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Profiling Open Chromatin Structure In The Ovarian Somatic Cells Using ATAC-seq.

Kensaku Murano, Yuka W Iwasaki, Haruhiko Siomi
Published 2018 · Medicine, Biology
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The assay for transposase-accessible chromatin using sequencing (ATAC-seq) was recently established as a method to profile open chromatin, which overcomes the sample size limitations of the alternative methods DNase/MNase-seq. To investigate the role of Piwi in heterochromatin formation around transposable element loci, we have used ATAC-seq to examine chromatin accessibility at target transposable elements in a Drosophila cultured cell line, ovarian somatic cells (OSCs). In this chapter, we describe our method to profile open chromatin structure in OSCs using ATAC-seq.

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