Online citations, reference lists, and bibliographies.

Genipin-cross-linked Collagen/chitosan Biomimetic Scaffolds For Articular Cartilage Tissue Engineering Applications.

L. Yan, Ying-jun Wang, Li Ren, G. Wu, S. Caridade, Jia-Bing Fan, L. Wang, P. Ji, J. Oliveira, J. T. Oliveira, J. Mano, R. Reis
Published 2010 · Materials Science, Medicine

Cite This
Download PDF
Analyze on Scholarcy
In this study, genipin-cross-linked collagen/chitosan biodegradable porous scaffolds were prepared for articular cartilage regeneration. The influence of chitosan amount and genipin concentration on the scaffolds physicochemical properties was evaluated. The morphologies of the scaffolds were characterized by scanning electron microscope (SEM) and cross-linking degree was investigated by ninhydrin assay. Additionally, the mechanical properties of the scaffolds were assessed under dynamic compression. To study the swelling ratio and the biostability of the collagen/chitosan scaffold, in vitro tests were also carried out by immersion of the scaffolds in PBS solution or digestion in collagenase, respectively. The results showed that the morphologies of the scaffolds underwent a fiber-like to a sheet-like structural transition by increasing chitosan amount. Genipin cross-linking remarkably changed the morphologies and pore sizes of the scaffolds when chitosan amount was less than 25%. Either by increasing the chitosan ratio or performing cross-linking treatment, the swelling ratio of the scaffolds can be tailored. The ninhydrin assay demonstrated that the addition of chitosan could obviously increase the cross-linking efficiency. The degradation studies indicated that genipin cross-linking can effectively enhance the biostability of the scaffolds. The biocompatibility of the scaffolds was evaluated by culturing rabbit chondrocytes in vitro. This study demonstrated that a good viability of the chondrocytes seeded on the scaffold was achieved. The SEM analysis has revealed that the chondrocytes adhered well to the surface of the scaffolds and contacted each other. These results suggest that the genipin-cross-linked collagen/chitosan matrix may be a promising formulation for articular cartilage scaffolding.
This paper references
Effect of solvent-dependent viscoelastic properties of chitosan membranes on the permeation of 2-phenylethanol
S. Caridade (2009)
Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering.
L. Ma (2003)
Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking.
S. Park (2002)
Colla - gen / chitosan porous scaffolds with improved biostability for skin tissue engineering
L Ma (2003)
Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells.
J. Oliveira (2006)
Tissue engineering : Frontiers in biotechnology
R. Langer (1993)
Osteochondral defects: present situation and tissue engineering approaches.
J. Mano (2007)
The effect of cross-linking of chitosan microspheres with genipin on protein release
Youling Yuan (2007)
Mechanical compression alters gene expression and extracellular matrix synthesis by chondrocytes cultured in collagen I gels.
C. J. Hunter (2002)
Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin-polyurethane composites is modulated by frequency and amplitude of dynamic compression and shear stress.
Z. Li (2010)
Current concepts in the treatment of articular cartilage defects.
T. Minas (1997)
Selective enhancement of collagenase-mediated cleavage of resident type II collagen in cultured osteoarthritic cartilage and arrest with a synthetic inhibitor that spares collagenase 1 (matrix metalloproteinase 1).
L. Dahlberg (2000)
Preparation and characterisation in simulated body conditions of glutaraldehyde crosslinked chitosan membranes
R. M. Silva (2004)
Physical crosslinking of collagen fibers: comparison of ultraviolet irradiation and dehydrothermal treatment.
K. Weadock (1995)
Effects of the controlled-released TGF-beta 1 from chitosan microspheres on chondrocytes cultured in a collagen/chitosan/glycosaminoglycan scaffold.
J. Lee (2004)
Antigenicity and immunogenicity of collagen.
A. K. Lynn (2004)
Novel genipin-cross-linked chitosan/silk fibroin sponges for cartilage engineering strategies.
