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Novel Multicomponent Organic-inorganic WPI/gelatin/CaP Hydrogel Composites For Bone Tissue Engineering.

Michal Dziadek, R. Kudláčková, A. Zima, A. Ślósarczyk, M. Ziąbka, P. Jeleń, S. Shkarina, A. Cecilia, Marcus Zuber, T. Baumbach, M. Surmeneva, R. Surmenev, L. Bačáková, K. Cholewa-Kowalska, T. Douglas
Published 2019 · Materials Science, Medicine

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The present work focuses on the development of novel multicomponent organic-inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α-TCP in various concentrations (0-70 wt.%) was proposed. The results showed that α-TCP underwent incomplete transformation to calcium-deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30-70 wt.% α-TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α-TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14-day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast-like MG-63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications. This article is protected by copyright. All rights reserved.
This paper references
10.1155/2016/9825659
Preparation and Evaluation of Gelatin-Chitosan-Nanobioglass 3D Porous Scaffold for Bone Tissue Engineering
K. Maji (2016)
10.1007/s10856-008-3480-8
Hydrothermal synthesis of porous triphasic hydroxyapatite/(α and β) tricalcium phosphate
R. Vani (2009)
10.1016/j.actbio.2008.07.018
Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering.
H. Guo (2009)
10.1186/s13036-017-0086-z
Structural properties of starch-chitosan-gelatin foams and the impact of gelatin on MC3T3 mouse osteoblast cell viability
Gregory E. Risser (2017)
10.1016/J.BIOMATERIALS.2005.05.085
Characterization and evaluation of whey protein-based biofilms as substrates for in vitro cell cultures.
V. Gilbert (2005)
10.1038/srep37960
Comparison of cell behavior on pva/pva-gelatin electrospun nanofibers with random and aligned configuration
Chenyu Huang (2016)
10.1038/srep04706
Increasing Mechanical Strength of Gelatin Hydrogels by Divalent Metal Ion Removal
Q. Xing (2014)
10.1016/j.colsurfb.2014.10.050
Nano-hydroxyapatite/polyacrylamide composite hydrogels with high mechanical strengths and cell adhesion properties.
Z. Li (2014)
10.1016/J.CERAMINT.2013.10.142
Physicochemical properties and biomimetic behaviour of α-TCP-chitosan based materials
J. Czechowska (2014)
10.1002/jbm.a.32387
Design of a multiphase osteochondral scaffold III: Fabrication of layered scaffolds with continuous interfaces.
B. Harley (2010)
10.1016/J.MOLSTRUC.2004.11.078
FTIR and XRD evaluation of carbonated hydroxyapatite powders synthesized by wet methods
A. Ślósarczyk (2005)
The effect of synthetic α-tricalcium phosphate on osteogenic differentiation of rat bone mesenchymal stem cells.
Jinzhong Liu (2015)
10.1002/JBM.820240306
Ca,P-rich layer formed on high-strength bioactive glass-ceramic A-W.
T. Kokubo (1990)
10.3168/jds.2017-13119
Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells.
Timothy E L Douglas (2018)
10.1163/156856207794761998
Functionalization of oligo(poly(ethylene glycol)fumarate) hydrogels with finely dispersed calcium phosphate nanocrystals for bone-substituting purposes
S. Leeuwenburgh (2007)
10.1021/bm400347d
Tuning the structure of protein particles and gels with calcium or sodium ions.
Tuan Phan-Xuan (2013)
10.1021/ACSBIOMATERIALS.7B00640
Calcium-Deficient Hydroxyapatite/Collagen/Platelet-Rich Plasma Scaffold with Controlled Release Function for Hard Tissue Regeneration
JiUn Lee (2018)
10.3109/10731199.2012.696070
