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Thermoreversible Hydrogel Scaffolds For Articular Cartilage Engineering.

John P. Fisher, Seongbong Jo, Antonios G. Mikos, A. Hari Reddi
Published 2004 · Materials Science, Medicine
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Articular cartilage has limited potential for repair. Current clinical treatments for articular cartilage damage often result in fibrocartilage and are associated with joint pain and stiffness. To address these concerns, researchers have turned to the engineering of cartilage grafts. Tissue engineering, an emerging field for the functional restoration of articular cartilage and other tissues, is based on the utilization of morphogens, scaffolds, and responding progenitor/stem cells. Because articular cartilage is a water-laden tissue and contains within its matrix hydrophilic proteoglycans, an engineered cartilage graft may be based on synthetic hydrogels to mimic these properties. To this end, we have developed a polymer system based on the hydrophilic copolymer poly(propylene fumarate-co-ethylene glycol) [P(PF-co-EG)]. Solutions of this polymer are liquid below 25 degrees C and gel above 35 degrees C, allowing an aqueous solution containing cells at room temperature to form a hydrogel with encapsulated cells at physiological body temperature. The objective of this work was to determine the effects of the hydrogel components on the phenotype of encapsulated chondrocytes. Bovine articular chondrocytes were used as an experimental model. Results demonstrated that the components required for hydrogel fabrication did not significantly reduce the proteoglycan synthesis of chondrocytes, a phenotypic marker of chondrocyte function. In addition, chondrocyte viability, proteoglycan synthesis, and type II collagen synthesis within P(PF-co-EG) hydrogels were investigated. The addition of bone morphogenetic protein-7 increased chondrocyte proliferation with the P(PF-co-EG) hydrogels, but did not increase proteoglycan synthesis by the chondrocytes. These results indicate that the temperature-responsive P(PF-co-EG) hydrogels are suitable for chondrocyte delivery for articular cartilage repair.
This paper references
10.1097/00003086-200209000-00004
Articular cartilage injuries.
Joseph A. Buckwalter (2002)
10.1016/j.orthres.2003.11.006
Chondrocyte viability in press-fit cryopreserved osteochondral allografts.
Madhura Dilip Gole (2004)
Natural bovine osteogenin and recombinant human bone morphogenetic protein-2B are equipotent in the maintenance of proteoglycans in bovine articular cartilage explant cultures.
Frank P. Luyten (1992)
10.1089/107632702320934074
Collagen fibrillogenesis by chondrocytes in alginate.
Marcy Wong (2002)
10.1016/0092-8674(82)90027-7
Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels
Paul D. Benya (1982)
10.1111/j.1749-6632.2002.tb03062.x
Biological response of chondrocytes to hydrogels.
Jennifer H Elisseeff (2002)
10.2106/00004623-199708000-00003
Stimulation of Proteoglycan Synthesis in Explants of Porcine Articular Cartilage by Recombinant Osteogenic Protein-1 (Bone Morphogenetic Protein-7)*
Steven A. Lietman (1997)
10.1021/bm010158r
Synthesis of in situ cross-linkable macroporous biodegradable poly(propylene fumarate-co-ethylene glycol) hydrogels.
Esfandiar Behravesh (2002)
10.2106/00004623-200312000-00045
Autologous Chondrocyte Implantation and Osteochondral Cylinder Transplantation in Cartilage Repair of the Knee Joint
Uwe Horas (2003)
10.2307/1270298
Applied Statistics for Engineers and Physical Scientists
Eric R. Ziegel (1991)
10.1038/nbt0398-247
Role of morphogenetic proteins in skeletal tissue engineering and regeneration
A. Hari Reddi (1998)
10.3109/03008208909043901
Culture and growth characteristics of chondrocytes encapsulated in alginate beads.
J F Guo (1989)
10.1038/nbt864
The application of bone morphogenetic proteins to dental tissue engineering
Misako Nakashima (2003)
10.1097/00003086-199210000-00003
Intact articular cartilage cryopreservation. In vivo evaluation.
Fernando Marco (1992)
10.1097/00003086-199406000-00004
Cryopreservation of articular cartilage. Ultrastructural observations and long-term results of experimental distal femoral transplantation.
Theodore I. Malinin (1994)
10.1053/joca.1997.0092
Transplantation of allograft chondrocytes embedded in agarose gel into cartilage defects of rabbits.
B. Rahfoth (1998)
10.3181/00379727-172-41533
Cell Shape and Phenotypic Expression in Chondrocytes
Julie Glowacki (1983)
10.1016/S0142-9612(98)00017-9
The importance of physicochemical swelling in cartilage illustrated with a model hydrogel system.
Neil D. Broom (1998)
10.2307/2984653
Applied Linear Statistical Models
John Neter (1974)
10.1097/00003086-200110001-00024
Biodegradable Polymer Scaffolds for Cartilage Tissue Engineering
Lichun Lu (2001)
10.1002/1097-4636(200111)57:2<268::AID-JBM1167>3.0.CO;2-L
Sodium alginate sponges with or without sodium hyaluronate: in vitro engineering of cartilage.
