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Determination Of Vitamin E In Aquatic Organisms By High-performance Liquid Chromatography With Fluorescence Detection.

J. Huo, H. Nelis, P. Lavens, P. Sorgeloos, A. D. De leenheer
Published 1996 · Chemistry, Medicine

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A liquid chromatographic (HPLC) method has been developed for the quantitative determination of different forms of vitamin E (alpha-, gamma-, and delta-tocopherol) in aquatic organisms. The assay consists of a simple extraction with methanol containing butylhydroxytoluene (BHT) as an antioxidant, followed by reversed-phase chromatography with fluorescence detection. The efficiency of the extraction method was equivalent or superior to that of more complex approaches for the isolation of tocopherols. Linearity has been achieved over the range of 0.02 to 3 micrograms/ml for alpha-tocopherol and within-run and between-run coefficients of variation at three levels were 0.7-2.9 and 1.2-3.7%, respectively. The recovery at three concentrations ranged from 73.8 to 96.6% and the minimal quantity that could be detected was 0.6 ng. Comparable figures were obtained for gamma- and delta-tocopherol. This method has been routinely applied to determine vitamin E in Artemia, rotifers, turbot and sea bass larvae, and shrimp postlarvae.



This paper is referenced by
10.1016/J.ACA.2005.10.085
Near infrared spectroscopy (NIRS) reflectance technology for determination of tocopherols in animal feeds
I. González-Martin (2006)
10.1042/CS20080224
Systemic hypoxia enhances bactericidal activities of human polymorphonuclear leuocytes.
Jong-Shyan Wang (2009)
10.1201/9781315275871
Dynamics of Chromatography: Principles and Theory
J. Giddings (2002)
10.1093/CHROMSCI/46.4.345
Simultaneous determination of five fat-soluble vitamins in feed by high-performance liquid chromatography following solid-phase extraction.
Xiuping Xue (2008)
10.1016/S0301-4770(08)60314-0
Chapter 16 - Vitamins
A. Rizzolo (1998)
The nutrition and feeding of a native Thai species, the marble goby (Oxyeleotris marmoratus), involving on-farm and experimental studies
Jatuporn Bundit (2008)
10.1006/ABIO.1999.4019
Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E.
P. Prieto (1999)
Effect of Refrigerated Storage on Fillet Lipid Quality of Rainbow Trout (Oncorhynchus mykiss W.) Fed a Diet Containing Different Levels of DL a-Tocopherol Acetate
M. Yıldız (2006)
10.1080/10408398.2012.694920
Tocopherols in Seafood and Aquaculture Products
Cláudia Afonso (2016)
10.1002/(SICI)1099-1565(199911/12)10:6<307::AID-PCA477>3.0.CO;2-L
A rapid high‐performance liquid chromatography method to measure lipophilic antioxidants in stressed plants: simultaneous determination of carotenoids and tocopherols
J. I. García-Plazaola (1999)
10.1002/EJLT.201400656
New approach for vitamin E extraction in rainbow trout flesh: Application in fish fed commercial and red seaweed-supplemented diets: Vitamin E in rainbow trout flesh
Mariana Araújo (2015)
10.1016/J.JCHROMB.2005.06.028
A fast, sensitive method for the simultaneous determination of α-tocopherol and α-tocopheryl acetate in mixed micelles
Stéphane Castan (2005)
10.1556/AVET.53.2005.4.3
Effect of α-tocopherol on antioxidant enzyme activities and lipid peroxidation in rainbow trout (Oncorhynchus mykiss)
İ. Gülçin (2005)
10.1111/ANU.12123
The effect of dietary oxidized lipid levels on growth performance, antioxidant enzyme activities, intestinal lipid deposition and skeletogenesis in Senegalese sole (Solea senegalensis) larvae
Anaïs Boglino (2014)
10.1111/JWAS.12375
Survival and Growth Improvement of Palm Ruff, Seriolella violacea, Larvae Fed Artemia Nauplii Enriched with an Experimental Emulsion
Adens González (2017)
10.1016/S0044-8486(01)00698-6
Use of the brine shrimp, Artemia spp., in marine fish larviculture.
P. Sorgeloos (2001)
10.1016/J.CBPB.2004.06.003
Vitamin E in early stages of sea bass (Dicentrarchus labrax) development.
G. Guerriero (2004)
10.1577/A06-083.1
Influence of Stress and Dietary Natural-Source Vitamin E on Nonspecific Immunocompetence, Tissue Tocopherol Composition, and Postslaughter Fillet Oxidative Stability in Sunshine Bass
J. Trushenski (2007)
10.1016/S0378-4347(99)00013-4
Simultaneous determination of alpha-tocopheryl acetate and tocopherols in aquatic organisms and fish feed.
J. Huo (1999)
10.1111/J.1749-7345.2008.00179.X
Influence of Vitamin E Source and Dietary Supplementation Level on Production Performance of Sunshine Bass, Morone chrysops ♀ × Morone saxatilis ♂, Fillet Tocopherol Content, and Immunocompetency during Stress and Bacterial Challenge
J. Trushenski (2008)
10.1016/S0021-9673(01)01101-3
Chromatographic analysis of α-tocopherol and related compounds in various matrices
F. J. Rupérez (2001)
10.1007/S00216-006-0666-0
Near-infrared spectroscopy (NIRS) reflectance technology for the determination of tocopherols in alfalfa
I. González-Martín (2006)
10.1023/A:1014292223946
Antioxidant effect of dietary tocopherol and ascorbic acid on growth and survival of Litopenaeus vannamei postlarvae
N. Ruff (2004)
10.1111/J.1745-4557.2010.00336.X
GROWTH RESPONSE AND MUSCLE LIPID QUALITY OF INDIAN WHITE SHRIMP FED DIFFERENT OILS AT TWO DIETARY LIPID LEVELS
Hossein Ouraji (2010)
10.1007/978-1-4615-0075-9_68
Oxidative defenses in the sea bass, Dicentrarchus labrax.
G. Guerriero (2003)
Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream ( Sparus aurata L . , Osteichthyes , Sparidae ) fed dietary oxidised oil : Attenuation by dietary vitamin
G. Mourente (2011)
10.1046/J.1365-2095.2002.00205.X
Effects of dietary vitamin E on antioxidant defence mechanisms of juvenile turbot (Scophthalmus maximus L.), halibut (Hippoglossus hippoglossus L.) and sea bream (Sparus aurata L.)
D. Tocher (2002)
10.1111/ANU.12260
Effects of enriched Artemia by fish and soybean oils supplemented with vitamin E on growth performance, lipid peroxidation, lipase activity and fatty acid composition of Persian sturgeon (Acipenser persicus) larvae
Abdolmohammad Abedian Kenari (2016)
10.1590/S0100-204X2008000800016
Palmitato de ascorbil e acetato de tocoferol como antioxidantes metabólicos em larvas de dourado
Daniel Okamura (2008)
10.1023/A:1024127003997
Comparative study of antioxidant defence mechanisms in marine fish fed variable levels of oxidised oil and vitamin E
D. Tocher (2004)
10.1016/j.chroma.2009.02.088
Ultra-High-Pressure Liquid Chromatographic method for the analysis of tocopherols in human colostrum and milk.
C. Moltó-Puigmartí (2009)
10.2983/0730-8000(2006)25[589:EODLOD]2.0.CO;2
EFFECT OF DIFFERENT LEVELS OF DIETARY α-TOCOPHEROL ON OVARIAN MATURATION AND REPRODUCTIVE PERFORMANCE OF BROODSTOCK LITOPENAEUS VANNAMEI (BOONE)
Shaobo Du (2006)
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