Preparation and characterization of gelatin hydrogel support for immobilization of Candida Rugosa lipase
M. Pulat (2013)
10.1080/05704928.2012.721107
Raman Spectroscopy of Natural Bone and Synthetic Apatites
A. Khan (2013)
10.1021/bm200027z
Highly extensible, tough, and elastomeric nanocomposite hydrogels from poly(ethylene glycol) and hydroxyapatite nanoparticles.
A. Gaharwar (2011)
10.1002/JBM.10280
Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods.
S. Koutsopoulos (2002)
10.1016/J.CERAMINT.2014.12.159
Alpha-tricalcium phosphate synthesized by two different routes: Structural and spectroscopic characterization
J. Kolmas (2015)
10.3168/jds.2008-1702
Effect of whey protein on the proliferation and differentiation of osteoblasts.
R. Xu (2009)
10.1007/s10856-012-4828-7
Dual setting α-tricalcium phosphate cements
T. Christel (2012)
10.1016/j.ijbiomac.2015.03.061
Alginate-based hydrogels with improved adhesive properties for cell encapsulation.
B. Sarker (2015)
10.2147/IJN.S52689
Injectable thermosensitive hydrogel composite with surface-functionalized calcium phosphate as raw materials
Rangrang Fan (2014)
10.1016/J.FOOSTR.2017.05.004
Whey protein as a key component in food systems: Physicochemical properties, production technologies and applications
R. Castro (2017)
10.1016/J.FOODHYD.2011.02.006
β-Lactoglobulin and WPI aggregates: Formation, structure and applications
T. Nicolai (2011)
10.1016/j.actbio.2009.09.005
Sodium citrate as an effective dispersant for the synthesis of inorganic-organic composites with a nanodispersed mineral phase.
S. Leeuwenburgh (2010)
10.3168/JDS.S0022-0302(98)75652-8
Aggregation Induced by Calcium Chloride and Subsequent Thermal Gelation of Whey Protein Isolate
Z. Ju (1998)
10.1088/1748-6041/2/1/S06
In vivo evaluation of whey protein-based biofilms as scaffolds for cutaneous cell cultures and biomedical applications.
M. Rouabhia (2007)
10.1016/S0142-9612(02)00411-8
Effects of α-TCP and TetCP on MC3T3-E1 proliferation, differentiation and mineralization
A. Ehara (2003)
10.1016/j.jmbbm.2016.07.007
Rheological, mechanical and degradable properties of injectable chitosan/silk fibroin/hydroxyapatite/glycerophosphate hydrogels.
J. Wu (2016)
10.1016/J.MSEC.2011.11.011
Hydrothermal synthesis of composites of well-crystallized hydroxyapatite and poly(vinyl alcohol) hydrogel
T. Goto (2012)
10.1016/J.JFOODENG.2006.11.001
Use of whey proteins for encapsulation and controlled delivery applications
S. Gunasekaran (2007)
10.1016/j.actbio.2011.06.019
α-Tricalcium phosphate: synthesis, properties and biomedical applications.
R. G. Carrodeguas (2011)
10.1021/ACS.BIOMAC.6B01322
Inhibition and Promotion of Heat-Induced Gelation of Whey Proteins in the Presence of Calcium by Addition of Sodium Caseinate.
Bach T. Nguyen (2016)
10.1007/S10856-004-5735-3
Microwave accelerated synthesis of nanosized calcium deficient hydroxyapatite
A. Siddharthan (2004)
10.1016/S0142-9612(03)00115-7
Preparation of hydroxyapatite-gelatin nanocomposite.
M. C. Chang (2003)
10.1002/adhm.201800343
The Effect of Addition of Calcium Phosphate Particles to Hydrogel‐Based Composite Materials on Stiffness and Differentiation of Mesenchymal Stromal Cells toward Osteogenesis
Kshama S Sen (2018)
10.1002/mabi.200800284
Hybrid multicomponent hydrogels for tissue engineering.
X. Jia (2009)
10.1007/S10311-005-0108-9
Processing of whey from dairy industry waste
S. Ostojić (2005)
10.1039/B315095J
Synthesis, characterization, and dispersion properties of hydroxyapatite prepared by mechanochemical-hydrothermal methods
C. Chen (2004)



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