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Protective Effect Of N-acetylcysteine In Tumor Necrosis Factor-alpha-induced Apoptosis In U937 Cells: The Role Of Mitochondria.

A. Cossarizza, C. Franceschi, D. Monti, S. Salvioli, E. Bellesia, R. Rivabene, L. Biondo, G. Rainaldi, A. Tinari, W. Malorni
Published 1995 · Biology, Medicine

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The existence of two different pathways for cell death has been postulated. In addition to the passive and traumatic process leading to necrosis, an active program characterized by organelle integrity and called apoptosis has been described. A positive correlation between the apoptotic cell death process and oxidative imbalance has been demonstrated. In fact, the antioxidant N-acetylcysteine (NAC) seems to be capable of impairing the apoptotic program, replenishing intracellular reduced glutathione content in cells exposed to tumor necrosis factor-alpha (TNF) as apoptotic inducer. Moreover, protein synthesis inhibitors such as cycloheximide (CHX) can facilitate apoptotic triggering by TNF, and mitochondrial function was suggested to be essential in the TNF-mediated apoptotic process. With this in mind, a specific analysis using the JC-1 probe, a fluorescent dye which is capable of indicating mitochondrial membrane potential (delta psi m) changes, was carried out. Our results show that TNF exposure is capable of altering the mitochondria and that NAC protection from CHX + TNF-induced apoptosis could be due to a direct effect of the drug on mitochondrial integrity and function.



This paper is referenced by
10.1074/JBC.M100571200
Abrin Triggers Cell Death by Inactivating a Thiol-specific Antioxidant Protein*
S. Shih (2001)
10.1016/S0361-9230(02)00830-4
Protection of cultured oligodendrocytes against tumor necrosis factor-α by the antioxidants coenzyme Q10 and N-acetyl cysteine
W. Cammer (2002)
10.1177/153537020322801113
Different Sensitivity to Apoptosis in Cells of Monocytic or Lymphocytic Origin Chronically Infected with Human Immunodeficiency Virus Type-1
M. Pinti (2003)
10.4081/jbr.2016.5950
Differential co-expression network analysis for identification of functional modules in HIV infection and tubercolosis-HIV co-infection
Mohit Jha (2016)
10.5045/br.2019.54.4.253
Potential biomarkers and antagonists for fluoranthene-induced cellular toxicity of bone marrow derived mesenchymal stem cells
Md Moinul Hoque (2019)
10.1002/(SICI)1098-2744(199707)19:2<101::AID-MC5>3.0.CO;2-I
Suppression of nitric oxide–induced apoptosis by N‐acetyl‐l‐cysteine through modulation of glutathione, bcl‐2, and bax protein levels
Y. Ho (1997)
10.1007/978-3-0348-9088-5_25
Stress proteins in inflammation.
B. Polla (1998)
10.4049/jimmunol.171.10.5188
T Cell Activation-Induced Mitochondrial Hyperpolarization Is Mediated by Ca2+- and Redox-Dependent Production of Nitric Oxide 1
G. Nagy (2003)
10.1016/S0014-5793(02)02972-1
Mitochondria hyperpolarization is an early event in oxidized low‐density lipoprotein‐induced apoptosis in Caco‐2 intestinal cells
C. Giovannini (2002)
10.1016/S0002-9440(10)62577-1
Type I interferon gene transfer sensitizes melanoma cells to apoptosis via a target activity on mitochondrial function.
P. Matarrese (2002)
Early changes in intramitochondrial cardiolipin distribution during apoptosis.
M. G. Garcia Fernandez (2002)
10.1016/J.TAAP.2004.02.011
Signal transduction of p53-independent apoptotic pathway induced by hexavalent chromium in U937 cells.
Y. Hayashi (2004)
10.1016/j.freeradbiomed.2007.11.017
Oxidation of mitochondrial peroxiredoxin 3 during the initiation of receptor-mediated apoptosis.
Andrew G. Cox (2008)
10.1016/S0014-5793(00)01547-7
Galectin‐3 overexpression protects from cell damage and death by influencing mitochondrial homeostasis
P. Matarrese (2000)
10.1146/ANNUREV.PHYSIOL.60.1.619
The mitochondrial death/life regulator in apoptosis and necrosis.
G. Kroemer (1998)
10.1038/sj.cdd.4400501
N-acetylcysteine blocks apoptosis induced by N-α-tosyl-L-phenylalanine chloromethyl ketone in transformed T-cells
V. Heussler (1999)
10.1006/BBRC.2000.3715
C60 carboxyfullerene exerts a protective activity against oxidative stress-induced apoptosis in human peripheral blood mononuclear cells.
D. Monti (2000)
10.1016/S0887-2333(02)00028-0
Epithelial cells and expression of the phagocytic marker CD68: scavenging of apoptotic bodies following Rho activation.
S. Travaglione (2002)
10.1074/jbc.M607614200
Clostridium difficile Toxin B Causes Apoptosis in Epithelial Cells by Thrilling Mitochondria
P. Matarrese (2007)
10.1016/J.BBAMCR.2007.06.003
Arsenic trioxide sensitizes promonocytic leukemia cells to TNFalpha-induced apoptosis via p38-MAPK-regulated activation of both receptor-mediated and mitochondrial pathways.
D. Amrán (2007)
10.1016/S0167-4889(97)00093-1
Reduced glutathione prevents nitric oxide-induced apoptosis in vascular smooth muscle cells.
Z. Zhao (1997)
10.1007/978-1-4615-5889-7_9
Immunosenescence: Paradoxes and New Perspectives Emerging from the Study of Healthy Centenarians
C. Franceschi (1996)
10.1007/978-3-662-22516-5_3
Oxidative Stress in Neuronal Death and Apoptosis
I. Ceballos-Picot (1997)
10.1006/EXCR.1997.3570
Mitochondrial membrane potential changes in osteoblasts treated with parathyroid hormone and estradiol.
M. B. Troyan (1997)
10.1210/JCEM.83.7.4949
N-acetyl-L-cysteine inhibits apoptosis in human male germ cells in vitro.
K. Erkkilä (1998)
10.1038/sj.cdd.4400508
Manganese induces apoptosis of human B cells: caspase-dependent cell death blocked by Bcl-2
N. Schrantz (1999)
10.1089/NEU.1999.16.1067
Effect of N-acetylcysteine on mitochondrial function following traumatic brain injury in rats.
Y. Xiong (1999)
10.1038/sj.cdd.4400616
The HIV-1 vpr protein induces anoikis-resistance by modulating cell adhesion process and microfilament system assembly
P. Matarrese (2000)
10.1086/338564
Mitochondrial functionality and mitochondrial DNA content in lymphocytes of vertically infected human immunodeficiency virus-positive children with highly active antiretroviral therapy-related lipodystrophy.
A. Cossarizza (2002)
10.1093/JN/133.8.2505
Magnesium deficiency induces apoptosis in primary cultures of rat hepatocytes.
H. Martin (2003)
10.1080/02772240701391538
Studies on mercuric sulfide, a component of cinnabar, a Chinese herbal medicine, on celluar functions in mouse lung and fibroblasts
C. C. Yen (2008)
10.1016/j.neuint.2009.01.022
Antioxidant potential of Minocycline in Japanese Encephalitis Virus infection in murine neuroblastoma cells: Correlation with membrane fluidity and cell death
M. Mishra (2009)
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