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Potential Of Different Enzyme Immobilization Strategies To Improve Enzyme Performance

Cristina Garcia-Galan, A. Berenguer-Murcia, R. Fernández-Lafuente, Rafael C. Rodrigues
Published 2011 · Chemistry

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Enzyme biocatalysis plays a very relevant role in the development of many chemical industries, e.g., energy, food or fine chemistry. To achieve this goal, enzyme immobilization is a usual pre-requisite as a solution to get reusable biocatalysts and thus decrease the price of this relatively expensive compound. However, a proper immobilization technique may permit far more than to get a reusable enzyme; it may be used to improve enzyme performance by improving some enzyme limitations: enzyme purity, stability (including the possibility of enzyme reactivation), activity, specificity, selectivity, or inhibitions. Among the diverse immobilization techniques, the use of pre-existing supports to immobilize enzymes (via covalent or physical coupling) and the immobilization without supports [enzyme crosslinked aggregates (CLEAs) or crystals (CLECs)] are the most used or promising ones. This paper intends to give the advantages and disadvantages of the different existing immobilization strategies to solve the different aforementioned enzyme limitations. Moreover, the use of nanoparticles as immobilization supports is achieving an increasing importance, as the nanoparticles versatility increases and becomes more accessible to the researchers. We will also discuss here some of the advantages and drawbacks of these non porous supports compared to conventional porous supports. Although there are no universal optimal solutions for all cases, we will try to give some advice to select the optimal strategy for each particular enzyme and process, considering the enzyme properties, nature of the process and of the substrate. In some occasions the selection will be compulsory, for example due to the nature of the substrate. In other cases the optimal biocatalyst may depend on the company requirements (e.g., volumetric activity, enzyme stability, etc).
This paper references
10.1021/OP0600308
Chemocatalysis and Biocatalysis (Biotransformation): Some Thoughts of a Chemist and of a Biotechnologist
J. Leresche (2006)
10.1002/BIT.10303
Advantages of using non-isothermal bioreactors for the enzymatic synthesis of antibiotics: the penicillin G acylase as enzyme model.
P. Travascio (2002)
10.1016/S1093-0191(00)00078-2
Use of chemically modified chitosan beads for sorption and enzyme immobilization
R. Juang (2002)
10.1016/S0032-9592(01)00207-2
Purification of large proteins using ion-exchange membranes
C. S. Rao (2001)
10.1016/j.cbpa.2009.11.023
Multi-enzymatic synthesis.
Fernando López-Gallego (2010)
10.1016/J.PROCBIO.2009.02.001
Reactivation of covalently immobilized lipase from Thermomyces lanuginosus
R. Rodrigues (2009)
10.1002/bit.260390613
Determination of total and active immobilized enzyme distribution in porous solid supports
R. Scharer (1992)
10.1016/S0141-0229(98)00053-2
The presence of methanol exerts a strong and complex modulation of the synthesis of different antibiotics by immobilized penicillin G acylase
R. Fernández-Lafuente (1998)
10.1021/bp025588n
Hydrolysis of Proteins by Immobilized‐Stabilized Alcalase‐Glyoxyl Agarose
P. W. Tardioli (2003)
10.1021/JA002261E
Synthesis of New, Nanoporous Carbon with Hexagonally Ordered Mesostructure
S. Jun (2000)
10.1016/S0141-0229(98)00084-2
Comparison of techniques for enzyme immobilization on silicon supports
A. Subramanian (1999)
10.2174/138527210792927591
Enzymatic Promiscuity for Organic Synthesis and Cascade Process
Qi Wu (2010)
10.1271/bbb1961.53.2263
The Co-immobilization of Alcohol Dehydrogenase, Diaphorase, and NAD on Glutaraldehydeactivated Sepharose
H. Ukeda (1989)
10.1021/CM020784A
Direct Assembly of Gold Nanoparticle “Shells” on Polyurethane Microsphere “Cores” and Their Application as Enzyme Immobilization Templates
S. Phadtare (2003)
10.1016/S0378-4347(98)00053-X
Characterization and affinity applications of cellulose-binding domains.
P. Tomme (1998)
10.1016/S1381-1177(99)00028-4
Stabilization of multimeric enzymes via immobilization and post-immobilization techniques
R. Fernández-Lafuente (1999)
10.1271/bbb1961.46.1571
The Co-immobilization of NAD and Dehydrogenases and Its Application to Bioreactors for Synthesis and Analysis
Y. Yamazaki (1982)
10.1016/J.MOLCATB.2004.11.003
Dextran aldehyde coating of glucose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles
L. Betancor (2005)
10.1002/ELPS.200500587
Functionalized magnetic micro‐ and nanoparticles: Optimization and application to μ‐chip tryptic digestion
Z. Bı́lková (2006)
10.1021/BM034528I
Cross-linked aggregates of multimeric enzymes: a simple and efficient methodology to stabilize their quaternary structure.
Lorena Wilson (2004)
10.1016/J.ENZMICTEC.2006.08.023
Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability
J. Pedroche (2007)
10.1021/AC00067A021
Engineering protein orientation at surfaces to control macromolecular recognition events.
M. A. Mclean (1993)
10.1002/BIT.10019
One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports.
C. Mateo (2001)
10.1038/nchembio.203
Directed evolution drives the next generation of biocatalysts.
N. Turner (2009)
10.1016/S0304-8853(99)00005-0
Immobilization of proteins and enzymes to fine magnetic particles
M. Koneracká (1999)
10.1002/(SICI)1097-0290(19980605)58:5<486::AID-BIT4>3.0.CO;2-9
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
Bastida (1998)
10.1016/0142-9612(95)99638-3
Covalent immobilization of α-amylase onto pHEMA microspheres: preparation and application to fixed bed reactor
M. Y. Arica (1995)
10.1007/s11244-009-9279-0
Forty Years of Designing Catalytic and Adsorptive Sites in FAU Type Zeolites at K.U. Leuven
Johan A Martens (2009)
10.1002/ADSC.201100421
Enabling Industrial Biocatalytic Processes by Application of Silicone-Coated Enzyme Preparations
R. Nieguth (2011)
10.1007/S00449-007-0176-2
Influence of mass transfer limitations on the enzymatic synthesis of β-lactam antibiotics catalyzed by penicillin G acylase immobilized on glioxil-agarose
L. R. B. Gonçalves (2008)
10.1002/BIT.260400614
Determination of intraparticle immobilized enzyme distribution under moderate diffusion conditions.
M. M. Hossain (1992)
10.1002/BIT.20131
Enabling multienzyme biocatalysis using nanoporous materials
B. El-Zahab (2004)
10.1016/J.TETASY.2006.01.020
Synthesis of enantiomerically pure (S)-mandelic acid using an oxynitrilase–nitrilase bienzymatic cascade: a nitrilase surprisingly shows nitrile hydratase activity
C. Mateo (2006)
10.1186/1472-6750-7-54
New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase
D. Cecchini (2007)
10.1021/ja00540a026
Enzyme immobilization by condensation copolymerization into crosslinked polyacrylamide gels
A. Pollak (1980)
10.1021/BM025512F
Coimmobilization of gluconolactonase with glucose oxidase for improvement in kinetic property of enzymatically induced volume collapse in ionic gels.
K. Ogawa (2002)
10.1042/BST0351583
Cross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts.
R. Sheldon (2007)
10.1016/J.ENZMICTEC.2005.03.020
Some special features of glyoxyl supports to immobilize proteins
C. Mateo (2005)
10.1016/j.enzmictec.2011.03.002
Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene-divinylbenzene beads.
K. Hernández (2011)
10.1002/BIT.10781
Catalytic behaviors of enzymes attached to nanoparticles: the effect of particle mobility
H. Jia (2003)
10.1038/35051736
Industrial biocatalysis today and tomorrow
A. Schmid (2001)
10.1002/cctc.200900199
Composite Particles of Novozyme 435 and Silicone: Advancing Technical Applicability of Macroporous Enzyme Carriers
L. O. Wiemann (2009)
10.1007/3-540-49811-7_6
Bioaffinity based immobilization of enzymes.
M. Saleemuddin (1999)
10.1007/BF00256663
Use of magnetic particles isolated from magnetotactic bacteria for enzyme immobilization
T. Matsunaga (2004)
10.1016/0003-2670(94)80234-3
Reactivation of an immobilized enzyme reactor for the determination of acetylcholinesterase inhibitors Flow injection determination of paraoxon
C. G. D. María (1994)
10.1016/0167-7799(93)90080-S
Immobilized enzymes--learning from past successes and failures.
E. Katchalski-Katzir (1993)
10.1002/bit.22198
Utilization of cross‐linked laccase aggregates in a perfusion basket reactor for the continuous elimination of endocrine‐disrupting chemicals
H. Cabana (2009)
10.1021/IE010585L
Purification of a large protein using ion-exchange membranes
H. Yang (2002)
10.2174/092986709788803141
Biomedical applications of protein microarrays.
S. Spisák (2009)
10.1002/1097-0290(20000220)72:4<458::AID-BIT1007>3.0.CO;2-R
Diffusion and chemical reaction rates with nonuniform enzyme distribution: an experimental approach.
M. Ladero (2001)
10.1002/(SICI)1097-0290(19960720)51:2<221::AID-BIT12>3.0.CO;2-P
Affinity precipitation and site‐specific immobilization of proteins carrying polyhistidine tails
J. Carlsson (1996)
10.1002/ADSC.200900303
Cross‐Linked Enzyme Aggregates of Chloroperoxidase: Synthesis, Optimization and Characterization
D. I. Perez (2009)
10.1021/BM050088E
Co-aggregation of enzymes and polyethyleneimine: a simple method to prepare stable and immobilized derivatives of glutaryl acylase.
F. López-Gallego (2005)
10.1016/S1381-1177(02)00178-9
Interfacial adsorption of lipases on very hydrophobic support (octadecyl-Sepabeads): Immobilization, hyperactivation and stabilization of the open form of lipases
J. Palomo (2002)
10.1166/JNN.2007.869
Design of superparamagnetic iron oxide nanoparticle for purification of recombinant proteins.
S. Mohapatra (2007)
10.1016/S0009-2509(01)00032-X
Optimization of fed-batch bioreactor for immobilized enzyme processes
S. Sengupta (2001)
10.1002/BIT.21584
Particle‐tethered NADH for production of methanol from CO2 catalyzed by coimmobilized enzymes
B. El-Zahab (2008)
10.1016/J.JBIOTEC.2005.05.021
Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports.
F. López-Gallego (2005)
10.1016/S1381-1177(00)00011-4
Preparation of new lipases derivatives with high activity-stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization with polyethylenimine
J. M. Guisan (2001)
10.1002/(SICI)1097-0290(19960305)49:5<527::AID-BIT5>3.0.CO;2-L
Efficient immobilization of lipases by entrapment in hydrophobic sol‐gel materials
M. Reetz (1996)
10.1021/PR060558R
Microchip reactor packed with metal-ion chelated magnetic silica microspheres for highly efficient proteolysis.
Y. Li (2007)
10.1002/bit.260310716
Determination of intrinsic parameters for immobilization reactions of catalase and amyloglucosidase in porous glass supports
M. M. Hossain (1988)
10.1385/ABAB:106:1-3:413
Hybrid model for an enzymatic reactor
R. Sousa (2003)
10.1021/bp0342102
New Cationic Exchanger Support for Reversible Immobilization of Proteins
M. Fuentes (2004)
10.1002/bit.260340810
Properties of a reversible soluble–insoluble cellulase and its application to repeated hydrolysis of crystalline cellulose
Machiko Taniguchi (1989)
10.1016/0076-6879(88)37055-2
[53] Enzyme stabilization by immobilization
P. Monsan (1988)
10.1126/SCIENCE.1079237
Dispelling the Myths--Biocatalysis in Industrial Synthesis
H. Schoemaker (2003)
10.1042/BST0351593
Advances in the design of new epoxy supports for enzyme immobilization-stabilization.