Simone S Silva (2008)
Biodegradable Systems in Tissue Engineering and Regenerative Medicine
R. Reis (2004)
Molecular interactions in collagen and chitosan blends.
A. Sionkowska (2004)
Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits.
X. X. Shao (2006)
Viscoelastic properties of chitosan with different hydration degrees as studied by dynamic mechanical analysis.
J. Mano (2008)
tosan scaffolds : Interconnective pore size and cartilage engineer
DJ Griffon (2006)
Feasibility of polysaccharide hybrid materials for scaffolds in cartilage tissue engineering: evaluation of chondrocyte adhesion to polyion complex fibers prepared from alginate and chitosan.
N. Iwasaki (2004)
Repair of large full-thickness articular cartilage defects with allograft articular chondrocytes embedded in a collagen gel.
S. Wakitani (1998)
Dynamic Mechanical Analysis in Polymers for Medical Applications
J. F. Mano (2002)
Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate.
J. S. Pieper (1999)
Cartilage regeneration using mesenchymal stem cells and a PLGA-gelatin/chondroitin/hyaluronate hybrid scaffold.
Hongbin Fan (2006)
Thermal dehydration treatment and glutaraldehyde cross-linking to increase the biostability of collagen–chitosan porous scaffolds used as dermal equivalent
Lie (2003)
Chitosan: a versatile biopolymer for orthopaedic tissue-engineering.
A. Di Martino (2005)
Viscoelastic Behavior of Poly(methyl methacrylate) Networks with Different Cross-Linking Degrees
N. Alves (2004)
Mechanical properties of a porcine aortic valve fixed with a naturally occurring crosslinking agent.
H. Sung (1999)
Genipin-crosslinked chitosan/gelatin blends for biomedical applications
V. Chiono (2008)
Chitosan particles agglomerated scaffolds for cartilage and osteochondral tissue engineering approaches with adipose tissue derived stem cells
P. B. Malafaya (2006)
Synthesis and characterization of a novel chitosan‐based network prepared using naturally occurring crosslinker
F. L. Mi (2000)
A cartilage tissue engineering approach combining starch-polycaprolactone fibre mesh scaffolds with bovine articular chondrocytes
J. T. Oliveira (2007)
Chitosan scaffolds: interconnective pore size and cartilage engineering.
D. Griffon (2006)
Mechanical Characterization of Biomaterials
J. F. Mano (2004)
Biomedical applications of collagen.
C. H. Lee (2001)
The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid.
J. F. Wößner (1961)
Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes.
A. Marsano (2007)
Morphology, mechanical characterization and in vivo neo-vascularization of chitosan particle aggregated scaffolds architectures.
Patrícia B. Malafaya (2008)
Genipin-crosslinked gelatin microspheres as a drug carrier for intramuscular administration: in vitro and in vivo studies.
H. Liang (2003)
Tissue engineering.
R. Langer (1993)
Canine chondrocytes seeded in type I and type II collagen implants investigated in vitro.
S. Nehrer (1997)
Scaffolds in tissue engineering bone and cartilage.
D. Hutmacher (2000)
The effect of gamma irradiation on injectable human amnion collagen.
B. Liu (1989)
Dynamic mechanical behavior of starch-based scaffolds in dry and physiologically simulated conditions: effect of porosity and pore size.
S. Ghosh (2008)
Feasibility study of a natural crosslinking reagent for biological tissue fixation.
H. Sung (1998)
In vivo biocompatibility and degradability of a novel injectable-chitosan-based implant.
F. Mi (2002)
A biomimetic three-dimensional woven composite scaffold for functional tissue engineering of cartilage.
F. T. Moutos (2007)
Biocompatibility study of a biological tissue fixed with a naturally occurring crosslinking reagent.
L. Huang (1998)

This paper is referenced by
In vitro evaluation of the biological performance of macro/micro-porous silk fibroin and silk-nano calcium phosphate scaffolds.
L-P Yan (2015)
Nanostructured hollow tubes based on chitosan and alginate multilayers.
