← Back to Search
Identification And In Situ Detection Of Intracellular Bacteria In The Environment.
B. Brand, R. Amann, M. Steinert, D. Grimm, J. Hacker
Published 2000 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Today it is generally accepted that our knowledge of bacterial diversity in the environment has been severely limited by the need to obtain pure cultures prior to characterization by testing for multiple physiological and biochemical properties. In addition, the morphology of microorganisms is in general too simple to serve as a basis for a reliable and proper classification; only in rare cases does it allow the in situ identification of individual population members by microscopy (Woese, 1987). Viable plate count or most probable-number techniques have been used for quantification of active cells in different environments but are always selective and can therefore not yield sufficient documentation of the true community structure (Table 1). For aquatic habitats as well as soils and sediments it has been frequently reported that direct microscopic counts exceed viable-cell counts by several orders of magnitude (Torsvik et al.,1990; Ferguson et al.,1984; Jones, 1977). This phenomenon is known as the “great plate count anomaly” described by Staley and Konopka (1985). Any estimation of the numbers of bacteria in the environment, whether they are pathogens, indicator organisms or genetically modified microorganisms, must allow for the fact that a proportion of the target organisms have entered the non-culturable but viable fraction of the microbial population. This accounts especially for bacterial endosymbionts colonizing free-living and parasitic protozoa although the roles such endosymbionts play in host survial, infectivity, and invasiveness are unclear (Fritsche et al., 1993).
This paper references
Response of marine bacterioplankton to differential filtration and confinement.
R. Ferguson (1984)
Fluorescently labelled, rRNA‐targeted oligonucleotide probes in the study of microbial ecology
R. Amann (1995)
Bacterial entry into epithelial cells: the paradigm of Shigella.
R. Ménard (1996)
Host Response to Intracellular Pathogens
S. Kaufmann (1997)
Comparison of the hydrophobic grid-membrane filter DNA probe method and the Health Protection Branch standard method for the detection of Listeria monocytogenes in foods.
W. Yan (1996)
In Situ Localization of Azospirillum brasilense in the Rhizosphere of Wheat with Fluorescently Labeled, rRNA-Targeted Oligonucleotide Probes and Scanning Confocal Laser Microscopy.
B. Assmus (1995)
Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
R. Amann (1990)
Rapid, universal method to isolate PCR-ready DNA using magnetic beads.
K. Rudi (1997)
In situ visualization of high genetic diversity in a natural microbial community.
R. Amann (1996)
Legionella pneumophila in cooling water systems. Report of a survey of cooling towers in London and a pilot trial of selected biocides.
J. Kurtz (1982)
A new principle for the determination of total bacterial numbers in populations recovered from aerosols.
J. D. Anderson (1967)
Molecular and cellular biology of Shigella flexneri invasiveness: from cell assay systems to shigellosis.
P. Sansonetti (1992)
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
R. Amann (1995)
Identification of single bacterial cells using digoxigenin-labelled, rRNA-targeted oligonucleotides.
B. Zarda (1991)
Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.
D. Stahl (1988)
Infection of Acanthamoeba castellanii by Chlamydia pneumoniae.
A. Essig (1997)
Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized by using episcopic differential interference contrast microscopy.
J. Rogers (1992)
Environmental mycobacteria and human disease.
Grange Jm (1991)
High diversity in DNA of soil bacteria.
V. Torsvik (1990)
The phylogenetic status of Sarcobium lyticum, an obligate intracellular bacterial parasite of small amoebae.
N. Springer (1992)
Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp.
R. Amann (1997)
Listeria monocytogenes, a food-borne pathogen.
J. Farber (1991)
In situ identification of Legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy.
Werner Manz (1995)
A new colorimetric nucleic acid hybridization assay for Listeria in foods.
W. King (1989)
Association of Listeria spp. Contamination in the Dairy Processing Plant Environment with the Presence of Staphylococci.
J. Frank (1990)
Polynucleobacter necessarius, an obligate bacterial endosymbiont of the hypotrichous ciliate Euplotes aediculatus, is a member of the beta-subclass of Proteobacteria.
