← Back to Search
Micronization Of Lysozyme By Supercritical Assisted Atomization
R. Adami, L. S. Osséo, E. Reverchon
Published 2009 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
Supercritical Assisted Atomization (SAA) has been used to produce lysozyme microparticles. Lysozyme has been micronized using water, buffered water at pH 6.2 and water–ethanol mixtures at different volume percentages. Precipitated lysozyme particles were spherical, with a narrow particle size distribution (PSD) ranging from 0.1 to 4 µm. The concentration of lysozyme in the liquid solvent mixture had a nonlinear effect on the particle distribution, with an increase of the X0.9 from about 1 to 3 µm varying the enzyme concentration from 5 to 20 mg/mL. Precipitation temperature was set as low as possible to avoid enzyme degradation. High‐performance liquid chromatography analysis showed no degradation of lysozyme and the enzyme activity, measured by turbidimetric enzymatic assay, only slightly decreased after SAA processing. Depending on the process conditions lysozyme retained from 95% to 100% of the biological activity compared to the untreated enzyme. Biotechnol. Bioeng. 2009; 104: 1162–1170. © 2009 Wiley Periodicals, Inc.
This paper references
Effect of process conditions on recovery of protein activity after freezing and freeze-drying.
S. Jiang (1998)
Cyclodextrins micrometric powders obtained by supercritical fluid processing
E. Reverchon (2006)
Densities and P-x-y diagrams for carbon dioxide dissolution in methanol, ethanol, and acetone mixtures
C. Chang (1997)
The effect of a water/organic solvent interface on the structural stability of lysozyme.
M. van de Weert (2000)
Science and Engineering of Droplets:: Fundamentals and Applications
Huimin Liu (1999)
Gas-liquid equilibrium for ethanol-water-carbon dioxide mixtures at elevated pressures
M. L. Gilbert (1986)
Precipitation of Lysozyme Nanoparticles from Dimethyl Sulfoxide Using Carbon Dioxide as Antisolvent
G. Muhrer (2003)
Resonance scattering spectra of micrococcus lysodeikticus and its application to assay of lysozyme activity.
Z. Jiang (2007)
Lyophilization and development of solid protein pharmaceuticals.
W. Wang (2000)
Effect of preparation temperature on the characteristics and release profiles of PLGA microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method.
Y. Yang (2000)
Extraction of enzymes by reverse micelles
G. A. Krei (1992)
The mutual solubilities of water with supercritical and liquid carbon dioxides
M. B. King (1992)
The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme.
D. Shugar (1952)
Protein structure by Fourier transform infrared spectroscopy: second derivative spectra.
H. Susi (1983)
Supercritical-assisted atomization to produce micro- and/or nanoparticles of controlled size and distribution
E. Reverchon (2002)
Protein nanoparticles formation by supercritical antisolvent with enhanced mass transfer
P. Chattopadhyay (2002)
Dense gas anti-solvent processes for pharmaceutical formulation
F. Dehghani (2003)
Supercritical fluid processing of proteins. I: lysozyme precipitation from organic solution.
S. Moshashaée (2000)
Bioavailability of pulmonary delivered peptides and proteins: α-interferon, calcitonins and parathyroid hormones
J. Patton (1994)
Precipitation of proteins in supercritical carbon dioxide.
M. Winters (1996)
Supercritical fluid processing of proteins: lysozyme precipitation from aqueous solution
S. Moshashaée (2003)
Application of supercritical fluid to preparation of powders of high-molecular weight drugs for inhalation.
H. Okamoto (2008)
Role of phase behavior in micronization of lysozyme via a supercritical anti-solvent process
Shan-Chun Chang (2008)
Liquid–liquid extraction of biomolecules: an overview and update of the main techniques
P. Mazzola (2008)
Protein extraction using reversed micelles
R. Hilhorst (1992)
Spherical microparticles production by supercritical antisolvent precipitation: Interpretation of results
E. Reverchon (2008)
Effects of types of sugar on the stabilization of protein in the dried state.
K. Imamura (2003)
Protein Inhalation Powders: Spray Drying vs Spray Freeze Drying
Y. Maa (2004)
Protein formulation and lyophilization cycle design: prevention of damage due to freeze-concentration induced phase separation.
M. Heller (1999)
Production of cromolyn sodium microparticles for aerosol delivery by supercritical assisted atomization
E. Reverchon (2008)
Fourier transform infrared spectroscopic analysis of protein secondary structures.
J. Kong (2007)
Supercritical assisted atomization: Performance comparison between laboratory and pilot scale
E. Reverchon (2006)
Lysozyme particle formation during supercritical fluid drying: Particle morphology and molecular integrity
A. Bouchard (2007)
Polymer microparticles production by supercritical assisted atomization
E. Reverchon (2007)
Protein Spray-Freeze Drying. Effect of Atomization Conditions on Particle Size and Stability
H. Costantino (2004)
Griseofulvin micronization and dissolution rate improvement by supercritical assisted atomization
E. Reverchon (2004)
Dry powders of stable protein formulations from aqueous solutions prepared using supercritical CO(2)-assisted aerosolization.
