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Silkworm Hemolymph Inhibits Baculovirus-induced Insect Cell Apoptosis.
W. J. Rhee, T. Park
Published 2000 · Biology, Medicine
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The effect of silkworm hemolymph on baculovirus-induced insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity; however, its addition after the infection was less effective. This can be explained by the higher transfer rate of silkworm hemolymph which is caused by endocytosis during the virus internalization step. The delayed cell death due to silkworm hemolymph was not caused by an inhibition of the virus attachment and internalization steps. The apoptosis was analyzed using DNA fragmentation and TUNEL assays, and the resulting data confirm that silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
This paper references
The chemistry of insect hemolymph; organic components of the hemolymph of the silkworm, Bombyx mori, and two other species.
G. R. Wyatt (1956)
Kinetic Effect of Silkworm Hemolymph on the Delayed Host Cell Death in an Insect Cell‐Baculovirus System
Won Jong Rhee (1999)
Experiments in molecular genetics
J. Miller (1972)
Role of early and late replication events in induction of apoptosis by baculoviruses.
D. J. Lacount (1997)
Kinetic characterization of baculovirus‐induced cell death in insect cell cultures
S. C. Wu (1993)
Induction of bcl-2 expression by epstein-barr virus latent membrane protein 1 protects infected B cells from programmed cell death
S. Henderson (1991)
Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death
D. Hockenbery (1990)
Establishment of Four Strains of Cells from Insect Tissues Grown in vitro
T. Grace (1962)
A conserved family of cellular genes related to the baculovirus iap gene and encoding apoptosis inhibitors.
C. Duckett (1996)
An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif.
N. Crook (1993)
On the Interpretation of Multi-Hit Survival Curves.
K. Atwood (1949)
Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture
S. Ha (1996)
Apoptosis by a cytosolic extract from Fas‐activated cells.
M. Enari (1995)
An apoptosis-inhibiting gene from a nuclear polyhedrosis virus encoding a polypeptide with Cys/His sequence motifs.
M. Birnbaum (1994)
Identification and characterization of the p35 gene of Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis.
S. G. Kamita (1993)
Apoptosis reduces both the in vitro replication and the in vivo infectivity of a baculovirus.
R. Clem (1993)
Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes
Peter Listen (1996)
Prevention of apoptosis by a baculovirus gene during infection of insect cells.
R. Clem (1991)
CULTURE IN VITRO OF TISSUE FROM THE SILKWORM, BOMBYX MORI L
S. Wyatt (1956)
Baculovirus expression systems and biopesticides.
M. Shuler (1994)
Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6
E. Yonish-Rouach (1991)
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L. M. Martins (2011)
Inhibition of HeLa Cell Apoptosis by Storage‐Protein 2
W. J. Rhee (2007)
Enhancement of sialyltransferase-catalyzed transfer of sialic acid onto glycoprotein oligosaccharides using silkworm hemolymph and its 30K protein
S. Choi (2006)
Enhancement of L-Asparaginase Activity in L5178Y Mouse Lymphoma Cells by Conjugating 30Kc19 Protein and L-Asparaginase by Cleavable Linker
S. J. Yang (2016)
Anti-apoptotic mechanism of silkworm hemolymph in HeLa cell apoptosis
W. J. Rhee (2013)
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Wojska Polskiego (2016)
Identification and characterization of a novel cell-penetrating peptide of 30Kc19 protein derived from Bombyx mori
Hee Ho Park (2014)
Enhancement of Sf‐9 Cell Growth and Longevity through Supplementation of Culture Medium with Hemolymph
L. Maranga (2003)
Purification and Characterization of an Anti‐Apoptotic Protein Isolated from Lonomia obliqua Hemolymph
Alvaro P. B. Souza (2005)
Inhibition of apoptosis by recombinant 30K protein originating from silkworm hemolymph.
E. H. Kim (2003)
Dimerization of 30Kc19 protein in the presence of amphiphilic moiety and importance of Cys-57 during cell penetration
Hee Ho Park (2014)
Effect of silkworm hemolymph on N‐linked glycosylation in two Trichoplusia ni insect cell lines
C. Joosten (2003)
α-Galactosidase delivery using 30Kc19-human serum albumin nanoparticles for effective treatment of Fabry disease
Hong Jai Lee (2016)
Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells.
W. J. Rhee (2002)
Notice of RetractionApoptosis Induced by Actinomycin D in Spodoptera litura Cells and the Effect of Two Insect Hemolymph on Apoptosis
Rui Zhang (2011)
Identification and Characterization of Cell-penetrating Peptide from 30Kc19 Protein and Its Application to Protein and Gene Delivery
Enhancing effect of a protein from Lonomia obliqua hemolymph on recombinant protein production
R. Mendonça (2008)
Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages
Mi-Ra Chang (2013)
CHO 세포에서 누에 혈림프 유래 Storage-protein 2의 세포응집 및 세포사멸 억제 효과
Inhibition of apoptosis in HeLa cell by silkworm storage protein 1, SP1
J. Lee (2015)
Protein‐stabilizing and cell‐penetrating properties of α‐helix domain of 30Kc19 protein
Jina Ryu (2016)
Enhanced production of recombinant protein inEscherichia coli using silkworm hemolymph
J. Kim (2005)
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You Ree Nam (2018)
Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles.
Hong Jai Lee (2014)
Improved replication of the baculovirus Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) in vitro using proteins from Lonomia obliqua hemolymph
Á. P. B. Sousa (2014)
Purification of recombinant 30K protein produced in Escherichia coli and its anti-apoptotic effect in mammalian and insect cell systems
H. J. Park (2003)
Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori
Jian-qing Chen (2015)
Lysine acetylation stabilizes SP2 protein in the silkworm Bombyx mori.
Y. Zhou (2016)
Two Crystal Structures of Bombyx mori Lipoprotein 3 - Structural Characterization of a New 30-kDa Lipoprotein Family Member
A. Pietrzyk (2013)
Insect cell culture for industrial production of recombinant proteins
L. Ikonomou (2003)
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Jina Ryu (2015)
The genomic underpinnings of apoptosis in the silkworm, Bombyx mori
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