liver disease
K. R. Stevens (2017)
Formation of rat hepatocyte spheroids on agarose.
Y. Hasebe (2005)
Stem cell response to spatially and temporally displayed and reversible surface topography.
M. Guvendiren (2013)
Effect of matrix metalloproteinase-mediated matrix degradation on glioblastoma cell behavior in 3D PEG-based hydrogels.
Christine Wang (2017)
Exploring and Engineering the Cell Surface Interface
M. Stevens (2005)
Self‐assembly of peptide amphiphiles: From molecules to nanostructures to biomaterials
H. Cui (2010)
Layered long-term co-culture of hepatocytes and endothelial cells on a transwell membrane: toward engineering the liver sinusoid.
Young Bok Abraham Kang (2013)
Engineering tumors with 3D scaffolds
C. Fischbach (2007)
An evaluation of marginal fit of three-unit fixed dental prostheses fabricated by direct metal laser sintering system.
K. Kim (2013)
Fibronectin-matrix sandwich-like microenvironments to manipulate cell fate.
José Ballester-Beltrán (2014)
Matrigel: A complex protein mixture required for optimal growth of cell culture
C. Hughes (2010)
Development of an in vitro 3D tumor model to study therapeutic efficiency of an anticancer drug.
C. Shin (2013)
Long‐Term in Vitro Function of Adult Hepatocytes in a Collagen Sandwich Configuration
J. Dunn (1991)
Natural Materials in Tissue Engineering Applications
Elyssa L. Monzack (2011)
Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet.
Q. Chen (2016)
Editorial: Special Issue on 3D Printing of Biomaterials.
J. Miller (2016)
The epithelial polarity program: machineries involved and their hijacking by cancer
B. Tanos (2008)
Decellularized matrices as in vitro models of extracellular matrix in tumor tissues at different malignant levels: Mechanism of 5-fluorouracil resistance in colorectal tumor cells.
T. Hoshiba (2016)
3D bioprinting: improving in vitro models of metastasis with heterogeneous tumor microenvironments
Jacob L Albritton (2017)
Hydrogels with differential and patterned mechanics to study stiffness-mediated myofibroblastic differentiation of hepatic stellate cells.
M. Guvendiren (2014)
Engineering hepatocellular morphogenesis and function via ligand-presenting hydrogels with graded mechanical compliance.
E. J. Semler (2005)
Hydrogels as extracellular matrix mimics for 3D cell culture.
Mark W. Tibbitt (2009)
One step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids.
Christian Siltanen (2017)
Three-dimensional primary hepatocyte culture in synthetic self-assembling peptide hydrogel.
Sihong Wang (2008)
Hydrogels of halogenated Fmoc-short peptides for potential application in tissue engineering.
Yuqin Wang (2013)
Spheroid-based three-dimensional liver-on-a-chip to investigate hepatocyte-hepatic stellate cell interactions and flow effects.
Shi-Young Lee (2013)
Chitosan-Alginate Scaffold Culture System for Hepatocellular Carcinoma Increases Malignancy and Drug Resistance
M. Leung (2010)
Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering.
C. M. Hwang (2010)
Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants.
V. Goral (2010)
Perfused Three-dimensional Organotypic Culture of Human Cancer Cells for Therapeutic Evaluation
X. Wan (2017)
Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue
Batzaya Byambaa (2017)
Roles of spheroid formation of hepatocytes in liver tissue engineering.
Hu-Lin Jiang (2010)
Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement
C. Fischbach (2009)
Matrigel: from discovery and ECM mimicry to assays and models for cancer research.
G. Benton (2014)
Engineered 3D Cardiac Fibrotic Tissue to Study Fibrotic Remodeling
A. Sadeghi (2017)
Matrigel: basement membrane matrix with biological activity.
H. Kleinman (2005)
Microscopic anatomy of the liver
M. Krishna (2013)
The Extracellular Matrix: Not Just Pretty Fibrils
R. Hynes (2009)
Moving from static to dynamic complexity in hydrogel design.
