Online citations, reference lists, and bibliographies.

Production Of Trehalose From Starch By Thermostable Enzymes From Sulfolobus Acidocaldarius

K. Mukai, A. Tabuchi, T. Nakada, T. Shibuya, H. Chaen, S. Fukuda, M. Kurimoto, Y. Tsujisaka
Published 1997 · Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
The optimum conditions for the production of trehalose from starch were investigated using two thermostable enzymes, maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose trehalohydrolase (MTHase), from Sulfolobus acidocaldarius ATCC 33909. The optimum pH was 5.5 and the optimum temperature was 55—57°C using isoamylase from Pseudomonas amyloderamosa as a debranching enzyme. The addition of CGTase to the reaction mixture during the saccharification process caused an increase in trehalose and a decrease in maltose and maltotriose. Isoamylase was better than pullulanase as a debranching enzyme. The yield of trehalose was independent of the type of starch used. Under optimum conditions, the yield of trehalose from corn starch at 30% concentration was more than 82%.
This paper references



This paper is referenced by
10.1007/s00792-002-0283-6
A novel thermophilic fusion enzyme for trehalose production
D. de Pascale (2002)
10.1016/J.CARBPOL.2009.10.059
Conversion of cassava starch to trehalose by Saccharomycopsis fibuligera A11 and purification of trehalose
Ying Zhang (2010)
Novel sulfolobus solfataricus trehalose hydrolase, gene of hydrolase, recombinant expression vector containing gene, and recombinant bacterium, and preparation of hydrolase
陈平平 (2013)
10.5458/JAG.56.127
Arthrobacter globiformis M6における環状マルトシルマルトースの合成および分解に関与する酵素
Tetsuya Mori (2009)
10.1002/9780470054581.EIB298
Enzymes, Starch Conversion
Sven Pedersen (2010)
Enzymes Involved in the Biosynthesis and Degradation of Cyclic Maltosyl-maltose in Arthrobacter globiformis M 6
T. Mori (2009)
Purification and Characterization of Glucosyltransferase Glucanotransferase Involved in the Production of Cyclic Tetrasaccharide in Bacillus globisporus Cll and JSBA
Tomoyuki Nishimoto (2017)
10.1128/AEM.64.11.4340-4345.1998
Purification and Characterization of a Trehalose Synthase from the Basidiomycete Grifola frondosa
K. Saito (1998)
10.1016/S0040-4039(98)02662-8
Novel synthesis of l-iduronic acid using trehalose as the disaccharidic starting material
H. Hinou (1999)
10.1002/3527600035.BPOL7013
Enzymes for Technical Applications
T. Schäfer (2002)
10.1016/B978-0-12-396523-3.00001-4
Enzymatic conversions of starch.
P. Tomasik (2012)
10.1007/s00253-009-1863-5
Gene cloning, expression, and characterization of a novel trehalose synthase from Arthrobacter aurescens
Wu Xiu-li (2009)
新型麦芽寡糖基海藻糖水解酶、酶基因、含该基因的重组表达载体及重组工程菌及酶的制备
陈平平 (2013)
10.4014/jmb.1802.02038
Saci_1816: A Trehalase that Catalyzes Trehalose Degradation in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius.
Junho Lee (2018)
10.1002/MASY.19991440122
Chemoenzymatic synthesis of biodegradable polymers containing glucobiose branches
M. Kitagawa (1999)
10.1271/bbb.60131
A Novel Glucanotransferase from a Bacillus circulans Strain That Produces a Cyclomaltopentaose Cyclized by an α-1,6-Linkage
H. Watanabe (2006)
Bifunctional recombinant fusion enzyme between maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase of thermophilic microorganism Metallosphaera hakonensis.
Ju-Seok Seo (2008)
10.1271/bbb.66.1806
Purification and Characterization of Glucosyltransferase and Glucanotransferase Involved in the Production of Cyclic Tetrasaccharide in Bacillus globisporus C11
Tomoyuki Nishimoto (2002)
10.1007/978-3-642-38954-2_329
The Family Sulfolobaceae
Sonja-Verena Albers (2014)
10.5458/JAG.51.63
α,α-トレハロースの生産技術の開発とその用途開発
倫夫 久保田 (2004)
10.4014/JMB.1101.10068
Molecular cloning of maltooligosyltrehalose trehalohydrolase gene from Nostoc flagelliforme and trehalose-related response to stresses.
S. Wu (2011)
10.1074/JBC.274.49.34868
The Cyclization Mechanism of Cyclodextrin Glycosyltransferase (CGTase) as Revealed by a γ-Cyclodextrin-CGTase Complex at 1.8-Å Resolution*
J. Uitdehaag (1999)
Trehalose accumulation from cassava starch and release by a highly thermosensitive and permeable mutant of Saccharomycopsis fibuligera
Ming-Xin ZhaoJing (2011)
10.1007/s00449-018-2039-4
High-efficiency expression of Sulfolobus acidocaldarius maltooligosyl trehalose trehalohydrolase in Escherichia coli through host strain and induction strategy optimization
Lingqia Su (2018)
10.5458/JAG.51.135
6-α-Glucosyltransferase と α-Isomaltosyltransferase による環状四糖生成
西本 友之 (2004)
10.1016/J.CARRES.2005.03.010
An enzymatically produced novel cyclic tetrasaccharide, cyclo-{→6)-α-d-Glcp-(1→4)-α-d-Glcp-(1→6)-α-d-Glcp-(1→4)-α-d-Glcp-(1→} (cyclic maltosyl-(1→6)-maltose), from starch
K. Mukai (2005)
10.1007/s10295-009-0644-6
Trehalose accumulation from corn starch by Saccharomycopsis fibuligera A11 during 2-l fermentation and trehalose purification
Zhe Chi (2009)
10.1016/S0141-0229(00)00318-5
Trehalose accumulation from soluble starch by Saccharomycopsis fibuligera sdu.
Z. Chi (2001)
10.1007/s00792-004-0393-4
Characterization of the trehalosyl dextrin-forming enzyme from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092
Tsuei-Yun Fang (2004)
Molecular cloning and characterization of trehalose biosynthesis genes from hyperthermophilic archaebacterium Metallosphaera hakonesis.
Ju-Seok Seo (2007)
10.1016/S0014-5793(00)02427-3
Enzymatic synthesis of a novel trehalose derivative, 3,3′‐diketotrehalose, and its potential application as the trehalase enzyme inhibitor
K. Sode (2001)
10.1134/S0003683819060115
SpyCatcher/SpyTag-Mediated Self-Assembly of a Supramolecular Complex for Improved Biocatalytic Production of Trehalose
X. Shen (2019)
See more
Semantic Scholar Logo Some data provided by SemanticScholar