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Surface Profiling Of Extracellular Vesicles From Plasma Or Ascites Fluid Using DotScan Antibody Microarrays.
L. Belov, S. Hallal, Kieran J Matic, J. Zhou, S. Wissmueller, N. Ahmed, Sumaiya Tanjil, S. Mulligan, O. Best, R. Simpson, R. Christopherson
Published 2017 · Chemistry, Medicine
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DotScan antibody microarrays were initially developed for the extensive surface profiling of live leukemia and lymphoma cells. DotScan's diagnostic capability was validated with an extensive clinical trial using mononuclear cells from the blood or bone marrow of leukemia or lymphoma patients. DotScan has also been used for the profiling of surface proteins on peripheral blood mononuclear cells (PBMC) from patients with HIV, liver disease, and stable and progressive B-cell chronic lymphocytic leukemia (CLL). Fluorescence multiplexing allowed the simultaneous profiling of cancer cells and leukocytes from disaggregated colorectal and melanoma tumor biopsies after capture on DotScan. In this chapter, we have used DotScan for the surface profiling of extracellular vesicles (EV) recovered from conditioned growth medium of cancer cell lines and the blood of patients with CLL. The detection of captured EV was performed by enhanced chemiluminescence (ECL) using biotinylated antibodies that recognized antigens expressed on the surface of the EV subset of interest. DotScan was also used to profile EV from the blood of healthy individuals and the ascites fluid of ovarian cancer patients. DotScan binding patterns of EV from human plasma and other body fluids may yield diagnostic or prognostic signatures for monitoring the incidence, treatment, and progression of cancers.
This paper references
Sialylation of N-Linked Glycans Influences the Immunomodulatory Effects of IgM on T Cells
M. Colucci (2015)
Identity of the elusive IgM Fc receptor (FcμR) in humans
H. Kubagawa (2009)
An extended antibody microarray for surface profiling metastatic melanoma.
Kimberley Louise Kaufman Alexander-Kaufman (2010)
Stable and unstable angina: Identifying novel markers on circulating leukocytes
A. Brown (2008)
A new diagnostic tool with the potential to predict tumor metastasis.
J. Nicholas (2013)
Immunohistochemical analysis of beta3 integrin (CD61): expression in pig tissues and human tumors.
A. Meroño (2002)
CEA Serum Levels in Non-Neoplastic Disease
A. R. Morell (1992)
Multicolor flow cytometry for evaluation of platelet surface antigens and activation markers.
Jeroen F van Velzen (2012)
Extracellular vesicles: communication, coercion, and conditioning
David A. Shifrin (2013)
Immunophenotyping of leukemias using a cluster of differentiation antibody microarray.
L. Belov (2001)
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
C. Théry (2006)
Malignant ascites-derived exosomes of ovarian carcinoma patients contain CD24 and EpCAM.
S. Runz (2007)
Nonspecific Immunoglobulin M Binding and Chondroitin Sulfate A Binding Are Linked Phenotypes of Plasmodium falciparum Isolates Implicated in Malaria during Pregnancy
A. Creasey (2003)
Emerging roles of extracellular vesicles in the adaptive response of tumour cells to microenvironmental stress
P. Kucharzewska (2013)
Enhanced Expression of CD13 in Vessels of Inflammatory and Neoplastic Tissues
P. D. Matteo (2011)
Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
C. Gardiner (2013)
The relationship of survival to staging and grading of colorectal carcinoma: A prospective study of 503 cases
R. C. Newland (1981)
Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays.
P. Ellmark (2008)
Disease-specific complications of chronic lymphocytic leukemia.
C. Dearden (2008)
Analysis of human liver disease using a cluster of differentiation (CD) antibody microarray
W. Rahman (2012)
Urinary Extracellular Vesicles as Source of Biomarkers in Kidney Diseases
Ana Gámez-Valero (2015)
Expression of B-cell surface antigens in subpopulations of exosomes released from B-cell lymphoma cells.
M. Oksvold (2014)
Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes
Rossella Crescitelli (2013)
Proteolytic Potential of the MSC Exosome Proteome: Implications for an Exosome-Mediated Delivery of Therapeutic Proteasome
R. C. Lai (2012)
Tumor-derived microvesicles and the cancer microenvironment.
