Novel Biopolymer Matrices For Microencapsulation Of Phages: Enhanced Protection Against Acidity And Protease Activity.
C. Dini, G. A. Islan, P. J. de Urraza, G. R. Castro
Published 2012 · Medicine, Chemistry
Download PDFAnalyze on Scholarcy
Phage therapy by oral administration requires enhanced resistance of phages to the harsh gastric conditions. The aim of this work is the microencapsulation of phages in natural biopolymeric matrices as a protective barrier against the gastric environment. Alginate and pectin are used as base polymers. Further emulsification with oleic acid or coating with a different biopolymer is also studied. Emulsified pectin shows the maximum encapsulation efficiency and the highest protection against acidity, leaving more than 10(3) active phages after 30 min exposure at pH = 1.6, and protects phage from pepsin activity (4.2 mg mL(-1)). Non-encapsulated phages are fully inactivated at pH = 1.6 or with pepsin (0.5 mg mL(-1)) after 10 min.
This paper references
Structure formation in sugar containing pectin gels – Influence of Ca2+ on the gelation of low-methoxylated pectin at acidic pH
H. Kastner (2012)
Network formation and degradation behavior of hydrogels formed by Michael-type addition reactions.
A. Metters (2005)
Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin‐producing E. coli(EHEC and STEC) in cattle
C. Dini (2010)
Chance and design--proton transfer in water, channels and bioenergetic proteins.
C. A. Wraight (2006)
What’s new in haemolytic uraemic syndrome?
S. Johnson (2008)
Triggered release of proteins from emulsan-alginate beads.
G. R. Castro (2005)
Present and Future Role of Chitin and Chitosan in Food
E. Agulló (2003)
Bacteriophages and their application in food safety.
P. García (2008)
Viruses vs. superbugs
T. Häusler (2006)
Reduction in Exopolysaccharide Viscosity as an Aid to Bacteriophage Penetration through Pseudomonas aeruginosa Biofilms
G. Hanlon (2001)
Enumeration of bacteriophages by double agar overlay plaque assay.
A. Kropinski (2009)
Controlled release biopolymers for enhancing the immune response.
G. R. Castro (2007)
Control Release Applications in Food Technology’’, in Advances in Bioprocesses in Food Industries
V. E. Bosio (2011)
Bacteriophages for prophylaxis and therapy in cattle, poultry and pigs.
R. Johnson (2008)
Prediction of the pH and the temperature-dependent swelling behavior of Ca2+-alginate hydrogels by artificial neural networks
M. Koc (2008)
Theoretical analysis of the release of slowly dissolving drugs from spherical matrix systems.
G. Frenning (2004)
Application of Bacteriophages To Control Intestinal Escherichia coli O157:H7 Levels in Ruminants
H. Sheng (2006)
Enhanced alginate microspheres as means of oral delivery of bacteriophage for reducing Staphylococcus aureus intestinal carriage
Y. Ma (2012)
Alginate hydrogels as biomaterials.
Alexander Augst (2006)
ESCHERICHIA COLI ENTEROHEMORRAGICA Y SINDROME UREMICO HEMOLITICO EN ARGENTINA
M. Rivero (2004)
Bacteriophage isolated from feedlot cattle can reduce Escherichia coli O157:H7 populations in ruminant gastrointestinal tracts.
T. Callaway (2008)
Microencapsulation of Bacteriophage Felix O1 into Chitosan-Alginate Microspheres for Oral Delivery
Yongsheng Ma (2008)
THE INFLUENCE OF SODIUM SALTS ON THE CLOSURE OF THE OESOPHAGEAL GROOVE IN CALVES.
R. F. Riek (1954)
Environmentally degradable bio-based polymeric blends and composites.
E. Chiellini (2004)
Veterinary medicine. A textbook of the diseases of cattle, sheep, pigs, goats and horses.
O. Radostits (1994)
Molecular basis of C(2+)-induced gelation in alginates and pectins: the egg-box model revisited.
I. Braccini (2001)
Effect of bacteriophage DC22 on Escherichia coli O157:H7 in an artificial rumen system (Rusitec) and inoculated sheep
S. Bach (2003)
Transient adenylate cyclase activation accompanies differentiation of Trypanosoma brucei from bloodstream to procyclic forms.