J. Burdick (2012)
Transforming growth factor‐β and substrate stiffness regulate portal fibroblast activation in culture
Zhaodong Li (2007)
Decellularized extracellular matrix derived from porcine adipose tissue as a xenogeneic biomaterial for tissue engineering.
Young Chan Choi (2012)
Myocardial tissue engineering: in vitro models.
Gordana Vunjak Novakovic (2014)
Bioengineered 3D brain tumor model to elucidate the effects of matrix stiffness on glioblastoma cell behavior using PEG-based hydrogels.
C. Wang (2014)
Matrix Metalloproteinases: Regulators of the Tumor Microenvironment
K. Kessenbrock (2010)
Cell sources for in vitro human liver cell culture models
K. Zeilinger (2016)
A micro cell culture analog (microCCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism-dependent cytotoxicity of anti-cancer drugs.
J. Sung (2009)
Engineered in vitro disease models.
K. Benam (2015)
Organ engineering based on decellularized matrix scaffolds.
Jeremy J Song (2011)
Heparin hydrogel sandwich cultures of primary hepatocytes
E. Foster (2015)
Three-dimensional printing of Hela cells for cervical tumor model in vitro.
Yu Zhao (2014)
Hyaluronan: from extracellular glue to pericellular cue
B. Toole (2004)
Bioengineering three-dimensional culture model of human lung cancer cells: an improved tool for screening EGFR targeted inhibitors
D. Wang (2016)
The extracellular matrix in development and morphogenesis: a dynamic view.
Tania Rozario (2010)
Current Concepts of Mechanisms in Drug-Induced Hepatotoxicity
S. Russmann (2009)
Natural polymers for the microencapsulation of cells
L. Gasperini (2014)
Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro
D. Nguyen (2016)
3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs.
David B. Kolesky (2014)
Animal models of heart failure: a scientific statement from the American Heart Association.
S. Houser (2012)
Engineered Polymeric Hydrogels for 3D Tissue Models
S. Park (2016)
Biomimetic Hydrogels Incorporating Polymeric Cell-Adhesive Peptide To Promote the 3D Assembly of Tumoroids.
Y. Hao (2016)
Cell-cell interactions are essential for maintenance of hepatocyte function in collagen gel but not on matrigel.
P. Moghe (1997)
Tissue specific synthetic ECM hydrogels for 3-D in vitro maintenance of hepatocyte function.
A. Skardal (2012)
Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
April M Kloxin (2009)
Clinical development success rates for investigational drugs
M. Hay (2014)
The design of in vitro liver sinusoid mimics using chitosan-hyaluronic acid polyelectrolyte multilayers.
Y. Kim (2010)
In vitro reversion of activated primary human hepatic stellate cells
A. El Taghdouini (2015)
General review on in vitro hepatocyte models and their applications.
C. Guguen-Guillouzo (2010)
Capillary force seeding of sphere-templated hydrogels for tissue-engineered prostate cancer xenografts.
Thomas J. Long (2013)
Characterizing the effects of heparin gel stiffness on function of primary hepatocytes.
J. You (2013)
Growth Factors, Matrices, and Forces Combine and Control Stem Cells
D. Discher (2009)
Hydrolytically degradable hyaluronic acid hydrogels with controlled temporal structures.
S. Sahoo (2008)
Biomimetic macroporous hydrogel scaffolds in a high‐throughput screening format for cell‐based assays
Maria B. Dainiak (2008)
Hydrogels that mimic developmentally relevant matrix and N-cadherin interactions enhance MSC chondrogenesis
L. Bian (2013)
Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures.
Liora Almany (2005)
The toxin biliatresone causes mouse extrahepatic cholangiocyte damage and fibrosis through decreased glutathione and SOX17
Orith Waisbourd-Zinman (2016)
Hydrogel embedding of precision‐cut liver slices in a microfluidic device improves drug metabolic activity
P. V. van Midwoud (2011)
Novel hepatocyte culture system developed using microfabrication and collagen/polyethylene glycol microcontact printing.
