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Quantification Of Mitochondrial Reactive Oxygen Species In Living Cells By Using Multi‐laser Polychromatic Flow Cytometry

S. de Biasi, L. Gibellini, E. Bianchini, M. Nasi, M. Pinti, S. Salvioli, A. Cossarizza
Published 2016 · Biology, Medicine

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Reactive oxygen species (ROS) are constantly produced in cells, mainly by mitochondria, as a consequence of aerobic respiration. Most ROS derive from superoxide, which is rapidly converted to hydrogen peroxide. ROS are involved in the regulation of several physiological and pathological processes, and the possibility to measure them simultaneously is needed, when the redox status of the cells is modified by experimental/biological conditions. Flow cytometry is the main technology that generates multiple information at the single cell level in a high‐throughput manner, and gives rapid and quantitative measurements of different ROS with high sensitivity and reproducibility. Here, we describe a novel approach to detect simultaneously mitochondrial hydrogen peroxide and mitochondrial superoxide in living cells. The staining has been performed by using the fluorescent dyes MitoSOX Red Mitochondrial Superoxide Indicator, Mitochondria Peroxy Yellow 1, Annexin‐V Pacific Blue conjugate, TO‐PRO‐3 iodide, anti‐CD4‐APC‐Cy7 and ‐CD8‐Pacific Orange mAbs. We used this approach to quantify mitochondrial ROS in CD4+ and CD8+ T cells form patients affected by Down syndrome and age‐ and sex‐matched healthy donors. © 2016 International Society for Advancement of Cytometry
This paper references
10.1002/0471142956.cy0732s72
Uncompensated Polychromatic Analysis of Mitochondrial Membrane Potential Using JC‐1 and Multilaser Excitation
S. de Biasi (2015)
10.1021/ja802355u
A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells.
Bryan C. Dickinson (2008)
10.1016/j.febslet.2006.12.058
Mitochondrial alterations and tendency to apoptosis in peripheral blood cells from children with Down syndrome
E. Roat (2007)
10.1038/nprot.2007.327
Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy
Partha Mukhopadhyay (2007)
10.18632/oncotarget.4510
Inhibition of Lon protease by triterpenoids alters mitochondria and is associated to cell death in human cancer cells
L. Gibellini (2015)
10.1016/j.canep.2014.07.006
Epidemiology of childhood leukemia in the presence and absence of Down syndrome.
G. Mezei (2014)
10.1038/nprot.2013.064
Preparation and use of MitoPY1 for imaging hydrogen peroxide in mitochondria of live cells
Bryan C. Dickinson (2013)
10.1016/j.cell.2005.05.019
Apoptotic Pathways: Ten Minutes to Dead
D. R. Green (2005)
10.1016/j.cell.2016.07.002
Mitochondria and Cancer
Sejal Vyas (2016)
10.1089/ars.2012.4886
Methods for detection of mitochondrial and cellular reactive oxygen species.
S. Dikalov (2014)
10.1111/bjh.13096
Haematopoietic development and leukaemia in Down syndrome
I. Roberts (2014)
10.1186/s12916-015-0515-0
Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments
S. de Biasi (2015)
10.1016/j.cell.2015.10.001
Mitochondrial ROS Signaling in Organismal Homeostasis
G. Shadel (2015)
10.1002/cyto.a.22019
Immunological advantages of everolimus versus cyclosporin A in liver‐transplanted recipients, as revealed by polychromatic flow cytometry
E. Roat (2012)
10.1016/j.cub.2014.03.034
ROS Function in Redox Signaling and Oxidative Stress
Michael Schieber (2014)
10.1093/ecam/neq053
Quercetin and Cancer Chemoprevention
L. Gibellini (2011)
10.1038/nprot.2009.189
Simultaneous analysis of reactive oxygen species and reduced glutathione content in living cells by polychromatic flow cytometry
A. Cossarizza (2009)
10.1038/cddis.2016.105
ROS homeostasis and metabolism: a dangerous liason in cancer cells
E. Panieri (2016)
10.3390/cancers2021288
Interfering with ROS Metabolism in Cancer Cells: The Potential Role of Quercetin
L. Gibellini (2010)
10.1016/j.cmet.2012.12.005
Adaptive downregulation of mitochondrial function in down syndrome.
P. Helguera (2013)
10.1042/BJ20081386
How mitochondria produce reactive oxygen species
M. Murphy (2009)
10.1097/QAD.0b013e328359b8be
The protease inhibitor atazanavir triggers autophagy and mitophagy in human preadipocytes
L. Gibellini (2012)
10.1002/jemt.22207
Imaging of reactive oxygen species burst from mitochondria using laser scanning confocal microscopy
Jingman Xu (2013)



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