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Development Of A Dual-Functional Hydrogel Using RGD And Anti-VEGF Aptamer.

N. Zhao, Mark R. Battig, M. Xu, X. Wang, N. Xiong, Y. Wang
Published 2017 · Chemistry, Medicine

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Synthetic molecular libraries hold great potential to advance the biomaterial development. However, little effort is made to integrate molecules with molecular recognition abilities selected from different libraries into a single biomolecular material. The purpose of this work is to incorporate peptides and nucleic acid aptamers into a porous hydrogel to develop a dual-functional biomaterial. The data show that an anti-integrin peptide can promote the attachment and growth of endothelial cells in a 3D porous poly(ethylene glycol) hydrogel and an antivascular endothelial growth factor aptamer can sequester and release VEGF of high bioactivity. Importantly, the dual-functional porous hydrogel enhances the growth and survival of endothelial cells. This work demonstrates that molecules selected from different synthetic libraries can be integrated into one system for the development of novel biomaterials.
This paper references
Nucleic Acids Res
L. L. Cummins (2019)
10.1016/j.biomaterials.2011.02.029
Advantages of RGD peptides for directing cell association with biomaterials.
S. Bellis (2011)
10.1016/j.biomaterials.2009.09.037
High throughput methods applied in biomaterial development and discovery.
A. Hook (2010)
10.1073/pnas.1520244113
Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles
M. Wang (2016)
10.1016/j.actbio.2015.07.016
Reprint of: Extracellular matrix as a biological scaffold material: Structure and function.
S. Badylak (2015)
10.1016/J.BIOMATERIALS.2003.09.084
The effect of hydrogel charge density on cell attachment.
G. Schneider (2004)
10.1083/JCB.114.5.1089
An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3- mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation
S. Massia (1991)
10.1021/ja305238a
Programmable release of multiple protein drugs from aptamer-functionalized hydrogels via nucleic acid hybridization.
Mark R. Battig (2012)
10.1016/J.BIOMATERIALS.2005.03.018
The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells.
F. Yang (2005)
10.1126/SCIENCE.2200121
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
C. Tuerk (1990)
10.1038/380548A0
Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2
P. Colas (1996)
10.1073/pnas.1312296111
Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes
Y. Ivarsson (2014)
10.1182/BLOOD.V96.12.3772.H8003772_3772_3778
Vascular endothelial growth factor binds to fibrinogen and fibrin and stimulates endothelial cell proliferation.
A. Sahni (2000)
10.1021/acs.biomac.5b00165
Aptamer-based polyvalent ligands for regulated cell attachment on the hydrogel surface.
Erin R. Gaddes (2015)
10.1126/sciadv.1600692
Protein engineering by highly parallel screening of computationally designed variants
Mark G F Sun (2016)
10.1016/j.actbio.2017.01.016
pH-sensitive peptide hydrogel for glucose-responsive insulin delivery.
Xia Li (2017)
10.1038/346818A0
In vitro selection of RNA molecules that bind specific ligands
A. Ellington (1990)
10.1021/acs.biomac.5b01511
Chimeric Aptamer-Gelatin Hydrogels as an Extracellular Matrix Mimic for Loading Cells and Growth Factors.
X. Zhang (2016)
10.1016/0092-8674(93)90231-E
Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment.
T. Wickham (1993)
10.1038/nrd3141
Aptamers as therapeutics
A. Keefe (2010)
Zwadlo- Klarwasser, Macromol
M. Bartneck (2014)
Bio­ technol
A. L. Hook (2012)
10.1016/S0142-9612(03)00343-0
RGD modified polymers: biomaterials for stimulated cell adhesion and beyond.
U. Hersel (2003)
10.1093/NAR/23.11.2019
Characterization of fully 2'-modified oligoribonucleotide hetero- and homoduplex hybridization and nuclease sensitivity.
L. Cummins (1995)
10.1016/J.BIOMATERIALS.2007.02.006
RGD-Functionalized polymer brushes as substrates for the integrin specific adhesion of human umbilical vein endothelial cells.
Stefano Tugulu (2007)
10.1038/nbt.2316
Combinatorial discovery of polymers resistant to bacterial attachment
A. Hook (2012)
10.1038/srep14297
A Drosera-bioinspired hydrogel for catching and killing cancer cells
Shihui Li (2015)
10.1074/jbc.273.32.20556
2′-Fluoropyrimidine RNA-based Aptamers to the 165-Amino Acid Form of Vascular Endothelial Growth Factor (VEGF165)
J. Ruckman (1998)
10.1002/mabi.201300362
The RGD coupling strategy determines the inflammatory response of human primary macrophages in vitro and angiogenesis in vivo.
M. Bartneck (2014)
10.1002/ADMA.200700949
High Throughput Surface Characterisation of a Combinatorial Material Library
A. J. Urquhart (2007)
10.1016/j.biomaterials.2011.10.062
Cell adhesion on an artificial extracellular matrix using aptamer-functionalized PEG hydrogels.
Niancao Chen (2012)
10.1021/bm501347s
Polymerization of affinity ligands on a surface for enhanced ligand display and cell binding.
E. Richards (2014)
10.1016/j.biomaterials.2012.07.044
A biomimetic lipid library for gene delivery through thiol-yne click chemistry.
L. Li (2012)
10.1080/09205063.2012.745714
Evaluation of RGD peptide hydrogel in the posterior segment of the rabbit eye
X. Wang (2013)
10.1021/bm100137q
Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds with tunable degradation and mechanical properties.
S. Zustiak (2010)
10.1016/j.biomaterials.2011.05.074
Affinity hydrogels for controlled protein release using nucleic acid aptamers and complementary oligonucleotides.
Boonchoy Soontornworajit (2011)
10.1111/j.1524-475X.2009.00466.x
Interactions between extracellular matrix and growth factors in wound healing
G. Schultz (2009)
10.1016/j.biomaterials.2014.06.001
Aptamer-functionalized superporous hydrogels for sequestration and release of growth factors regulated via molecular recognition.
Mark R. Battig (2014)
10.1002/jbm.a.35306
Peptide REDV-modified polysaccharide hydrogel with endothelial cell selectivity for the promotion of angiogenesis.
W. Wang (2015)
10.1002/ADFM.201103147
Cationic Hybrid Hydrogels from Amino‐Acid‐Based Poly(ester amide): Fabrication, Characterization, and Biological Properties
J. Wu (2012)
10.1038/NBT0395-265
Phage Libraries Displaying Cyclic Peptides with Different Ring Sizes: Ligand Specificities of the RGD-Directed Integrins
E. Koivunen (1995)
10.1007/s10456-009-9146-4
The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art
I. Arnaoutova (2009)
10.1038/natrevmats.2015.12
The design of reversible hydrogels to capture extracellular matrix dynamics.
A. Rosales (2016)
10.1016/J.BIOMATERIALS.2004.06.047
Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures.
Liora Almany (2005)
10.1016/S0142-9612(02)00176-X
Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering.
J. Burdick (2002)
10.1038/nbt1402
A combinatorial library of lipid-like materials for delivery of RNAi therapeutics
A. Akinc (2008)
10.1091/mbc.E09-07-0590
Binding to the Extracellular Matrix and Proteolytic Processing: Two Key Mechanisms Regulating Vascular Endothelial Growth Factor Action
N. Ferrara (2010)
10.1146/ANNUREV.CELLBIO.12.1.697
RGD and other recognition sequences for integrins.
E. Ruoslahti (1996)
10.1002/term.183
Initial evaluation of vascular ingrowth into superporous hydrogels
V. Keskar (2009)
10.1016/j.biomaterials.2011.02.025
The role of short synthetic adhesion peptides in regenerative medicine; the debate.
D. Williams (2011)
10.1016/S0142-9612(02)00175-8
Photopolymerizable hydrogels for tissue engineering applications.
K. Nguyen (2002)
10.1038/nmat2812
Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells
Ying Mei (2010)
10.1002/(SICI)1097-4636(199802)39:2<266::AID-JBM14>3.0.CO;2-B
Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing.
D. Hern (1998)
10.1091/MBC.4.12.1317
The vascular endothelial growth factor (VEGF) isoforms: differential deposition into the subepithelial extracellular matrix and bioactivity of extracellular matrix-bound VEGF.
J. Park (1993)
10.1002/mabi.201200315
High-throughput screening of substrate chemistry for embryonic stem cell attachment, expansion, and maintaining pluripotency.
M. R. Zonca (2013)
10.1074/jbc.271.23.13523
Identification of the Extracellular Matrix Binding Sites for Insulin-like Growth Factor-binding Protein 5*
A. Parker (1996)
10.1007/s11095-008-9801-2
PEG Hydrogels for the Controlled Release of Biomolecules in Regenerative Medicine
Chien-Chi Lin (2008)



This paper is referenced by
10.3390/cancers11111783
Targeting Integrins in Cancer Nanomedicine: Applications in Cancer Diagnosis and Therapy
Ping-Hsiu Wu (2019)
10.1002/mabi.202000337
Anti-VEGF-R2 Aptamer and RGD Peptide Synergize in a Bifunctional Hydrogel for Enhanced Angiogenic Potential.
T. Roy (2020)
10.3389/fbioe.2020.00665
Modulating Alginate Hydrogels for Improved Biological Performance as Cellular 3D Microenvironments
M. I. Neves (2020)
10.1007/s12274-018-2177-7
Plenty more room on the glass bottom: Surface functionalization and nanobiotechnology for cell isolation
A. Ansari (2018)
10.1016/j.biomaterials.2018.03.008
Programmable hydrogels.
Yong Wang (2018)
10.1002/mabi.201800353
Dynamic, Bioresponsive Hydrogels via Changes in DNA Aptamer Conformation.
S. W. Bae (2019)
10.1021/ACSBIOMATERIALS.9B00423
Macroporous Hydrogels for Stable Sequestration and Sustained Release of Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Using Nucleic Acid Aptamers.
Lidya Abune (2019)
10.1016/j.jconrel.2019.12.026
Development of hydrogel-like biomaterials via nanoparticle assembly and solid-hydrogel transformation.
J. Coyne (2019)
10.1002/adma.201806380
Biologically Inspired, Cell-Selective Release of Aptamer-Trapped Growth Factors by Traction Forces
Anna Stejskalová (2019)
10.3390/biomedicines8090307
Integrin-Targeting Peptides for the Design of Functional Cell-Responsive Biomaterials
Juping Zhao (2020)
10.1021/acsami.9b02462
Dual Aptamer-Functionalized in Situ Injectable Fibrin Hydrogel for Promotion of Angiogenesis via Codelivery of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor-BB.
N. Zhao (2019)
10.1002/adfm.201909011
Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior
Simão P. B. Teixeira (2020)
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