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DOI: 10.1002/jssc.201100919

A New Solid-phase Extraction And HPLC Method For Determination Of Patulin In Apple Products And Hawthorn Juice In China.

Yuchun Zhou, Wei-jun Kong, Y. Li, A. Logrieco, Jun Xu, Meihua Yang
Published 2012 · Medicine, Chemistry

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A new solid-phase extraction (SPE) pretreatment method using a home-made polyvinylpolypyrrolidone-florisil (PVPP-F) column was developed for the analysis of patulin in apple and hawthorn products in China. Fifty samples (25 apple juices, 12 apple jams, and 13 hawthorn juices) were prepared using the new method and then analyzed by high performance liquid chromatography with diode array detection (HPLC-DAD) on an Agela Venusil MP C(18) reversed-phase column (4.6 mm × 250 mm, 5 μm). The cleanup results for all samples using home-made PVPP-F column were compared with those obtained using a MycoSep®228 AflaPat column. The correlation coefficient R (0.9998) fulfilled the requirement of linearity for patulin in the concentration range of 2.5-250 μg/kg. The limits of detection (LODs) and quantification (LOQs) of patulin were 3.99 and 9.64 μg/kg for PVPP-F column, and 3.56 and 8.07 μg/kg for MycoSep®228 AflaPat column, respectively. Samples were spiked with patulin at levels ranging from 25 to 250 μg/kg, and recoveries using PVPP-F and MycoSep®228 AflaPat columns were in the range of 81.9-100.9% and 86.4-103.9%, respectively. Naturally occurring patulin was found in 2 of 25 apple juice samples (8.0%) and 1 of 13 hawthorn juice samples (7.7%) at concentrations ranging from 12.26 to 36.81 μg/kg. The positive results were further confirmed by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS).
This paper references
10.1016/j.fct.2008.02.027
Development and validation of a microemulsion electrokinetic chromatography method for patulin quantification in commercial apple juice.
M. Murillo-Arbizu (2008)
10.1021/JF0513157
Parallel synthesis: a new approach for developing analytical internal standards. Application to the analysis of patulin by gas chromatography-mass spectrometry.
M. Llovera (2005)
10.1016/J.ACA.2005.04.017
Liquid chromatographic method for the determination of patulin in apple juice using solid-phase extraction
V. Gökmen (2005)
10.1016/J.FOODCHEM.2008.11.074
Determination of patulin in apple and quince products by GC-MS using 13C5-7 patulin as internal standard.
S. Cunha (2009)
10.1021/JF049264L
Development of liquid chromatography-electrospray mass spectrometry for the determination of patulin in apple juice: investigation of its contamination levels in Japan.
R. Ito (2004)
10.1016/J.JFCA.2008.05.010
Application of matrix solid-phase dispersion–HPLC method to determine patulin in apple and apple juice concentrate
R. Wu (2008)
10.1093/JAOAC/90.1.167
Determination of patulin in apple and hawthorn beverages by solid-phase filtration column and liquid chromatography.
Fengqin Li (2007)
10.1007/S002170100354
Effects of various clarification treatments on patulin, phenolic compound and organic acid compositions of apple juice
V. Gökmen (2001)
10.1016/S0021-9673(01)88867-1
Release of 2-aminofluorene from N-(deoxyguanosin-8-yl)-2-aminofluorene by hydrazinolysis.
J. Bakthavachalam (1991)
10.1016/J.FOODCONT.2005.12.014
Solid-phase extraction and HPLC determination of patulin in apple juice concentrate
Jianke Li (2007)
10.1021/AC0618526
A new competitive fluorescence assay for the detection of patulin toxin.
M. de Champdoré (2007)
10.1016/J.FOODCONT.2004.06.020
Analysis of patulin in dutch food, an evaluation of a SPE based method
G. Boonzaaijer (2005)
10.1016/0021-9673(95)00861-6
Rapid reversed-phase liquid chromatographic determination of patulin in apple juice.
V. Gökmen (1996)
10.1016/S0956-7135(00)00023-2
Patulin in South African commercial apple products
N. Leggott (2001)
10.1016/j.chroma.2009.03.017
Determination of patulin in fruit juice and dried fruit samples by in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry.
Hiroyuki Kataoka (2009)
10.1016/S0021-9673(01)88866-X
Determination of patulin by capillary gas chromatography of the heptafluorobutyrate derivative.
E. Tarter (1991)
10.1016/J.FOODCONT.2008.05.010
Determination of patulin in solid and semisolid apple and pear products marketed in Argentina
Gustavo J. Funes (2009)
10.1016/J.FOODCHEM.2008.07.054
Occurrence of patulin and its dietary intake through apple juice consumption by the Spanish population.
M. Murillo-Arbizu (2009)
10.1016/S0021-9673(99)01341-2
Chromatographic determination of the mycotoxin patulin in fruit and fruit juices.
G. Shephard (2000)



