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Identification And Molecular Characterization Of Adenovirus Types (HAdV-8, HAdV-37, HAdV-4, HAdV-3) In An Epidemic Of Keratoconjunctivitis Occurred In Pune, Maharashtra, Western India.

V. Gopalkrishna, N. Ganorkar, P. R. Patil
Published 2016 · Medicine, Biology

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Epidemic keratoconjunctivitis (EKC) is a highly contagious infectious disease of the ocular surface and is caused mainly due to adenoviruses species D, B, and E. The present study was carried out to identify and characterize the viral etiological agents associated with the keratoconjunctivitis cases reported from Pune (Maharashtra), Western India between November-December 2013 and January, October-November 2014. Conjunctival swab specimens (n = 23) obtained from keratoconjunctivitis patients were subjected to detection of Adenovirus (AdV) and Enterovirus (EV) by PCR/RT-PCR using hexon and 5' NCR gene specific primers, respectively. Molecular typing of AdV and EV positive specimens was carried out by amplifying penton, fiber, and VP1 genes, respectively followed by sequencing and phylogenetic analysis. In this study, human adenovirus (HAdV) was identified as an etiological agent. None of the clinical specimens were found positive for enterovirus. AdV positivity in keratoconjunctivitis cases was found to be 60.9% (14/23). Fourteen of the HAdV positive strains, all of them were amplified by hexon gene, nine strains by fiber gene, and all 14 strains by penton gene specific primers. Sequencing of all HAdV positive samples revealed the presence of HAdV-8, HAdV-37, HAdV-3, and HAdV-4. All Indian strains showed highest nucleotide identity with the reference strains reported worldwide. The study revealed the circulation of HAdV-8 (78.6%) as predominant AdV strain followed by HAdV-37, HAdV-3, and HAdV-4 (7.2%) identified in the epidemic keratoconjunctivitis. Multiple types of AdVs in EKC reported for the first time in Western India. J. Med. Virol. 88:2100-2105, 2016. © 2016 Wiley Periodicals, Inc.
This paper references
10.1017/S0022172400064810
A large outbreak of keratoconjunctivitis due to adenovirus type 8.
S. Richmond (1984)
Outbreak of epidemic keratoconjunctivitis caused by adenovirus type 54 in a nursery school in Kobe City, Japan in 2008.
K. Akiyoshi (2011)
10.1093/oxfordjournals.aje.a112890
An outbreak of adenovirus type 3 disease at a private recreation center swimming pool.
W. Martone (1980)
10.1097/00004397-200201000-00008
A Twenty-One Year Surveillance of Adenoviral Conjunctivitis in Sapporo, Japan
K. Aoki (2002)
Molecular epidemiology of adenoviral keratoconjunctivitis in Saudi Arabia
K. Tabbara (2010)
10.3238/arztebl.2011.0475
Epidemic keratoconjunctivitis: the current situation and recommendations for prevention and treatment.
B. Meyer-Rüsenberg (2011)
Isolation of a variant human adenovirus identified based on phylogenetic analysis during an outbreak of acute keratoconjunctivitis in Chennai
M.K. Janani (2012)
10.1016/J.OFTAL.2012.07.007
Queratoconjuntivitis adenovíricas: actualización
Julio J González-López (2013)
10.7883/YOKEN.65.260
A molecular epidemiologic study of human adenovirus type 8 isolates causing epidemic keratoconjunctivitis in Kawasaki City, Japan in 2011.
Tsuguto Fujimoto (2012)
10.1016/S0035-9203(97)90216-9
Recurrence of epidemic conjunctivitis caused by enterovirus-70 in Pune, India.
S. Gogate (1997)
10.3201/eid1502.080865
Enteroviruses in Patients with Acute Encephalitis, Uttar Pradesh, India
G. Sapkal (2009)
10.25646/669
Increasing case numbers of adenovirus conjunctivitis in Germany, 2010.
C. Adlhoch (2010)
10.1093/infdis/147.2.191
Keratoconjunctivitis associated with adenovirus type 37: an extended outbreak in an ophthalmologist's office.
R. Keenlyside (1983)
10.1128/JCM.00542-06
Sensitive, Seminested PCR Amplification of VP1 Sequences for Direct Identification of All Enterovirus Serotypes from Original Clinical Specimens
W. A. Nix (2006)
10.1556/EuJMI.4.2014.1.2
Human adenovirus: Viral pathogen with increasing importance.
B. Ghebremedhin (2014)
10.1128/JCM.37.5.1288-1293.1999
Typing of Human Enteroviruses by Partial Sequencing of VP1
M. Oberste (1999)
10.1016/j.oftal.2012.07.007
Adenoviral keratoconjunctivitis: an update.
J. J. González-López (2013)
10.1002/jmv.22252
Surveillance of adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 1995-2010.
Masako Nakamura (2012)
10.1017/S0950268815003246
Virology and epidemiology analyses of global adenovirus-associated conjunctivitis outbreaks, 1953-2013.
L. Zhang (2016)
10.1002/JMV.20886
Outbreak of acute hemorrhagic conjunctivitis in Maharashtra and Gujarat states of India, caused by Coxsackie virus A-24 variant.
V. Gopalkrishna (2007)
10.1093/molbev/msr121
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
K. Tamura (2011)
10.1093/infdis/140.1.42
Pharyngoconjunctival fever caused by adenovirus type 4: report of a swimming pool-related outbreak with recovery of virus from pool water.
L. D'angelo (1979)
10.1016/S0161-6420(93)31389-8
Prolonged recovery of desiccated adenoviral serotypes 5, 8, and 19 from plastic and metal surfaces in vitro.
Y. J. Gordon (1993)
10.1128/JCM.43.2.726-732.2005
Five New Genome Types of Adenovirus Type 37 Caused Epidemic Keratoconjunctivitis in Sapporo, Japan, for More Than 10 Years
T. Ariga (2005)
10.1093/infdis/132.2.142
Epidemic keratoconjunctivitis: a community outbreak of mixed adenovirus type 8 and type 19 infection.
B. Guyer (1975)
Outbreak of epidemic keratoconjunctivitis caused by adenovirus in medical residents
C. Meléndez (2009)
10.1128/JCM.39.2.498-505.2001
Rapid Typing of Human Adenoviruses by a General PCR Combined with Restriction Endonuclease Analysis
A. Allard (2001)
10.1002/JMV.2013
Molecular characterization of human enteroviruses in clinical samples: comparison between VP2, VP1, and RNA polymerase regions using RT nested PCR assays and direct sequencing of products.
I. Casas (2001)
Adenoviruses
Wold WSM (2013)
10.3129/i10-010
Public Health adds value to an investigation of epidemic keratoconjunctivitis.
Silvina C Mema (2010)
10.1002/JMV.21045
A community-derived outbreak of adenovirus type 3 in children in Taiwan between 2004 and 2005.
Sui-Yuan Chang (2008)