C. Mateo (2007)
10.1002/(SICI)1097-0290(19960320)49:6<654::AID-BIT6>3.0.CO;2-N
Catalytic durability of magnetoproteoliposomes captured by high‐gradient magnetic forces in a miniature fixed‐bed reactor
M. de Cuyper (1996)
10.1002/bit.260350706
Solvent selection for biocatalysis in mainly organic systems: Predictions of effects on equilibrium position
P. Halling (1990)
10.1016/S0165-022X(01)00206-8
Twenty-five years of immobilized metal ion affinity chromatography: past, present and future.
G. Chaga (2001)
10.1016/j.jbiotec.2009.09.002
Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers.
Rafael C. Rodrigues (2009)
10.1021/AC034920M
Magnetite-containing spherical silica nanoparticles for biocatalysis and bioseparations.
H. Yang (2004)
10.1016/J.ENZMICTEC.2006.01.024
Purification and very strong reversible immobilization of large proteins on anionic exchangers by controlling the support and the immobilization conditions
B. Pessela (2006)
10.1016/S1381-1177(02)00224-2
Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins: Hydrolytic resolution of (R,S)-2-butyroyl-2-phenylacetic acid
J. Palomo (2003)
10.1016/J.JSSC.2010.08.027
Mesoporous mixed metal oxides derived from P123-templated Mg–Al layered double hydroxides
Jun Wang (2010)
10.1039/b815716b
Capillary microreactors wall-coated with mesoporous titania thin film catalyst supports.
Evgeny V. Rebrov (2009)
10.1016/J.BEJ.2005.03.003
On the effectiveness factor calculation for a reaction - diffusion process in an immobilized biocatalyst pellet
J. C. Gottifredi (2005)
10.1016/S0141-0229(98)00028-3
The coimmobilization of d-amino acid oxidase and catalase enables the quantitative transformation of d-amino acids (d-phenylalanine) into α-keto acids (phenylpyruvic acid)
R. Fernández-Lafuente (1998)
10.1002/elsc.200900061
Bioethanol and biodiesel: Alternative liquid fuels for future generations
G. Sivakumar (2010)
10.1016/J.ENZMICTEC.2009.02.009
Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipase from Thermomyces lanuginosus
R. Rodrigues (2009)
10.3109/10242420108992032
Stabilization of Immobilized Enzymes Against Water-Soluble Organic Cosolvents and Generation of Hyper-Hydrophilic Micro-Environments Surrounding Enzyme Molecules
O. Abián (2001)
10.1016/J.PROCBIO.2010.07.001
Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports
J. M. Bolivar (2010)
10.1038/359710A0
Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism
C. Kresge (1992)
10.1016/0022-2836(92)90225-9
The crystal and molecular structure of the Rhizomucor miehei triacylglyceride lipase at 1.9 A resolution.
Z. Derewenda (1992)
10.1016/S1381-1177(00)00041-2
Interfacial catalysis by lipases
N. Miled (2001)
10.1016/0020-711X(87)90073-5
Some observations on the inhibition and activation of a thermophilic protease.
D. Cowan (1987)
10.1006/ABIO.2001.5214
A novel purification method for histidine-tagged proteins containing a thrombin cleavage site.
M. Hefti (2001)
10.1038/nprot.2007.209
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins
Thomas G. M. Schmidt (2007)
10.1021/OL005593X
Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase.
L. Cao (2000)
10.1016/J.CHROMA.2004.01.061
Ion exchange using poorly activated supports, an easy way for purification of large proteins.
B. Pessela (2004)
10.1016/J.MOLCATB.2010.09.015
Reactivation of immobilized penicillin G acylase: Influence of cosolvents and catalytic modulators
V. Miranda (2011)
10.1016/J.PROCBIO.2010.12.007
Lipase B from Candida antarctica immobilized on octadecyl Sepabeads: A very stable biocatalyst in the presence of hydrogen peroxide
K. Hernández (2011)
10.1021/bp0340638
Enzyme Distribution Derived from Macroscopic Particle Behavior of an Industrial Immobilized Penicillin‐G Acylase
Jeroen L. Van Roon (2003)
10.1016/0141-0229(94)90043-4
Thermodynamic predictions for biocatalysis in nonconventional media: theory, tests, and recommendations for experimental design and analysis.
P. Halling (1994)
10.1016/J.MOLCATB.2006.06.011
Enantioselectivity modulation through immobilization of Arthrobacter sp. lipase: Kinetic resolution of fluoxetine intermediate
A. Chaubey (2006)
10.1002/SMLL.200500035
Simple synthesis of hierarchically ordered mesocellular mesoporous silica materials hosting crosslinked enzyme aggregates.
J. Lee (2005)
10.1002/EJLT.200800100
The evolution of enzymatic interesterification in the oils and fats industry
H. C. Holm (2008)
10.1007/978-1-4612-0057-4_34
Hybrid Model for an Enzymatic Reactor
R. Sousa (2003)
10.2210/pdb4tgl/pdb
Catalysis at the interface: the anatomy of a conformational change in a triglyceride lipase.
U. Derewenda (1992)
10.1002/(SICI)1097-0290(19981120)60:4<518::AID-BIT14>3.0.CO;2-D
Use of dextrans as long and hydrophilic spacer arms to improve the performance of immobilized proteins acting on macromolecules.
G. Penzol (1998)
10.1016/J.MEMSCI.2004.04.002
Immobilization of cross-linked lipase aggregates within microporous polymeric membranes
N. Hilal (2004)
10.1016/j.tibtech.2008.06.009
Bioinspired enzyme encapsulation for biocatalysis.
Lorena Betancor (2008)
10.1016/J.CHROMA.2004.03.058
Use of immobilized lipases for lipase purification via specific lipase-lipase interactions.
J. Palomo (2004)
10.1016/j.enzmictec.2011.02.005
Hydrolysis of triacetin catalyzed by immobilized lipases: effect of the immobilization protocol and experimental conditions on diacetin yield.
K. Hernández (2011)
10.1111/J.1432-1033.1997.00220.X
Regulation and properties of a fungal lipase showing interfacial inactivation by gas bubbles, or droplets of lipid or fatty acid.
K. Stahmann (1997)
10.1139/W06-094
Chemical modification results in hyperactivation and thermostabilization of Fusarium solani glucoamylase.