This paper is referenced by
10.1002/jssc.201300885
Simultaneous determination of four aflatoxins and ochratoxin A in ginger and related products by HPLC with fluorescence detection after immunoaffinity column clean-up and postcolumn photochemical derivatization.
J. Wen (2013)
10.1155/2016/8104838
Polyvinylpolypyrrolidone Supported Brønsted Acidic Catalyst for Esterification
Song Wang (2016)
10.1016/S1876-3553(13)60011-3
Latest Advancement for Detection Methods of Mycotoxins in Traditional Chinese Medicine
Feng Xu (2012)
10.1016/j.fct.2018.06.022
Investigation of patulin and citrinin in grape must and wine from grapes naturally contaminated by strains of Penicillium expansum.
Vladimír Ostrý (2018)
10.1007/978-3-319-92850-0
Mycotoxins in Plants and Plant Products: Cocoa, Coffee, Fruits and Fruit Products, Medicinal Plants, Nuts, Spices, Wine
Martin Weidenbörner (2018)
10.1080/19440049.2017.1419580
Patulin in fruit juices: occurrence, bioaccessibility, and risk assessment for Serbian population
Ljilja D Torović (2018)
10.1039/C5AY03099D
PRiME pass-through cleanup for the fast determination of aflatoxins in human serum by using LC-MS/MS
Zhi-yong Chen (2016)
10.1002/jssc.201400922
Preparation of 4-butylaniline-bonded silica gel for the solid-phase extraction of flavone glycosides.
Ganghui Chu (2015)
10.1016/j.toxicon.2020.05.006
Progress in the distribution, toxicity, control, and detoxification of patulin: A review.
Chaozhi Wei (2020)
Detection of Patulin Mycotoxin in Dietary Supplements and Herbal Blends Containing Hawberry from Crataegus spp
Dary natury (2019)
10.1016/B978-0-12-411471-5.00021-1
Mycotoxin Analytical Methods
Magdalena Twarużek (2016)
10.1002/9781118992685.CH3
Review of Sample Treatments and the State‐of‐the‐art of Analytical Techniques for Mycotoxins in Food
Natalia Arroyo-Manzanares (2017)
C 2 H 2 Transcription Factor brlA Regulating Conidiation and Affecting Growth and Biosynthesis of Secondary Metabolites in Aspergillus clavatus
Xiaofei Han (2018)
10.1007/s00216-015-8705-3
An optimized method for the accurate determination of patulin in apple products by isotope dilution-liquid chromatography/mass spectrometry
Miyeong Seo (2015)
Identification and characterisation of spoilage moulds in chocolate confectionery and patulin-producing moulds in apples
N. Clercq (2016)
10.1016/j.foodchem.2013.09.005
Occurrence of toxigenic fungi and determination of mycotoxins by HPLC-FLD in functional foods and spices in China markets.
Weijun Kong (2014)
10.1016/j.aca.2013.08.042
Development of a sensitive and reliable high performance liquid chromatography method with fluorescence detection for high-throughput analysis of multi-class mycotoxins in Coix seed.
Wei-jun Kong (2013)
10.1155/2019/2159097
Advantageous Extraction, Cleanup, and UHPLC-MS/MS Detection of Patulin Mycotoxin in Dietary Supplements and Herbal Blends Containing Hawberry from Crataegus spp.
A. Przybylska (2019)
10.1016/j.foodchem.2019.02.099
One step rapid dispersive liquid-liquid micro-extraction with in-situ derivatization for determination of aflatoxins in vegetable oils based on high performance liquid chromatography fluorescence detection.
Nan Wang (2019)
10.1007/s12161-018-1340-9
Developments in the Monitoring of Patulin in Fruits Using Liquid Chromatography: an Overview
Ilona Sadok (2018)
10.1021/jf501171w
Ionic liquid-based one-step micellar extraction of multiclass polar compounds from hawthorn fruits by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry.
Shuai-Shuai Hu (2014)
10.1177/0748233712457454
Patulin in apple juice and its risk assessments on albino mice
Mansour A. Al-Hazmi (2014)
10.3920/WMJ2012.1492
Developments in mycotoxin analysis: an update for 2011-2012
G. Shephard (2013)
10.1080/19440049.2016.1266394
Determination of patulin in apple juice using magnetic solid-phase extraction coupled with high-performance liquid chromatography
Youwei Yu (2017)
10.4315/0362-028X.JFP-16-319
A Comprehensive Review of Spirit Drink Safety Standards and Regulations from an International Perspective.
Xiao-Na Pang (2017)
10.1016/j.foodchem.2017.04.117
Determination of trace patulin in apple-based food matrices.
Xianjiang Li (2017)
10.1007/s12161-015-0190-y
Optimization and Validation of a Method Without Alkaline Clean-Up for Patulin Analysis on Apple Puree Agar Medium (APAM) and Apple Products
Nikki De Clercq (2015)
10.1080/21501203.2020.1727578
Occurrence and analysis of mycotoxins in domestic Chinese herbal medicines
Lu Qin (2020)
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