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Phagocytosis And Killing Of Cryptococcus Neoformans By Rat Alveolar Macrophages In The Absence Of Serum

B. Bolaños, T. Mitchell
Published 1989 · Biology, Medicine

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The in vitro interaction between yeast cells of Cryptococcus neoformans and Lewis rat alveolar macrophages (AMÕ) was studied in the absence of serum. AMÕ were harvested by lung lavage, and monolayers of adherent cells were established in wells of microtiter plates. Radiolabeled yeast cells were added to fresh AMÕ monolayers, the plates were incubated at 37°C under 5% CO2, nonadherent yeasts were removed, and phagocytosis (i.e., attachment or ingestion) was determined by measuring adherent radioactivity. AMÕ were able to bind or ingest, and kill, encapsulated strains of C. neoformans in the absence of serum. Serum‐free phagocytosis was suboptimal by comparison with phagocytosis in the presence of serum. The mechanism of serum‐free phagocytosis Involves a receptor on the AMÕ with affinity for mannose‐rich determinants present on the yeast cell walls and unrelated to the capsular polysaccharide. Opsonin‐independent phagocytosis was only detected with nonencapsulated, small, and medium encapsulated strains of C. neoformans. Large encapsulated strains were not taken up without serum. Serum‐free phagocytosis could be of critical importance in the alveolar spaces, where only marginal concentrations of serum opsonins are initially present.
This paper references
In vitro phagocytosis and intracellular fate of variously encapsulated strains of Cryptococcus neoformans.
T. Mitchell (1972)
Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium.
D. Mosier (1981)
Killing of Cryptococcus neoformans by rat alveolar macrophages.
B. Bolaños (1989)
Analysis of proteins and respiratory cells obtained from human lungs by lung lavage
H. Y. Reynolds (1974)
Untersuchungen zur elektronenmikroskopischen Darstellung der Kapsel von Cryptococcus neoformans. Ultrastructural Research on the Capsule of Cryptococcus neoformans
R. Weiß (1987)
A macrophage receptor for (mannose/glucosamine)-glycoproteins of potential importance in phagocytic activity.
G. Warr (1980)
Yeast mannans inhibited binding and phagocytosis of zymosan by mouse peritoneal macrophages
S-S. J. Sung (1983)
Airborne Cryptococcus neoformans: particles from pigeon excreta compatible with alveolar deposition.
K. Powell (1972)
Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage.
H. Reynolds (1974)
The evolution of pulmonary cryptococcosis: clinical implications from a study of 41 patients with and without compromising host factors.
T. Kerkering (1981)
Strain variation in composition and molecular size of the capsular polysaccharide of Cryptococcus neoformans serotype A.
J. Small (1986)
Chloroquine and ammonium ion inhibit receptor-mediated endocytosis of mannose-glycoconjugates by macrophages: apparent inhibition of receptor recycling.
C. Tietze (1980)
Yeast Mannans inhibit binding and phagocytosis of zymosan by mouse peritoneal macrophages
SJ Sung (1983)
Binding of cryptococcal polysaccharide to Cryptococcus neoformans.
T. Kozel (1984)
Tissue changes and tissue diagnosis in cryptococcosis . A study of twenty - six cases
R. K. Haugen (1955)
Nonencapsulated Variant of Cryptococcus neoformans I. Virulence Studies and Characterization of Soluble Polysaccharide.
T. Kozel (1971)
Opsonin‐Independent Phagocytosis of Surface‐Adherent Bacteria by Human Alveolar Macrophages
D. A. Lee (1984)
Pathogenesis of cardiac rupture due to myocardial infarction; a study of twenty-six cases.
J. Lunseth (1956)
Inflammatory and immune processes of the human lung in health and disease: evaluation by pulmonary layage
B. W. Hunninghale (1979)
Untersuchungen zur elek - tronenmikroskopischen Darstellung der Kapsel von Crvptococcus neoformans
R. Weiss
An evaluation of various environmental factors affecting the propagation of Crvpneoformans
Ishaq (1967)
Phagocytosis of Cryptococcus neoformans by rat alveolar macrophages.
B. Bolaños (1989)
Endocytosis and the recycling of plasma membrane
R. Steinman (1983)
Binding of purified and radioiodinated capsular polysaccharides from Cryptococcus neoformans serotype A strains to capsule-free mutants.
J. Small (1986)
Tissue changes and tissue diagnosis in cryptococcosis; a study of 26 cases.
R. D. Baker (1955)
Micromorphology of Crvptococcus neoformans
M. R. Edwards (1967)
Inflammatory and immune processes of the human lung in health and disease : evaluation by pulmonary lay
B. W. Hunninghale (1979)