N. Springer (1996)
Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii.
M. Steinert (1997)
Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion.
J. Cirillo (1994)
Analysis of plasmids in Mycobacterium avium-intracellulare isolates from persons with acquired immunodeficiency syndrome.
J. Crawford (1986)
Prevalence and diagnosis of Legionella pneumonia: a 3-year prospective study with emphasis on application of urinary antigen detection.
B. Ruf (1990)
Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.
E. DeLong (1989)
Ingested Listeria monocytogenes survive and multiply in protozoa.
T. Ly (1990)
RNA sequence analysis shows that the symbionts in the ciliate Metopus contortus are polymorphs of a single methanogen species
T. M. Embley (1992)
The frequency of chimeric molecules as a consequence of PCR co-amplification of 16S rRNA genes from different bacterial species.
G. Wang (1996)
Survival of coliforms and bacterial pathogens within protozoa during chlorination.
C. King (1988)
INTERACTIONS OF LISTERIA MONOCYTOGENES, LISTERIA SEELIGERI, AND LISTERIA INNOCUA WITH PROTOZOANS
Thi Minh Chau Ly (1990)
Long-term survival of Legionella pneumophila serogroup 1 under low-nutrient conditions and associated morphological changes
C. Paszko-Kolva (1992)
Trojan horses of the microbial world: protozoa and the survival of bacterial pathogens in the environment.
J. Barker (1994)
Growth and in situ detection of a pathogenic Escherichia coli in biofilms of a heterotrophic water-bacterium by use of 16S- and 23S-rRNA- directed fluorescent oligonucleotide probes
U. Szewzyk (1994)
The use of rRNA sequences and fluorescent probes to investigate the phylogenetic positions of the anaerobic ciliate Metopus palaeformis and its archaeobacterial endosymbiont.
T. M. Embley (1992)
DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.
W. Holben (1988)
Identification in situ and phylogeny of uncultured bacterial endosymbionts
R. Amann (1991)
Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting.
M. Peel (1988)
ENVIRONMENTAL POLLUTION BY CADMIUM AND MORTALITY FROM RENAL DISEASES
R. Lauwerys (1981)
Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.
R. Amann (1990)
Occurrence of fragmented 16S rRNA in an obligate bacterial endosymbiont of Paramecium caudatum.
N. Springer (1993)
Phylogenetic Oligodeoxynucleotide Probes for the Major Subclasses of Proteobacteria: Problems and Solutions
Werner Manz (1992)
Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.
E. D. Kopczynski (1994)
Transcriptional regulation of the macrophage‐induced gene (gspA) of Legionella pneumophila and phenotypic characterization of a null mutant
Yousef Abu Kwaik (1997)
Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure.
M. Wagner (1993)
Acanthamoeba from human nasal mucosa infected with an obligate intracellular parasite
R. Michel (1994)
Epidemiology of infection by nontuberculous mycobacteria.
J. Rd (1996)
Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms.
G. Wallner (1993)
In situ probing of gram-positive bacteria with high DNA G + C content using 23S rRNA-targeted oligonucleotides.
C. Roller (1994)
Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes.
A. Schramm (1996)
Intraphagocytic growth induces an antibiotic-resistant phenotype of Legionella pneumophila.
J. Barker (1995)
Simple, rapid method for direct isolation of nucleic acids from aquatic environments.
C. Somerville (1989)
Genus- and group-specific hybridization probes for determinative and environmental studies of sulfate-reducing bacteria
R. Devereux (1992)
Microbial ecology and evolution: a ribosomal RNA approach.
G. Olsen (1986)
The RDP-II (Ribosomal Database Project)
B. Maidak (2001)
Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes.
R. Amann (1992)
Epidemiology of Shigella dysenteriae, type 1 infections, in Dacca urban area.
M. Khan (1979)
Isolation of protozoa from water associated with a legionellosis outbreak and demonstration of intracellular multiplication of Legionella pneumophila.
J. Barbaree (1986)
Epidemiology of human listeriosis.
A. Schuchat (1991)
Isolation of atypical mycobacteria from tap water in hospitals and homes: is this a possible source of disseminated MAC infection in AIDS patients?