J. Fukuda (2006)
Structure and function of hepatic stellate cells
H. Senoo (2003)
Capturing complex 3D tissue physiology in vitro
L. Griffith (2006)
Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs
S. Ji (2017)
Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation
J. Jeon (2014)
Cell polarity in development and cancer
A. Wodarz (2007)
Development of an Acellular Tumor Extracellular Matrix as a Three-Dimensional Scaffold for Tumor Engineering
Wei-Dong Lü (2014)
Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion
S. Peyton (2005)
Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions.
Masumi Yamada (2012)
Tubeless microfluidic angiogenesis assay with three-dimensional endothelial-lined microvessels.
Lauren L Bischel (2013)
Persistence and activation of malaria hypnozoites in long-term primary hepatocyte cultures
Laurent Dembélé (2014)
Preparation and characterization of chitosan/galactosylated hyaluronic acid scaffolds for primary hepatocytes culture
Jinyong Fan (2010)
Tumor Bioengineering Using a Transglutaminase Crosslinked Hydrogel
J. Fang (2014)
Electrospinning: a fascinating fiber fabrication technique.
Nandana Bhardwaj (2010)
3D Printed Tissue Models: Present and Future.
J. Jang (2016)
Alginate based 3D hydrogels as an in vitro co-culture model platform for the toxicity screening of new chemical entities.
Shih-Feng Lan (2011)
Stiffening hydrogels for investigating the dynamics of hepatic stellate cell mechanotransduction during myofibroblast activation
S. Caliari (2016)
Morphological and Functional Analysis of Hepatocyte Spheroids Generated on Poly-HEMA-Treated Surfaces under the Influence of Fetal Calf Serum and Nonparenchymal Cells
A. Acikgoez (2013)
In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration
Chenxia Hu (2015)
Multicellular tumor spheroids: an underestimated tool is catching up again.
F. Hirschhaeuser (2010)
Controlling stem cell fate with material design.
Ross Marklein (2010)
Decellularized matrices for tissue engineering
T. Hoshiba (2010)
Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture.
M. Bhattacharya (2012)
Hydrogel network design using multifunctional macromers to coordinate tissue maturation in ovarian follicle culture.
A. Shikanov (2011)
Immunological hallmarks of stromal cells in the tumour microenvironment
S. Turley (2015)
Regulation of proteoglycan expression in fibrotic liver and cultured fat-storing cells.
A. M. Gressner (1994)
Biomimetic 4D printing.
A. S. Gladman (2016)
A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
Hyun-wook Kang (2016)
Biomimetic nanofibrous scaffolds for bone tissue engineering.
Jeremy M. Holzwarth (2011)
Colloidal crystals as templates for porous materials
O. Velev (2000)
Biofunctionalized Hydrogel Microscaffolds Promote 3D Hepatic Sheet Morphology.
M. Kim (2016)
Gradually softening hydrogels for modeling hepatic stellate cell behavior during fibrosis regression.
S. Caliari (2016)
Three-dimensional microfluidic model for tumor cell intravasation and endothelial barrier function
I. Zervantonakis (2012)
Are animal models predictive for humans?
N. Shanks (2009)
Interpenetrating Polymer Networks polysaccharide hydrogels for drug delivery and tissue engineering.
P. Matricardi (2013)
Decellularization of tissues and organs.
T. W. Gilbert (2006)
Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform
A. Skardal (2017)
Cross talk between cancer and immune cells: exploring complex dynamics in a microfluidic environment.
L. Businaro (2013)
Microengineered Liver Tissues for Drug Testing
S. Khetani (2015)
Structure of the extracellular matrix in normal and fibrotic liver: collagens and glycoproteins.
D. Schuppan (1990)
Recreating the tumor microenvironment in a bilayer, hyaluronic acid hydrogel construct for the growth of prostate cancer spheroids.
Xian Xu (2012)
Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays.
C. M. Williams (2011)
Microfluidic 3D cell culture: from tools to tissue models.
V. van Duinen (2015)
Alginate: A Versatile Biomaterial to Encapsulate Isolated Ovarian Follicles
J. Vanacker (2017)
Bioprinting the Cancer Microenvironment.
Y. Zhang (2016)
Engineering Photocrosslinkable Bicomponent Hydrogel Constructs for Creating 3D Vascularized Bone
M. Kazemzadeh-Narbat (2017)
An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture.
