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A Composite CMV-IE Enhancer/beta-actin Promoter Is Ubiquitously Expressed In Mouse Cutaneous Epithelium.

J. Sawicki, R. Morris, B. Monks, K. Sakai, J. Miyazaki
Published 1998 · Biology, Medicine

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In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable to express a transgene in all the cells of the cutaneous epithelium. Since no ubiquitously expressed promoter sequences had previously been identified, we used lacZ reporter transgenes to test two enhancer/promoter sequences for ubiquitous expression in the skin of adult transgenic mice. We find that a CMV enhancer/CMV promoter is not active in most cell types in the skin, whereas a CMV enhancer/modified beta-actin promoter sequence is active in the suprabasal and basal cells of the epidermis as well as in the epithelial cells of the hair follicles, sebaceous glands, and the dermal papillae.
This paper references
10.1093/NAR/19.22.6205
The variability in activity of the universally expressed human cytomegalovirus immediate early gene 1 enhancer/promoter in transgenic mice.
P. Furth (1991)
10.1128/MCB.10.8.4406
The cytomegalovirus enhancer: a pan-active control element in transgenic mice.
E. Schmidt (1990)
10.1073/PNAS.94.26.14559
Spatio-temporally controlled site-specific somatic mutagenesis in the mouse.
J. Brocard (1997)
10.1016/0378-1119(86)90137-X
Powerful and versatile enhancer-promoter unit for mammalian expression vectors.
M. K. Foecking (1986)
10.2144/98255RR01
Cell-specific ecdysone-inducible expression of FLP recombinase in mammalian cells.
J. Sawicki (1998)
10.1016/B978-0-12-203455-8.50013-3
8 – Transgenic Mouse Models for the Study of the Skin
C. Cavard (1993)
10.1016/S0092-8674(85)80025-8
A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus
M. Boshart (1985)
10.1016/0378-1119(91)90434-d
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
H. Niwa (1991)
10.1046/J.1440-169X.1995.T01-2-00012.X
Green fluorescent protein as a marker in transgenic mice
M. Ikawa (1995)
10.2142/BIOPHYS.17.240
Molecular Biology of The Skin
S. Arase (1977)
10.1016/0168-9525(93)90104-P
Site-specific recombinases: tools for genome engineering.
N. J. Kilby (1993)
10.1128/JVI.64.5.2388-2391.1990
Phytohemagglutinin-induced activity of cyclic AMP (cAMP) response elements from cytomegalovirus is reduced by cyclosporine and synergistically enhanced by cAMP.
H. H. Niller (1990)
10.1016/0378-1119(91)90434-D
Efficient selection for high-expression transfectants with a novel eukaryotic vector
Niwa Hitoshi (1991)



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S. Choi (2012)
10.1038/12186
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N. Gaiano (1999)
10.1016/J.MICINF.2006.01.010
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H. Yanai (2006)
10.1038/cddis.2014.432
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10.1038/SJ.MT.6300332
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10.1002/jgm.742
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10.1002/dvdy.10104
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S. Wood (2017)
10.2144/01311RR03
Analysis of ubiquitination in vivo using a transgenic mouse model.
M. Tsirigotis (2001)
10.1073/pnas.1418401112
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10.1074/jbc.M410471200
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10.1002/stem.45
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10.3390/ijms21124197
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T. M. Buck (2020)
10.1038/gt.2009.62
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E. González-González (2009)
10.1007/978-1-61779-034-8_9
Use of (Gyro) Gy and spermine synthase transgenic mice to study functions of spermine.
X. Wang (2011)
10.1016/j.vaccine.2008.09.048
In vitro search for alternative promoters to the human immediate early cytomegalovirus (IE-cMV) to express the G gene of viral haemorrhagic septicemia virus (VHSV) in fish epithelial cells.
S. Ruiz (2008)
10.1016/S0378-1119(01)00550-9
Optimization of transcriptional regulatory elements for constructing plasmid vectors.
Z. Xu (2001)
10.1089/10445490050199045
Predominant transgene expression in exocrine pancreas directed by the CMV promoter.
Y. Zhan (2000)
10.1016/j.pep.2017.09.008
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D. Wang (2018)
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L. Cook (2011)
10.1089/HUM.2005.16.649
Adeno-associated virus-vectored gene therapy for retinal disease.
A. Dinculescu (2005)
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