H. N. Bhatti (2007)
10.1016/S0040-4020(03)00876-7
Regio-selective deprotection of peracetylated sugars via lipase hydrolysis
G. Fernandez-Lorente (2003)
10.3109/10242420009015243
Production and Characterisation of Cross-Linked Enzyme Crystals (Clecs®) for Application as Process Scale Biocatalysts
J. Vaghjiani (2000)
10.1016/J.FOODCHEM.2004.07.020
Peptide generation from casein hydrolysis by immobilised porcine cathepsins
F. Toldrá (2005)
10.1016/0378-4347(87)80007-5
Chromatography of complex protein mixtures
F. Regnier (1987)
10.1016/J.CBPA.2005.02.014
Immobilised enzymes: science or art?
L. Cao (2005)
10.1021/la105051v
Synthesis of magnetic noble metal (nano)particles.
Krishna N K Kowlgi (2011)
10.1016/S1381-1177(00)00078-3
Cross-linked aggregates of penicillin acylase: robust catalysts for the synthesis of β-lactam antibiotics
L. Cao (2001)
10.1021/OP100289F
Cross-Linked Enzyme Aggregates as Industrial Biocatalysts
R. Sheldon (2011)
10.1016/J.ENZMICTEC.2007.01.018
Improvement of enzyme activity, stability and selectivity via immobilization techniques
C. Mateo (2007)
10.2174/138527210791130514
New Trends in the Recycling of NAD(P)H for the Design of Sustainable Asymmetric Reductions Catalyzed by Dehydrogenases
Angel Berenguer-Murcia and Roberto Fernandez-Lafuente (2010)
10.1016/S0003-2697(79)80110-4
Enzyme stabilization by immobilization.
A. Klibanov (1979)
10.1039/B101489G
Biochemically functionalized silica nanoparticles.
M. Qhobosheane (2001)
10.1006/JMBI.1994.1035
Crystallization and preliminary X-ray studies of lipase B from Candida antarctica.
Jonas Uppenberg (1994)
10.1016/j.biortech.2009.09.014
In situ immobilization of acid protease on mesoporous activated carbon packed column for the production of protein hydrolysates.
A. Ganesh Kumar (2010)
10.1002/biot.200900049
Application of immobilized bovine enterokinase in repetitive fusion protein cleavage for the production of mucin 1.
T. Kubitzki (2009)
10.1016/j.jbiotec.2008.09.015
Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration.
Wenfang Liu (2009)
10.1016/j.enzmictec.2010.10.003
Control of protein immobilization: coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance.
K. Hernandez (2011)
10.1002/bit.260380611
Enzymatic resolution of (S)‐(+)‐naproxen in a continuous reactor
E. Battistel (1991)
10.1002/BIT.21107
Crosslinked enzyme aggregates in hierarchically‐ordered mesoporous silica: A simple and effective method for enzyme stabilization
M. Kim (2007)
10.1016/J.ENZMICTEC.2003.12.013
Application of chitin- and chitosan-based materials for enzyme immobilizations: a review
B. Krajewska (2004)
10.1007/S11746-008-1261-7
Efficient Immobilization of Lecitase in Gelatin Hydrogel and Degumming of Rice Bran Oil Using a Spinning Basket Reactor
G. Sheelu (2008)
10.1016/0005-2744(80)90132-1
Renaturation and urea-induced denaturation of 20 beta-hydroxysteroid dehydrogenase studied in solution and in the immobilized state.
P. Pasta (1980)
10.1016/0141-0229(93)90022-T
Enhanced recovery and purification of Aspergillus glucoamylase from Saccharomyces cerevisiae by the addition of poly(aspartic acid) tails.
I. Suominen (1993)
10.1016/S0141-0229(98)00102-1
Facile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: Dramatic stabilization of penicillin acylase versus organic solvents
R. Fernández-Lafuente (1999)
10.1016/j.biortech.2011.03.018
Combined cross-linked enzyme aggregates from versatile peroxidase and glucose oxidase: production, partial characterization and application for the elimination of endocrine disruptors.
R. Taboada-Puig (2011)
10.1002/(SICI)1097-0290(20000205)67:3<319::AID-BIT8>3.0.CO;2-S
Effect of mass-transfer limitations on the selectivity of immobilized alpha-chymotrypsin biocatalysts prepared for use in organic medium.
R. J. Barros (2000)
10.1016/J.MOLCATB.2010.06.013
Enzyme-mediated domino synthesis of 2-alkylbenzimidazoles in solvent-free system: A green route to heterocyclic compound
Li Wang (2010)
10.1007/s00239-010-9415-2
Exploiting Models of Molecular Evolution to Efficiently Direct Protein Engineering
Megan F. Cole (2010)
10.1002/bit.260260208
Mathematical modeling of diffusion and reaction in the hydrolysis of vegetable protein in an immobilized enzyme recycle reactor
B. Adu-Amankwa (1984)
10.1002/j.1460-2075.1985.tb03741.x
Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.
B. Nilsson (1985)
10.1021/JA021223N
Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles.
Ansil Dyal (2003)
10.1016/J.ENZMICTEC.2006.01.014
Different mechanisms of protein immobilization on glutaraldehyde activated supports: Effect of support activation and immobilization conditions
L. Betancor (2006)
10.1016/S1381-1177(00)00124-7
Eupergit® C, a carrier for immobilization of enzymes of industrial potential
E. Katchalski-Katzir (2000)
10.1021/CM0483137
Mesoporous Silica Spheres as Supports for Enzyme Immobilization and Encapsulation
Yajun Wang (2005)
10.1016/0956-5663(91)80011-L
Determination of glucose diffusion coefficients in biofilms with micro-electrodes.
C. Cronenberg (1991)
10.1016/J.ENZMICTEC.2005.08.034
Glyoxyl agarose as a new chromatographic matrix
V. Grazú (2006)
10.1016/J.CHROMA.2004.08.141
Selective and mild adsorption of large proteins on lowly activated immobilized metal ion affinity chromatography matrices. Purification of multimeric thermophilic enzymes overexpressed in Escherichia coli.
