Online citations, reference lists, and bibliographies.
← Back to Search

Subchondral Bone Fragility With Meniscal Tear Accelerates And Parathyroid Hormone Decelerates Articular Cartilage Degeneration In Rat Osteoarthritis Model

Yugo Morita, H. Ito, Masahiro Ishikawa, T. Fujii, M. Furu, M. Azukizawa, A. Okahata, T. Tomizawa, S. Kuriyama, S. Nakamura, K. Nishitani, H. Yoshitomi, S. Matsuda
Published 2018 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The aims of this study were to investigate the influence of subchondral bone fragility (SBF) on the progression of the knee osteoarthritis by using a novel rat model, and to examine the preventive effect of parathyroid hormone (PTH) on cartilage degeneration. First, 40 rats were assigned to the following four groups: Sham, SBF, Medial meniscal tear (MMT), and MMT + SBF groups. In SBF and MMT + SBF groups, we induced SBF by microdrilling the subchondral bone. Second, 10 additional rats were randomly assigned to the following two groups: MMT + SBF + saline and MMT + SBF + PTH groups. Osteoarthritic changes in the articular cartilage and subchondral bone were evaluated using safranin‐O/fast green staining, matrix metalloproteinase‐13 (MMP‐13), and type X collagen immunohistochemistry, toluidine blue staining, and micro‐CT scanning. The combination of SBF and meniscal tear increased the number of mast cells in the subchondral bone, and led to the abnormal subchondral bone microarchitecture, such as abnormally decreased trabecular number and increased trabecular thickness, compared with meniscal tear alone. Moreover, SBF with meniscal tear enhanced articular cartilage degeneration and increased the expression of MMP‐13 and type X collagen, compared with meniscal tear alone. The administration of PTH decreased the number of mast cells in the subchondral bone and improved the microstructural parameters of the subchondral bone, and delayed the progression of articular cartilage degeneration. These results suggest that SBF is one of the factors underlying the osteoarthritis development, especially in knees with traumatic osteoarthritis, and that the administration of PTH is a potential therapeutic treatment for preventing OA progression. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1959–1968, 2018.
This paper references
10.1016/j.jbiomech.2009.07.022
Computational biomechanics of articular cartilage of human knee joint: effect of osteochondral defects.
R. Shirazi (2009)
10.1177/036354658601400405
Meniscal tears: The effect of meniscectomy and of repair on intraarticular contact areas and stress in the human knee
M. Baratz (1986)
Acce pt e d Artic le 38
10.1186/ar3103
Subchondral bone microstructural damage by increased remodelling aggravates experimental osteoarthritis preceded by osteoporosis
M. Bellido (2010)
10.1111/j.1600-065X.2007.00506.x
Synovial mast cells: role in acute and chronic arthritis
P. Nigrovic (2007)
10.1080/13697130701857639
An update review of cellular mechanisms conferring the indirect and direct effects of estrogen on articular cartilage
L. Tankó (2008)
changes in the rat anterior cruciate ligament transection and meniscec - tomized models of osteoarthritis
H Aagaard (1999)
10.1038/aps.2012.28
Efficacy of zoledronic acid in treatment of teoarthritis is dependent on the disease progression stage in rat medial meniscal tear model
Degang Yu (2012)
Acce pt e d Artic le
10.1002/jbmr.1765
PTH‐enhanced structural allograft healing is associated with decreased angiopoietin‐2–mediated arteriogenesis, mast cell accumulation, and fibrosis
Robinder S. Dhillon (2013)
10.1038/nm.2146
Transcriptional regulation of endochondral ossification by HIF-2α during skeletal growth and osteoarthritis development
T. Saito (2010)
10.1007/s00223-015-9998-0
Three-Times-Weekly Administration of Teriparatide Improves Vertebral and Peripheral Bone Density, Microarchitecture, and Mechanical Properties Without Accelerating Bone Resorption in Ovariectomized Rats
R. Takao-Kawabata (2015)
10.1126/science.306.5705.2164b
Of Mice . . .
S. Simpson (2004)
10.1210/jc.2011-3479
Randomized Teriparatide [human parathyroid hormone (PTH) 1-34] Once-Weekly Efficacy Research (TOWER) trial for examining the reduction in new vertebral fractures in subjects with primary osteoporosis and high fracture risk.
T. Nakamura (2012)
10.1016/j.joca.2016.01.006
Exercise intervention increases expression of bone morphogenetic proteins and prevents the progression of cartilage-subchondral bone lesions in a post-traumatic rat knee model.
