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Recent Advances In CRISPR‐Cas9 Genome Editing Technology For Biological And Biomedical Investigations

V. Singh, Nisarg Gohil, Robert Ramírez García, D. Braddick, Christian Kuete Fofié
Published 2018 · Biology, Medicine

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The Type II CRISPR‐Cas9 system is a simple, efficient, and versatile tool for targeted genome editing in a wide range of organisms and cell types. It continues to gain more scientific interest and has established itself as an extremely powerful technology within our synthetic biology toolkit. It works upon a targeted site and generates a double strand breaks that become repaired by either the NHEJ or the HDR pathway, modifying or permanently replacing the genomic target sequences of interest. These can include viral targets, single‐mutation genetic diseases, and multiple‐site corrections for wide scale disease states, offering the potential to manage and cure some of mankind's most persistent biomedical menaces. Here, we present the developing progress and future potential of CRISPR‐Cas9 in biological and biomedical investigations, toward numerous therapeutic, biomedical, and biotechnological applications, as well as some of the challenges within. J. Cell. Biochem. 119: 81–94, 2018. © 2017 Wiley Periodicals, Inc.
This paper references
10.1210/me.2014-1288
Editorial: advances in neuroscience: the BRAIN initiative and implications for neuroendocrinology.
U. Kaiser (2014)
10.3389/fncir.2014.00149
Visual cortical areas of the mouse: comparison of parcellation and network structure with primates
M. Laramée (2015)
10.1099/vir.0.000139
Inhibition of HIV-1 infection of primary CD4+ T-cells by gene editing of CCR5 using adenovirus-delivered CRISPR/Cas9.
C. Li (2015)
10.7150/ijbs.17421
CRISPR-Cas9: from Genome Editing to Cancer Research
S. Chen (2016)
10.1126/science.1225829
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
M. Jinek (2012)
10.1073/pnas.91.10.4509
Krüppel-associated boxes are potent transcriptional repression domains.
J. F. Margolin (1994)
10.1126/science.1231143
Multiplex Genome Engineering Using CRISPR/Cas Systems
L. Công (2013)
10.1681/ASN.2004090754
Kidney development and disease in the zebrafish.
I. Drummond (2005)
10.1038/srep22555
Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing
Rafal Kamiński (2016)
10.1021/acssynbio.5b00264
Multiple Gene Repression in Cyanobacteria Using CRISPRi.
L. Yao (2016)
10.1128/mBio.00928-13
Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems
Ahmed A. Gomaa (2014)
10.1126/science.1178817
A Simple Cipher Governs DNA Recognition by TAL Effectors
M. Moscou (2009)
10.1038/nbt.3439
A CRISPR-Cas9 Gene Drive System Targeting Female Reproduction in the Malaria Mosquito vector Anopheles gambiae
Andrew M. Hammond (2016)
10.1038/mtna.2014.38
The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo
Su-Ru Lin (2014)
10.1073/pnas.1500107112
Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria
Ido Yosef (2015)
10.1016/j.celrep.2014.03.017
Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
Andrew R. Bassett (2014)
10.1016/j.celrep.2014.09.044
Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila.
X. Ren (2014)
10.1128/JB.01412-07
Phage response to CRISPR-encoded resistance in Streptococcus thermophilus.
H. Deveau (2008)
10.1016/B978-0-12-394621-8.00011-X
Role of CRISPR/cas system in the development of bacteriophage resistance.
A. Szczepankowska (2012)
10.1038/nchembio.1753
A light-inducible CRISPR/Cas9 system for control of endogenous gene activation
Lauren R. Polstein (2015)
10.1038/nrc3950
Applications of the CRISPR–Cas9 system in cancer biology
Francisco J. Sánchez-Rivera (2015)
10.1016/j.cell.2013.09.040
Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
F. Ran (2013)
10.1111/j.1365-2958.1995.mmi_17010085.x
Long stretches of short tandem repeats are present in the largest replicons of the Archaea Haloferax mediterranei and Haloferax volcanii and could be involved in replicon partitioning.
F. Mojica (1995)
10.1016/S0140-6736(10)61029-X
Sickle-cell disease
D. Rees (2010)
CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies.
C. Pourcel (2005)
10.1093/NAR/30.2.482
A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysis.
