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Flow Cytometric Analysis Of The Binding Of Eleven Lectins To Human T- And B-cells And To Human T- And B-cell Lines.

J Malin-Berdel, Gilbert Valet, E. Thiel, J. A. Forrester, Lutz Guertler
Published 1984 · Medicine, Biology
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The relative surface binding of 11 lectins to human peripheral blood T- and B-lymphocytes, to Molt-4 and JM T-cell lines, and to 6410 and NC37 B-cell lines was determined by flow cytometry. The lectins from Lens culinaris (LCA), Ricinus communis (RCA), Arachis hypogaea (PNA), Abrus precatorius (APA), Ulex europaeus (UEA-F), Sarothamnus scoparius (SAS-F), Helix pomatia (HPA), Phaseolus coccineus (L-PHA), Glycine max (SBA), and Triticum vulgare (WGA) were fluoresceinated and incubated with living, formaldehyde-fixed, or neuraminidase-treated cells. Except LCA, which preferentially bound to the two B-cell lines tested in this study, none of the other lectins exhibited selective binding to the undifferentiated cells of the cell lines. The T-cell lines and, in part, the peripheral blood T-cells bound less WGA, APA, LCA, and L-PHA than the B-cell lines and the peripheral blood B-cells. Binding of PNA was found only after neuraminidase treatment of the cells; the binding of PNA, HPA, and UEA-F after neuraminidase treatment was higher for the T-cells than the B-cells from peripheral blood. No significant differences were detected between both cell types for RCA, ConA, SBA, and SAS-F.
This paper references
10.1021/bi00698a029
Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma.
Garth L. Nicolson (1974)
10.1042/bj1290847
The purification and characterization of wheat-germ agglutinin.
D. Levine (1972)
The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea).
R. Lotan (1975)
10.1016/S0065-2318(08)60220-6
The lectins: carbohydrate-binding proteins of plants and animals.
Irwin J. Goldstein (1978)
Studies on a phytohemagglutinin from the lentil. II. Multiple forms of Lens culinaris hemagglutinin.
Irmgard K. Howard (1971)
10.1016/0005-2795(67)90404-7
Protein-carbohydrate interaction. VI. Isolation of concanavalin A by specific adsorption on cross-linked dextran gels.
Bharat B. Agrawal (1967)
10.1016/0304-4165(78)90333-1
The fucosyl specific lectins of Ulex europaeus and Sarothamnus scoparius. Biochemical characteristics and binding properties to human B-lymphocytes.
Lutz G. Gürtler (1978)
Effect of conjugation of Con- canavalin A with fluorescein isothiocyanate on its mitogenic ef- fect
PH Pinkerton (1983)
10.1038/newbio237189a0
Occurrence of Epstein-Barr virus genomes in human lymphoblastoid cell lines.
H zur Hansen (1972)
10.1073/pnas.76.1.447
Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells.
Yair Reisner (1979)
Computer analysis of two parameter histograms of rat bone marrow cells: Cell volume to DNA relationship measured by means of flow cytometry
G Valet (1978)
10.1177/27.1.86564
Flow cytometry as a new method for the measurement of electrophoretic mobility of erythrocytes using membrane charge staining by fluoresceinated polycations.
Günter K Valet (1979)
10.1042/bj2080069
Flow cytometric analysis of lectin binding to human peripheral blood lymphocytes.
George R.P. Blackledge (1982)
10.1002/ijc.2910170107
Different surface glycoprotein patterns on human T-, B- and leukemic-lymphocytes.
Leif C Andersson (1976)
Epstein-Barr virus-induced increase in the concanavalin-A receptor density of established EBV-negative lymphoma lines in vitro.
Wolfgang Siegert (1980)
10.1016/0304-4165(72)90304-2
Binding of soybean agglutinin by normal and trypsin-treated red blood cells.
J. A. Gordon (1972)
A model of lymphoid-myeloid cell differentiation based on the study of marker profiles of 50 human leukemia-lymphoma cell lines
J Minowada (1980)
10.1073/pnas.76.8.4061
Separation of functional subsets of human T cells by a monoclonal antibody.
Ellis L. Reinherz (1979)
10.1177/24.1.1254918
Basic principles of electrical sizing of cells and particles and their realization in the new instrument "Metricell".
Volker Kachel (1976)
10.5694/j.1326-5377.1962.tb20820.x
Fluorescent protein tracing
Richard C. Nairn (1969)
10.1016/0022-1759(74)90003-9
An improved rosetting assay for detection of human T lymphocytes.
Manuel E. Kaplan (1974)
Diagnosis and treatment of human leukemias and lymphomas utilizing monoclonal antibodies.
Lee M. Nadler (1981)
Lectin receptors on human blood and bone marrow cells and their use in cell separation.
Nicos Anthony Nicola (1980)
10.1002/chin.197851370
THE LECTINS: CARBOHYDRATE-BINDING PROTEINS OF PLANTS AND ANIMALS
Irwin J. Goldstein (1978)
Partial isolation and characterization of Gp 220, the Ulex europaeus binding glycoprotein of the B-lymphocyte plasma membrane.
Lutz G. Gürtler (1981)
10.1093/jnci/64.5.1241
Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines.
Christopher L. Reading (1980)
Lectins. In: International Review of Cytol
JC Brown (1978)
10.1111/j.1365-3083.1978.tb00443.x
Receptors for Helix pomatia A haemagglutinin (HP) on a subpopulation of human B cells.
Ulla B Hellström (1978)
10.1016/0003-9861(76)90357-X
Isolation, purification, and some properties of a lectin and abrin from Abrus precatorius linn.
J. Albert Roy (1976)
10.1515/bchm2.1979.360.1.421
The lectin-binding sites of the erythrocyte membrane components of horse, swine and sheep. Characterization by their molecular weights.
Lutz G. Gürtler (1979)
10.1038/bjc.1981.149
Binding of peanut lectin to germinal-centre cells: a marker for B-cell subsets of follicular lymphoma?
Michelle L. Rose (1981)
10.1177/25.7.330731
Fluvo-metricell, a combined cell volume and cell fluorescence analyzer.
Volker Kachel (1977)
10.1007/BF01028359
Binding of lectins to leukemic cell lines
Hans U. Schwenk (1980)
10.1002/eji.1830081102
Helix pomatia A hemagglutinin: selectivity of binding to lymphocyte surface glycoproteins on T cells and certain B cells.
Bengt Ingemar Axelsson (1978)



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