Online citations, reference lists, and bibliographies.

Lenses Of SPARC-null Mice Exhibit An Abnormal Cell Surface-basement Membrane Interface.

Kazumi Norose, Woo Kuen Lo, John I Clark, E. Helene Sage, Chin C. Howe
Published 2000 · Biology, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
SPARC (secreted protein acidic and rich in cysteine) is a matricellular protein involved in cell-matrix interactions. We have shown previously that mice deficient in SPARC develop posterior cortical cataract early in life that progresses to a mature opacity and capsule rupture. To evaluate the primary effects of SPARC deficiency in the lens, we examined the lenses of SPARC-null and wild-type mice by electron microscopy and immunohistochemistry to investigate whether ultrastructural abnormalities occur at the basement membrane (capsule)-lens cell interface in SPARC-null mice. The most notable feature in the lenses of SPARC-null mice, relative to wild-type animals, was the modification of the basal surface of the lens epithelial and fiber cells at the basement membrane (capsule) interface. Electron microscopy revealed numerous filopodial projections of the basal surface of the lens epithelial and fiber cells into the extracellular matrix of the anterior, posterior, and equatorial regions of the lens capsule. In 1 week old precataractous lenses, basal invasive filopodia projecting into the capsule were small and infrequent. Both the size and frequency of these filopodia increased in precataractous 3-4 week old lenses and were prominent in the cataractous 5-6 week old lenses. By rhodamine-phalloidin labeling, we confirmed the presence of basal invasive filopodia projecting into the lens capsule and demonstrated that the projections contained actin filaments. In contrast to the obvious abnormal projections at the interface between the basal surface of the lens epithelial and fiber cells and the lens capsule, the apical and lateral plasma membranes of lens epithelial cells and lens fibers in SPARC-null mice were as smooth as those of wild-type mice. We conclude that the absence of SPARC in the murine lens is associated with a filopodial protrusion of the basal surface of the lens epithelium and differentiating fiber cells into the lens capsule. The altered structures appear prior to the opacification of the lens in the SPARC-null model. These observations are consistent with one or more functions previously proposed for SPARC as a modulator of cell shape and cell-matrix interactions.



This paper is referenced by
COL 4 A 1 and COL 4 A 2 mutations and disease : insights into pathogenic mechanisms and potential therapeutic targets
Debbie S Kuo (2012)
10.1046/j.1440-169x.1999.00443.x
A calcium-binding motif in SPARC/osteonectin inhibits chordomesoderm cell migration during Xenopus laevis gastrulation: evidence of counter-adhesive activity in vivo.
Minh Hang Thi Huynh (1999)
Abnormal fiber end migration in Royal College of Surgeons rats during posterior subcapsular cataract formation
Anita Joy (2010)
10.1172/JCI12939
SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury.
Amy D Bradshaw (2001)
10.1007/s00427-002-0220-9
Evolutionary conservation and association of SPARC with the basal lamina in Drosophila
Nathalie Martinek (2002)
10.1242/jcs.021931
Haemocyte-derived SPARC is required for collagen-IV-dependent stability of basal laminae in Drosophila embryos
Nathalie Martinek (2008)
10.1080/03008200290001050
Novel Functions of the Matricellular Proteins Osteopontin and Osteonectin/SPARC
Jaro Sodek (2002)
10.1093/hmg/dds346
COL4A1 and COL4A2 mutations and disease: insights into pathogenic mechanisms and potential therapeutic targets
Debbie S Kuo (2012)
10.1007/s00427-010-0349-x
Knockdown of SPARC leads to decreased cell–cell adhesion and lens cataracts during post-gastrula development in Xenopus laevis
My-Hang Huynh (2010)
10.1177/002215540305100412
Expression of the Matricellular Protein SPARC in Murine Lens: SPARC Is Necessary for the Structural Integrity of the Capsular Basement Membrane
Qi Yan (2003)
Gene expression changes during cataract progression in Sparc null mice: differential regulation of mouse globins in the lens.
F. C. Mansergh (2004)
10.1016/j.exer.2008.02.011
Mutation of Dock5, a member of the guanine exchange factor Dock180 superfamily, in the rupture of lens cataract mouse.
Natsue Omi (2008)
10.1186/1471-2121-2-15
Impaired wound healing in mice deficient in a matricellular protein SPARC (osteonectin, BM-40)
Amitabha S. Basu (2001)
10.15562/gnc7
Therapeutic Role of Extracellular Matrix (ECM): Understanding Lens Development and Pathological Perspective
Mahdi Ghadimi Hamzekolaii (2015)
10.1002/dvdy.24511
The occhiolino (occ) mutant Zebrafish, a model for development of the optical function in the biological lens.
Masamoto Aose (2017)
10.1016/j.exer.2007.11.019
Automated, computerized, feature-based phenotype analysis of slit lamp images of the mouse lens.
Jenny Yuen (2008)
10.1097/01.LAB.0000042240.52093.0F
Neurologic Defects and Selective Disruption of Basement Membranes in Mice Lacking Entactin-1/Nidogen-1
Lijin Dong (2002)
10.1017/erm.2012.3
Antiangiogenic and anticancer molecules in cartilage.
Debabrata Patra (2012)
10.1093/hmg/11.5.507
A Gja8 (Cx50) point mutation causes an alteration of alpha 3 connexin (Cx46) in semi-dominant cataracts of Lop10 mice.
Bo Chang (2002)
Anterior segment alterations and comparative aqueous humor proteomics in the buphthalmic rabbit (an American Ophthalmological Society thesis).
Deepak P. Edward (2011)
10.1007/s00418-006-0200-7
Secreted modular calcium-binding protein-1 localization during mouse embryogenesis
Nikolaus Gersdorff (2006)
10.1371/journal.pone.0077575
Secreted Protein Acidic and Rich in Cysteine (SPARC) Exacerbates Colonic Inflammatory Symptoms in Dextran Sodium Sulphate-Induced Murine Colitis
Yoke-Leng Ng (2013)
Mobilization of Adipose Stromal Cells in Obesity and Cancer by SPARC and its Proteolytic Isoforms
Chieh Tseng (2015)
10.1016/j.ydbio.2017.09.037
Collagen IV trafficking: The inside-out and beyond story.
Alexa Chioran (2017)
10.4161/cc.9.21.13742
New insights into 5q- syndrome as a ribosomopathy
Jillian L Barlow (2010)
10.1167/iovs.05-0460
Absence of SPARC in murine lens epithelium leads to increased deposition of laminin-1 in lens capsule.
Qi Yan (2005)
10.3892/etm.2018.6970
Glucose affects cell viability, migration, angiogenesis and cellular adhesion of human retinal capillary endothelial cells via SPARC
Yang Fu (2019)
10.1177/154405910708600402
Is SPARC an Evolutionarily Conserved Collagen Chaperone?
Nathalie Martinek (2007)
10.1042/BJ20030532
Characterization of SMOC-2, a modular extracellular calcium-binding protein.
Christian Vannahme (2003)
10.1089/jop.2015.0057
Secreted Protein Acidic and Rich in Cysteine in Ocular Tissue.
Kurt Scavelli (2015)
Semantic Scholar Logo Some data provided by SemanticScholar