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Medium Development For Enhanced Production Of Alkaline Protease From A Newly Isolated Bacillus Pseudofirmus SVB1

S. Sen, V. V. Dasu, B. Mandal
Published 2010 · Chemistry

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The most significantly influencing medium components viz., casein, MgSO4, CaCl2, and NH4NO3 were selected to maximize the production of alkaline protease (specific activity) from a newly isolated Bacillus pseudofirmus SVB1 using Plackett–Burman experimental design. Central composite design was used to determine the optimal levels of these screened medium components. The optimal levels of casein, MgSO4, CaCl2, and NH4NO3 were found to be 7.636, 0.811, 0.086, and 0.576 g/l, respectively. An overall 4.89-fold increase in alkaline protease production (specific activity) was achieved in the optimized medium. Copyright © 2009 Curtin University of Technology and John Wiley & Sons, Ltd.
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