Joana M. Silva (2014)
Evaluation of kappa carrageenan as potential carrier for floating drug delivery system: Effect of cross linker.
S. Selvakumaran (2015)
Investigating biomaterial-based biophysical cues for modulating macrophage polarization towards bone regeneration applications
Rukmani Sridharan (2018)
Biobased Composites for Medical and Industrial Applications
M. Fazley Elahi (2017)
Engineered tubular structures based on chitosan for tissue engineering applications
Joana M. Silva (2018)
Dual crosslinking strategy to generate mechanically viable cell-laden printable constructs using methacrylated collagen bioinks.
Nilabh S Kajave (2020)
Multifunctional biomaterials from the sea: Assessing the effects of chitosan incorporation into collagen scaffolds on mechanical and biological functionality.
R. Raftery (2016)
Silk fibroin-keratin based 3D scaffolds as a dermal substitute for skin tissue engineering.
N. Bhardwaj (2015)
Polyelectrolyte Multilayers for Applications in Hepatic Tissue Engineering
Margaret E. Cassin (2015)
Pre-clinical and clinical management of osteochondral lesions
Sandra Piña (2017)
In vitro characterization of injectable collagen and collagen-genipin hydrogels for neural tissue engineering
Jingwen Li (2016)
Fixation and Fixatives: Roles and Functions—A Short Review
Himanshu Singh (2019)
Isolation, characterization, and in vitro evaluation of bovine rumen submucosa films of collagen or chitosan-treated collagen
K Gopal Shankar (2016)
Fabrication, applications and challenges of natural biomaterials in tissue engineering
Saleem Ullah (2020)
Novel bioactive porous starch–siloxane matrix for bone regeneration: Physicochemical, mechanical, and in vitro properties
A. Aidun (2019)
Genipin crosslinking of cartilage enhances resistance to biochemical degradation and mechanical wear.
Megan E. McGann (2015)
Genipin-crosslinked electrospun chitosan nanofibers: Determination of crosslinking conditions and evaluation of cytocompatibility.
Q. Li (2015)
Flexible fibers wet-spun from formic acid modified chitosan.
J. Li (2016)
Is quercetin an alternative natural crosslinking agent to genipin for long-term dermal scaffolds implantation?
K. Greco (2018)
Chitosan based bioactive materials in tissue engineering applications-A review
Md. Sazedul Islam (2020)
The Short-Term Safety Evaluation of Corneal Crosslinking Agent-Genipin
Wenjing Song (2019)
Collagen and chitosan blends for 3D bioprinting: A rheological and printability approach
Ana Carolina Heidenreich (2020)
Porous chitosan scaffold cross-linked by chemical and natural procedure applied to investigate cell regeneration
Chih-Kai Yao (2012)
25th anniversary article: Designer hydrogels for cell cultures: a materials selection guide.
J. Thiele (2014)
Modified silk fibroin scaffolds with collagen/decellularized pulp for bone tissue engineering in cleft palate: Morphological structures and biofunctionalities.
Supaporn Sangkert (2016)
Challenges in Fabrication of Tissue-Engineered Cartilage with Correct Cellular Colonization and Extracellular Matrix Assembly
M. Lammi (2018)
lginate-and gelatin-based bioactive photocross-linkable hybrid aterials for bone tissue engineering
oanna Lewandowska-Łańcuckaa (2016)
Methods to create and characteristics of porous poly(vinyl) alcohol for the purpose of facial implants
Kathleen C. Bernhard (2014)
Comparison of two proanthocyanidin cross-linked recombinant human collagen-peptide (RHC) – chitosan scaffolds
Jing Zhang (2015)
Three-dimensional osteochondral microtissue to model pathogenesis of osteoarthritis
T. Lozito (2013)
Chitosan Extraction from Goliathus orientalis Moser, 1909: Characterization and Comparison with Commercially Available Chitosan
Pauline Fournier (2020)
See more
Semantic Scholar Logo Some data provided by SemanticScholar