H. Iijima (2016)
10.1016/j.joca.2015.04.015
Effects of short-term gentle treadmill walking on subchondral bone in a rat model of instability-induced osteoarthritis.
H. Iijima (2015)
Effects of short-term gentle treadmill walking on subchondral bone in a rat model of instability-induced osteoarthritis. Osteoarthritis Cartilage 23:1563–1574
H Iijima (2015)
10.1136/annrheumdis-2012-202231
Efficacy and safety of strontium ranelate in the treatment of knee osteoarthritis: results of a double-blind, randomised placebo-controlled trial
J. Reginster (2013)
10.1016/j.joca.2014.05.009
Destabilization of the medial meniscus leads to subchondral bone defects and site-specific cartilage degeneration in an experimental rat model.
H. Iijima (2014)
10.1002/ART.20124
The role of subchondral bone remodeling in osteoarthritis: reduction of cartilage degeneration and prevention of osteophyte formation by alendronate in the rat anterior cruciate ligament transection model.
T. Hayami (2004)
10.3899/jrheum.110777
Aggravation of ADAMTS and Matrix Metalloproteinase Production and Role of ERK1/2 Pathway in the Interaction of Osteoarthritic Subchondral Bone Osteoblasts and Articular Cartilage Chondrocytes — Possible Pathogenic Role in Osteoarthritis
I. Prasadam (2012)
10.1016/j.joca.2010.05.030
The OARSI histopathology initiative - recommendations for histological assessments of osteoarthritis in the rat.
N. Gerwin (2010)
10.1002/jbmr.3178
Teriparatide Treatment Improves Bone Defect Healing Via Anabolic Effects on New Bone Formation and Non‐Anabolic Effects on Inhibition of Mast Cells in a Murine Cranial Window Model
L. Zhang (2017)
10.1186/1471-2474-9-20
A role for subchondral bone changes in the process of osteoarthritis; a micro-CT study of two canine models
Y. H. Sniekers (2008)
10.1038/aps.2012.167
Strontium ranelate reduces cartilage degeneration and subchondral bone remodeling in rat osteoarthritis model
Degang Yu (2013)
10.1053/JOCA.2002.0823
Induction of osteoarthritis in the rat by surgical tear of the meniscus: Inhibition of joint damage by a matrix metalloproteinase inhibitor.
M. J. Janusz (2002)
10.1177/00220345450240030501
Formic Acid-Sodium Citrate Decalcification and Butyl Alcohol Dehydration of Teeth and Bones for Sectioning in Paraffin
A. Morse (1945)
10.1016/j.bone.2010.11.010
Subchondral cysts create increased intra-osseous stress in early knee OA: A finite element analysis using simulated lesions.
D. McErlain (2011)
10.1007/978-1-4471-6578-1_5
Grading and Staging
Y. Park (2015)
10.1002/jor.21394
Chondroprotective effect of alendronate in a rabbit model of osteoarthritis
T. Shirai (2011)
Destabilization of the medial meniscus leads to subchondral bone defects and sitespecific cartilage degeneration in an experimental rat model. Osteoarthritis Cartilage 22:1036–1043
H Iijima (2014)
10.1172/JCI6610
Increased bone formation by prevention of osteoblast apoptosis with parathyroid hormone.
R. Jilka (1999)
10.1136/ard.2009.121681
Meniscal pathology on MRI increases the risk for both incident and enlarging subchondral bone marrow lesions of the knee: the MOST Study
M. Englund (2010)
Regulation of cyclooxygenase-2 expression in bovine chondrocytes in culture by interleukin 1alpha, tumor necrosis factor-alpha, glucocorticoids, and 17beta-estradiol.
S. Morisset (1998)
10.1111/j.1600-0838.1999.tb00443.x
Function of the normal meniscus and consequences of meniscal resection
H. Aagaard (1999)
Endochondral ossification signals in cartilage degradation during osteoarthritis progression in experimental mouse models.
H. Kawaguchi (2008)
10.1016/j.bone.2008.04.012
Mechanical loading enhances the anabolic effects of intermittent parathyroid hormone (1-34) on trabecular and cortical bone in mice.
T. Sugiyama (2008)
10.1093/rheumatology/kem323
17Beta-oestradiol up-regulates the expression of a functional UDP-glucose dehydrogenase in articular chondrocytes: comparison with effects of cytokines and growth factors.
L. Maneix (2008)
10.1016/j.joca.2015.11.011
Characterization of synovial mast cells in knee osteoarthritis: association with clinical parameters.