K. Makarova (2002)
10.1002/0471142727.mb3102s111
CRISPR‐Cas9 Genome Editing in Drosophila
Scott J Gratz (2015)
10.1038/nm.3793
Therapeutic genome editing: prospects and challenges
D. Cox (2015)
10.1038/nmeth.2600
RNA-guided gene activation by CRISPR-Cas9-based transcription factors
P. Perez-Pinera (2013)
10.1073/pnas.1521077112
Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito Anopheles stephensi
Valentino M. Gantz (2015)
10.1534/g3.116.036723
Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy
Nanci S Kane (2016)
10.1016/S0092-8674(00)81683-9
The Hallmarks of Cancer
D. Hanahan (2000)
10.1101/gr.145441.112
Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining.
Marcello Maresca (2013)
10.1517/14712598.2015.1036736
The therapeutic application of CRISPR/Cas9 technologies for HIV
Sheena M Saayman (2015)
10.1126/science.1178811
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
J. Boch (2009)
10.1038/srep20620
One-step generation of triple gene-targeted pigs using CRISPR/Cas9 system
X. Wang (2016)
10.4161/nucl.28488
Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system
Tobias Anton (2014)
10.1038/nbt1317
Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases
M. Szczepek (2007)
10.1016/j.gene.2016.11.008
Exploring the potential of genome editing CRISPR-Cas9 technology.
V. Singh (2017)
10.1038/mt.2016.24
CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape
G. Wang (2016)
10.1016/S0084-3954(08)79143-7
Prophylaxis versus Episodic Treatment to Prevent Joint Disease in Boys with Severe Hemophilia
J. A. Stockman (2009)
10.1016/j.celrep.2015.07.061
Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate.
P. Srikanth (2015)
10.1038/nbt.2508
RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
Wenyan Jiang (2013)
10.1038/nmeth.2598
CRISPR RNA-guided activation of endogenous human genes
Morgan L. Maeder (2013)
10.1038/ncomms7244
Multiplex CRISPR/Cas9-Based Genome Editing for Correction of Dystrophin Mutations that Cause Duchenne Muscular Dystrophy
David G. Ousterout (2015)
10.1101/020891
Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing
Hiroki Ando (2015)
10.4414/smw.2015.14230
The CRISPR revolution and its impact on cancer research.
R. Kannan (2015)
and M Caudy protein-protein interaction domain . 4-amino-acid transcription repression and helix-loop-helix repressor proteins acts as a The WRPW motif of the hairy-related
A. Fisher (1996)
10.1093/BRAIN/122.4.593
The neuropathology of schizophrenia. A critical review of the data and their interpretation.
Paul J. Harrison (1999)
10.1016/j.cell.2013.12.001
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
Baohui Chen (2013)
10.1038/nature09886
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
Elitza Deltcheva (2011)
Activated CD4CCR5 T cells
NY Sardesai (2015)
10.1038/ncomms7267
Gene silencing by CRISPR interference in mycobacteria.
Eira Choudhary (2015)
10.1038/nbt.1767
TALEs of genome targeting
J. Boch (2011)
10.1126/science.1247005
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
O. Shalem (2014)
10.1371/journal.pone.0002652
Insight into Microevolution of Yersinia pestis by Clustered Regularly Interspaced Short Palindromic Repeats
Y. Cui (2008)
10.1186/1866-1955-4-21
Of mice and monkeys: using non-human primate models to bridge mouse- and human-based investigations of autism spectrum disorders
K. K. Watson (2012)
10.1016/j.jbiotec.2015.04.024
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection.
X. Liang (2015)
10.1126/science.1159689
Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes
Stan J J Brouns (2008)
10.1074/jbc.M113.539726
Cut Site Selection by the Two Nuclease Domains of the Cas9 RNA-guided Endonuclease*
Hongfan Chen (2014)
10.1038/nature21405
Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection
J. Eyquem (2017)
10.1016/j.antiviral.2015.03.015
Targeting hepatitis B virus cccDNA by CRISPR/Cas9 nuclease efficiently inhibits viral replication.
C. Dong (2015)
10.1534/g3.114.014126
Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila
Joseph Gokcezade (2014)
10.1046/J.1365-2958.2002.02839.X
Identification of genes that are associated with DNA repeats in prokaryotes.
R. Jansen (2002)
10.1016/j.celrep.2016.03.042
CRISPR/Cas9-Derived Mutations Both Inhibit HIV-1 Replication and Accelerate Viral Escape.
Zhen Wang (2016)
10.1093/nar/gkr606
The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
Rimantas Šapranauskas (2011)
10.1073/PNAS.98.2.525
Genetic architecture of thermal adaptation in Escherichia coli.
M. Riehle (2001)
10.1186/s13059-014-0516-x
A CRISPR design for next-generation antimicrobials
Chase L. Beisel (2014)
TAL-type III effectors. Science 326(5959):1509–12
A Bolotin (2011)
10.1099/mic.0.28048-0
Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin.
