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(-)-Epigallocatechin-3-gallate Suppresses Osteoclast Differentiation And Ameliorates Experimental Arthritis In Mice.

A. Morinobu, Wang Biao, S. Tanaka, Marika Horiuchi, L. Jun, Goh Tsuji, Y. Sakai, M. Kurosaka, S. Kumagai
Published 2008 · Medicine

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OBJECTIVE To verify the effects of (-)-epigallocatechin-3-gallate (EGCG) on osteoclast differentiation and on experimental arthritis in mice. METHODS Human osteoclasts were differentiated from peripheral blood monocytes. The effects of EGCG were examined by tartrate-resistant acid phosphatase (TRAP) staining, bone resorption assay, Western blotting, and quantitative real-time polymerase chain reaction. Arthritis was induced in mice by injecting a cocktail of monoclonal antibodies against collagen. EGCG (20 microg/gm body weight) was administered intraperitoneally every day from day 0 through the end of the experiments (day 15). The effects of EGCG were determined by assessments of joint swelling, histologic changes, and TRAP staining on day 15. RESULTS EGCG reduced the generation of TRAP-positive multinucleated cells, bone resorption activity, and osteoclast-specific gene expression without affecting cell viability. EGCG down-regulated expression of nuclear factor of activated T cells c1 (NF-ATc1), but not of NF-kappaB, c-Fos, and c-Jun, suggesting that down-regulation of NF-ATc1 is one of the molecular bases of EGCG action. Additionally, EGCG treatment ameliorated clinical symptoms and reduced histologic scores in arthritic mice (P < 0.05). The in vivo effect of EGCG on osteoclast differentiation was not clear in this model, probably because EGCG suppressed the inflammation itself. CONCLUSION EGCG suppressed osteoclast differentiation and ameliorated experimental arthritis in mice over the short term. It remains to be established whether EGCG is useful for the prevention and treatment of osteoporosis and rheumatoid arthritis.
This paper references
10.1016/J.GENE.2005.01.014
Regulatory roles and molecular signaling of TNF family members in osteoclasts.
X. Feng (2005)
10.1016/J.MRFMMM.2005.04.010
Modulation of signal transduction by tea catechins and related phytochemicals.
Masahito Shimizu (2005)
10.4049/jimmunol.170.8.4335
Neutrophil Restraint by Green Tea: Inhibition of Inflammation, Associated Angiogenesis, and Pulmonary Fibrosis1
M. Donà (2003)
10.1016/S1534-5807(02)00369-6
Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts.
H. Takayanagi (2002)
10.1002/ART.10443
Epigallocatechin‐3‐gallate inhibits interleukin‐1β–induced expression of nitric oxide synthase and production of nitric oxide in human chondrocytes: Suppression of nuclear factor κB activation by degradation of the inhibitor of nuclear factor κB
R. Singh (2002)
10.1080/08916930601167343
Green tea polyphenols reduce autoimmune symptoms in a murine model for human Sjogren's syndrome and protect human salivary acinar cells from TNF-α-induced cytotoxicity
Stephen Hsu (2007)
10.1016/J.FREERADBIOMED.2006.01.006
Thioredoxin protects against joint destruction in a murine arthritis model.
Goh Tsuji (2006)
10.1002/j.1460-2075.1987.tb04762.x
Expression and rescuing of a cloned human tumour necrosis factor gene using an EBV‐based shuttle cosmid vector.
D. Kioussis (1987)
10.1097/01.bor.0000231912.24740.a5
Osteoclasts, rheumatoid arthritis, and osteoimmunology
K. Sato (2006)
10.4049/jimmunol.173.9.5794
Green Tea Epigallocatechin-3-Gallate Mediates T Cellular NF-κB Inhibition and Exerts Neuroprotection in Autoimmune Encephalomyelitis1
O. Aktas (2004)
10.1016/J.LFS.2005.12.006
Green tea and its polyphenolic catechins: medicinal uses in cancer and noncancer applications.
N. Zaveri (2006)
10.1038/nrg1122
Genetic regulation of osteoclast development and function
S. Teitelbaum (2003)
10.1016/S1525-0016(03)00235-1
A therapeutic strategy uses histone deacetylase inhibitors to modulate the expression of genes involved in the pathogenesis of rheumatoid arthritis.