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Quantitative analysis of apoptotic cell death in granulomatous inflammation induced by intravenous challenge with Cryptococcus neoformans and bacillus Calmette‐Guérin vaccine
K. Matsunaga (2000)
The interaction of Cryptococcus neoformans with primary rat lung cell cultures.
G. Merkel (1992)
Interferon-γ Activates Rat Alveolar Macrophages for Anticryptococcal Activity
C. Mody (1991)
in Vitro Effect of Lung Surfactant on Alveolar Macrophage Defence Mechanisms Against Cryptococcus Neoformans
N. Gross (2004)
Are Macrophages the Heroes or Villains During Cryptococcosis?
Jacob Rudman (2019)
Primary Dendritic Cells Phagocytose Cryptococcus neoformans via Mannose Receptors and Fcγ Receptor II for Presentation to T Lymphocytes
R. Syme (2002)
Induction of Capsule Growth in Cryptococcus neoformans by Mammalian Serum and CO 2
U. Banerjee (2003)
Reduced binding and phagocytosis of Pneumocystis carinii by alveolar macrophages from persons infected with HIV-1 correlates with mannose receptor downregulation.
H. Koziel (1998)
Blood-brain barrier invasion by Cryptococcus neoformans is enhanced by functional interactions with plasmin.
Jamal Stie (2012)
Mechanisms of brain infection by the human fungalpathogen Cryptococcus neoformans
Wilber Sabiiti (2012)
In Vivo Role of Dendritic Cells in a Murine Model of Pulmonary Cryptococcosis
K. Wozniak (2006)
An Innate Immune System Cell Is a Major Determinant of Species-Related Susceptibility Differences to Fungal Pneumonia1
Xiuping Shao (2005)
Aggregates of ultrafine particles impair phagocytosis of microorganisms by human alveolar macrophages.
M. Lundborg (2006)
Mechanisms of Pulmonary Escape and Dissemination by Cryptococcus neoformans
Steven T. Denham (2018)
Capsule Independent Uptake of the Fungal Pathogen Cryptococcus neoformans into Brain Microvascular Endothelial Cells
Wilber Sabiiti (2012)
Virulence factors of medically important fungi.
L. H. Hogan (1996)
Mechanisms of infection by the human fungal pathogen Cryptococcus neoformans.
Wilber Sabiiti (2012)
Induction of Capsule Growth in Cryptococcus neoformans by Mammalian Serum and CO2
Oscar Zaragoza (2003)
A Soluble Mannose Receptor Immunoadhesin Enhances Phagocytosis of Pneumocystis carinii by Human Polymorphonuclear Leukocytes In Vitro
Stehle (2000)
Experimental Pulmonary Cryptococcus neoformans Infection in Rats
D. Goldman (1999)
Pathogenesis of pulmonary Cryptococcus neoformans infection in the rat.
D. Goldman (1994)
Granulocyte-colony stimulating factor facilitates the restoration of resistance to opportunistic fungi in leukopenic mice.
K. Uchida (1992)
Induction of interleukin-6 mRNA in rat alveolar macrophages by in vitro exposure to both Cryptococcus neoformans and anti-C. neoformans antiserum.
R. K. Li (1997)
Cryptococcosis in the era of AIDS--100 years after the discovery of Cryptococcus neoformans.
T. Mitchell (1995)
Evolutionarily conserved recognition and innate immunity to fungal pathogens by the scavenger receptors SCARF1 and CD36
T. Means (2009)
Animal Models of Cryptococcus neoformans in Identifying Immune Parameters Associated With Primary Infection and Reactivation of Latent Infection
Tyler G Normile (2020)
Binding of unopsonized Cryptococcus neoformans by human bronchoalveolar macrophages: inhibition by a large-molecular-size serum component.
S. Levitz (1992)
Experimental and calculated parameters on particle phagocytosis by alveolar macrophages.
P. Camner (2002)
Mannose receptor and phagocytosis
I. Fraser (1999)
Glucuronoxylomannan from Cryptococcus neoformans Down-regulates the Enzyme 6-Phosphofructo-1-kinase of Macrophages*
J. Grechi (2011)
Mononuclear phagocyte-mediated antifungal immunity: the role of chemotactic receptors and ligands
Muthulekha Swamydas (2015)
Conditions affecting the adherence of Cryptococcus neoformans to rat glial and lung cells in vitro.
G. Merkel (1993)
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