Online citations, reference lists, and bibliographies.
← Back to Search

Contribution Of The Carboxy-terminal Domain Of Lipoprotein Lipase To Interaction With Heparin And Lipoproteins.

A. Lõokene, M. S. Nielsen, J. Gliemann, G. Olivecrona
Published 2000 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The C-terminal domain of lipoprotein lipase (LPL) is involved in several important interactions. To assess its contribution to the binding ability of full-length LPL we have determined kinetic constants using biosensor technique. The affinity of the C-terminal domain for heparin was about 500-fold lower than that of full-length LPL (K(d) = 1.3 microM compared to 3.1 nM). Replacement of Lys403, Arg405 and Lys407 by Ala abolished the heparin affinity, whereas replacement of Arg420 and Lys422 had little effect. The C-terminal domain increased binding of chylomicrons and VLDL to immobilized heparin relatively well, but was less than 10% efficient in binding of LDL compared to full-length LPL. Deletion of residues 390-393 (WSDW) did not change the affinity to heparin and only slightly decreased the affinity to lipoproteins. We conclude that the C-terminal folding domain contributes only moderately to the heparin affinity of full-length LPL, whereas the domain appears important for tethering triglyceride-rich lipoproteins to heparin-bound LPL.
This paper references
10.1074/jbc.272.9.5821
Segments in the C-terminal Folding Domain of Lipoprotein Lipase Important for Binding to the Low Density Lipoprotein Receptor-related Protein and to Heparan Sulfate Proteoglycans*
M. Nielsen (1997)
Binding of lipoprotein lipase to heparin. Identification of five critical residues in two distinct segments of the amino-terminal domain.
A. Hata (1993)
10.1006/BBRC.1998.9596
Mild oxidation of lipoproteins increases their affinity for surfaces covered by heparan sulfate and lipoprotein lipase.
E. Makoveichuk (1998)
The alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein binds lipoprotein lipase and beta-migrating very low density lipoprotein associated with the lipase.
A. Nykjaer (1993)
10.1016/0076-6879(91)97160-Z
Phospholipase activity of milk lipoprotein lipase.
G. Bengtsson-Olivecrona (1991)
A carboxyl-terminal fragment of lipoprotein lipase binds to the low density lipoprotein receptor-related protein and inhibits lipase-mediated uptake of lipoprotein in cells.
A. Nykjaer (1994)
10.1074/jbc.272.2.766
Mutation of Tryptophan Residues in Lipoprotein Lipase
A. Lõokene (1997)
10.1111/J.1432-1033.1993.TB17747.X
Chymotryptic cleavage of lipoprotein lipase. Identification of cleavage sites and functional studies of the truncated molecule.
A. Lõokene (1993)
Mutagenesis in four candidate heparin binding regions (residues 279-282, 291-304, 390-393, and 439-448) and identification of residues affecting heparin binding of human lipoprotein lipase.
Y. Ma (1994)
10.1021/BI991512X
Characterization of heparin binding of human extracellular superoxide dismutase.
A. Lõokene (2000)
10.1111/J.1432-1033.1991.TB15913.X
Comparison of the action of lipoprotein lipase on triacylglycerols and phospholipids when presented in mixed liposomes or in emulsion droplets.
C. Rojas (1991)
10.1021/BI00361A023
Kinetics of interaction of C1 inhibitor with complement C1s.
M. Lennick (1986)
10.1097/00041433-199404000-00008
Structure, function and role of lipoprotein lipase in lipoprotein metabolism.
S. Santamarina-Fojo (1994)
Lipoproteins in Health and Diseases
J. Betteridge (1999)
10.1017/S0033583500002031
The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides.
G. Manning (1978)
Remnant lipoprotein metabolism: key pathways involving cell-surface heparan sulfate proteoglycans and apolipoprotein E.
R. Mahley (1999)
Lipoprotein lipase. Molecular model based on the pancreatic lipase x-ray structure: consequences for heparin binding and catalysis.
H. van Tilbeurgh (1994)
10.5694/j.1326-5377.1973.tb128705.x
Blood Constituents. (Book Reviews: Blood Lipids and Lipoproteins. Quantitation, Composition, and Metabolism)
G. Nelson (1972)
10.1021/BI962699K
Interaction of lipoproteins with heparan sulfate proteoglycans and with lipoprotein lipase. Studies by surface plasmon resonance technique.
A. Lõokene (1997)
10.1042/BJ3140563
Lipoprotein lipase stimulates the binding and uptake of moderately oxidized low-density lipoprotein by J774 macrophages.
W. L. Hendriks (1996)
Chimeras of hepatic lipase and lipoprotein lipase. Domain localization of enzyme-specific properties.
R. Davis (1992)
Cellular differences in lipoprotein lipase-mediated uptake of low density lipoproteins.
J. Obunike (1994)
10.1016/0014-5793(91)80997-H
Lipoprotein lipases and vitellogenins in relation to the known three‐dimensional structure of pancreatic lipase
B. Persson (1991)
10.1021/BI960008E
Interaction of lipoprotein lipase with heparin fragments and with heparan sulfate: stoichiometry, stabilization, and kinetics.
A. Lõokene (1996)
Lipoprotein lipase enhances removal of chylomicrons and chylomicron remnants by the perfused rat liver.
N. Skottová (1995)
Lipoprotein lipase domain function.
H. Wong (1994)
10.1016/b978-0-12-186060-8.x5000-1
Heparin-Binding Proteins
H. Conrad (1997)
Identification of a heparin-binding domain in the distal carboxyl-terminal region of lipoprotein lipase by site-directed mutagenesis.
R. A. Sendak (1998)
10.1042/BJ2720605
Interaction of heparin with fibronectin and isolated fibronectin domains.
K. Ingham (1990)
The carboxyl-terminal domain of lipoprotein lipase binds to the low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor (LRP) and mediates binding of normal very low density lipoproteins to LRP.
S. Williams (1994)