S. Sellers (2001)
Spray freezing into liquid versus spray-freeze drying: influence of atomization on protein aggregation and biological activity.
Zhongshui Yu (2006)
Supercritical assisted atomization: A novel technology for microparticles preparation of an asthma-controlling drug
G. D. Porta (2008)
Effects of drying methods and additives on the structure, function, and storage stability of subtilisin: role of protein conformation and molecular mobility
R. A. DePaz (2002)
Protein secondary structures in water from second-derivative amide I infrared spectra.
A. Dong (1990)
The Binary System Carbon Dioxide-Water under Pressure.
R. Wiebe (1941)
Effect of different modes of inhalation on drug delivery from a dry powder inhaler
S. Newman (1994)
Stabilization of Proteins in Dry Powder Formulations Using Supercritical Fluid Technology
N. Jovanović (2004)
Terbutaline microparticles suitable for aerosol delivery produced by supercritical assisted atomization.
E. Reverchon (2003)
Solvent effects on the controlled dense gas precipitation of model proteins
R. Thiering (2000)
Precipitation of lysozyme solubilized in reverse micelles by dissolved CO2
H. Zhang (2001)
Particle Design Using Supercritical Fluids
E. Reverchon (2003)
(D) Routes of delivery: Case studies
J. Patton (1992)
Solubility of carbon dioxide in binary and ternary mixtures with ethanol and water
I. Dalmolin (2006)
This paper is referenced by
iii Development of chitosan-based microparticles for pulmonary drug delivery
R. P. Cabral (2013)
Comparison of Flavonoids from Spina Gleditsiae by Response Surface Method and Orthogonal Design
Xiaojuan Liu (2019)
Supercritical Assisted Atomization: effect of operative conditions on PVP microparticle size and morphology
Sara Liparoti (2015)
Aplicación de procesos de separación con co2 supercrítico a la producción y optimización de bioplaguicidas
J. L. Navarro (2011)
Supercritical fluid assisted production of chitosan oligomers micrometric powders.
Z. Du (2014)
Supercritical Assisted Atomization of emulsions for encapsulation of 1-monoacylglycerols in an hydrophilic carrier
Ševčíková Petra (2015)
Supercritical fluid assisted atomization introduced by an enhanced mixer for micronization of lysozyme: Particle morphology, size and protein stability.
Z. Du (2011)
Supercritical Assisted Atomization: Polyvinylpyrrolidone as Carrier for Drugs with Poor Solubility in Water
Sara Liparoti (2013)
Nanoparticle precipitation by Supercritical Assisted Injection in a Liquid Antisolvent
R. Campardelli (2012)
Synthesis of nanostructured materials using supercritical CO2: Part I. Physical transformations
D. Sanli (2011)
Development of chitosan-based microparticles for pulmonary drug delivery
Grau de Mestre (2013)
Use of Supercritical Assisted Atomization to produce nanoparticles from olive pomace extract
B. Aliakbarian (2017)
Production of lysozyme microparticles to be used in functional foods, using an expanded liquid antisolvent process
V. Prosapio (2016)
Lincomycin hydrochloride loaded albumin microspheres for controlled drug release, produced by Supercritical Assisted Atomization
R. Adami (2017)
Recent Progress of Supercritical Carbon Dioxide in Producing Natural Nanomaterials.
Maobin Xie (2019)
PLA–PEG copolymers micronization by supercritical assisted atomization
R. Adami (2012)
Supercritical fluid assisted production of micrometric powders of the labile trypsin and chitosan/trypsin composite microparticles.
Yu-Bin Shen (2015)
Supercritical Assisted Atomization for the production of curcumin-biopolymer microspheres
R. Adami (2017)
Preparation of chitosan microparticles with diverse molecular weights using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer
Yu-Bin Shen (2014)
Supercritical methodologies applied to the production of biopesticides: a review
L. Martín (2012)
Bioactive insulin microparticles produced by supercritical fluid assisted atomization with an enhanced mixer.
Zhe Du (2013)
A new supercritical assisted atomization configuration, for the micronization of thermolabile compounds
R. Adami (2011)
Design and production of gentamicin/dextrans microparticles by supercritical assisted atomisation for the treatment of wound bacterial infections.
R. Aquino (2013)
Dense CO2 as a Solute, Co-Solute or Co-Solvent in Particle Formation Processes: A Review
Ana V. M. Nunes (2011)
Composite polymer-Fe3O4 microparticles for biomedical applications, produced by Supercritical Assisted Atomization
R. Adami (2012)
Rotenone coprecipitation with biodegradable polymers by supercritical assisted atomization
L. Martín (2013)
Perspectives on the use of supercritical particle formation technologies for food ingredients
F. Temelli (2017)
Supercritical assisted atomization to produce nanostructured chitosan-hydroxyapatite microparticles for biomedical application
E. Reverchon (2013)
Controllable preparation and formation mechanism of BSA microparticles using supercritical assisted atomization with an enhanced mixer
Q. Wang (2011)
Supercritical fluids applications in nanomedicine
R. Campardelli (2015)
Sustainable strategies for nano-in-micro particle engineering for pulmonary delivery
A. S. Silva (2014)