Online citations, reference lists, and bibliographies.

Alginate/lactose-modified Chitosan Hydrogels: A Bioactive Biomaterial For Chondrocyte Encapsulation.

Eleonora Marsich, Massimiliano Borgogna, Ivan Donati, Pamela Mozetic, Berit Løkensgard Strand, Santiago Gomez Salvador, Franco Vittur, Sergio Paoletti
Published 2008 · Materials Science, Chemistry, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
A new bioactive scaffold was prepared from a binary polysaccharide mixture composed of a polyanion (alginate) and a polycation (a lactose-modified chitosan, chitlac). Its potential use for articular chondrocytes encapsulation and cartilage reconstructive surgery applications has been studied. The hydrogel combines the ability of alginate to act as a 3D supporting structure with the capability of the second component (chitlac) to provide interactions with porcine articular chondrocytes. Physico-chemical characterization of the scaffold was accomplished by gel kinetics and compression measurements and demonstrated that alginate-chitlac mixture (AC-mixture) hydrogels exhibit better mechanical properties when compared with sole alginate hydrogels. Furthermore, biochemical and biological studies showed that these 3D scaffolds are able to maintain chondrocyte phenotype and particularly to significantly stimulate and promote chondrocyte growth and proliferation. In conclusion, the present study can be considered as a first step towards an engineered, biologically active scaffold for chondrocyte in vitro cultivation, expansion, and cell delivery.



This paper is referenced by
10.1201/B19751-15
Alginate Hydrogels: Properties and Applications
Ivan Donati (2015)
10.1016/j.ijbiomac.2019.09.215
Comparison of polysaccharides in articular cartilage regeneration associated with chondrogenic and autophagy-related gene expression.
Dong Nyoung Heo (2019)
10.1021/bm5014124
Use of methacrylate-modified chitosan to increase the durability of dentine bonding systems.
Marina Diolosà (2014)
10.1021/jp4067263
Insight into the molecular properties of Chitlac, a chitosan derivative for tissue engineering.
Nicola D’Amelio (2013)
A biocompatible, heparin-binding polycation for the controlled delivery of growth factors
Blaine Zern (2009)
10.1016/j.actbio.2018.03.041
Exploiting natural polysaccharides to enhance in vitro bio-constructs of primary neurons and progenitor cells.
Manuela Medelin (2018)
10.1039/C3SM51375K
Kinetics of chitosan hydrogel formation in high internal phase oil-in-water emulsions (HIPEs) using viscoelastic measurements
Jonathan Miras (2013)
10.1371/journal.pone.0162853
3D Cell Culture in a Self-Assembled Nanofiber Environment
Yi Wen Chai (2016)
10.1002/MACP.201300057
Polysaccharide-Based Polyanion–Polycation–Polyanion Ternary Systems in the Concentrated Regime and Hydrogel Form
Eleonora Marsich (2013)
10.1002/jor.24259
Effects of intra‐articular hyaluronic acid associated to Chitlac (arty‐duo®) in a rat knee osteoarthritis model
Francesca Salamanna (2019)
10.1016/j.carbpol.2016.03.088
Hyaluronan delivery by polymer demixing in polysaccharide-based hydrogels and membranes for biomedical applications.
Andrea Travan (2016)
10.1039/c1cs15203c
Injectable and biodegradable hydrogels: gelation, biodegradation and biomedical applications.
Yulin Li (2012)
10.5124/jkma.2009.52.11.1077
Cartilage Repair Using Mesenchymal Stem Cells
Byoung Hyun Min (2009)
10.1016/j.ijbiomac.2016.11.017
On the demixing of hyaluronan and alginate in the gel state.
Francesca Scognamiglio (2017)
10.1016/B978-1-4557-3146-6.00010-6
Cell Microencapsulation for Tissue Engineering and Regenerative Medicine
Jian Du (2014)
10.1002/term.2365
Antibacterial-nanocomposite bone filler based on silver nanoparticles and polysaccharides.
Davide Porrelli (2018)
10.1016/j.jconrel.2013.04.015
Therapeutic cell encapsulation: ten steps towards clinical translation.
Edorta Santos (2013)
10.1159/000209230
Genipin Cross-Linked Fibrin Hydrogels for in vitro Human Articular Cartilage Tissue-Engineered Regeneration
Emma V. Dare (2009)
10.1371/journal.pone.0159342
Sustained Delivery of Bioactive GDNF from Collagen and Alginate-Based Cell-Encapsulating Gel Promoted Photoreceptor Survival in an Inherited Retinal Degeneration Model
Francisca Siu Yin Wong (2016)
10.1016/B978-0-12-409547-2.12487-4
Self-Assembly in Aqueous Media
Frank Biedermann (2017)
10.3390/gels4030067
Concepts for Developing Physical Gels of Chitosan and of Chitosan Derivatives
Pasquale Sacco (2018)
10.1201/B14540-4
Microcapsules for Cell Transplantation: Design, Preparation and Application
Guojun Lv (2013)
10.1002/JCTB.4505
Polymers for medical and tissue engineering applications
Deniz Ozdil (2014)
10.1007/978-3-319-57153-9_7
Encapsulation Approaches to Cell Therapy
Paul De Vos (2017)
10.1016/B978-0-12-811942-6.00009-1
Nano-delivery Systems for Nutraceutical Application
Khairul Anwar Ishak (2017)
10.1016/j.ijbiomac.2017.08.059
Mimicking mechanical response of natural tissues. Strain hardening induced by transient reticulation in lactose-modified chitosan (chitlac).
Michela Cok (2018)
10.1016/j.carbpol.2017.06.097
Highly monodisperse colloidal coacervates based on a bioactive lactose-modified chitosan: From synthesis to characterization.
Franco Furlani (2017)
10.1007/s00249-011-0753-9
Biophysical functionality in polysaccharides: from Lego-blocks to nano-particles
Attilio Cesàro (2011)
10.1533/9781845699802.2.236
Alginate-based drug delivery devices
Lisbeth Grøndahl (2010)
Enhancement of Chondrogenesis via Co-culture of Bovine Chondrocytes with Demineralized Bone Matrix in Chitosan-alginate Beads
Bc Sutradhar (2013)
10.1016/j.jconrel.2010.09.023
Cell microcarriers and microcapsules of stimuli-responsive polymers.
Amanda K. Andriola Silva Brun-Graeppi (2011)
10.1016/B978-1-78242-452-9.00012-1
Biomimetic nanocomposite hydrogels for cartilage regeneration
Nathan J. Castro (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar