Online citations, reference lists, and bibliographies.

Dynamic Light-scattering Analysis Of The Electrostatic Interaction Of Hexahistidine-tagged Cytochrome P450 Enzyme With Semiconductor Quantum Dots.

Binil Itty Ipe, Anuj Shukla, Huachang Lu, Bo Zou, Heinz Rehage, Christof M. Niemeyer
Published 2006 · Chemistry, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Currently, there is great interest in the development of methods suitable for determining the stoichiometry of biomolecules attached to nanoparticles. We describe the use of the dynamic light-scattering technique (DLS) to determine the stoichiometry of the protein cytochrome P450(BSbeta) attached to CdS and CdSe quantum dots (QDs). The enzyme-conjugated QDs have different diffusion characteristics compared to the QD and enzyme precursors, expressed in their size, scattering intensity as well as zeta-potential values. The significant enhancement of the scattering intensity of QDs observed upon conjugation with the P450(BSbeta) due to the refractive-index increment and the systematic variation in zeta potential resulting from charge neutralization of the anionic QDs by the cationic histidine-tagged P450(BSbeta) have been used for stoichiometry determination.

This paper is referenced by
Lead (II) ion detection in surface water with pM sensitivity using aza-crown-ether-modified silver nanoparticles via dynamic light scattering.
Liang Zhang (2011)
Bio-functionalised nanoparticles for enzyme sensing
James E. Ghadiali (2010)
Biological characterization of nanomaterials
Anita Jemec (2015)
In Vivo Retargeting of Poly(beta aminoester) (OM-PBAE) Nanoparticles is Influenced by Protein Corona.
Cristina Fornaguera (2019)
Studies on Interaction of CdTe Quantum Dots with Bovine Serum Albumin Using Fluorescence Correlation Spectroscopy
Liwen Shao (2008)
Quantum Dots Bioconjugates
Xiaoze Jiang (2014)
Use of gold nanoparticles in a simple colorimetric and ultrasensitive dynamic light scattering assay: selective detection of arsenic in groundwater.
Jhansi Rani Kalluri (2009)
CdTe quantum dots - preparation, physico-chemical propertiesand application as fluorescent markers
Ivona Svobodová (2009)
Analytical strategies for detecting nanoparticle-protein interactions.
Liwen Li (2010)
Thioglycolic acid-capped ZnSe quantum dots as nanoprobe for cobalt(II) and iron(III) via measurement of grey level, UV-vis spectra and dynamic light scattering
Xinxin Xing (2019)
Control of Surface Ligand Density on PEGylated Gold Nanoparticles for Optimized Cancer Cell Uptake
Hongying Liu (2015)
Interaction of Globular Plasma Proteins with Water-Soluble CdSe Quantum Dots.
Jyotsana Pathak (2015)
Recent progress in the bioconjugation of quantum dots
Juan B. Blanco-Canosa (2014)
Site-specific functionalization of anisotropic nanoparticles: from colloidal atoms to colloidal molecules.
Fan Li (2009)
Rational nanoconjugation improves biocatalytic performance of enzymes: aldol addition catalyzed by immobilized rhamnulose-1-phosphate aldolase.
Inés Ardao (2012)
Photocatalytic activity of colloidal CdS nanoparticles with different capping ligands
Vidyalakshmi Rajendran (2009)
Investigation the interaction between protamine sulfate and CdTe quantum dots with spectroscopic techniques
Fangfang Xue (2016)
Protein-nanoparticle interactions: opportunities and challenges.
Morteza Mahmoudi (2011)
Interaction of Water-Soluble CdTe Quantum Dots with Bovine Serum Albumin
Vilius Poderys (2011)
Interaction of CdTe Nanorods with Bovine Serum Albumin
Swades Ranjan Bera (2016)
Live Cell Labeling of Glial Progenitor Cells Using Targeted Quantum Dots
Nidhi Sadana Sabharwal (2009)
Sensitive immunoassay for porcine pseudorabies antibody based on fluorescence signal amplification induced by cation exchange in CdSe nanocrystals
Xuepu Li (2012)
The role of PEG conformation in mixed layers: from protein corona substrate to steric stabilization avoiding protein adsorption
Joan Comenge (2015)
Sensitive Determination for Papain Conjugated CdSe Quantum Dots by Dynamic Light Scattering Analysis
Arup Ratan Mandal (2015)
Enzyme nanoarchitectonics: organization and device application.
Katsuhiko Ariga (2013)
A multidimensional sensing device for the discrimination of proteins based on manganese-doped ZnS quantum dots.
Peng Wu (2011)
Controlled adsorption of cytochrome c to nanostructured gold surfaces
Inês Gomes (2012)
Recent Progress Toward the Spectroscopic Analysis of Biomacromolecule–Nanoparticle Interactions
Zhili Peng (2017)
12. Biological Characterization of Nanomaterials
Anita Jemec (2016)
The nanoparticle biomolecule corona: lessons learned - challenge accepted?
Dominic Docter (2015)
Zinc Oxide Nanoparticles Promote the Aggregation of Concanavalin A
Taqi Ahmed Khan (2012)
Improving the sensitivity of immunoassays by reducing non-specific binding of poly(acrylic acid) coated upconverting nanoparticles by adding free poly(acrylic acid)
Satu Lahtinen (2018)
See more
Semantic Scholar Logo Some data provided by SemanticScholar