B. Pessela (2004)
10.1016/J.MOLCATB.2011.04.008
Effect of the immobilization protocol on the properties of lipase B from Candida antarctica in organic media: Enantiospecifc production of atenolol acetate
Oveimar Barbosa (2011)
10.1016/J.ENZMICTEC.2006.04.015
Modulation of the catalytic properties of multimeric β-galactosidase from E. coli by using different immobilization protocols
B. Pessela (2007)
10.1016/J.ENZMICTEC.2004.07.019
Purification of keratinase from poultry farm isolate-Scopulariopsis brevicaulis and statistical optimization of enzyme activity
P. Anbu (2005)
10.1016/1046-5928(92)90001-D
Immobilized metal ion affinity chromatography.
J. Porath (1992)
10.1111/J.1365-2621.2010.02177.X
Enzymes in juice processing: a review
D. S. Ribeiro (2010)
10.1021/bp025785m
Preparation of a Stable Biocatalyst of Bovine Liver Catalase Using Immobilization and Postimmobilization Techniques
L. Betancor (2003)
10.1016/S0141-0229(03)00160-1
Enzyme stabilization: recent experimental progress
C. Ó'Fágáin (2003)
10.1016/J.MEMSCI.2004.10.024
Immobilization of cellulase in nanofibrous PVA membranes by electrospinning
L. Wu (2005)
10.1016/J.PROCBIO.2009.08.014
Complete reactivation of immobilized derivatives of a trimeric glutamate dehydrogenase from Thermus thermophillus
J. M. Bolivar (2010)
10.1016/J.ENZMICTEC.2009.08.009
Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation
Roberto Fernández-Lafuente (2009)
10.1007/BF02922147
Glucoamylase immobilization on a magnetic microparticle for the continuous hydrolysis of maltodextrin in a fluidized bed reactor
B. Pieters (1992)
10.1016/J.MICROMESO.2008.05.004
Grafting or tethering titanium alkoxo complexes on MCM-41? Strategies to prepare epoxidation catalysts
R. Ballesteros (2008)
10.1002/ADSC.200600337
In Silico Analysis of Enzyme Surface and Glycosylation Effect as a Tool for Efficient Covalent Immobilisation of CalB and PGA on Sepabeads
A. Basso (2007)
10.1016/J.ENZMICTEC.2006.03.020
Enzymatic activities of proteases immobilized on tri(4-formyl phenoxy) cyanurate
R. S. Rao (2006)
10.1023/A:1019863314646
Cross-linked enzyme aggregates with enhanced activity: application to lipases
P. López-Serrano (2004)
10.1016/S0032-9592(01)00237-0
Purification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6
Jong-pil Park (2001)
Immobilized-enzyme activity under isothermal and non-isothermal conditions : studies on β-galactosidase as a model enzyme
D. G. Mita (1995)
10.1021/BM060408+
Glutaraldehyde cross-linking of lipases adsorbed on aminated supports in the presence of detergents leads to improved performance.
G. Fernandez-Lorente (2006)
10.1016/j.enzmictec.2007.08.013
Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins.
I. Estruch (2008)
10.1021/jf801928e
Biocatalysis for the production of industrial products and functional foods from rice and other agricultural produce.
C. Akoh (2008)
10.1016/S0141-0229(02)00169-2
Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters
J. Palomo (2002)
10.1016/0167-7799(85)90104-0
Immobilized biocatalysts — From simple to complex systems
W. Hartmeier (1985)
10.1016/0141-0229(82)90050-3
[Inactivation and reactivation of proteins (enzymes)].
V. Mozhaev (1982)
10.1016/S0009-2509(96)00288-6
Directed evolution: Creating biocatalysts for the future
F. Arnold (1996)
10.1016/0167-7799(94)90018-3
Can immobilization be exploited to modify enzyme activity?
D. Clark (1994)
10.1002/ADSC.200505099
One-pot conversion of cephalosporin C to 7-aminocephalosporanic acid in the absence of hydrogen peroxide
F. López-Gallego (2005)
10.1002/ADSC.200700082
Enzyme Immobilization: The Quest for Optimum Performance
R. Sheldon (2007)
10.1016/J.MOLCATB.2010.10.004
A biocatalytic cascade reaction sensitive to the gas-liquid interface: Modeling and upscaling in a dynamic membrane aeration reactor
W. V. Hecke (2011)
10.1016/S0141-0229(03)00120-0
The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition: Complete hydrolysis of lactose in dairy products
B. Pessela (2003)
10.1038/378159A0
Heterogeneous catalysts obtained by grafting metallocene complexes onto mesoporous silica
T. Maschmeyer (1995)
10.1021/AC00094A001
Sol-gel encapsulation methods for biosensors
Bakul C. Dave (1994)
10.1128/AEM.02107-06
Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Immobilization on Ionic Exchangers
T. Montes (2006)
10.1002/BIT.20107
Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: Evaluation of a novel biocatalyst in organic media
Lorena Wilson (2004)
10.1016/J.JBIOTEC.2006.01.028
Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose.
J. M. Bolivar (2006)
10.1016/J.TIBTECH.2006.12.005
Biocatalysis for pharmaceutical intermediates: the future is now.
D. Pollard (2007)
10.1016/j.cis.2008.06.001
Lipases at interfaces: a review.
P. Reis (2009)
10.1016/S0958-1669(03)00096-X
Immobilised enzymes: carrier-bound or carrier-free?
L. Cao (2003)
10.1016/S0141-0229(02)00070-4
Influence of the enzyme derivative preparation and substrate structure on the enantioselectivity of penicillin G acylase
Silvia Rocchietti (2002)
10.1002/bit.260390306
Production of soluble sugar from cellulosic materials by repeated use of a reversibly soluble‐autoprecipitating cellulase
Machiko Taniguchi (1992)
10.1039/b303307d
Enzymatic transformations. Immobilized A. niger epoxide hydrolase as a novel biocatalytic tool for repeated-batch hydrolytic kinetic resolution of epoxides.
C. Mateo (2003)
10.1016/S0032-9592(03)00288-7
Integrated enzyme purification and immobilization processes with immobilized metal affinity adsorbents
Li-Fan Ho (2004)
10.1002/9780470385869.CH18
Biocatalysis: synthesis of chiral intermediates for drugs.
R. Patel (2006)
10.1016/J.COPBIO.2006.10.009
Nanoscale biocatalyst systems.