G. Camussi (2013)
Isolation of prostate cancer-related exosomes.
K. Mizutani (2014)
Genetic control of the CD4/CD8 T-cell ratio in humans
A. Amadori (1995)
Activated Platelets Release Two Types of Membrane Vesicles: Microvesicles by Surface Shedding and Exosomes Derived From Exocytosis of Multivesicular Bodies and -Granules
H. F. G. Heijnen (1999)
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
R. Xu (2015)
B cell chronic lymphocytic leukemia: lessons learned from studies of the B cell antigen receptor.
N. Chiorazzi (2003)
Cellular origin and pathophysiology of chronic lymphocytic leukemia
M. Seifert (2012)
Multiplex detection of surface molecules on colorectal cancers
Peter Ellmark (2006)
Contact printing of protein microarrays.
John Austin (2011)
Evaluation of surface chemistries for antibody microarrays.
Shannon L. Seurynck-Servoss (2007)
Colorectal cancer cell surface protein profiling using an antibody microarray and fluorescence multiplexing.
J. Zhou (2011)
Extracellular Vesicles Present in Human Ovarian Tumor Microenvironments Induce a Phosphatidylserine-Dependent Arrest in the T-cell Signaling Cascade
R. J. Kelleher (2015)
Extracellular vesicles as modulators of the cancer microenvironment.
J. Webber (2015)
Multifarious roles of sialic acids in immunity
A. Varki (2012)
Alternative Methods for Characterization of Extracellular Vesicles
F. Momen-Heravi (2012)
Proteomic characterisation of chronic lymphocytic leukaemia cells treated with rituximab
M. Alomari (2014)
Induction of Exosome Release in Primary B Cells Stimulated via CD40 and the IL-4 Receptor1
Sarah C Saunderson (2008)
Exosomes Are Unlikely Involved in Intercellular Nef Transfer
X. Luo (2015)
Tetraspanins in Extracellular Vesicle Formation and Function
Zoraida Andreu (2014)
Profiles of surface mosaics on chronic lymphocytic leukemias distinguish stable and progressive subtypes.
Pauline Y. Huang (2013)
Prevalidation of Salivary Biomarkers for Oral Cancer Detection
D. Elashoff (2012)
Elevated preoperative CEA is associated with worse survival in stage I–III rectal cancer patients
I. Tarantino (2012)
Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles
L. H. Pugholm (2016)
Total lymphocyte CD8 expression is not a reliable marker of cytotoxic T-cell populations in human peripheral blood following an acute bout of high-intensity exercise
J. P. Campbell (2008)
Breast Cancer-Derived Extracellular Vesicles: Characterization and Contribution to the Metastatic Phenotype
T. Green (2015)
Treatment of older patients with chronic lymphocytic leukemia: key questions and current answers.
T. Shanafelt (2013)
Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube.
B. György (2014)
Antibody Microarrays and Multiplexing
J. Zhou (2013)
Surface profiles of live colorectal cancer cells and tumor infiltrating lymphocytes from surgical samples correspond to prognostic categories.
J. Zhou (2015)
Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles
J. Lötvall (2014)
MEC1 and MEC2: two new cell lines derived from B-chronic lymphocytic leukaemia in prolymphocytoid transformation.
A. Stacchini (1999)
Extracellular vesicles as a platform for ‘liquid biopsy’ in glioblastoma patients
David R. Santiago-Dieppa (2014)
CD20 levels determine the in vitro susceptibility to rituximab and complement of B-cell chronic lymphocytic leukemia: further regulation by CD55 and CD59.
J. Golay (2001)
Differentiating chronic lymphocytic leukemia from monoclonal B-lymphocytosis according to clinical outcome: on behalf of the GIMEMA chronic lymphoproliferative diseases working group
S. Molica (2011)
Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples
L. Belov (2016)
Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane
A. Booth (2006)
Differential fates of biomolecules delivered to target cells via extracellular vesicles
Masamitsu Kanada (2015)
Circulating microvesicles in B-cell chronic lymphocytic leukemia can stimulate marrow stromal cells: implications for disease progression.
A. Ghosh (2010)
Extracellular vesicle transfer of cancer pathogenic components
Y. Fujita (2016)
Surface antigen profiling of colorectal cancer using antibody microarrays with fluorescence multiplexing.
