Online citations, reference lists, and bibliographies.
← Back to Search

Nitric Oxide Inhibits Electron Transfer And Increases Superoxide Radical Production In Rat Heart Mitochondria And Submitochondrial Particles.

J. Poderoso, M. C. Carreras, C. Lisdero, N. Riobo, F. Schöpfer, A. Boveris
Published 1996 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Nitric oxide (.NO) released by S-nitrosoglutathione (GSNO) inhibited enzymatic activities of rat heart mitochondrial membranes. Cytochrome oxidase activity was inhibited to one-half at an effective .NO concentration of 0.1 microM, while succinate- and NADH-cytochrome-c reductase activities were half-maximally inhibited at 0.3 microM .NO. Submitochondrial particles treated with .NO (either from GSNO or from a pure solution) showed increased O(-)(2) and H202 production when supplemented with succinate alone, at rates that were comparable to those of control particles with added succinate and antimycin. Rat heart mitochondria treated with .NO also showed increased H2O2 production. Cytochrome spectra and decreased enzymatic activities in the presence of .NO are consistent with a multiple inhibition of mitochondrial electron transfer at cytochrome oxidase and at the ubiquinone-cytochrome b region of the respiratory chain, the latter leading to the increased O2- production. Electrochemical detection showed that the buildup of a .NO concentration from GSNO was interrupted by submitochondrial particles supplemented with succinate and antimycin and was restored by addition of superoxide dismutase. The inhibitory effect of .NO on cytochrome oxidase was also prevented under the same conditions. Apparently, mitochondrial O2- reacts with .NO to form peroxynitrate and, by removing .NO, reactivates the previously inhibited cytochrome oxidase. It is suggested that, at physiological concentrations of .NO, inhibition of electron transfer, .NO-induced O2- production, and ONOO- formation participate in the regulatory control of mitochondrial oxygen uptake.
This paper references

This paper is referenced by
The signaling pathway PI3K/Akt/Nrf2/HO-1 plays a role in the mitochondrial protection promoted by astaxanthin in the SH-SY5Y cells exposed to hydrogen peroxide
F. B. Brasil (2021)
The Isothiocyanate Sulforaphane Depends on the Nrf2/γ-GCL/GSH Axis to Prevent Mitochondrial Dysfunction in Cells Exposed to Methylglyoxal
F. B. Brasil (2021)
Role of Nitroglycerine Injection in Japanese Quail Coturnix japonica in Some Blood Parameters
M. A. Al-Bayar (2021)
Carnosic acid depends on glutathione to promote mitochondrial protection in methylglyoxal-exposed SH-SY5Y cells.
I. C. C. de Souza (2021)
The Bioactivity of Neuronal-Derived Nitric Oxide in Aging and Neurodegeneration: Switching Signaling to Degeneration.
A. Ledo (2020)
Nitric Oxide in Macrophage Immunometabolism: Hiding in Plain Sight
Erika M Palmieri (2020)
Temporal evolution of cardiac mitochondrial dysfunction in a type 1 Diabetes model. Mitochondrial complex I impairment, and H2O2 and NO productions as early subcellular events.
Ivana A. Rukavina-Mikusic (2020)
Promotion of Mitochondrial Protection by Emodin in Methylglyoxal-Treated Human Neuroblastoma SH-SY5Y Cells: Involvement of the AMPK/Nrf2/HO-1 Axis
M. R. de Oliveira (2020)
Protective Impact of Edaravone Against ZnO NPs-induced Oxidative Stress in the Human Neuroblastoma SH-SY5Y Cell Line.
Sanjiv Singh (2020)
Synthesis, docking and evaluation of in vitro anti-inflammatory activity of novel morpholine capped β-lactam derivatives.
R. Heiran (2020)
Effects of sulforaphane on brain mitochondria: mechanistic view and future directions
F. R. Jardim (2020)
Nitric Oxide Circumvents Virus-Mediated Metabolic Regulation during Human Cytomegalovirus Infection
Rebekah L. Mokry (2020)
Mitochondrial Protection and Anti-inflammatory Effects Induced by Emodin in the Human Neuroblastoma SH-SY5Y Cells Exposed to Hydrogen Peroxide: Involvement of the AMPK/Nrf2 Signaling Pathway.
M. R. de Oliveira (2020)
The Relationship Between Reactive Oxygen Species and Endothelial Cell Metabolism
Raid Alhayaza (2020)
The effect of heat stress on hematological parameters and oxidative stress among bakery workers
V. Gharibi (2020)
Hypoxic-Ischemic Encephalopathy and Mitochondrial Dysfunction: Facts, Unknowns and Challenges.
Marianela Rodríguez (2020)
Functional interactions between complex I and complex II with nNOS in regulating cardiac mitochondrial activity in sham and hypertensive rat hearts.
Yu Na Wu (2020)
Nitric Oxide
D. Thomas (2020)
Биоэнергетика миокарда в условиях гипоксии: возрастные аспекты
O. Tretyakova (2020)
A New Approach for the Prevention and Treatment of Cardiovascular Disorders. Molecular Hydrogen Significantly Reduces the Effects of Oxidative Stress
Tyler W LeBaron (2019)
Oxidative Stress and Varicocele Pathophysiology
A. Majzoub (2019)
Oxidative stress, nitric oxide and inflammation in the pathophysiology of varicocele and the effect of hydrogen sulfide as a potential treatment
Keivan Lorian (2019)
Is it possible to combine photodynamic therapy and application of dinitrosyl iron complexes in the wound treatment?
A. Solovieva (2019)
Glutathione and Nitric Oxide: Key Team Players in Use and Disuse of Skeletal Muscle
S. Baldelli (2019)
The effects of kahweol, a diterpene present in coffee, on the mitochondria of the human neuroblastoma SH-SY5Y cells exposed to hydrogen peroxide.
C. R. Fürstenau (2019)
The origins of nitric oxide and peroxynitrite research in Uruguay: 25 years of contributions to the biochemical and biomedical sciences.
R. Radi (2019)
Nitric oxide and tumor metabolic reprogramming.
L. López-Sánchez (2019)
Utilization of Microfluidics for the Preparation of Polymeric Nanoparticles for the Antioxidant Rutin - a comparison with bulk production.
Hanh T H Vu (2019)
Inhibition of the Nrf2/HO-1 Axis Suppresses the Mitochondria-Related Protection Promoted by Gastrodin in Human Neuroblastoma Cells Exposed to Paraquat
M. R. de Oliveira (2019)
The effect of nitric oxide on mitochondrial respiration.
J. Poderoso (2019)
TNFα stimulates NO release in EA.hy926 cells by activating the CaMKKβ-AMPK-eNOS pathway.
D. Dymkowska (2019)
Cardioprotective effects of physical exercise on redox biology in mice exposed to hand-rolled cornhusk cigarette smoke.
Fernanda Dal'Maso Camera (2019)
See more
Semantic Scholar Logo Some data provided by SemanticScholar