Y. Chung (2003)
10.1038/nature01661
Evolving concepts of rheumatoid arthritis
G. Firestein (2003)
10.1172/JCI15582
Osteoclasts are essential for TNF-α–mediated joint destruction
K. Redlich (2002)
10.1016/S0002-9440(10)63016-7
TRANCE/RANKL knockout mice are protected from bone erosion in a serum transfer model of arthritis.
A. Pettit (2001)
10.1016/0006-2952(86)90391-6
Inhibition of bone resorption in culture by (+)-catechin.
J. M. Delaissé (1986)
Induction of arthritis with monoclonal antibodies to collagen.
K. Terato (1992)
10.1210/EN.2005-0451
Prostaglandin E2 strongly inhibits human osteoclast formation.
I. Take (2005)
Role of gamma delta T cells in murine collagen-induced arthritis.
G. Peterman (1993)
10.1172/JCI19112
CD69 downregulates autoimmune reactivity through active transforming growth factor-beta production in collagen-induced arthritis.
D. Sancho (2003)
10.1016/J.MOLMED.2005.11.007
RANKL-RANK signaling in osteoclastogenesis and bone disease.
T. Wada (2006)
10.1186/ar2046
Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts
Y. Komano (2006)
10.1182/BLOOD-2002-08-2622
Two histone deacetylase inhibitors, trichostatin A and sodium butyrate, suppress differentiation into osteoclasts but not into macrophages.
M. Rahman (2003)
10.1002/ART.22023
Regulation of interleukin-1β–induced chemokine production and matrix metalloproteinase 2 activation by epigallocatechin-3-gallate in rheumatoid arthritis synovial fibroblasts
S. Ahmed (2006)
10.1002/ART.21211
RANK and RANKL expression as markers of dendritic cell-T cell interactions in paired samples of rheumatoid synovium and lymph nodes.
G. Page (2005)
10.1002/jcb.21104
(−)‐epigallocatechin gallate enhances prostaglandin F2α‐induced VEGF synthesis via upregulating SAPK/JNK activation in osteoblasts
H. Tokuda (2007)
10.1002/1529-0131(200105)44:5<1003::AID-ANR179>3.0.CO;2-#
Activated human T cells directly induce osteoclastogenesis from human monocytes: possible role of T cells in bone destruction in rheumatoid arthritis patients.
S. Kotake (2001)
10.1038/ncprheum0036
Mechanisms of Disease: the link between RANKL and arthritic bone disease
G. Schett (2005)
10.1038/nrc1189
Cancer chemoprevention with dietary phytochemicals
Young-Joon Surh (2003)
10.1007/s00198-005-1995-0
Green tea catechin enhances osteogenesis in a bone marrow mesenchymal stem cell line
C. Chen (2005)
Trichostatin A cooperates with Fas-mediated signal to induce apoptosis in rheumatoid arthritis synovial fibroblasts.
A. Morinobu (2006)
10.1158/0008-5472.CAN-05-3636
Targeting multiple signaling pathways by green tea polyphenol (-)-epigallocatechin-3-gallate.
N. Khan (2006)



This paper is referenced by
10.1016/j.ajpath.2011.09.007
Epigallocatechin-3-gallate ameliorates experimental autoimmune encephalomyelitis by altering balance among CD4+ T-cell subsets.
J. Wang (2012)
10.3945/ajcn.113.058255
Tea and bone health: steps forward in translational nutrition.
Chwan-Li Shen (2013)
n vivo anti-arthritic and antioxidant effects from the standardized thanolic extract of Moussonia deppeana abriel
A. Gutiérrez-Rebolledoa (2018)
10.21776/ub.eprodenta.2018.002.01.5
EFEK EKSTRAK DAUN TEH HIJAU (Camellia sinensis) TERHADAP SEL OSTEOKLAS TULANG ALVEOLAR TIKUS PUTIH (Rattus norvegicus)
N. Prasetyaningrum (2018)
10.1016/j.exger.2014.05.008
EGCG mediated downregulation of NF-AT and macrophage infiltration in experimental hepatic steatosis
Thulasi Raman Krishnan (2014)
10.1016/j.lfs.2010.04.013
Green tea polyphenol epigallocatechin-3-gallate: inflammation and arthritis. [corrected].
R. Singh (2010)
Green tea polyphenol epigallocatechi 3-gallate : In fl ammation and arthritis
R. Singh (2010)
10.3390/molecules24081589
Could Polyphenols Help in the Control of Rheumatoid Arthritis?