P. Wang (2006)
10.1016/S1369-703X(02)00063-3
Lysozyme adsorption and purification by expanded bed chromatography with a small-sized dense adsorbent
Xiao‐Dong Tong (2002)
10.1016/J.PROGPOLYMSCI.2007.01.006
Functional copolymers of N-isopropylacrylamide for bioengineering applications
Z. Rzaev (2007)
10.1016/1381-1169(95)00019-4
Selective oxidation: stabilisation by multipoint attachment of ferredoxin NADP+ reductase, an interesting cofactor recycling enzyme
M. Bes (1995)
10.1002/(SICI)1097-0290(20000405)68:1<98::AID-BIT12>3.0.CO;2-T
Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites.
C. Mateo (2000)
10.1002/bit.22264
Simple strategy of reactivation of a partially inactivated penicillin g acylase biocatalyst in organic solvent and its impact on the synthesis of β‐lactam antibiotics
Oscar Romero (2009)
10.1021/bp0499449
Reversible and Strong Immobilization of Proteins by Ionic Exchange on Supports Coated with Sulfate‐Dextran
M. Fuentes (2004)
10.1074/jbc.M808268200
Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements*
C. Carrasco-López (2009)
10.1002/bit.260170314
Protein hydrolysis with immobilized enzymes
C. C. Detar (1975)
10.1002/btpr.334
Endo‐ and exo‐inulinases: Enzyme‐substrate interaction and rational immobilization
A. Basso (2010)
10.1073/pnas.75.8.3640
A rationale for stabilization of oxygen-labile enzymes: application to a clostridial hydrogenase.
A. Klibanov (1978)
10.1016/J.MOLCATB.2009.12.011
Immobilization of Geobacillus pallidus RAPc8 nitrile hydratase (NHase) reduces substrate inhibition and enhances thermostability
Idan Chiyanzu (2010)
10.1007/s00253-010-2662-8
Engineering of a fungal β-galactosidase to remove product inhibition by galactose
Xuejun Hu (2010)
10.1016/S0165-022X(03)00108-8
A new precursor for the immobilization of enzymes inside sol-gel-derived hybrid silica nanocomposites containing polysaccharides.
Y. A. Shchipunov (2004)
10.1016/S0958-1669(02)00360-9
The production of fine chemicals by biotransformations.
A. J. Straathof (2002)
10.1016/J.ENZMICTEC.2008.10.004
The adsorption of multimeric enzymes on very lowly activated supports involves more enzyme subunits: Stabilization of a glutamate dehydrogenase from Thermus thermophilus by immobilization on heterofunctional supports
J. M. Bolivar (2009)
10.1016/S1381-1177(99)00040-5
Stabilization of enzymes (d-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques
R. Fernández-Lafuente (1999)
10.1016/S0165-022X(01)00207-X
Perspectives of immobilized-metal affinity chromatography.
V. Gaberc-Porekar (2001)
10.1016/S0958-1669(02)00327-0
Assessment of intraparticle biocatalytic distributions as a tool in rational formulation.
J. V. van Roon (2002)
10.1016/J.MOLCATB.2010.09.010
Preparation and characterization of cellulase-bound magnetite nanoparticles
Jason Jordan (2011)
10.1016/S0168-1656(98)00146-1
Preparation, characterization and application of Aspergillus sp. xylanase immobilized on Eudragit S-100.
P. V. Gawande (1998)
10.1016/S0141-0229(01)00308-8
Hydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supports.
E. Lamas (2001)
Local administration of methotrexate bound to activated carbon particles (MTX-CH) for treating cancers in mice.
T. Ito (2003)
10.1016/j.bmc.2008.11.023
Arthrobacter sp. lipase immobilization for preparation of enantiopure masked beta-amino alcohols.
A. C. Chaubey (2009)
10.1016/J.ENZMICTEC.2005.04.021
Increasing the binding strength of proteins to PEI coated supports by immobilizing at high ionic strength
B. Pessela (2005)
10.1093/jb/mvp162
Site-directed mutation at residues near the catalytic site of histamine dehydrogenase from Nocardioides simplex and its effects on substrate inhibition.
Maiko Tsutsumi (2010)
10.1021/BM0493077
Advantages of the pre-immobilization of enzymes on porous supports for their entrapment in sol-gels.
L. Betancor (2005)
10.1002/BIT.260420403
Generation of a pH gradient in an immobilized enzyme system
J. Byers (1993)
10.1016/J.MOLCATB.2005.12.007
Stabilisation of oxygen-labile nitrilases via co-aggregation with poly(ethyleneimine)
C. Mateo (2006)
10.1021/bm100259a
Characterization and study of the orientation of immobilized enzymes by tryptic digestion and HPLC-MS: design of an efficient catalyst for the synthesis of cephalosporins.
C. Temporini (2010)
10.1021/bp049785t
A Simple Strategy for the Purification of Large Thermophilic Proteins Overexpressed in Mesophilic Microorganisms: Application to Multimeric Enzymes from Thermus sp. Strain T2 Expressed in Escherichia coli
B. Pessela (2004)
10.1016/J.MOLCATB.2011.03.008
Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction
Vojtěch Vejvoda (2011)
10.1002/ADSC.201100163
Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes
Rafael C. Rodrigues (2011)
10.1007/s00253-005-0247-8
A multicomponent reaction–diffusion model of a heterogeneously distributed immobilized enzyme
J. V. Roon (2006)
10.1016/j.enzmictec.2010.12.010
High-yield continuous production of nicotinic acid via nitrile hydratase-amidase cascade reactions using cascade CSMRs.
Laura Cantarella (2011)
10.1021/BM025729+
General trend of lipase to self-assemble giving bimolecular aggregates greatly modifies the enzyme functionality.
J. Palomo (2003)
10.1016/S0076-6879(09)63024-X
Protein chromatography on hydroxyapatite columns.
L. J. Cummings (2009)
10.1016/0167-7799(94)90046-9
Purification of proteins by adsorption chromatography in expanded beds.
H. Chase (1994)
10.1016/J.CARBPOL.2010.05.021
Immobilized cellulase by polyvinyl alcohol/Fe2O3 magnetic nanoparticle to degrade microcrystalline cellulose
Hongdong Liao (2010)
10.1111/j.1742-4658.2009.07015.x
Site‐directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase from Bacillus sp. YM55‐1
Vinod Puthan Veetil (2009)
10.1016/j.colsurfb.2009.08.019
Adsorption of cellulase Aspergillus niger on a commercial activated carbon: kinetics and equilibrium studies.