M. Peters (1995)
Interaction of Mycobacterium avium with environmental amoebae enhances virulence.
J. Cirillo (1997)
A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea-pigs.
R. Jepras (1985)
Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR.
M. Briglia (1996)
Increase in Fluorescence Intensity of 16S rRNA In Situ Hybridization in Natural Samples Treated with Chloramphenicol.
C. Ouverney (1997)
Specific Detection of Legionella pneumophila: Construction of a New 16S rRNA-Targeted Oligonucleotide Probe
D. Grimm (1998)
Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective.
R. Gutell (1994)
The effect of environmental factors on estimated viable and total populations of planktonic bacteria in lakes and experimental enclosures
J. Jones (1977)
Use of the polymerase chain reaction and fluorescent-antibody methods for detecting viable but nonculturable Shigella dysenteriae type 1 in laboratory microcosms.
M. Islam (1993)
Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms.
L. Poulsen (1993)
Legionnaires' disease bacterium (Legionella pneumophila) multiples intracellularly in human monocytes.
M. Horwitz (1980)
LISTERIA MONOCYTOGENES AND CHILLED FOODS
R. Gilbert (1989)
Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.
W. Schönhuber (1997)
Mycobacterium avium Bacilli Grow Saprozoically in Coculture with Acanthamoeba polyphaga and Survive within Cyst Walls
M. Steinert (1998)
Detection of micro-organisms in soil after in situ hybridization with rRNA-targeted, fluorescently labelled oligonucleotides.
D. Hahn (1992)
Polymerase chain reaction strategy.
N. Arnheim (1992)
In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes.
M. Wagner (1998)
Viable Legionella pneumophila Not Detectable by Culture on Agar Media
D. Hussong (1987)
Evaluation of luciferase reporter bacteriophage A511::luxAB for detection of Listeria monocytogenes in contaminated foods.
M. Loessner (1997)
Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-fed biofilms.
Alexander Neef (1996)
In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S rRNA-directed fluorescent oligonucleotide probes.
Werner Manz (1993)
Potential virulence of viable but nonculturable Shigella dysenteriae type 1.
I. Rahman (1996)
In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes
R. Amann (1995)
An in situ hybridization protocol for detection and identification of planktonic bacteria
F. Glöckner (1996)
Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses.
T. Fritsche (1993)
Distribution of sulfate-reducing bacteria, O2, and H2S in photosynthetic biofilms determined by oligonucleotide probes and microelectrodes.
N. Ramsing (1993)
An outbreak of Shiga bacillus dysentery in KwaZulu/Natal, South Africa.
D. Pillay (1997)
Influence of site specifically altered Mip proteins on intracellular survival of Legionella pneumophila in eukaryotic cells.
E. Wintermeyer (1995)
Susceptibility of Starved Planktonic and Biofilm Listeria monocytogenes to Quaternary Ammonium Sanitizer as Determined by Direct Viable and Agar Plate Counts.
T. Ren (1993)
Immunomagnetic separation and PCR detection show Shigellae to be common faecal agents in children from urban marginal communities of Costa Rica.
R. Achí (1996)
DNA polymerase fidelity and the polymerase chain reaction.
K. Eckert (1991)
7 Labelled-Antibody Methods for Detection and Identification of Microorganisms
S. Chantler (1988)
Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats.
J. Staley (1985)
Chlamydia-like obligate parasite of free-living amoebae
R. Birtles (1997)
Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.
R. Amann (1992)
Database on the structure of large ribosomal subunit RNA
P. D. Rijk (1996)
This paper is referenced by
Polynucleotide probe based, taxon-specific detection and enrichment of fecal bacteria
L. M. Bruder (2016)
Analysis of microbial diversity in an extreme environment: White Island, New Zealand
R. Ibáñez-Peral (2009)
Bacteria and Inflammatory Cells in Fetal Membranes Do Not Always Cause Preterm Labor
J. Steel (2005)
Legionellosis, a disease transmitted by technical vectors.
M. Steinert (2001)
Legionella pneumophila: an aquatic microbe goes astray.
M. Steinert (2002)