S. Sung (2019)
10.1371/journal.pone.0086062
EGCG Attenuates Autoimmune Arthritis by Inhibition of STAT3 and HIF-1α with Th17/Treg Control
Eun-ji Yang (2014)
10.1007/978-3-030-37297-2_13
Antioxidants, Therapeutic Options, and Regulation of the Immune Responses
Loutfy H. Madkour (2020)
Cymbopogon citratus and its polyphenols as potential phytotherapeutic products: an in vivo approach
G. F. Costa (2016)
10.1007/s40496-020-00261-7
Nutritional Supplements and Periodontal Disease Prevention—Current Understanding
Y. Aoki-Nonaka (2020)
10.1155/2008/291808
(-)-Epigallocatechin Gallate Reduces Platelet-Derived Growth Factor-BB-Stimulated Interleukin-6 Synthesis in Osteoblasts: Suppression of SAPK/JNK
S. Takai (2008)
10.2174/092986712800672049
Intracellular signaling pathways modulated by phenolic compounds: application for new anti-inflammatory drugs discovery.
G. Costa (2012)
10.1016/j.pharmthera.2018.09.009
The role of flavonoids in autoimmune diseases: Therapeutic updates
Kannan R R Rengasamy (2019)
10.1002/jsfa.8664
Anti-inflammatory effects of polyphenols in arthritis.
F. Oliviero (2018)
10.1016/j.jnutbio.2011.02.006
Dietary supplementation with high dose of epigallocatechin-3-gallate promotes inflammatory response in mice.
M. Pae (2012)
10.1111/j.1600-0765.2008.01198.x
Green tea catechin inhibits lipopolysaccharide-induced bone resorption in vivo.
H. Nakamura (2010)
Epigallocatechin-3-Gallate Directly Suppresses T Cell Proliferation through Impaired IL-2 Utilization and Cell Cycle Progression 1 – 3
M. Pae (2010)
Cationic Lipid Nanomedicines for the treatment of Diabetic retinopathy
J. Fangueiro (2015)
10.1016/J.BJP.2018.02.004
In vivo anti-arthritic and antioxidant effects from the standardized ethanolic extract of Moussonia deppeana
G. A. Gutiérrez-Rebolledo (2018)
10.1186/ar2982
Green tea polyphenol epigallocatechin 3-gallate in arthritis: progress and promise
S. Ahmed (2010)
10.1016/j.imlet.2013.11.006
Epigallocatechin-3-gallate ameliorates both obesity and autoinflammatory arthritis aggravated by obesity by altering the balance among CD4+ T-cell subsets.
Jae-Kyeong Byun (2014)
10.1007/s00109-012-0964-2
Green tea epigallocatechin-3-gallate modulates differentiation of naïve CD4+ T cells into specific lineage effector cells
J. Wang (2012)
10.1016/j.joca.2009.08.015
Inhibition of cyclooxygenase-2 expression and prostaglandin E2 production in chondrocytes by avocado soybean unsaponifiables and epigallocatechin gallate.
L. F. Heinecke (2010)
10.1124/jpet.116.238444
Allylpyrocatechol Attenuates Collagen-Induced Arthritis via Attenuation of Oxidative Stress Secondary to Modulation of the MAPK, JAK/STAT, and Nrf2/HO-1 Pathways
S. De (2017)
10.1002/jbm.b.31848
Implantation of green tea catechin α-tricalcium phosphate combination enhances bone repair in rat skull defects.
R. Rodriguez (2011)
10.1016/j.foodchem.2012.03.002
Evaluation of the anti-inflammatory effects of phloretin and phlorizin in lipopolysaccharide-stimulated mouse macrophages.
Wei-tien Chang (2012)
10.1155/2017/5813793
Therapeutic Potential of Epigallocatechin Gallate Nanodelivery Systems
Andreia Granja (2017)
10.1254/JPHS.11082FP
Tea polyphenols inhibit rat osteoclast formation and differentiation.
Y. Oka (2012)
Anti-aging and anti-osteoporosis effects of green teen polyphenol in a premature aging model of Bmi-1 knockout mice
Yuanqing Huang (2017)
Potential bene fi ts of green tea polyphenol EGCG in the prevention and treatment of vascular in fl ammation in rheumatoid arthritis
Sharayah Riegsecker (2013)
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