This paper is referenced by
10.3855/jidc.12093
Molecular characterization of adenovirus from an ongoingmulti-centric keratoconjunctivitis study in India.
Ferdinamarie Sharmila Philomenadin (2020)
10.1080/08820538.2019.1620800
A Review of Next-Generation Sequencing (NGS): Applications to the Diagnosis of Ocular Infectious Diseases
Lina Ma (2019)
10.1016/j.vaccine.2018.02.050
A recombinant trivalent vaccine candidate against human adenovirus types 3, 7, and 55.
T. Liu (2018)
10.4103/ijmm.IJMM_17_258
Molecular epidemiology of circulating human adenovirus types in acute conjunctivitis cases in Chandigarh, North India
Mini P Singh (2018)
10.3390/v11030242
Decoy Receptor Interactions as Novel Drug Targets against EKC-Causing Human Adenovirus
N. Chandra (2019)
10.3389/fcimb.2019.00417
A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7
Rong Wang (2019)
10.1038/s41426-018-0206-y
Seroepidemiological investigation of HAdV-4 infection among healthy adults in China and in Sierra Leone, West Africa
B. Wang (2018)
10.1016/j.virol.2020.04.005
Human mastadenovirus-B (HAdV-B)-specific E3-CR1β and E3-CR1γ glycoproteins interact with each other and localize at the plasma membrane of non-polarized airway epithelial cells
Poornima Kotha Lakshmi Narayan (2020)
10.7883/yoken.JJID.2017.349
Epidemic Keratoconjunctivitis Cases Resulting from Adenovirus Types 8 and 54 Detected at Fukuoka University Hospital between 2014 and 2015.
Tomoko Tsukahara-Kawamura (2018)
10.1186/s12879-019-4232-8
An outbreak of epidemic keratoconjunctivitis caused by human adenovirus type 8 in primary school, southwest China
Duo Li (2019)
10.3390/v11050395
Sialic Acid-Containing Glycans as Cellular Receptors for Ocular Human Adenoviruses: Implications for Tropism and Treatment
N. Chandra (2019)
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