This paper is referenced by
10.1016/j.drudis.2016.10.007
Emerging strategies of targeting lipoprotein lipase for metabolic and cardiovascular diseases.
W. Geldenhuys (2017)
Angiopoietin-like protein 4 : an unfolding chaperone regulating lipoprotein lipase activity
V. Sukonina (2007)
10.1074/jbc.M202893200
Site-directed Mutagenesis of the Basic N-terminal Cluster of Pancreatic Bile Salt-dependent Lipase
Emeline Aubert (2002)
10.1074/jbc.M604702200
Isolation and Characterization of Low Sulfated Heparan Sulfate Sequences with Affinity for Lipoprotein Lipase*
D. Spillmann (2006)
10.1074/jbc.M114.634626
Evidence for Two Distinct Binding Sites for Lipoprotein Lipase on Glycosylphosphatidylinositol-anchored High Density Lipoprotein-binding Protein 1 (GPIHBP1)*
Mart Reimund (2015)
10.1021/acs.biochem.6b00945
Biochemical Analysis of the Lipoprotein Lipase Truncation Variant, LPLS447X, Reveals Increased Lipoprotein Uptake.
Cassandra K. Hayne (2017)
10.1016/J.MOLCATB.2006.02.015
Biosensors for the evaluation of lipase activity
N. F. Starodub (2006)
10.1016/j.atherosclerosis.2011.07.019
Apolipoprotein A-V; a potent triglyceride reducer.
S. K. Nilsson (2011)
10.1002/jcla.22414
Rare LPL gene missense mutation in an infant with hypertriglyceridemia
Y. Qin (2018)
10.33549/PHYSIOLRES.932854
Endogenous LPS alters liver GH/IGF system gene expression and plasma lipoprotein lipase in goats.
Z. Xie (2015)
10.1007/s00109-002-0384-9
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
10.1194/jlr.M088807
On the mechanism of angiopoietin-like protein 8 for control of lipoprotein lipase activity
O. Kovrov (2019)
10.1089/met.2009.0015
Lipid profiles and associated gene polymorphisms in young Asian Indian patients with acute myocardial infarction and the metabolic syndrome.
N. Ranjith (2009)
10.1194/jlr.M500552-JLR200
Substrate specificity of lipoprotein lipase and endothelial lipase: studies of lid chimeras Published, JLR Papers in Press, May 10, 2006.
N. Griffon (2006)
10.14288/1.0091300
Chimeras of lipoprotein lipase and hepatic lipase : localization of the apolipoprotein C-II activation site of lipoprotein lipase
T. McIlhargey (2003)
10.1016/j.atherosclerosis.2009.12.027
The metabolism of triglyceride-rich lipoproteins revisited: new players, new insight.
G. Dallinga-Thie (2010)
Identification of the nucleotide sequence of the lipoprotein lipase gene as well as its role in the development of hyperlipidemia and pancreatitis in the Miniature Schnauzer
A. Stolle (2005)
10.1172/JCI11774
Heparin-binding defective lipoprotein lipase is unstable and causes abnormalities in lipid delivery to tissues.
E. Lutz (2001)
Preparation of Heparin Surface for Quantification of Fibroblast Growth Factor-2 (FGF-2) Binding Using Surface Plasmon Resonance (SPR).
D. R. Kirtland (2005)
10.1074/jbc.M802579200
The Acidic Domain of GPIHBP1 Is Important for the Binding of Lipoprotein Lipase and Chylomicrons*
P. Gin (2008)
10.1007/978-3-642-00300-4_13
The Ins and Outs of Adipose Tissue
T. Olivecrona (2009)
10.1016/S0378-1119(02)00680-7
Lipoprotein lipase from rainbow trout differs in several respects from the enzyme in mammals.
Anna Lindberg (2002)
10.2108/zsj.30.224
Molecular Cloning and Transcript Expression of Genes Encoding Two Types of Lipoprotein Lipase in the Ovary of Cutthroat Trout, Oncorhynchus clarki
Yong-Woon Ryu (2013)
10.1002/psc.2413
Kinetic and functional characterisation of the heparin‐binding peptides from human transglutaminase 2
K. Teesalu (2012)
10.1007/s00253-005-0161-0
A unique polypeptide from the C-terminus of the exocellular esterase of Acinetobacter venetianus RAG-1 modulates the emulsifying activity of the polymeric bioemulsifier apoemulsan
H. Bach (2006)
10.1101/gad.209296.112
Biochemistry and pathophysiology of intravascular and intracellular lipolysis.
S. Young (2013)
10.1007/978-1-4615-1517-3
Genetics of Dyslipidemia
P. Benlian (2001)
10.1194/jlr.M072520
Mobility of “HSPG-bound” LPL explains how LPL is able to reach GPIHBP1 on capillaries[S]
C. Allan (2016)
Novel factors affecting clearance of triacylglycerol-rich lipoproteins from blood
S. K. Nilsson (2010)
Characterization of the lipolytic activity of endothelial lipase.
M. McCoy (2002)
10.1161/HQ0102.101551
Lipoprotein Lipase in the Arterial Wall: Linking LDL to the Arterial Extracellular Matrix and Much More
M. Pentikäinen (2002)
10.1016/j.bbrc.2014.06.114
Identification of a small molecule that stabilizes lipoprotein lipase in vitro and lowers triglycerides in vivo.
M. Larsson (2014)
See more
Semantic Scholar Logo Some data provided by SemanticScholar