F. Daoud (2010)
10.1016/0022-0728(93)80001-X
A review of the immobilization of enzymes in electropolymerized films
P. N. Barlett (1993)
10.1007/BF02541845
Immobilized lipase reactors for modification of fats and oils—A review
F. Xavier Malcata (1990)
10.1002/(SICI)1097-0290(19990205)62:3<267::AID-BIT3>3.0.CO;2-K
pH gradients in immobilized amidases and their influence on rates and yields of beta-lactam hydrolysis.
A. Spiess (1999)
10.1002/ADSC.200600313
Improved stabilization of genetically modified penicillin G acylase in the presence of organic cosolvents by co-immobilization of the enzyme with polyethyleneimine
T. Montes (2007)
10.1080/10242420500183378
Cross-linked enzyme aggregates (CLEAs): A novel and versatile method for enzyme immobilization (a review)
R. Sheldon (2005)
10.1016/S0141-0229(98)00151-3
Lysozyme inactivation by inert gas bubbling: kinetics in a bubble column reactor
M. Caussette (1999)
10.1002/(SICI)1521-3773(20000117)39:2<380::AID-ANIE380>3.0.CO;2-1
Cofactor-Bound Cross-Linked Enzyme Crystals (CLEC) of Alcohol Dehydrogenase.
St Clair N (2000)
10.1016/j.biortech.2009.11.046
An overview of second generation biofuel technologies.
R. H. Sims (2010)
10.1016/S0167-4838(02)00268-6
Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide.
K. C. Gulla (2002)
10.1038/nature00785
Ordered porous materials for emerging applications
M. Davis (2002)
10.1016/J.AB.2006.01.028
Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder.
S. Shah (2006)
10.1016/j.jbiotec.2009.04.009
Ligands for mixed-mode protein chromatography: Principles, characteristics and design.
Guofeng Zhao (2009)
10.1007/BF02729067
Attempting to remove the substrate inhibition ofL-lactate dehydrogenase fromBacillus stearothermophilus by site-directed mutagenesis
Bariş Binay (2007)
10.1021/BM000072I
Biotransformations catalyzed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions.
R. Fernández-Lafuente (2001)
10.1016/J.ENZMICTEC.2005.10.014
Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins
C. Mateo (2006)
10.1016/J.MOLCATB.2009.04.007
Modulation of a lipase from Staphylococcus warneri EX17 using immobilization techniques
G. Volpato (2009)
10.1021/BM020086J
One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads.
B. Pessela (2003)
10.1002/bit.22042
Bubble‐free oxygenation of a bi‐enzymatic system: effect on biocatalyst stability
W. Van Hecke (2009)
10.2174/092986710793205435
Use of enzymes in the production of semi-synthetic penicillins and cephalosporins: drawbacks and perspectives.
G. Volpato (2010)
10.1016/J.PROCBIO.2008.06.004
Enzyme stability and stabilization—Aqueous and non-aqueous environment
Padma Iyer (2008)
10.1016/S0378-4347(98)00434-4
Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function.
J. Turková (1999)
10.1016/S1631-0748(03)00006-7
Mesoporous alginate/silica biocomposites for enzyme immobilisation
T. Coradin (2003)
10.1039/b711564b
Understanding enzyme immobilisation.
U. Hanefeld (2009)
10.1021/AR990017Q
High-performance nanocatalysts for single-step hydrogenations.
J. Thomas (2003)
10.1016/J.MOLCATB.2008.12.010
Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization
J. M. Bolivar (2009)
10.1021/LA0115177
Enzymatic Formation of pH Gradients within Polyelectrolyte Gels with Immobilized Urease
Kazuyoshi Ogawa and (2002)
10.1016/J.PROCBIO.2009.03.012
The co-operative effect of physical and covalent protein adsorption on heterofunctional supports
J. M. Bolivar (2009)
10.1016/S1381-1177(02)00180-7
Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding enzyme molecules: New enzyme derivatives to be used in any reaction medium
O. Abián (2002)
10.1021/BM0343895
Co-aggregation of penicillin g acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media.
Lorena Wilson (2004)
10.1021/BM060527P
Chemical modification of protein surfaces to improve their reversible enzyme immobilization on ionic exchangers.
T. Montes (2006)
10.1016/S0308-8146(00)00216-8
Partial purification and characterisation of a xylanase enzyme produced by a micro-organism isolated from selected indigenous fruits of Zimbabwe
Ernest T. Chivero (2001)
10.1007/S11746-998-0096-6
Production of specific-structured lipids by enzymatic interesterification in a pilot continuous enzyme bed reactor
X. Xu (1998)
10.1271/bbb1961.53.2909
Co-immobilization of Alcohol Dehydrogenase, Diaphorase and NAD and Its Application to Flow Injection Analytical System for Ethanol
H. Ukeda (1989)
10.1016/J.JFOODENG.2004.09.005
Performance of immobilized pectinex ultra SP-L on magnetic duolite-polystyrene composite particles. Part II: A magnetic fluidized bed reactor study
Devlet Demirel (2005)
10.1016/J.ENZMICTEC.2005.10.015
Effect of lipase–lipase interactions in the activity, stability and specificity of a lipase from Alcaligenes sp.
L. Wilson (2006)
10.1021/CR0204707
Strategies in making cross-linked enzyme crystals
Joseph J. Roy (2004)
10.1016/S0376-7388(00)00342-2
Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review
D. Butterfield (2001)
10.1016/J.JFOODENG.2003.09.018
Performance of immobilized Pectinex Ultra SP-L on magnetic duolite-polystyrene composite particles Part I: a batch reactor study
Devlet Demirel (2004)
10.1016/J.ENZMICTEC.2006.04.019
Selective adsorption of large proteins on highly activated IMAC supports in the presence of high imidazole concentrations: Purification, reversible immobilization and stabilization of thermophilic α- and β-galactosidases
B. Pessela (2007)
10.1007/978-1-59745-053-9
Immobilization of enzymes and cells
J. M. Guisan (2006)
10.1016/j.tibtech.2008.03.004
New opportunities for biocatalysis: making pharmaceutical processes greener.
