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Active Repression Of Transcription By The Engrailed Homeodomain Protein.

J. Jaynes, P. O'Farrell
Published 1991 · Medicine, Biology

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The Drosophila engrailed gene product (En) is a homeodomain‐containing protein that contributes to segmental patterning. In transfection assays it acts as a transcriptional repressor. We show that En is an active repressor, blocking activation by mammalian and yeast activators that bind to sites some distance away from those bound by En. Active repression is distinct from the effects of passive homeodomain‐containing proteins, which repress when competing with activators for binding sites and activate when competing with En. Active repression activity maps outside the En homeodomain, and this activity can be transferred to a heterologous DNA binding domain.



This paper is referenced by
10.1007/s004270100135
Drosophila SAP18, a member of the Sin3/Rpd3 histone deacetylase complex, interacts with Bicoid and inhibits its activity
W. Zhu (2001)
Inhibition of Apoptotic Cell Death Required for Cellular Proliferation and Factor Complex-Regulated Gene Activity Is Ternary Complex Factor-Serum Response
Andrew D. Sharrocks (2004)
10.1016/S1534-5807(02)00172-7
Smad10 is required for formation of the frog nervous system.
J. LeSueur (2002)
10.1242/dev.00286
Requirements for transcriptional repression and activation by Engrailed in Drosophila embryos
C. Alexandre (2003)
10.1074/jbc.274.21.14678
Cloning and Characterization of a Novel Zinc Finger Transcriptional Repressor
G. He (1999)
10.1016/B978-012505255-9/50022-5
Maternal Signaling Pathways and the Regulation of Cell Fate
D. Kessler (1999)
10.1007/PL00008239
Altered expression of insulin-like growth factor -1 and insulin like growth factor binding proteins-2 and 5 in the mouse mutant Hypodactyly (Hd) correlates with sites of apoptotic activity
G. Allan (2000)
10.1387/ijdb.8104460
Genomic sequence and embryonic expression of the zebrafish homeobox gene hox-3.4.
J. Ericson (1993)
10.1073/PNAS.94.20.10885
Oncogenic transformation induced by the Qin protein is correlated with transcriptional repression.
J. Li (1997)
10.1007/s00427-002-0216-5
Heterospecific transgenesis in Drosophila suggests that engrailed.a is regulated by POU proteins in the crustacean Sacculina carcini
J. Gibert (2002)
10.1006/DBIO.2002.0705
The competence of marginal zone cells to become Spemann's organizer is controlled by Xcad2.
Vered Levy (2002)
10.1182/BLOOD-2002-12-3809
Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos.
N. Minegishi (2003)
10.1128/MCB.16.11.6263
The Gfi-1 proto-oncoprotein contains a novel transcriptional repressor domain, SNAG, and inhibits G1 arrest induced by interleukin-2 withdrawal.
H. L. Grimes (1996)
Inserting the Ftz homeodomain into engrailed creates a dominant transcriptional repressor that specifically turns off Ftz target genes in vivo.
A. John (1995)
10.1242/dev.01370
Essential role of Gata transcription factors in sympathetic neuron development
K. Tsarovina (2004)
10.1242/dev.00474
Tbx5 and Tbx4 trigger limb initiation through activation of the Wnt/Fgf signaling cascade
J. Takeuchi (2003)
Role of Xrx1 in Xenopus eye and anterior brain development.
M. Andreazzoli (1999)
interacts directly with TATA-binding protein . The transcriptional repressor even-skipped
Li Chi (1995)
10.1016/S0925-4773(02)00137-5
L-Maf, a downstream target of Pax6, is essential for chick lens development
H. Reza (2002)
10.1093/MOLBEV/MSG140
The evolution of transcriptional regulation in eukaryotes.
G. Wray (2003)
10.1242/dev.012856
Mechanism of asymmetric ovarian development in chick embryos
Y. Ishimaru (2008)
10.1002/dvg.20403
Evolutionary conservation of vertebrate notochord genes in the ascidian Ciona intestinalis.
J. Kugler (2008)
Groucho augments the repression of multiple Even skipped target genes in establishing parasegment boundaries.
M. Kobayashi (2001)
10.1128/MCB.24.23.10340-10351.2004
Ternary Complex Factor-Serum Response Factor Complex-Regulated Gene Activity Is Required for Cellular Proliferation and Inhibition of Apoptotic Cell Death
E. Vickers (2004)
10.1016/J.YDBIO.2005.12.037
Mxi1 is essential for neurogenesis in Xenopus and acts by bridging the pan-neural and proneural genes.
Tiemo J Klisch (2006)
10.1016/J.EXPHEM.2006.09.004
Altered regulation of beta-like globin genes by a redesigned erythroid transcription factor.
D. Manwani (2007)
10.1016/j.ydbio.2009.02.022
Trithorax, Hox, and TALE-class homeodomain proteins ensure cell survival through repression of the BH3-only gene egl-1.
Malia B. Potts (2009)
10.1016/S0092-8674(00)80770-9
Reciprocal Interactions of Pit1 and GATA2 Mediate Signaling Gradient–Induced Determination of Pituitary Cell Types
J. Dasen (1999)
10.1016/S0925-4773(02)00148-X
Chick homeobox gene cbx and its role in retinal development
Chuan-Ming Li (2002)
10.1046/J.1365-313X.2003.01815.X
Generation of dominant-negative effects on the heat shock response in Arabidopsis thaliana by transgenic expression of a chimaeric HSF1 protein fusion construct.
M. Wunderlich (2003)
10.1074/jbc.M112.356394
Caudal-related Homeobox (Cdx) Protein-dependent Integration of Canonical Wnt Signaling on Paired-box 3 (Pax3) Neural Crest Enhancer*
O. S. Ferras (2012)
10.1242/dev.071720
The lineage-specific gene ponzr1 is essential for zebrafish pronephric and pharyngeal arch development
V. M. Bedell (2012)
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