Online citations, reference lists, and bibliographies.

Effect Of PH On Gelatin Self-association Investigated By Laser Light Scattering And Atomic Force Microscopy

W. Lin, Lifeng Yan, C. Mu, W. Li, Mingrang Zhang, Qingshi Zhu
Published 2002 · Materials Science

Cite This
Download PDF
Analyze on Scholarcy
Share
The effect of pH on gelatin self-association in dilute solution has been investigated for the first time to the authors' knowledge by laser light scattering (LLS) and atomic force microscopy (AFM). It shows that, at the isoelectric point (IEP) of gelatin, the ampholyte macromolecules collapse due to electrostatic attraction forces of oppositely charged groups, and self-association occurs in turn by an intermolecular association process. The soluble aggregates are stabilized by ionic contacts, hydrogen bonds and hydrophobic interactions, and are protected from precipitation by hydrophilic groups on the surface of the particles. For gelatin-B away from the IEP, pH ≥ 6.0 or pH ≤ 4.0, the molecular chains behave more like random coils. As expected, the association–disassociation based mainly on electrostatic interactions appears reversible to some certain extent. The experimental results indicate that LLS and AFM, especially a combination of the two, play an important role in obtaining integrated and direct information on the structure and properties of this natural biopolymer. © 2002 Society of Chemical Industry
This paper references
The macromolecular chemistry of gelatin
A. Veis (1964)
10.1016/0032-3861(92)90284-4
Water soluble copolymers: 44. Ampholytic terpolymers of acrylamide with sodium 2-acrylamido-2-methylpropanesulphonate and 2-acrylamido-2-methylpropanetrimethyl-ammonium chloride
C. Mccormick (1992)
10.1021/ma00149a017
Hydrodynamic and thermodynamic behavior of short-chain polystyrene in toluene and cyclohexane at 34.5.degree.C
K. Huber (1985)
10.1016/0079-6107(94)90008-6
Biological applications of scanning probe microscopies.
V. Morris (1994)
10.1080/07366579008050910
SYNTHETIC POLYMERIC AMPHOLYTES IN SOLUTION
E. A. Bekturov (1990)
10.1080/00150198008209479
Phase changes in polyampholytes
S. Edwards (1980)
10.1021/ma00122a056
Observations of a circular, triple-helical polysaccharide using noncontact atomic force microscopy
T. McIntire (1995)
10.1007/978-3-642-78469-9_15
Protein-Polyelectrolyte Complexes
J. Xia (1994)
10.1002/polb.1995.090330602
Properties of an amphoteric cellulose derivative containing anionic carboxymethyl and cationic 2‐hydroxy‐3‐(trimethylammonio) propyl substituents
G. Zheng (1995)
10.1002/(SICI)1097-0282(199708)42:2<133::AID-BIP3>3.0.CO;2-O
Imaging of individual biopolymers and supramolecular assemblies using noncontact atomic force microscopy.
T. McIntire (1997)
10.1063/1.460012
Theory of polyampholyte solutions
P. Higgs (1991)
10.1021/ma00033a009
Water-Soluble Copolymers .43. Ampholytic Copolymers of Sodium 2-(Acrylamido)-2-Methylpropanesulfonate with [2-(Acrylamido)-2-Methylpropyl]trimethylammonium Chloride
C. Mccormick (1992)
10.1007/3-540-68384-4_3
Recent Advances in the Study of Synthetic Polyampholytes in Solutions
S. Kudaibergenov (1999)
The Science and technology of gelatin
A. Ward (1977)
10.1295/polymj.21.623
Simultaneous Frequency-Integrated and Incoherent-Elastic Light Scattering: Description of a New Photon-Correlation Spectrometer
E. Nordmeier (1989)
10.1103/PHYSREVLETT.83.4105
CLUSTERING INDUCED COLLAPSE OF A POLYMER BRUSH
T. Hu (1999)
10.1007/978-1-4757-4602-0
Biochemistry of collagen
G. N. Ramachandran (1976)
10.1021/ma00181a025
Water-soluble polymers. 28. Ampholytic copolymers of sodium 2-acrylamido-2-methylpropanesulfonate with (2-acrylamido-2-methylpropyl)dimethylammonium chloride: synthesis and characterization
C. Mccormick (1988)
10.1021/ma00128a056
Laser Light Scattering Study of the Phase Transition of Poly(N-isopropylacrylamide) in Water. 1. Single Chain
Chi Wu (1995)
10.1021/ma60068a022
Molecular Weight and Temperature Dependence of Polymer Dimensions in Solution
A. Akcasu (1979)



This paper is referenced by
10.1016/J.FOODHYD.2013.12.019
Molecular configuration of gelatin–water suspensions at low concentration
Paulo Díaz-Calderón (2014)
10.1007/s13197-016-2416-4
Effect of egg albumen protein addition on physicochemical properties and nanostructure of gelatin from fish skin
Luyun Cai (2016)
10.1111/1750-3841.14123
Structural Modification of Fish Gelatin by the Addition of Gellan, κ-Carrageenan, and Salts Mimics the Critical Physicochemical Properties of Pork Gelatin.
L. Sow (2018)
10.1016/j.carbpol.2017.06.001
Surface behavior and bulk properties of aqueous chitosan and type-B gelatin solutions for effective emulsion formulation.
Jagadish Chandra Roy (2017)
10.1016/S0921-0423(03)80005-9
Chapter V Collagen and gelatin
F. Wolf (2003)
10.1002/9781118165850.CH6
Ionic Mixed Interactions and Hofmeister Effects
Alberto Ciferri (2012)
10.1016/B978-0-12-385989-1.00006-5
A review of the application of atomic force microscopy (AFM) in food science and technology.
Shaoyang Liu (2011)
10.1002/MARC.200700162
Natural and Synthetic Polyampholytes, 1
A. Ciferri (2007)
10.1016/j.colsurfb.2012.02.041
Effect of aggregation behavior of gelatin in aqueous solution on the grafting density of gelatin modified with glycidol.
Jing Xu (2012)
10.1007/s11172-015-1070-8
Self-assembly and quantum chemical design of macrotricyclic and macrotetracyclic 3d-element metal chelates formed in the gelatin-immobilized matrix
Oleg V Mikhailov (2015)
10.1080/00958970701579282
Gelatin-immobilized metal complexes: synthesis and applications
O. Mikhailov (2008)
10.1016/J.ARABJC.2016.10.014
Molecular structure design and soft template synthesis of aza-, oxaaza- and thiaazamacrocyclic metal chelates in the gelatin matrix
O. Mikhailov (2017)
10.1016/J.FOODHYD.2007.10.007
Effects of alkaline and acid pretreatment on the physical properties and nanostructures of the gelatin from channel catfish skins
H. Yang (2008)
10.1111/J.1750-3841.2007.00480.X
Nanostructural characterization of catfish skin gelatin using atomic force microscopy.
H. Yang (2007)
10.1016/J.FOODHYD.2013.07.007
Rheology, texture and microstructure of gelatin gels with and without milk proteins
Zhihua Pang (2014)
10.1016/J.ICA.2012.07.037
Synthesis of 3d-element metalmacrocyclic chelates into polypeptide biopolymer medium and their molecular structures
Oleg V Mikhailov (2013)
10.1016/J.CEJ.2010.02.044
Effect of solution chemistry on flux decline during high concentration protein ultrafiltration through a hydrophilic membrane
Ying Pei Lim (2010)
10.3390/pharmaceutics3030440
The Influence of Formulation and Manufacturing Process Parameters on the Characteristics of Lyophilized Orally Disintegrating Tablets
Rhys James Jones (2011)
10.1145/3180382.3180403
The Drug Delivery System of Centella asiatica extract-loaded Gelatin Nanoparticles using of One-step desolvation Method
Kittithat Yongsirasawad (2018)
10.1515/revic-2017-0003
Polycyclic 3d-metalchelates formed owing to inner-sphere transmutations in the gelatin matrix: synthesis and structures
Oleg V Mikhailov (2017)
10.1016/J.FOODHYD.2008.04.016
Effects of concentration on nanostructural images and physical properties of gelatin from channel catfish skins
H. Yang (2009)
10.3402/nano.v5.21485
Molecular nanotechnologies of gelatin-immobilization using macrocyclic metal chelates
Oleg V Mikhailov (2014)
10.1515/revic-2018-0001
Electrophilic substitution in the d-metal hexacyanoferrate(II) gelatin-immobilized matrix systems
O. Mikhailov (2018)
10.1134/S1995078010010027
Self-assembly of molecules of metal macrocyclic compounds in nanoreactors on the basis of biopolymer-immobilized matrix systems
Oleg V Mikhailov (2010)
Formulation and process engineering of freeze-dried orally disintegrating tablets
Rhys Jones (2013)
10.1007/s10971-014-3468-4
Sol–gel technology and template synthesis in thin gelatin films
Oleg V Mikhailov (2014)
10.1016/j.scitotenv.2020.137391
Enhanced removal of chromium(III) for aqueous solution by EDTA modified attapulgite: Adsorption performance and mechanism.
Jiahong Wang (2020)
10.1016/J.CPLETT.2011.02.012
Hydrazine-induced thermo-reversible optical shifts in silver–gelatin bionanocomposites
C. Aimé (2011)
10.1021/bm400149a
Molar mass, entanglement, and associations of the biofilm polysaccharide of Staphylococcus epidermidis.
Mahesh Ganesan (2013)
10.1002/JSFA.2072
Isolation and characterization of collagen from bigeye snapper (Priacanthus macracanthus) skin
A. Jongjareonrak (2005)
10.3390/coatings9100595
Characterization and Testing of a Novel Sprayable Crosslinked Edible Coating Based on Salmon Gelatin
C. Char (2019)
Semantic Scholar Logo Some data provided by SemanticScholar