J. Woodley (2008)
10.1016/S0021-9673(99)01008-0
Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose.
G. Chaga (1999)
10.1016/S0167-7799(00)01493-1
Bioencapsulation within synthetic polymers (Part 2): non-sol-gel protein-polymer biocomposites.
I. Gill (2000)
10.1016/S0009-3084(98)00042-5
Immobilization of lipases by selective adsorption on hydrophobic supports.
R. Fernández-Lafuente (1998)
10.1002/BIT.10560
Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties.
G. Fernandez-Lorente (2003)
10.1002/BIT.260480505
Review: Multipoint binding and heterogeneity in immobilized metal affinity chromatography.
R. D. Johnson (1995)



This paper is referenced by
10.1080/02603594.2015.1059827
Immobilization of Protein on Nanoporous Metal-Organic Framework Materials
D. Raja (2015)
10.1002/tcr.201500008
Recent Advances in Biocatalytic Promiscuity: Hydrolase-Catalyzed Reactions for Nonconventional Transformations.
M. López-Iglesias (2015)
10.1016/j.colsurfb.2012.07.003
A novel approach for efficient immobilization and stabilization of papain on magnetic gold nanocomposites.
Banalata Sahoo (2013)
10.1002/JCTB.3919
Resolution of N‐hydroxymethyl vince lactam catalyzed by lipase in organic solvent
Erna Xun (2013)
10.1016/j.talanta.2014.04.050
Quantum dots and p-phenylenediamine based method for the sensitive determination of glucose.
Xiaoxiao Lv (2014)
10.1007/978-3-319-13521-2_23
Solid-Binding Peptides: Immobilisation Strategies for Extremophile Biocatalysis in Biotechnology
Andrew Care (2016)
10.1016/j.ijbiomac.2015.12.018
Preparation of lactoperoxidase incorporated hybrid nanoflower and its excellent activity and stability.
Cevahir Altinkaynak (2016)
10.3390/molecules21060767
Tailoring the Spacer Arm for Covalent Immobilization of Candida antarctica Lipase B—Thermal Stabilization by Bisepoxide-Activated Aminoalkyl Resins in Continuous-Flow Reactors
Emese Abaházi (2016)
10.3390/molecules22010091
Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites
Jose J Virgen-Ortiz (2017)
10.1021/acsami.7b02803
Immobilization on Metal-Organic Framework Engenders High Sensitivity for Enzymatic Electrochemical Detection.
C. Zhang (2017)
10.1021/acsami.6b13632
Immobilization of 2-Deoxy-d-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique.
Stefan Reinicke (2017)
10.1016/J.IBIOD.2017.07.012
Chitosan based metal-chelated copolymer nanoparticles: Laccase immobilization and phenol degradation studies
Erol Alver (2017)
10.1007/978-981-13-2257-0_9
Carrier-free enzyme immobilization by cross-linked enzyme aggregates (CLEA) technology
Faridah Yusof (2018)
10.3390/CATAL8080299
Physiochemical Characterization of α-Amylase as Crosslinked Enzyme Aggregates
Xiaodong Li (2018)
10.3390/CATAL8110496
Preparation of Magnetic Cross-Linked Amyloglucosidase Aggregates: Solving Some Activity Problems
Murilo Amaral-Fonseca (2018)
10.1039/C8RA07559J
Characterization of immobilized tyrosinase – an enzyme that is stable in organic solvent at 100 °C
Lidong Wu (2018)
10.1007/s13205-019-2028-6
Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate
J. Zheng (2020)
10.1016/j.enzmictec.2020.109535
Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
Sara Arana-Peña (2020)
10.3390/catal10040410
Design and Construction of an Effective Expression System with Aldehyde Tag for Site-Specific Enzyme Immobilization
Fang Wang (2020)
10.1007/s13738-019-01773-5
An enzymatic performance for a new swift magnetically detachable bio-conjugate of Candida rugosa lipase with modified Fe3O4–graphene oxide nanocomposite
Faranak Jafarian (2019)
10.1016/j.biortech.2019.121548
Improving biocatalysis of cefaclor with penicillin acylase immobilized on magnetic nanocrystalline cellulose in deep eutectic solvent based co-solvent.
Xiao-ling Wu (2019)
10.1007/s11164-019-03937-7
Immobilization of horseradish peroxidase on polyglycerol-functionalized magnetic Fe3O4/nanodiamond nanocomposites and its application in phenol biodegradation
Anxia Li (2019)
10.1080/10826068.2019.1587625
Improvement of lipase activity by synergistic immobilization on polyurethane and its application for large-scale synthesizing vitamin A palmitate
Caixia Cui (2019)
10.1016/j.aca.2017.02.009
Papain-functionalized gold nanoparticles as heterogeneous biocatalyst for bioanalysis and biopharmaceuticals analysis.
Siyao Liu (2017)
10.1002/btpr.1571
Crosslinked aggregates of Rhizopus oryzae lipase as industrial biocatalysts: Preparation, optimization, characterization, and application for enantioselective resolution reactions
F. Kartal (2012)
10.1016/J.MOLCATB.2013.05.018
The study on effective immobilization of lipase on functionalized bentonites and their properties
Huaping Dong (2013)
10.1016/j.enzmictec.2015.12.003
Kinetic modeling and docking study of immobilized lipase catalyzed synthesis of furfuryl acetate.
A. C. Mathpati (2016)
10.3390/membranes2020198
Immobilization of Mucor miehei Lipase onto Macroporous Aminated Polyethersulfone Membrane for Enzymatic Reactions
Nurrahmi Handayani (2012)
10.1016/J.PROCBIO.2012.04.019
Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica
Oveimar Barbosa (2012)
10.1016/J.PROCBIO.2017.06.014
Coimmobilization of enzymes in bilayers using pei as a glue to reuse the most stable enzyme: Preventing pei release during inactivated enzyme desorption
Hadjer Zaak (2017)
10.1016/J.BEJ.2012.04.009
Reversible immobilization of glucoamylase onto metal–ligand functionalized magnetic FeSBA-15
G. Zhao (2012)
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