B. D. de Lange-Brokaar (2016)
10.1002/ART.21724
The association of meniscal pathologic changes with cartilage loss in symptomatic knee osteoarthritis.
D. Hunter (2006)
10.1002/jbmr.2460
Spatiotemporal Analyses of Osteogenesis and Angiogenesis via Intravital Imaging in Cranial Bone Defect Repair
Chunlan Huang (2015)
10.1186/ar3209
Bone marrow lesions predict site-specific cartilage defect development and volume loss: a prospective study in older adults
D. Doré (2010)
10.1186/ar1716
Effect of risedronate on joint structure and symptoms of knee osteoarthritis: results of the BRISK randomized, controlled trial [ISRCTN01928173]
T. Spector (2005)
10.1016/j.joca.2015.05.018
Joint distraction attenuates osteoarthritis by reducing secondary inflammation, cartilage degeneration and subchondral bone aberrant change.
Y. Chen (2015)
10.1196/annals.1346.002
PTHrP and Skeletal Development
H. Kronenberg (2006)
10.1097/00004424-200010000-00004
Subchondral Bone and Cartilage Disease: A Rediscovered Functional Unit
H. Imhof (2000)
10.1016/j.jim.2008.07.013
Improved methods for immunohistochemical detection of BrdU in hard tissue.
A. Shimada (2008)
10.1186/1471-2474-11-185
The influence of long-term treadmill exercise on bone mass and articular cartilage in ovariectomized rats
Ting-Kuo Chang (2010)
10.1016/J.JOCA.2005.07.014
Osteoarthritis cartilage histopathology: grading and staging.
K. Pritzker (2006)
10.1002/ART.22041
Contribution of runt-related transcription factor 2 to the pathogenesis of osteoarthritis in mice after induction of knee joint instability.
S. Kamekura (2006)
10.1016/j.bcp.2011.09.018
Subchondral bone as a key target for osteoarthritis treatment.
S. Castañeda (2012)
10.1002/art.24243
Identification of the core element responsive to runt-related transcription factor 2 in the promoter of human type X collagen gene.
A. Higashikawa (2009)
10.1016/j.joca.2015.07.012
Bone turnover and articular cartilage differences localized to subchondral cysts in knees with advanced osteoarthritis.
Y. Chen (2015)
10.1016/j.joca.2014.07.013
Parathyroid hormone (1-34) prevents cartilage degradation and preserves subchondral bone micro-architecture in guinea pigs with spontaneous osteoarthritis.
J. Yan (2014)
10.1056/NEJMRA067395
Mechanisms of anabolic therapies for osteoporosis.
E. Canalis (2007)
10.1016/J.BONE.2005.08.007
Characterization of articular cartilage and subchondral bone changes in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis.
T. Hayami (2006)
Efficacy and safety of strontium ranelate in the treatment of knee osteoarthritis: results of a double-blind, randomised placebocontrolled trial
JY Reginster (2013)
10.1016/j.joca.2011.07.003
Improving subchondral bone integrity reduces progression of cartilage damage in experimental osteoarthritis preceded by osteoporosis.
M. Bellido (2011)
10.1016/J.JOCA.2005.03.004
Osteoarthritis development in novel experimental mouse models induced by knee joint instability.
S. Kamekura (2005)
10.1016/j.joca.2016.01.007
Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized guinea pigs.
M-W Dai (2016)
Characteriza - tion of articular cartilage and subchondral
T Hayami (2006)
10.1126/scitranslmed.3002214
Teriparatide as a Chondroregenerative Therapy for Injury-Induced Osteoarthritis
E. Sampson (2011)
10.1001/ARCHINTE.165.15.1762
Opposite bone remodeling effects of teriparatide and alendronate in increasing bone mass.
M. Mcclung (2005)
10.1093/RHEUMATOLOGY/32.8.671
Mast cells in the synovium and synovial fluid in osteoarthritis.
G. Dean (1993)
10.1016/j.berh.2014.01.004
The epidemiology of osteoarthritis.
V. L. Johnson (2014)
17 Beta - oestradiol upregulates the expression of a functional UDP - glucose dehydrogenase in articular chondrocytes : comparison with effects of cytokines and growth factors
L Maneix (2008)
10.1016/J.JOCA.2007.07.007
Meniscal damage associated with increased local subchondral bone mineral density: a Framingham study.
G. Lo (2008)
10.1007/s00198-013-2305-x
Increased osteoblast and osteoclast indices in individuals with systemic mastocytosis
S. Seitz (2013)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar