Online citations, reference lists, and bibliographies.

Carbohydrate-based Probes For Detection Of Cellular Lectins.

O. Galanina, A. Tuzikov, E. Rapoport, J. Le Pendu, N. Bovin
Published 1998 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Carbohydrate (spacered saccharide residue, Glyc) probes with various tags were synthesized as analytical tools for study of cellular lectins, i.e., Glyc-polyacrylamide-3H, Glyc-PAA-biotin, Glyc-PAA-fluorescein (flu), and Glyc-PAA-digoxigenin, where PAA is a soluble polyacrylamide carrier of approximately 30 kDa. Binding of all types of probes, where Glyc is the sialyl Lewis X (SiaLeX) tetrasaccharide or a blank saccharide, was assessed using Chinese hamster ovary (CHO) cells either transfected with the E-selectin cDNA or mock-transfected. High binding of SiaLeX-PAA-3H to E-selectin-transfected cells and absence of binding to control cells (both native and permeabilized) allowed the conclusion that the polyacrylamide carrier and the spacer arm do not contribute significantly to the binding. The biotinylated probe showed a high level of nonspecific binding in cell enzyme-linked assays. A similarly built digoxigenin-labeled probe was significantly better. In flow cytometry assays, the fluorescein probe demonstrated a specific binding to E-selectin-transfected cells of a similar level to that given by an anti-E-selectin antibody. In addition, it could be inhibited by the anti-E-selectin antibody, further demonstrating specificity. Tumors were obtained from nude mice by injection of CHO E-selectin or mock-transfected cells. The fluorescent SiaLeX-PAA-flu probe could bind to tumor sections from E-selectin-positive CHO cells, but not from control CHO cells. These probes can thus be used to reveal specifically complex carbohydrate-binding sites on cells either in culture or on tissue sections.
This paper references
Further refinement of the description of the ligand-binding characteristics for the galactoside-binding mistletoe lectin, a plant agglutinin with immunomodulatory potency.
O. Galanina (1997)
Labelling of haptenic drug with digoxigenin for competitive immunoassay: its application to lesopitron, a new anxiolytic agent.
A. Adam (1996)
Adhesion of human uroepithelial cells to E‐selectin: Possible involvement of sialosyl LewisA‐ganglioside
A. Kłopocki (1996)
Age-related patterns of lectin-binding nuclear glycoproteins of hamster liver
A. Krześlak (1996)
Leukocyte-endothelial adhesion molecules.
T. Carlos (1994)
Cell Surface Carbohydrates and Cell Development
M. Fukuda (1991)
An E-selectin binding assay based on a polyacrylamide-type glycoconjugate.
G. Weitz-Schmidt (1996)
Increase of rat colon carcinoma cells tumorigenicity by alpha(1-2) fucosyltransferase gene transfection.
C. Goupille (1997)
Detection of a potential receptor for the H‐blood‐group antigen on rat colon‐carcinoma cells and normal tissues
O. Galanina (1998)
Biotinylation of proteins via amino groups can induce binding to U937 cells, HL-60 cells, monocytes and granulocytes.
D. Storm (1996)
Scintillation proximity assay for E-, P-, and L-selectin utilizing polyacrylamide-based neoglycoconjugates as ligands.
S. Game (1998)

This paper is referenced by
Microscale synthesis of glycoconjugate series and libraries.
E. A. Gordeeva (2000)
The synthesis of biotinylated carbohydrates as probes for carbohydrate-recognizing proteins.
D. Angus (2000)
Boronate-containing copolymers: polyelectrolyte properties and sugar-specific interaction with agarose gel.
Marina V. Kuzimenkova (2006)
Receptor binding surveillance of influenza clinical isolates
Ya Wang (2012)
Fluorescent carbohydrate probes for cell lectins.
O. Galanina (2001)
Specificity of human galectins on cell surfaces
Eugenia M. Rapoport (2015)
Affinity of low molecular weight fucoidan for P-selectin triggers its binding to activated human platelets.
Laure Bachelet (2009)
Glycoprobes as a tool for the study of lectins expressed on tumor cells.
Olga Kurmyshkina (2010)
Probing Cell Surface Lectins with Neoglycoconjugates
Eugenia M. Rapoport (2007)
Fishing for lectins from diverse sequence libraries by yeast surface display - an exploratory study.
Stefan Ryckaert (2008)
Real-time monitoring of the cell agglutination process with a quartz crystal microbalance.
Liang Tan (2008)
Sulfated Lewis A trisaccharide on oviduct membrane glycoproteins binds bovine sperm and lengthens sperm lifespan
Sudipta Dutta (2019)
LewisX-Containing Glycans on the Porcine Oviductal Epithelium Contribute to Formation of the Sperm Reservoir1
S. A. Machado (2014)
An Enzyme-Linked Lectin Assay for α1,3-Galactosyltransferase
L. S. Khraltsova (2000)
Antibody targeted gene transfer to endothelium.
P. Tan (2003)
Involvement of the Galβ1 – 3GalNAcβ structure in the recognition of apoptotic bodies by THP-1 cells
Eugenia M. Rapoport (2003)
Probing sialic acid binding Ig-like lectins (siglecs) with sulfated oligosaccharides
E. Rapoport (2006)
Porcine Sperm Bind to Specific 6-Sialylated Biantennary Glycans to Form the Oviduct Reservoir1
G. Kadirvel (2012)
Short Title: Porcine Oviduct 6-Sialylated Glycans Bind Sperm
Govindasamy Kadirvel (2012)
Coronary flow-induced inotropism is modulated by binding of dextrans to the endothelial luminal surface.
C. Gonzalez-Castillo (2003)
Multiple proteins from ejaculated and epididymal porcine spermatozoa bind glycan motifs found in the oviduct
E. Silva (2014)
Recognition of Lewis x Derivatives Present on Mucins by Flagellar Components of Pseudomonas aeruginosa
A. Scharfman (2001)
Neoglycoconjugates as Probes in Glycobiology
N. Bovin (2003)
Semantic Scholar Logo Some data provided by SemanticScholar