J. Zhou (2010)
Proteomics Analysis of A33 Immunoaffinity-purified Exosomes Released from the Human Colon Tumor Cell Line LIM1215 Reveals a Tissue-specific Protein Signature*
S. Mathivanan (2009)
The applicability of a cluster of differentiation monoclonal antibody microarray to the diagnosis of human disease.
P. Ellmark (2008)
Tumor-derived microvesicles: shedding light on novel microenvironment modulators and prospective cancer biomarkers.
C. D'Souza-Schorey (2012)
CD98 at the crossroads of adaptive immunity and cancer
Joseph M. Cantor (2012)
Cancer statistics, 2014
R. Siegel (2014)
Extracellular vesicle-mediated transfer of donor genomic DNA to recipient cells is a novel mechanism for genetic influence between cells.
J. Cai (2013)
Prognostic impact of epithelial cell adhesion molecule in ovarian cancer patients
M. Lee (2014)
Analysis of Post-Liver Transplant Hepatitis C Virus Recurrence Using Serial Cluster of Differentiation Antibody Microarrays
W. Rahman (2015)
CD8α is expressed by human monocytes and enhances FcγR-dependent responses
Derrick J. Gibbings (2007)
Optimized Normalization for Antibody Microarrays and Application to Serum-Protein Profiling*S
Darren Hamelinck (2005)
Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles
Jinghuan Li (2015)
Conservation of unique cell-surface CD antigen mosaics in HIV-1-infected individuals.
A. Woolfson (2005)
Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages
Jing Qin Wu (2007)
Guidelines for the diagnosis and treatment of chronic lymphocytic leukemia: a report from the International Workshop on Chronic Lymphocytic Leukemia updating the National Cancer Institute-Working Group 1996 guidelines.
M. Hallek (2008)
Staging of colorectal cancer
N. Davis (1984)
Getting to Know Ovarian Cancer Ascites: Opportunities for Targeted Therapy-Based Translational Research
N. Ahmed (2013)
Effect of 3.2% vs 3.8% sodium citrate concentration on routine coagulation testing.
D. Adcock (1997)
Cell-derived Extracellular Vesicles Open New Perspectives for Cancer Research
Irène Tatischeff (2015)
High Levels of Exosomes Expressing CD63 and Caveolin-1 in Plasma of Melanoma Patients
M. Logozzi (2009)
Exosomes serve as tumour markers for personalized diagnostics owing to their important role in cancer metastasis
Taixue An (2015)
Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma
Hina Kalra (2013)
Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
F. Royo (2016)
Human Serum IgM Glycosylation
J. N. Arnold (2005)
Importance of exosome depletion protocols to eliminate functional and RNA-containing extracellular vesicles from fetal bovine serum
G. Shelke (2014)
Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray
L. Belov (2006)
Isotype controls in phenotyping and quantification of microparticles: a major source of error and how to evade it.
A. Trummer (2008)
Ectosomes and exosomes: shedding the confusion between extracellular vesicles.
Emanuele Cocucci (2015)
Higher-Order Oligomerization Targets Plasma Membrane Proteins and HIV Gag to Exosomes
Y. Fang (2007)
Dendritic Cell-Derived Exosomes Express a Streptococcus pneumoniae Capsular Polysaccharide Type 14 Cross-Reactive Antigen That Induces Protective Immunoglobulin Responses against Pneumococcal Infection in Mice
J. Colino (2006)
Longitudinal microarray analysis of cell surface antigens on peripheral blood mononuclear cells from HIV+ individuals on highly active antiretroviral therapy
Jing Qin Wu (2007)
Microparticle‐associated tissue factor activity: a link between cancer and thrombosis?
M. Tesselaar (2007)
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Shivakumar Keerthikumar (2015)
Tumour-derived exosomes and their role in cancer-associated T-cell signalling defects
D. D. Taylor (2005)
This paper is referenced by
Extracellular vesicles in cancer — implications for future improvements in cancer care
R. Xu (2018)
Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence
Maria S. Panagopoulou (2020)
Colorectal Cancer Cell Line SW480 and SW620 Released Extravascular Vesicles: Focus on Hypoxia-induced Surface Proteome Changes
I. Nakurte (2018)
Microvesicles in Cancer: Small Size, Large Potential
Kerstin Menck (2020)