A. Bolotin (2005)
10.1016/j.cell.2013.04.025
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
H. Wang (2013)
10.1016/j.cell.2013.06.044
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
L. Gilbert (2013)
10.1038/nbt.2501
Efficient genome editing in zebrafish using a CRISPR-Cas system.
Woong Y. Hwang (2013)
10.2174/1570162X13666150807105718
Perspectives of Genome-Editing Technologies for HIV Therapy
Hirotaka Ebina (2016)
10.1128/JB.188.7.2364-2374.2006
Chromosome evolution in the Thermotogales: large-scale inversions and strain diversification of CRISPR sequences.
R. Deboy (2006)
10.1177/1742271X9300100414
Dynamic Imaging
(1993)
10.1038/nature20565
In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration
Keiichiro Suzuki (2016)
10.7554/eLife.00471
RNA-programmed genome editing in human cells
M. Jinek (2013)
10.1038/srep02510
Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus
Hirotaka Ebina (2013)
10.1158/2159-8290.CD-12-0548
The basic principles of chimeric antigen receptor design.
M. Sadelain (2013)
10.1016/j.cell.2013.02.022
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Lei S. Qi (2013)
10.1016/S0140-6736(07)61416-0
Human papillomavirus and cervical cancer
M. Schiffman (2007)
Applications of CRISPR-Cas systems in neuroscience
C. Harvard (2016)
10.1046/J.1365-2958.2000.01838.X
Biological significance of a family of regularly spaced repeats in the genomes of Archaea, Bacteria and mitochondria.
F. Mojica (2000)
10.1038/nn.2514
Dnmt1 and Dnmt3a maintain DNA methylation and regulate synaptic function in adult forebrain neurons
Jian Feng (2010)
Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA
F. Gleason (2014)
10.1523/JNEUROSCI.0758-12.2012
Fgf-Dependent Glial Cell Bridges Facilitate Spinal Cord Regeneration in Zebrafish
Y. Goldshmit (2012)
10.1073/pnas.1420024112
Multicolor CRISPR labeling of chromosomal loci in human cells
H. Ma (2015)
10.3389/fcimb.2017.00091
Removal of Integrated Hepatitis B Virus DNA Using CRISPR-Cas9
Hao Li (2017)
10.1093/alcalc/agv110
Long-Term Alcohol Consumption and Breast, Upper Aero-Digestive Tract and Colorectal Cancer Risk: A Systematic Review and Meta-Analysis.
H. Jayasekara (2016)
10.1186/s13024-015-0031-x
CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases
Zhuchi Tu (2015)
10.1126/science.aad5143
In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy
C. E. Nelson (2016)
10.1073/pnas.1422340112
Cas9-mediated targeting of viral RNA in eukaryotic cells
Aryn A. Price (2015)
10.1101/gr.173427.114
Seamless gene correction of β-thalassemia mutations in patient-specific iPSCs using CRISPR/Cas9 and piggyBac.
F. Xie (2014)
10.1038/srep08841
Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
Y. Hisano (2015)
10.1126/science.1247997
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
M. Jinek (2014)
10.1146/annurev-genet-110410-132430
CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation.
D. Bhaya (2011)
10.1126/science.1165771
CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA
Luciano A Marraffini (2008)
10.1146/annurev.micro.112408.134123
CRISPR/Cas system and its role in phage-bacteria interactions.
H. Deveau (2010)
10.1016/j.cell.2014.09.014
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Randall J Platt (2014)
10.1097/00005072-199911000-00009
The neuropathology of schizophrenia.
K. Jellinger (1999)
10.1016/j.cell.2012.03.052
Dynamics and Memory of Heterochromatin in Living Cells
Nathaniel A. Hathaway (2012)
10.1073/pnas.1700622114
CRISPR-Cas9–guided oncogenic chromosomal translocations with conditional fusion protein expression in human mesenchymal cells
Fabio Vanoli (2017)
10.1038/nbt.3081
Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo
J. Zuris (2015)
10.1146/ANNUREV.BIOCHEM.70.1.313
Design and selection of novel Cys2His2 zinc finger proteins.
C. Pabo (2001)
10.1038/srep44929
Genetic engineering of a temperate phage-based delivery system for CRISPR/Cas9 antimicrobials against Staphylococcus aureus
J. Y. Park (2017)
RNA-guided endonuclease
S Chen (2016)
10.1093/jnci/djw320
Durable Control of Metastatic AKT1-Mutant WHO Grade 1 Meningothelial Meningioma by the AKT Inhibitor, AZD5363.