P. J. Lee (2007)
Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting
Xuanyi Ma (2016)
Decellularized extracellular matrix: a step towards the next generation source for bioink manufacturing.
B. Kim (2017)
Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels
Sudhir Khetan (2013)
Microenvironment complexity and matrix stiffness regulate breast cancer cell activity in a 3D in vitro model
Marta Cavo (2016)
Micropatterned cell-cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues.
C. Li (2014)
Cell-laden hydrogels for osteochondral and cartilage tissue engineering.
Jingzhou Yang (2017)
Cancer: Nanoscience and Nanotechnology Approaches
W. Chan (2017)
Generation of human-induced pluripotent stem cells
In-Hyun Park (2008)
Designing Biomaterials for 3D Printing.
M. Guvendiren (2016)
Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (MSCs).
F. P. Seib (2009)
Quantification of Extracellular Matrix Proteins from a Rat Lung Scaffold to Provide a Molecular Readout for Tissue Engineering*
Ryan C. Hill (2015)
Characterization of the secreted proteome of rat hepatocytes cultured in collagen sandwiches.
D. Farkas (2005)
A 3D model of ovarian cancer cell lines on peptide nanofiber scaffold to explore the cell–scaffold interaction and chemotherapeutic resistance of anticancer drugs
Zehong Yang (2011)
Materials from peptide assembly: towards the treatment of cancer and transmittable disease
M. Branco (2011)
Engineering synthetic hydrogel microenvironments to instruct stem cells.
M. Guvendiren (2013)
An overview of tissue and whole organ decellularization processes.
Peter M. Crapo (2011)
Polysaccharide biomaterials for drug delivery and regenerative engineering
N. Shelke (2014)
The independent roles of mechanical, structural and adhesion characteristics of 3D hydrogels on the regulation of cancer invasion and dissemination.
Jennifer N. Beck (2013)
Diagnosis of cirrhosis by transient elastography (FibroScan): a prospective study
J. Foucher (2006)
Biofabrication of a three-dimensional liver micro-organ as an in vitro drug metabolism model.
Robert C. Chang (2010)
Role of Kupffer cells in the pathogenesis of liver disease.
G. Kolios (2006)
Hallmarks of Cancer: The Next Generation
D. Hanahan (2011)
Lost in translation: animal models and clinical trials in cancer treatment.
Isabella W Y Mak (2014)
3D Cultivation Techniques for Primary Human Hepatocytes
A. Bachmann (2015)
Harnessing Traction-Mediated Manipulation of the Cell-Matrix Interface to Control Stem Cell Fate
Nathaniel Huebsch (2010)
Modeling mass transfer in hepatocyte spheroids via cell viability, spheroid size, and hepatocellular functions
R. Glicklis (2004)
The role of matrix stiffness in hepatic stellate cell activation and liver fibrosis.
R. G. Wells (2005)
A three-dimensional in vitro tumor platform for modeling therapeutic irreversible electroporation.
C. Arena (2012)
Chitosan-alginate 3D scaffolds as a mimic of the glioma tumor microenvironment.
Forrest M Kievit (2010)
A microfluidic 3D in vitro model for specificity of breast cancer metastasis to bone.
S. Bersini (2014)
Hepatic fibrosis in children and adults
R. Wells (2017)
Quantification of decellularized human myocardial matrix: A comparison of six patients
T. D. Johnson (2016)
Multilayered heparin hydrogel microwells for cultivation of primary hepatocytes.
J. You (2014)
3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments.
A. Taubenberger (2016)
Biofabrication of 3D Alginate-Based Hydrogel for Cancer Research: Comparison of Cell Spreading, Viability, and Adhesion Characteristics of Colorectal HCT116 Tumor Cells.
Jelena Ivanovska (2016)
Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels.
T. Sellaro (2010)
Heparin-based hydrogel as a matrix for encapsulation and cultivation of primary hepatocytes.
Mihye Kim (2010)
Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules
Yue Xu (2010)
Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments.
N. Koide (1990)
Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels.
