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The Structure Of The Mer Operon.

P. Barrineau, P. Gilbert, W. Jackson, C. Jones, A. O. Summers, S. Wisdom
Published 1985 · Biology, Medicine

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The DNA sequence has been determined for a 3.8-kb region which encodes the mercury-resistance (mer) operon of the IncFII plasmid NR1. The sequence reveals 4 open reading frames which could encode proteins of 12,522, 9,429, 14,965, and 58,912 d corresponding to the 4 previously described Hg-inducible proteins detected in minicells carrying mer+ plasmids. The Hg(II) reductase protein sequence is about 90% homologous to that of Tn501, but the DNA sequence shows a homology of 60-70% to that of Tn501 except for short regions of very high homology. The entire mer region is 63.4% G-C overall. The region encoding the merR (positive regulatory) function has 3 possible open reading frames, 2 of which overlap in one direction and the third of which reads in the opposite direction. Attempts to visualize the polypeptide(s) encoded by the merR cistron were unsuccessful.
This paper references
10.1128/JB.140.1.161-166.1979
Hypersensitivity to Hg2+ and hyperbinding activity associated with cloned fragments of the mercurial resistance operon of plasmid NR1.
H. Nakahara (1979)
The DNA sequence of the mercury resistance operon of the IncFII plasmid NR1.
P. Barrineau (1984)
10.1128/MMBR.47.3.361-409.1983
Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria.
T. J. Foster (1983)
10.1016/0076-6879(83)01005-8
[2] New M13 vectors for cloning
J. Messing (1983)
10.1128/JB.151.1.222-228.1982
Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1.
F. de la Cruz (1982)
10.1016/0092-8674(81)90178-1
Transposon-mediated site-specific recombination: A defined in vitro system
R. Reed (1981)
10.1016/S0147-619X(80)90032-3
Transposition of mercury resistance from a transferable R plasmic of Escherichia coli.
A. Summers (1980)
10.1146/ANNUREV.MI.32.100178.003225
Microbial transformations of metals.
A. Summers (1978)
10.1073/pnas.74.12.5463
DNA sequencing with chain-terminating inhibitors.
F. Sanger (1977)
10.1128/AAC.22.5.852
Selection for mercurial resistance in hospital settings.
F. Porter (1982)
10.1021/BI00286A014
Mercuric reductase: homology to glutathione reductase and lipoamide dehydrogenase. Iodoacetamide alkylation and sequence of the active site peptide.
B. Fox (1983)
10.1271/BBB1961.36.2441
Induction of Metallic Mercury-releasing Enzyme in Mercury-resistant Pseudomonas
K. Furukawa (1972)
10.1128/JB.155.2.690-703.1983
Tn5 insertion mutations in the mercuric ion resistance genes derived from plasmid R100.
N. Nibhriain (1983)
The mercuric and organomercurial detoxifying enzymes from a plasmid-bearing strain of Escherichia coli.
J. Schottel (1978)
10.1128/JB.147.3.1110-1112.1981
Translocatable resistance to mercuric and phenylmercuric ions in soil bacteria.
A. Radford (1981)
10.1016/0022-2836(82)90515-0
A simple method for displaying the hydropathic character of a protein.
J. Kyte (1982)
10.1016/0147-619X(79)90020-9
Transcription and translation of R-plasmid 538-1 DNA: effects of mercury induction and analysis of polypeptides coded for by the r-determinant region.
N. K. Alton (1979)
10.1128/JB.127.1.619-636.1976
EcoRI restriction endonuclease map of the composite R plasmid NR1.
N. Tanak (1976)
10.1128/JB.140.1.167-181.1979
Transposon A-generated mutations in the mercuric resistance genes of plasmid R100-1.
T. Foster (1979)
10.1021/BI00286A015
Nucleotide sequence of a gene from the Pseudomonas transposon Tn501 encoding mercuric reductase.
N. Brown (1983)
10.1128/JB.149.2.479-487.1982
Polypeptides encoded by the mer operon.
W. Jackson (1982)
10.1016/0378-1119(83)90225-1
A second positive regulatory function in the mer (mercury resistance) operon.
P. Barrineau (1983)
10.1128/JB.151.2.962-970.1982
Biochemical characterization of HgCl2-inducible polypeptides encoded by the mer operon of plasmid R100.
W. Jackson (1982)
10.1073/PNAS.71.4.1342
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
J. Shine (1974)
10.1021/BI00273A025
Mercuric reductase from R-plasmid NR1: characterization and mechanistic study.
S. J. Rinderle (1983)
10.1073/PNAS.72.3.784
Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
D. Pribnow (1975)
10.1128/JB.129.3.1227-1233.1977
Characterization of a translocation unit encoding resistance to mercuric ions that occurs on a nonconjugative plasmid in Pseudomonas aeruginosa.
V. Stanisich (1977)
10.1016/0378-1119(81)90182-7
Cloning a promoter that puts the expression of tetracycline resistance under the control of the regulatory elements of the mer operon.
F. Bohlander (1981)
10.1016/s0021-9258(18)34951-2
Mercuric reductase. Purification and characterization of a transposon-encoded flavoprotein containing an oxidation-reduction-active disulfide.
B. Fox (1982)



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Intracellular Pb 2 þ Content Monitoring Using a Protein-Based Pb 2 þ Indicator
Tai-Yu Chiu (2012)
10.1093/toxsci/kfs007
Intracellular Pb2+ content monitoring using a protein-based Pb2+ indicator.
Tai-Yu Chiu (2012)
10.1038/71986
Engineering Deinococcus radiodurans for metal remediation in radioactive mixed waste environments
Hassan Brim (2000)
10.1007/BF00871630
The FNR family of transcriptional regulators
S. Spiro (2004)
Hassan Brim , Amudhan Venkateswaran , Heather M . Radioactive Waste Environments Bioremediation of High-Temperature for Deinococcus geothermalis
Hassan Brim (2003)
Studies on gene expression in a pathogenic bacterium
S. Islam (2011)
10.1128/AEM.69.8.4575-4582.2003
Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments
Hassan Brim (2003)
10.1016/S0168-6445(03)00051-2
The MerR family of transcriptional regulators.
N. Brown (2003)
10.1128/AEM.63.3.1066-1076.1997
Phylogeny of mercury resistance (mer) operons of gram-negative bacteria isolated from the fecal flora of primates.
C. Liebert (1997)
10.2741/1447
Sensing and adapting to environmental stress: the archaeal tactic.
E. Pedone (2004)
10.3389/fmicb.2015.00648
Transcription factor-based biosensors enlightened by the analyte
R. Fernández-López (2015)
10.1007/s11274-017-2279-5
Radiation resistance in thermophiles: mechanisms and applications
P. Ranawat (2017)
Structural studies of putative general stress and related proteins from Deinococcus radiodurans
Claire Louise Waterston (2009)
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