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Multianalyte Detection Using A Capillary-based Flow Immunosensor.
U. Narang, P. R. Gauger, A. Kusterbeck, F. Ligler
Published 1998 · Chemistry, Medicine
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A highly sensitive, dual-analyte detection system using capillary-based immunosensors has been designed for explosive detection. This model system consists of two capillaries, one coated with antibodies specific for 2,4,6-trinitrotoluene (TNT) and the other specific for hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) combined into a single device. The fused silica capillaries are prepared by coating anti-TNT and anti-RDX antibodies onto the silanized inner walls using a hetero-bifunctional crosslinker. After immobilization, the antibodies are saturated with a suitable fluorophorelabeled antigen. A "T" connector is used to continuously flow the buffer solution through the individual capillaries. To perform the assay, an aliquot of TNT or RDX or a mixture of the two analytes is injected into the continuous flow stream. In each capillary, the target analyte displaces the fluorophore-labeled antigen from the binding pocket of the antibody. The labeled antigen displaced from either capillary is detected downstream using two portable spectrofluorometers. The limits of detection for TNT and RDX in the multi-analyte formate are 44 fmol (100 microliters of 0.1 ng/ml TNT solution) and 224 fmol (100 microliters of 0.5 ng/ml RDX solution), respectively. The entire assay for both analytes can be performed in less than 3 min.
This paper references
Multi-analyte immunoassays application to environmental analysis
A. Brecht (1995)
Simultaneous determination of total IgE and allergen-specific IgE in serum by the MAST chemiluminescent assay system.
C. R. Brown (1985)
Kinetics of antibody binding at solid-liquid interfaces in flow.
G. Wemhoff (1992)
Simultaneous determination of follicle stimulating hormone and luteinising hormone using a multianalyte immunosensor
D. J. Pritchard (1995)
Sequential microenzymatic assay of cholesterol, triglycerides, and phospholipids in a single aliquot.
M. Nanjee (1996)
Simultaneous radioassay of serum vitamin B12 and folic acid.
S. Gutcho (1977)
Simultaneous immunoassay using electrochemical detection of metal ion labels.
F. J. Hayes (1994)
A biosensor array based on polyaniline.
H. Sangodkar (1996)
Novel Optical Measurement Approach for the Quantitation of Liposome Immunomigration Assays
S. Reeves (1995)
A displacement flow immunosensor for explosive detection using microcapillaries
U. Narang (1997)
Simultaneous enzyme immunoassay of two thyroid hormones.
C. Blake (1982)
Capillary-Based Displacement Flow Immunosensor
U. Narang (1997)
Evaluation of simultaneous measurement of lutropin and follitropin with the SimulTROPIN radioimmunoassay kit.
F. Wians (1986)
Simultaneous measurement of total and IgA-conjugated alpha 1-microglobulin by a combined immunoenzyme/immunoradiometric assay technique.
D. DeMars (1989)
Double-label time-resolved immunofluorometry of lutropin and follitropin in serum.
I. Hemmilä (1987)
A novel coloured latex test for the detection and identification of more than one antigen.
S. Hadfield (1987)
Dual analyte assay based on particle types of different size measured by flow cytometry.
J. Frengen (1995)
Multianalyte immunoassay based on spatially distinct fluorescent areas quantified by laser-excited solid-phase time-resolved fluorometry.
S. Kakabakos (1992)
Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces.
S. Bhatia (1989)
SimulTRAC simultaneous radioimmunoassay of thyrotropin and free thyroxin evaluated.
S. Gow (1986)
Simultaneous immunoassay using piezoelectric immunosensor array and robust method
X. Chu (1996)
Developing multianalyte assays.
R. Ekins (1994)
Use of the USDT flow immunosensor for quantitation of benzoylecgonine in urine.
H. Yu (1996)
Dual-label time-resolved fluoroimmunoassay for simultaneous detection of myoglobin and carbonic anhydrase III in serum.
J. Vuori (1991)
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A. Crockett (1999)
A duplexed microsphere-based fluorescent immunoassay.
F. Szurdoki (2001)
Flow immunoassay for detection of human chorionic gonadotrophin using a cation exchange resin packed capillary column
N. Nakamura (2001)
Simultaneous detection of analytes based on genetically engineered whole cell sensing systems
S. Shrestha (2001)
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S. Saini (2010)
Sequential analysis of multiple analytes using a surface plasmon resonance (SPR) biosensor.
J. W. Chung (2006)
Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume.
S. Padmanabhan (2010)
Luminescence-based methods for sensing and detection of explosives
Melissa S Meaney (2008)
Multi-analyte explosive detection using a fiber optic biosensor
I. Bakaltcheva (1999)
Flow-through immunosensors using antibody-immobilized polymer monoliths.
J. Liu (2010)
A High Aspect Ratio Bifurcated 128-Microchannel Microfluidic Device for Environmental Monitoring of Explosives
P. Charles (2018)
A quantitative assessment of chemical techniques for detecting traces of explosives at counter-terrorist portals.
Mani Nambayah (2004)
TNT stilbene derivatives as SERRS active species.
C. McHugh (2007)
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Michael Wiederoder (2016)
Array biosensor: recent developments
J. Golden (1999)
Role of genetic and expression profiling in pharmacogenomics: the changing face of patient management.
V. Pagliarulo (2002)
Emerging Technologies for Mycotoxin Detection
C. Maragos (2004)
Chapter 1 – INTERFACIAL AND MATERIALS ASPECTS OF THE IMMOBILIZATION OF BIOMOLECULES ONTO SOLID SURFACES
W. Albers (2001)
Detection of 2,4,6-trinitrotoluene in seawater using a reversed-displacement immunosensor.
Tiffanee M Green (2002)
Chapter 5 – FLOW IMMUNOSENSORS
A. Kusterbeck (2008)
Biosensors in Forensic Analysis
P. Yáñez-Sedeño (2016)
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L. Knop (2019)
Trace level detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by microimmunosensor.
P. Charles (1999)
Multi-analyte capillary immunosensor for the determination of hormones in human serum samples.
P. Petrou (2002)
Multiplexed immunoassays in food analysis
Chien-Sheng Chen (2008)
Optical detection enhancement in porous volumetric microfluidic capture elements using refractive index matching fluids.
M. Wiederoder (2015)
Continuous-flow fluoro-immunosensor for paclitaxel measurement.
S. H. Sheikh (2001)
A review of biosensors and biologically-inspired systems for explosives detection.
R. Smith (2008)
Development of a fluorescence immunoassay for measurement of paclitaxel in human plasma.
S. H. Sheikh (2000)
Advances in Flow Displacement Immunoassay Design
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