S. Bryant (2002)
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
D. Discher (2005)
Energy metabolism in the liver.
L. Rui (2014)
Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels
Yoojin Shin (2012)
Chitosan-Based Thermoreversible Hydrogel as an in Vitro Tumor Microenvironment for Testing Breast Cancer Therapies
Ching-Ting Tsao (2014)
This paper is referenced by
Bio-Inspired Hydrogels via 3D Bioprinting
Lei Nie (2020)
Exploring the Role of Nanoparticles in Enhancing Mechanical Properties of Hydrogel Nanocomposites
J. Zaragoza (2018)
The contributions of mesoderm-derived cells in liver development.
L. Yang (2018)
Bioprinting Perfusion-Enabled Liver Equivalents for Advanced Organ-on-a-Chip Applications
T. Grix (2018)
Selected Papers from the 1st International Online Conference on Nanomaterials
A. Díez-Pascual (2019)
Could 3D models of cancer enhance drug screening?
V. Brancato (2019)
Principles of Tissue Engineering
A. X. Chen (2020)
Low-Molecular-Weight Heparin-Functionalized Chitosan-Chondroitin Sulfate Hydrogels for Controlled Release of TGF-β3 and in vitro Neocartilage Formation
Y. Chen (2019)
Multiphasic microgel-in-gel materials to recapitulate cellular mesoenvironments in vitro.
Dejan Husman (2019)
Hydrophobically-modified gelatin hydrogel as a carrier for charged hydrophilic drugs and hydrophobic drugs.
T. Takei (2020)
A fully degradable and photocrosslinked polysaccharide-polyphosphate hydrogel for tissue engineering.
Y. Hao (2019)
Development and characterization of a low-cost 3D bioprinter
Bekir Yenilmez (2019)
Novel bioinks from UV-responsive norbornene-functionalized carboxymethyl cellulose macromers
S. Ji (2020)
Advances in Hydrogels in Organoids and Organs-on-a-Chip.
Hongzhen Liu (2019)
Design of spherically structured 3D in vitro tumor models -Advances and prospects.
L. Ferreira (2018)
Developments with 3D bioprinting for novel drug discovery
A. Satpathy (2018)
Hollow Alginate-Poly-L-Lysine-Alginate Microspheres Promoted an Epithelial-Mesenchymal Transition in Human Colon Adenocarcinoma Cells
Shirin Saberianpour (2020)
An integrated biomimetic array chip for high-throughput co-culture of liver and tumor microtissues for advanced anticancer bioactivity screening.
Y. Hou (2020)
Fabrication of Artificial Nanobasement Membranes for Cell Compartmentalization in 3D Tissues.
Jinfeng Zeng (2020)
Three dimensional engineered models to study hypoxia biology in breast cancer.
Vaishali Aggarwal (2020)
Hybrid 3D Printing of Synthetic and Cell-Laden Bioinks for Shape Retaining Soft Tissue Grafts.
Sarah Van Belleghem (2020)
Microfluidics‐Assisted Fabrication of Microtissues with Tunable Physical Properties for Developing an In Vitro Multiplex Tissue Model
Dongjin Lee (2018)
Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine.
Feihu Xie (2020)
Hydrogel-Based Localized Nonviral Gene Delivery in Regenerative Medicine Approaches—An Overview
Natalia Carballo-Pedrares (2020)
Manipulating the Deformation of Swelling Hydrogel Models by Microparticles
T. Ma (2020)
Decellularized Tissues as Platforms for In Vitro Modeling of Healthy and Diseased Tissues.
Michaela W McCrary (2020)
The Construction and Application of Three‐Dimensional Biomaterials
J. Wang (2020)
Biomedical applications of nanofibrillar cellulose
P. Laurén (2018)
Synthetic Polymers Provide a Robust Substrate for Functional Neuron Culture.
Y. Zhang (2020)
Anticancer drug discovery using multicellular tumor spheroid models
Michele Zanoni (2019)
3D bioprinting of complex channels within cell-laden hydrogels.
S. Ji (2019)
Practical Applications of 3D Cell Culture Biotechnologies for Translational Oncology and Personalized Therapy
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