S. Rolin (1993)
KGaA, Weinheim www.MaterialsViews.com e numbers, use DOI for citation !! Novel Biopolymer Matrices for Microencapsulation of Phages: Enhanced Protection
P. Garcia (2008)
This paper is referenced by
Evaluation of storage conditions and efficiency of a novel microencapsulated Salmonella phage cocktail for controlling S. enteritidis and S. typhimurium in-vitro and in fresh foods.
K. Petsong (2019)
High precision microfluidic microencapsulation of bacteriophages for enteric delivery.
Gurinder K. Vinner (2018)
Development of biopolymer nanocomposite for silver nanoparticles and Ciprofloxacin controlled release.
G. A. Islan (2015)
Long-Term Preservation of Bacteriophage Antimicrobials Using Sugar Glasses
Vincent Leung (2017)
Production of Phage Therapeutics and Formulations: Innovative Approaches
Maia Merabishvili (2019)
Isolation and characterization of bacteriophages against Non O157 Shiga toxin-producing Escherichia coli and their application as biosensor for foodborne pathogen detection
Nada Alasiri (2016)
Thermal Stability of Encapsulated Listeria Bacteriophage and Its Efficacy Against Listeria monocytogenes in Ready-To-Eat Meats
Hanie Ahmadi (2017)
The protective effect of food matrices on Listeria lytic bacteriophage P100 application towards high pressure processing.
N. Komora (2018)
Applications of Salmonella bacteriophages in the food production chain
Kantiya Petsong (2015)
The factors affecting effectiveness of treatment in phages therapy
M. H. Ly-Chatain (2014)
Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy
Abdallah S. Abdelsattar (2019)
Rumen Virus Populations: Technological Advances Enhancing Current Understanding
R. A. Gilbert (2020)
Microencapsulation of nisin in alginate beads by vibrating technology: Preliminary investigation
Diamante Maresca (2016)
Bacteriophages as antimicrobials in food products: History, biology and application
H. Anany (2015)
Bacteriophage Biocontrol in Poultry
S. A. Jassim (2017)
POTENTIAL APPLICATION OF LYOPHILIZATION IN COMMERCIAL USE OF BACTERIOPHAGE PREPARATIONS IN VETERINARY MEDICINE
Aneta Skaradzińska (2018)
Micro e nanoencapsulação como estratégias de estabilização de entidades bioativas: proteínas, enzimas e bacteriófagos
Luís Carlos Araújo Freixo (2013)
Phage Therapy with a Focus on the Human Microbiota
Sharita Divya Ganeshan (2019)
Microencapsulation of Clostridium difficile specific bacteriophages using microfluidic glass capillary devices for colon delivery using pH triggered release
Gurinder K. Vinner (2017)
Bacteriophage Procurement for Therapeutic Purposes
Beata Weber-Dąbrowska (2016)
Engineering bacteriophage encapsulation processes to improve stability and controlled release using pH responsive formulations
Gurinder K. Vinner (2018)
Formulation and Evaluation of Multi-particulate Antibiotic Alternatives for Oral Delivery to Livestock Animals to Target Gut Pathogens
Yin-Hing Ma (2016)
Chitosan Nanoparticles as Carriers for the Delivery of ΦKAZ14 Bacteriophage for Oral Biological Control of Colibacillosis in Chickens
Kaikabo Adamu Ahmad (2016)
Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells.
Maximiliano L. Cacicedo (2016)
The Use of Bacteriophages in Animal Health and Food Protection
Katarzyna Kosznik-Kwaśnicka (2019)
Going viral: designing bioactive surfaces with bacteriophage.
Zeinab Hosseinidoust (2014)
Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy against Salmonella spp.
J. Colom (2015)
Nanotechnology-Based Packaging Materials for Fresh and Processed Meats
Fidel Toldrá (2017)
Bacteriophage-based weapons for the war against foodborne pathogens
Lawrence D. Goodridge (2018)
Characterization and Stability Analysis of Biopolymeric Matrices Designed for Phage-Controlled Release
Cecilia Dini (2014)
Bacteriophages as Weapons Against Bacterial Biofilms in the Food Industry
D. Gutiérrez (2016)
Formulation, stabilisation and encapsulation of bacteriophage for phage therapy.
D. Malik (2017)See more