Online citations, reference lists, and bibliographies.
← Back to Search

Molecular Properties Of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing By PCR And Real-Time RT-PCR.

C. Burns, D. Kilpatrick, J. Iber, Qi Chen, O. Kew
Published 2016 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Virologic surveillance is essential to the success of the World Health Organization initiative to eradicate poliomyelitis. Molecular methods have been used to detect polioviruses in tissue culture isolates derived from stool samples obtained through surveillance for acute flaccid paralysis. This chapter describes the use of realtime PCR assays to identify and serotype polioviruses. In particular, a degenerate, inosine-containing, panpoliovirus (panPV) PCR primer set is used to distinguish polioviruses from NPEVs. The high degree of nucleotide sequence diversity among polioviruses presents a challenge to the systematic design of nucleic acid-based reagents. To accommodate the wide variability and rapid evolution of poliovirus genomes, degenerate codon positions on the template were matched to mixed-base or deoxyinosine residues on both the primers and the TaqMan™ probes. Additional assays distinguish between Sabin vaccine strains and non-Sabin strains. This chapter also describes the use of generic poliovirus specific primers, along with degenerate and inosine-containing primers, for routine VP1 sequencing of poliovirus isolates. These primers, along with nondegenerate serotype-specific Sabin primers, can also be used to sequence individual polioviruses in mixtures.
This paper references
Cross antigenicity among enteroviruses as revealed by immunoblot technique.
T. Mertens (1983)
Molecular epidemiology of polioviruses.
O. Kew (1984)
Applications of Oligonucleotide Fingerprinting to the Identification of Viruses
O. Kew (1984)
Antigenic conservation and divergence between the viral-specific proteins of poliovirus type 1 and various picornaviruses.
E. Emini (1985)
Antigenic and molecular evolution of the vaccine strain of type 3 poliovirus during the period of excretion by a primary vaccinee.
P. Minor (1986)
Polymerase chain reaction for human picornaviruses.
T. Hyypiä (1989)
Detection and differentiation of picornaviruses in clinical samples following genomic amplification.
D. M. Olive (1990)
Optimization of the annealing temperature for DNA amplification in vitro.
W. Rychlik (1990)
Detection and identification of vaccine-related polioviruses by the polymerase chain reaction.
C. Yang (1991)
Oligonucleotide probes for the specific detection of the wild poliovirus types 1 and 3 endemic to Brazil.
E. E. da Silva (1991)
The natural genomic variability of poliovirus analyzed by a restriction fragment length polymorphism assay.
J. Balanant (1991)
Genotype-specific in vitro amplification of sequences of the wild type 3 polioviruses from Mexico and Guatemala.
C. Yang (1992)
Shedding of virulent poliovirus revertants during immunization with oral poliovirus vaccine after prior immunization with inactivated polio vaccine.
R. Abraham (1993)
Soluble receptor‐resistant poliovirus mutants identify surface and internal capsid residues that control interaction with the cell receptor.
E. Colston (1994)
Selective isolation of poliovirus in recombinant murine cell line expressing the human poliovirus receptor gene.
T. Hovi (1994)
Paralytic poliomyelitis: seasoned strategies, disappearing disease
H. Hull (1994)
Comparative study of five methods for intratypic differentiation of polioviruses.
H. van der Avoort (1995)
Identification of vaccine-related polioviruses by hybridization with specific RNA probes.
L. De (1995)
Reverse transcription multiplex PCR for differentiation between polio- and enteroviruses from clinical and environmental samples.
D. Egger (1995)
Serotype-Specific Identification of Polioviruses by PCR Using Primers Containing Mixed-Base or Deoxyinosine Residues at Positions of Codon Degeneracy
D. Kilpatrick (1998)
Evolution of the Sabin Strain of Type 3 Poliovirus in an Immunodeficient Patient during the Entire 637-Day Period of Virus Excretion
Javier Martín (2000)
Outbreak of Poliomyelitis in Hispaniola Associated with Circulating Type 1 Vaccine-Derived Poliovirus
O. Kew (2002)
Circulation of Type 1 Vaccine-Derived Poliovirus in the Philippines in 2001
H. Shimizu (2004)
Differentiation of vaccine and wild-type polioviruses using polymerase chain reaction and restriction enzyme analysis
B. Schweiger (2005)
Vaccine-derived polioviruses and the endgame strategy for global polio eradication.
O. Kew (2005)
Antigenic Evolution of Vaccine-Derived Polioviruses: Changes in Individual Epitopes and Relative Stability of the Overall Immunological Properties
M. L. Yakovenko (2006)
Calibration of Multiple Poliovirus Molecular Clocks Covering an Extended Evolutionary Range
J. Jorba (2008)
Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues
D. Kilpatrick (2009)
Recombination between Polioviruses and Co-Circulating Coxsackie A Viruses: Role in the Emergence of Pathogenic Vaccine-Derived Polioviruses
S. Jégouic (2009)
Poliovirus serotype-specific VP1 sequencing primers.
D. Kilpatrick (2011)
Multiple Independent Emergences of Type 2 Vaccine-Derived Polioviruses during a Large Outbreak in Northern Nigeria
C. C. Burns (2013)
Identification of vaccine-derived polioviruses using dual-stage real-time RT-PCR.
D. Kilpatrick (2014)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar