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Target-Triggered Assembly Of Nanogap Antennas To Enhance The Fluorescence Of Single Molecules And Their Application In MicroRNA Detection.

Manshu Peng, Feifei Sun, Na Na, J. Ouyang
Published 2020 · Materials Science, Medicine

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Nanogap antennas are plasmonic nanostructures with a strong electromagnetic field generated at the gap region of two neighboring particles owing to the coupling of the collective surface plasmon resonance. They have great potential for improving the optical properties of fluorophores. Herein, nanogap antennas are constructed using an aqueous solution-based method to overcome the defects of weak fluorescence and photobleaching associated with traditional organic dyes, and a highly sensitive nanogap antenna-based sensing strategy is presented for the detection of low-abundance nucleic acid biomarkers via a target-triggered strand displacement amplification (SDA) reaction between two DNA hairpins that are tagged to the tips of gold nanorods (Au NRs). In the presence of targets, end-to-end Au NR dimers gradually form, and the fluorophores quenched by the Au NRs exhibit a dramatic fluorescence enhancement due to the plasmon-enhanced fluorescence effect of nanogap antennas. Meanwhile, the SDA reaction results in secondary amplification of fluorescence signals. Combined with single-molecule counting, this method applied in miRNA-21 detection can achieve a low detection limit of 97.2 × 10-18 m. Moreover, accurate discrimination between different cells through miRNA-21 imaging demonstrates the potential of this method in monitoring the expression level of low-abundance nucleic acid biomarkers.
This paper references
Plasmon-Enhanced Fluorescence-Based Core–Shell Gold Nanorods as a near-IR Fluorescent Turn-On Sensor for the Highly Sensitive Detection of Pyrophosphate in Aqueous Solution
L. Wang (2015)
Thousand-fold enhancement of single-molecule fluorescence near a single gold nanorod.
Haifeng Yuan (2013)
DNA Origami Nanoantennas with over 5000-fold Fluorescence Enhancement and Single-Molecule Detection at 25 μM.
A. Puchkova (2015)
Plasmon-enhanced fluorescence spectroscopy.
Jian-Feng Li (2017)
Plasmon-Enhanced Fluorescence Biosensors: a Review
Martin Bauch (2013)
Plasmonically Engineered Nanoprobes for Biomedical Applications.
A. Kumar (2016)
Metal-enhanced fluorescence-based RNA sensing.
K. Aslan (2006)
A Ratiometric Fluorescent Bioprobe Based on Carbon Dots and Acridone Derivate for Signal Amplification Detection Exosomal microRNA.
Yaokun Xia (2018)
Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification.
T. Tian (2013)
Large single-molecule fluorescence enhancements produced by a bowtie nanoantenna
A. Kinkhabwala (2009)
Large scale solution assembly of quantum dot-gold nanorod architectures with plasmon enhanced fluorescence.
D. Nepal (2013)
Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site.
Fu Chang (2019)
Multicomponent Plasmonic Nanoparticles: From Heterostructured Nanoparticles to Colloidal Composite Nanostructures.
Minji Ha (2019)
Plasmon-enhanced fluorescence from single fluorophores end-linked to gold nanorods.
Y. Fu (2010)
Synthesis and optical properties of small Au nanorods using a seedless growth technique.
Moustafa R K Ali (2012)
Silica-Coated Plasmonic Metal Nanoparticles in Action.
Christoph Hanske (2018)
miRNAs, Cancer, and Stem Cell Division
C. Croce (2005)
DNA Origami Directed Au Nanostar Dimers for Single-Molecule Surface-Enhanced Raman Scattering.
S. Tanwar (2017)
Nanowire-Intensified Metal-Enhanced Fluorescence in Hybrid Polymer-Plasmonic Electrospun Filaments.
A. Camposeo (2018)
A comparative study of plasmonic-enhanced single-molecule fluorescence induced by gold nanoantennas and its application for illuminating telomerase.
Caixia Niu (2017)
Surface-Plasmon-Coupled Fluorescence Enhancement Based on Ordered Gold Nanorod Array Biochip for Ultrasensitive DNA Analysis.
Z. Mei (2017)
In Situ Amplification-Based Imaging of RNA in Living Cells.
Z. Qing (2019)
Self-Assembled Nanoparticle Dimer Antennas for Plasmonic-Enhanced Single-Molecule Fluorescence Detection at Micromolar Concentrations
Deep Punj (2015)
Super-resolution Localization and Defocused Fluorescence Microscopy on Resonantly Coupled Single-Molecule, Single-Nanorod Hybrids
L. Su (2016)
Directing Single-Molecule Emission with DNA Origami-Assembled Optical Antennas.
Kristina Hübner (2019)
Multiplexed Detection of Cytokines Based on Dual Bar-Code Strategy and Single-Molecule Counting.
W. Li (2016)
Distance and plasmon wavelength dependent fluorescence of molecules bound to silica-coated gold nanorods.
Nardine S. Abadeer (2014)
Biosensing with plasmonic nanosensors.
Jeffrey N. Anker (2008)
Biocompatible gold nanorods: one-step surface functionalization, highly colloidal stability, and low cytotoxicity.
Kang Liu (2015)
Target-Induced Catalytic Assembly of Y-Shaped DNA and Its Application for In Situ Imaging of MicroRNAs.
Chang Xue (2018)
DNA-Assembled Advanced Plasmonic Architectures.
N. Liu (2018)
Single-Molecule Chemistry with Surface- and Tip-Enhanced Raman Spectroscopy.
Alyssa B. Zrimsek (2017)
Preferential end-to-end assembly of gold nanorods by biotin-streptavidin connectors.
K. Caswell (2003)
Plasmon-Amplified Endogenous Fluorescence Nanospectroscopic Sensor Based on Inherent Elastic Scattering for Ultratrace Ratiometric Detection of Capsaicinoids.
Suresh Kumar Chakkarapani (2019)
Native MicroRNA Targets Trigger Self-Assembly of Nanozyme-Patterned Hollowed Nanocuboids with Optimal Interparticle Gaps for Plasmonic-Activated Cancer Detection.
J. Li (2019)
DNA-Mediated Self-Assembly of Plasmonic Antennas with a Single Quantum Dot in the Hot Spot.
F. Nicoli (2019)
Probing distance-dependent plasmon-enhanced near-infrared fluorescence using polyelectrolyte multilayers as dielectric spacers.
Naveen Gandra (2014)
Near-Infrared-Fluorescent Probes for Bioapplications Based on Silica-Coated Gold Nanobipyramids with Distance-Dependent Plasmon-Enhanced Fluorescence.
Caixia Niu (2016)
Plasmon-enhanced optical sensors: a review.
M. Li (2015)

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