G Miralles (2001)
10.1021/bm010137x
Synthesis and characterization of triblock copolymers of methoxy poly(ethylene glycol) and poly(propylene fumarate).
Esfandiar Behravesh (2002)



This paper is referenced by
10.1016/j.biomaterials.2009.07.062
The use of green fluorescence gene (GFP)-modified rabbit mesenchymal stem cells (rMSCs) co-cultured with chondrocytes in hydrogel constructs to reveal the chondrogenesis of MSCs.
Han Na Yang (2009)
10.1042/BST0381072
Developments in three-dimensional cell culture technology aimed at improving the accuracy of in vitro analyses.
Daniel J. Maltman (2010)
10.1038/nprot.2012.055
Synthesis of oligo(poly(ethylene glycol) fumarate)
Lucas A. Kinard (2012)
10.1007/978-1-84628-366-6_4
Biodegradable Orthopedic Implants
Hansoo Park (2007)
Electrospun nanofiber meshes for the functional repair of bone defects
Yash M. Kolambkar (2009)
10.1002/term.1683
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
Phase transfer cell culture system for self-forming cartilage tissue regeneration
Kai Su (2011)
Thermoresponsive hydrogels in biomedical applications-a review
Leda Klouda (2011)
10.1002/bip.21715
Invited review current progress and limitations of spider silk for biomedical applications.
Mona Widhe (2012)
10.1007/978-1-84628-366-6
Engineering of functional skeletal tissues
Felix Bronner (2007)
10.2174/1381612821666150115150712
Bioactive hydrogel scaffolds - advances in cartilage regeneration through controlled drug delivery.
Roberta Censi (2015)
10.1007/s13770-014-9044-8
Tissue engineering of articular cartilage: From bench to bed-side
Rozlin Abdul Rahman (2014)
10.22203/ECM.V021A24
Osteogenic efficiency of in situ gelling poloxamine systems with and without bone morphogenetic protein-2.
Ana Rey-Rico (2011)
Abstract. This study explored the feasibility of inducing the differentiation of BMSCs into chondrocytes through co-culture with chondrocytes in hydrogel constructs (Pluronic F-127 gel) in vivo for the repair of goat mandibular condylar cartilage
Hao Sun (2018)
10.1021/am506026e
Hairy polyelectrolyte brushes-grafted thermosensitive microgels as artificial synovial fluid for simultaneous biomimetic lubrication and arthritis treatment.
Guoqiang Liu (2014)
10.1002/9781119044901.CH8
Polymer Modifications and Recent Technological Advances toward Live Cell Encapsulation and Delivery
Paulomi Ghosh (2015)
10.1007/978-0-387-74660-9_4
Biomimetic and Bio-responsive Materials in Regenerative Medicine
Jacob Freas Pollock (2009)
10.1007/s12539-012-0236-4
An agent-based model approach to multi-phase life-cycle for contact inhibited, anchorage dependent cells
Ross. D. Hoehn (2012)
10.1007/s10856-011-4396-2
A hydrophobically-modified alginate gel system: utility in the repair of articular cartilage defects
Mohammad Kazem Ghahramanpoor (2011)
Mesenchymal stem cells as trophic mediators of neural differentiation
Steven A. Hardy (2010)
10.1016/j.addr.2007.03.019
Matrices and scaffolds for drug delivery in dental, oral and craniofacial tissue engineering.
Eduardo K. Moioli (2007)
10.1016/j.jneumeth.2005.06.029
Activation of membrane receptors by neurotransmitter released from temperature-sensitive hydrogels
Niraj J Muni (2006)
10.3390/ma8041778
Bone Regeneration Using Bone Morphogenetic Proteins and Various Biomaterial Carriers
Zeeshan Sheikh (2015)
10.1533/9780857091376.3.354
Biocompatibility of injectable materials
Scott A Guelcher (2011)
10.1002/jbm.a.32341
Chondrogenesis of human mesenchymal stem cells encapsulated in a hydrogel construct: neocartilage formation in animal models as both mice and rabbits.
Ji sun Park (2009)
10.1002/jbm.a.31585
In vitro and in vivo study to the biocompatibility and biodegradation of hydroxyapatite/poly(vinyl alcohol)/gelatin composite.
Mingbo Wang (2008)
10.1002/9780470891315.CH6
Injectable Hydrogels as Biomaterials
Lakshmi Nair (2010)
Assessment of Biologic Resurfacing of Femoral Head in Dogs through Membranous Layer of Fetal Sheep Skull
Reza Khandanlou (2011)
10.1002/jbm.a.33140
Thermoresponsive poly(N-isopropylacrylamide)-g-methylcellulose hydrogel as a three-dimensional extracellular matrix for cartilage-engineered applications.
Helena Sá-Lima (2011)
10.4172/2155-9538.S2-002
Organotypic Cultures of Hepg2 Cells for In Vitro Toxicity Studies
Daniel J. Müller (2011)
Biodegradable Hydrogel Composites for Growth Factor and Stem Cell Delivery in Osteochondral Tissue Engineering
Steven Lu (2016)
Development and Characterization of Photocrosslinkable Hyaluronic Acid Hydrogels for Cartilage Regeneration
Cindy Mann Yien Chung (2009)
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