M. Weller (2017)
10.1126/science.1232033
RNA-Guided Human Genome Engineering via Cas9
P. Mali (2013)
10.1093/nar/gkt520
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
David Bikard (2013)
10.1128/JVI.01879-14
Inactivation of the Human Papillomavirus E6 or E7 Gene in Cervical Carcinoma Cells by Using a Bacterial CRISPR/Cas RNA-Guided Endonuclease
E. M. Kennedy (2014)
10.1371/journal.pone.0115987
CCR5 Gene Disruption via Lentiviral Vectors Expressing Cas9 and Single Guided RNA Renders Cells Resistant to HIV-1 Infection
W. Wang (2014)
10.3748/wjg.v20.i34.12056
Individualized management of pregnant women with high hepatitis B virus DNA levels.
Z. Zhang (2014)
10.1101/GR.169101
A systematic analysis of human disease-associated gene sequences in Drosophila melanogaster.
L. Reiter (2001)
10.1038/tp.2015.203
CRISPR/Cas9 facilitates investigation of neural circuit disease using human iPSCs: mechanism of epilepsy caused by an SCN1A loss-of-function mutation
J. Liu (2016)
RNA-guided nucleases
L Cong (2013)
10.3390/v4102291
Function and Regulation of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) / CRISPR Associated (Cas) Systems
Corinna Richter (2012)
10.1534/genetics.111.131433
Genome Engineering With Zinc-Finger Nucleases
D. Carroll (2011)
10.1016/j.virol.2014.12.001
Suppression of hepatitis B virus DNA accumulation in chronically infected cells using a bacterial CRISPR/Cas RNA-guided DNA endonuclease.
E. M. Kennedy (2015)
10.15252/emmm.201506039
CRISPR/Cas9‐mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse
Yuting Guan (2016)
10.1002/jcb.26099
CRISPR Editing Technology in Biological and Biomedical Investigation
M. White (2017)
10.1038/nbt.3055
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9
Lukasz Swiech (2015)
10.1038/nbt.3469
A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice
Yang Yang (2016)
10.1186/s12977-016-0270-0
CRISPR/Cas9: a double-edged sword when used to combat HIV infection
Chen Liang (2016)
10.1038/nbt.2884
Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype
H. Yin (2014)
10.1007/978-3-319-34148-4
Genome Editing
K. Turksen (2016)
10.1016/j.ymben.2015.01.008
Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae.
T. Jakočiu̅nas (2015)
10.1534/genetics.113.156737
Highly Improved Gene Targeting by Germline-Specific Cas9 Expression in Drosophila
S. Kondo (2013)
10.1186/s12977-015-0150-z
The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA
Weijun Zhu (2015)
10.1038/nbt.3011
Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
Robert J Citorik (2014)
10.1038/nbt.3043
Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials
David Bikard (2014)
10.15252/msb.20145735
Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks
Alec A. K. Nielsen (2014)
10.1038/nature09523
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
Josiane E. Garneau (2010)
10.1093/nar/gkt135
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
James E. DiCarlo (2013)
10.1038/nature09806
The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset
C. Ceol (2011)
10.1093/jnci/djw183
Inactivation of Cancer Mutations Utilizing CRISPR/Cas9.
Christina Gebler (2017)
10.1038/gt.2015.2
Harnessing the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system to disrupt the hepatitis B virus
S. Zhen (2015)
10.1016/j.cell.2009.07.040
RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex
Caryn R. Hale (2009)
10.1128/jb.169.12.5429-5433.1987
Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product.
Y. Ishino (1987)
10.1111/gtc.12154
Multiple genome modifications by the CRISPR/Cas9 system in zebrafish
S. Ota (2014)
10.1126/science.1138140
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
R. Barrangou (2007)
10.1186/1745-6150-1-29
The ancient Virus World and evolution of cells
E. Koonin (2006)
Breaking the code of DNA
J Boch (2009)
10.1146/annurev.biochem.78.082907.145923
Multidrug resistance in bacteria.
H. Nikaido (2009)
10.1158/2326-6066.CIR-15-0064
The Interplay of Immunotherapy and Chemotherapy: Harnessing Potential Synergies
L. Emens (2015)
10.1016/S0140-6736(12)61689-4
Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990–2010: a systematic analysis for the Global Burden of Disease Study 2010
Christopher J. L. Murray (2013)
10.4103/1008-682X.157399
Reprogrammed CRISPR-Cas9 targeting the conserved regions of HPV6/11 E7 genes inhibits proliferation and induces apoptosis in E7-transformed keratinocytes
Y. Liu (2016)
10.1186/s13578-015-0027-9
Application of CRISPR/Cas9 for biomedical discoveries
S. Riordan (2015)
10.1126/science.1179555
CRISPR/Cas, the Immune System of Bacteria and Archaea
P. Horvath (2010)
10.1016/j.ymthe.2017.03.012
In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models.
