Online citations, reference lists, and bibliographies.
← Back to Search

Novel Multicomponent Organic-inorganic WPI/gelatin/CaP Hydrogel Composites For Bone Tissue Engineering.

Michal Dziadek, R. Kudláčková, A. Zima, A. Ślósarczyk, M. Ziąbka, P. Jeleń, S. Shkarina, A. Cecilia, Marcus Zuber, T. Baumbach, M. Surmeneva, R. Surmenev, L. Bačáková, K. Cholewa-Kowalska, T. Douglas
Published 2019 · Materials Science, Medicine

Cite This
Download PDF
Analyze on Scholarcy
The present work focuses on the development of novel multicomponent organic-inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α-TCP in various concentrations (0-70 wt.%) was proposed. The results showed that α-TCP underwent incomplete transformation to calcium-deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30-70 wt.% α-TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α-TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14-day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast-like MG-63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications. This article is protected by copyright. All rights reserved.
This paper references
Preparation and Evaluation of Gelatin-Chitosan-Nanobioglass 3D Porous Scaffold for Bone Tissue Engineering
K. Maji (2016)
Hydrothermal synthesis of porous triphasic hydroxyapatite/(α and β) tricalcium phosphate
R. Vani (2009)
Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering.
H. Guo (2009)
Structural properties of starch-chitosan-gelatin foams and the impact of gelatin on MC3T3 mouse osteoblast cell viability
Gregory E. Risser (2017)
Characterization and evaluation of whey protein-based biofilms as substrates for in vitro cell cultures.
V. Gilbert (2005)
Comparison of cell behavior on pva/pva-gelatin electrospun nanofibers with random and aligned configuration
Chenyu Huang (2016)
Increasing Mechanical Strength of Gelatin Hydrogels by Divalent Metal Ion Removal
Q. Xing (2014)
Nano-hydroxyapatite/polyacrylamide composite hydrogels with high mechanical strengths and cell adhesion properties.
Z. Li (2014)
Physicochemical properties and biomimetic behaviour of α-TCP-chitosan based materials
J. Czechowska (2014)
Design of a multiphase osteochondral scaffold III: Fabrication of layered scaffolds with continuous interfaces.
B. Harley (2010)
FTIR and XRD evaluation of carbonated hydroxyapatite powders synthesized by wet methods
A. Ślósarczyk (2005)
The effect of synthetic α-tricalcium phosphate on osteogenic differentiation of rat bone mesenchymal stem cells.
Jinzhong Liu (2015)
Ca,P-rich layer formed on high-strength bioactive glass-ceramic A-W.
T. Kokubo (1990)
Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells.
Timothy E L Douglas (2018)
Functionalization of oligo(poly(ethylene glycol)fumarate) hydrogels with finely dispersed calcium phosphate nanocrystals for bone-substituting purposes
S. Leeuwenburgh (2007)
Tuning the structure of protein particles and gels with calcium or sodium ions.
Tuan Phan-Xuan (2013)
Calcium-Deficient Hydroxyapatite/Collagen/Platelet-Rich Plasma Scaffold with Controlled Release Function for Hard Tissue Regeneration
JiUn Lee (2018)
Preparation and characterization of gelatin hydrogel support for immobilization of Candida Rugosa lipase
M. Pulat (2013)
Raman Spectroscopy of Natural Bone and Synthetic Apatites
A. Khan (2013)
Highly extensible, tough, and elastomeric nanocomposite hydrogels from poly(ethylene glycol) and hydroxyapatite nanoparticles.
A. Gaharwar (2011)
Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods.
S. Koutsopoulos (2002)
Alpha-tricalcium phosphate synthesized by two different routes: Structural and spectroscopic characterization
J. Kolmas (2015)
Effect of whey protein on the proliferation and differentiation of osteoblasts.
R. Xu (2009)
Dual setting α-tricalcium phosphate cements
T. Christel (2012)
Alginate-based hydrogels with improved adhesive properties for cell encapsulation.
B. Sarker (2015)
Injectable thermosensitive hydrogel composite with surface-functionalized calcium phosphate as raw materials
Rangrang Fan (2014)
Whey protein as a key component in food systems: Physicochemical properties, production technologies and applications
R. Castro (2017)
β-Lactoglobulin and WPI aggregates: Formation, structure and applications
T. Nicolai (2011)
Sodium citrate as an effective dispersant for the synthesis of inorganic-organic composites with a nanodispersed mineral phase.
S. Leeuwenburgh (2010)
Aggregation Induced by Calcium Chloride and Subsequent Thermal Gelation of Whey Protein Isolate
Z. Ju (1998)
In vivo evaluation of whey protein-based biofilms as scaffolds for cutaneous cell cultures and biomedical applications.
M. Rouabhia (2007)
Effects of α-TCP and TetCP on MC3T3-E1 proliferation, differentiation and mineralization
A. Ehara (2003)
Rheological, mechanical and degradable properties of injectable chitosan/silk fibroin/hydroxyapatite/glycerophosphate hydrogels.
J. Wu (2016)
Hydrothermal synthesis of composites of well-crystallized hydroxyapatite and poly(vinyl alcohol) hydrogel
T. Goto (2012)
Use of whey proteins for encapsulation and controlled delivery applications
S. Gunasekaran (2007)
α-Tricalcium phosphate: synthesis, properties and biomedical applications.
R. G. Carrodeguas (2011)
Inhibition and Promotion of Heat-Induced Gelation of Whey Proteins in the Presence of Calcium by Addition of Sodium Caseinate.
Bach T. Nguyen (2016)
Microwave accelerated synthesis of nanosized calcium deficient hydroxyapatite
A. Siddharthan (2004)
Preparation of hydroxyapatite-gelatin nanocomposite.
M. C. Chang (2003)
The Effect of Addition of Calcium Phosphate Particles to Hydrogel‐Based Composite Materials on Stiffness and Differentiation of Mesenchymal Stromal Cells toward Osteogenesis
Kshama S Sen (2018)
Hybrid multicomponent hydrogels for tissue engineering.
X. Jia (2009)
Processing of whey from dairy industry waste
S. Ostojić (2005)
Synthesis, characterization, and dispersion properties of hydroxyapatite prepared by mechanochemical-hydrothermal methods
C. Chen (2004)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar