Online citations, reference lists, and bibliographies.

Immunization With VP2 Is Sufficient For Protection Against Lethal Challenge With African Horsesickness Virus Type 4.

M. Stone-Marschat, S. R. Moss, T. Burrage, M. Barber, P. Roy, W. Laegreid
Published 1996 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Horses were immunized by inoculation with a vaccinia construct containing a full-length cDNA corresponding to the L2 gene segment of African horsesickness virus type 4(AHSV-4). All immunized horses developed serum neutralizing antibodies prior to challenge with virulent AHSV-4. No ELISA-reactive antibodies were present prior to challenge. A group of four seronegative control horses died after developing clinical signs and lesions typical of the pulmonary form of African horsesickness while the immunized horses were clinically normal. Increases in serum neutralizing and ELISA-reactive antibody titers following challenge indicate that at least some replication of challenge virus occurred in immunized horses. These results demonstrate that AHSV VP2 alone is sufficient to induce a protective immune response in horses and indicate the usefulness of ELISA-reactive antibodies for differentiation of vaccinated and naturally exposed horses.

This paper is referenced by
Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes.
A. Potgieter (2002)
Development of a mouse model system, coding assignments and identification of the genome segments controlling virulence of African horse sickness virus serotypes 3 and 8.
R. Ohara (1998)
Detection of African horsesickness virus and discrimination between two equine orbivirus serogroups by reverse transcription polymerase chain reaction.
C. Bremer (1998)
African horse sickness virus serotype 4 antigens, VP1-1, VP2-2, VP4, VP7 and NS3, induce cytotoxic T cell responses in vitro.
F E Faber (2016)
The protective efficacy of a recombinant VP2-based African horsesickness subunit vaccine candidate is determined by adjuvant.
M. Scanlen (2002)
Cloning viral dsRNA genomes : Analysis and application
C. A. (2004)
Standardization and validation of an immunoperoxidase test for African horsesickness virus using formalin-fixed, paraffin-embedded tissues
Sarah J Clift (2011)
Immunogenicity of recombinant VP2 proteins of all nine serotypes of African horse sickness virus
Y. Kanai (2014)
Protective immunization of horses with a recombinant canarypox virus vectored vaccine co-expressing genes encoding the outer capsid proteins of African horse sickness virus.
A. Guthrie (2009)
Tubules composed of non-structural protein NS1 of african horsesickness virus as system for the immune display of foreign peptides
Karen Lacheiner (2010)
Molecular epidemiology of the African horse sickness virus S10 gene.
M. Quan (2008)
Antiserum from mice vaccinated with modified vaccinia Ankara virus expressing African horse sickness virus (AHSV) VP2 provides protection when it is administered 48 h before, or 48 h after challenge
Eva Calvo-Pinilla (2015)
Cloning, sequencing and expression of the gene that encodes the major neutralisation-specific antigen of African horsesickness virus serotype 9.
M. Venter (2000)
An African horse sickness virus serotype 4 recombinant canarypox virus vaccine elicits specific cell-mediated immune responses in horses.
H. El Garch (2012)
Variation in African Horse Sickness Virus and its Effect on the Vector Competence of Culicoides Biting Midges
L. Riegler (2002)
Suppression of African horse sickness virus NS1 protein expression in mammalian cells by short hairpin RNAs
Helena Roos (2009)
African horse sickness virus dynamics and host responses in naturally infected horses
Camilla Theresa Weyer (2011)
Solubility, particle formation and immune display of trimers of major capsid protein 7 of African horsesickness virus fused with enhanced green fluorescent protein
E. Mizrachi (2011)
Vaccination of horses with a recombinant modified vaccinia Ankara virus (MVA) expressing African horse sickness (AHS) virus major capsid protein VP2 provides complete clinical protection against challenge
Berta Alberca (2014)
Immune responses to modified-live and recombinant African horse sickness virus vaccines
Jan E Crafford (2014)
Tick-borne Great Island Virus: (II) Impact of age-related acquired immunity on transmission in a natural seabird host.
M. Nunn (2006)
The characterization of inner core protein VP6 of African horsesickness virus
De Waal (2007)
Experiences with new generation vaccines against equine viral arteritis, West Nile disease and African horse sickness.
N. J. Maclachlan (2007)
An evaluation of the vaccine-vector potential of thymidine kinase-disrupted recombinants of lumpy skin disease virus (South African vaccine)
D. Wallace (2007)
Vaccination of mice with a modified Vaccinia Ankara (MVA) virus expressing the African horse sickness virus (AHSV) capsid protein VP2 induces virus neutralising antibodies that confer protection against AHSV upon passive immunisation.
Eva Calvo-Pinilla (2014)
Characterisation and co-expression of the two outer capsid proteins of African horsesickness virus serotype 3
Renate Dorothea Filter (2006)
An experimental subunit vaccine based on Bluetongue virus 4 VP2 protein fused to an antigen-presenting cells single chain antibody elicits cellular and humoral immune responses in cattle, guinea pigs and IFNAR(-/-) mice.
Danilo Mario Legisa (2015)
Immune responses in a horse inoculated with the VP2 gene of African horsesickness virus.
M. Romito (1999)
Silencing of African horse sickness virus NS2 gene expression using vector-derived short hairpin RNAs
Marthi Nieuwoudt (2010)
Virus-specific CD8⁺ T-cells detected in PBMC from horses vaccinated against African horse sickness virus.
Alri Pretorius (2012)
Immune gene expression profiling of PBMC isolated from horses vaccinated with attenuated African horsesickness virus serotype 4.
Alri Pretorius (2016)
Construction of a new peptide insertion site in the top domain of major core protein VP7 of African horsesickness virus
J. Riley (2005)
See more
Semantic Scholar Logo Some data provided by SemanticScholar