Chaoran Yin (2017)
10.1161/ATVBAHA.116.304790
A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research.
J. Miano (2016)
10.1038/nbt.2661
Heritable gene targeting in the mouse and rat using a CRISPR-Cas system.
Dali Li (2013)
10.15252/embr.201642378
Linear ubiquitination by LUBEL has a role in Drosophila heat stress response
Tomoko Asaoka (2016)
10.1038/nature13589
CRISPR-mediated direct mutation of cancer genes in the mouse liver
W. Xue (2014)
10.1073/pnas.1407466112
Activated CD4+CCR5+ T cells in the rectum predict increased SIV acquisition in SIVGag/Tat-vaccinated rhesus macaques
D. Carnathan (2014)
10.1126/science.1258096
The new frontier of genome engineering with CRISPR-Cas9
J. Doudna (2014)
10.1515/bc.2011.042
Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism in prokaryotes
Sinan Al-Attar (2011)
10.1186/s13229-015-0048-6
CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment
P. Wang (2015)
10.1073/pnas.1308335110
Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system
Li-En Jao (2013)
10.3748/wjg.v20.i24.7696
Human genes involved in hepatitis B virus infection.
Z. Zeng (2014)
10.1016/j.cell.2014.05.010
Development and Applications of CRISPR-Cas9 for Genome Engineering
Patrick D. Hsu (2014)
10.1007/s00239-004-0046-3
Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements
F. Mojica (2004)
10.1016/j.cell.2014.02.001
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
H. Nishimasu (2014)
10.1038/emboj.2011.41
Cas3 is a single‐stranded DNA nuclease and ATP‐dependent helicase in the CRISPR/Cas immune system
Tomas Šinkūnas (2011)
10.1038/ncomms7413
Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells.
Hsin-Kai Liao (2015)
10.1212/WNL.0000000000000610
The global burden of neurologic diseases
J. Chin (2014)
10.1016/j.coi.2015.01.002
Designing chimeric antigen receptors to effectively and safely target tumors.
M. Jensen (2015)
2013.One-step generation of mice carrying mutations in multiple genes by CRISPR-Cas-mediated genome engineering. Cell 153:910–8
H Wang (2013)



This paper is referenced by
10.1007/978-981-15-0081-7
Advances in Synthetic Biology
Linda J. Kahl (2016)
10.3389/fvets.2017.00237
Antimicrobial Resistance: Its Surveillance, Impact, and Alternative Management Strategies in Dairy Animals
C. Sharma (2018)
10.3389/fmicb.2019.02471
Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials: Review and Prospects
Yingjun Li (2019)
10.3389/fbioe.2019.00248
Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems
Khushal Khambhati (2019)
10.1002/9781119509967.CH12
Genome Editing: New Breeding Technologies in Plants
Kalyani M. Barbadikar (2019)
10.1002/jcb.26198
Progress and Application of CRISPR/Cas Technology in Biological and Biomedical Investigation
Jiachen Lin (2017)
10.1007/978-981-15-0081-7_1
Introduction to Synthetic Biology
Vijai Singh (2020)
10.1002/jcb.26361
New insight into the control of peptic ulcer by targeting the histamine H2 receptor
V. Singh (2018)
10.3389/fbioe.2019.00050
Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities
Nisarg Gohil (2019)
10.1007/5584_2019_473
New Pioneers of Optogenetics in Neuroscience.
Ö. S. Somuncu (2020)
10.1371/journal.pone.0195558
Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells
Thomas Del'Guidice (2018)
10.3389/fmicb.2019.00087
Gene Editing and Systems Biology Tools for Pesticide Bioremediation: A Review
Shweta Jaiswal (2019)
10.1002/jcb.27690
Current progress in CRISPR‐based diagnostic platforms
Khushal Khambhati (2019)
10.3389/fcimb.2018.00106
Book Review: Quorum Sensing vs. Quorum Quenching: A Battle With No End in Sight
Nisarg Gohil (2018)
10.3389/fbioe.2017.00071
Book Review: Recent Advances in Yeast Metabolic Engineering
Nisarg Gohil (2017)
10.1007/978-981-15-0081-7_13
Recent Advances, Challenges, and Opportunities in Synthetic Genomes
Vijai Singh (2020)
10.1002/ar.24107
Recent Advancements in Gene and Stem Cell‐Based Treatment Modalities: Potential Implications in Noise‐Induced Hearing Loss
A. Eshraghi (2019)
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