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Cell Viability Assays: Introduction.

M. Stoddart
Published 2011 · Chemistry, Medicine

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The measurement of cell viability plays a fundamental role in all forms of cell culture. Sometimes it is the main purpose of the experiment, such as in toxicity assays. Alternatively, cell viability can be used to -correlate cell behaviour to cell number, providing a more accurate picture of, for example, anabolic -activity. There are wide arrays of cell viability methods which range from the most routine trypan blue dye exclusion assay to highly complex analysis of individual cells, such as using RAMAN microscopy. The cost, speed, and complexity of equipment required will all play a role in determining the assay used. This chapter aims to provide an overview of many of the assays available today.
This paper references
10.1002/cyto.990130216
Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry.
I. Schmid (1992)
10.1038/284555a0
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
10.1083/jcb.119.3.493
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
Y. Gavrieli (1992)
10.1089/154065804322966315
Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays.
T. Riss (2004)
10.1002/cyto.a.20535
SYTO probes in the cytometry of tumor cell death
D. Wlodkowic (2008)
10.1016/0022-1759(83)90303-4
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
K. T. Brunner (1968)
10.1016/j.cell.2009.08.021
Caspases and Kinases in a Death Grip
M. Kurokawa (2009)
10.1038/bjc.1972.33
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
10.1046/J.1432-1327.2000.01606.X
Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.
J. O'Brien (2000)
10.1182/blood.V84.5.1415.bloodjournal8451415
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis.
G. Koopman (1994)
Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines.
D. Scudiero (1988)
10.22203/ECM.V012A02
A comparison of non-radioactive methods for assessing viability in ex vivo cultured cancellous bone: technical note.
M. Stoddart (2006)
10.1046/j.1471-4159.1997.69020581.x
Mechanism of Cellular 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) Reduction
Y. Liu (1997)
10.2106/00004623-198567020-00015
The effect of age on bone composition and viability in the femoral head.
S. Y. Wong (1985)
10.3727/095535491820873191
Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture.
A. Cory (1991)
10.3109/00313028209092124
The Determination of Bone Viability: A Histochemical Method for Identification of Lactate Dehydrogenase Activity in Osteocytes in Fresh Calcified and Decalcified Sections of Human Bone
S. Y. Wong (1982)



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10.3384/diss.diva-145300
Organs-on-chips for the pharmaceutical development process : design perspectives and implementations
Jonas Christoffersson (2018)
Assessment of the toxicity of lipid nanocapsules and polymer nanoparticles on a neuronal cell model as a first step towards their development for nose-to-brain drug delivery
Rifka Nurul Utami (2019)
10.1007/s13204-015-0422-x
Characterization and in vitro studies on anticancer, antioxidant activity against colon cancer cell line of gold nanoparticles capped with Cassia tora SM leaf extract
Ezra Elumalai Abel (2015)
10.1007/s11095-019-2748-7
Analysis of In Vitro Cytotoxicity of Carbohydrate-Based Materials Used for Dissolvable Microneedle Arrays
Ezgi P Yalcintas (2020)
10.1016/j.jcyt.2012.11.017
Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering.
Miguel Angel Martin-Piedra (2013)
10.1201/9781315371443-3
Measurement of Toxicants and Toxicity
Alexander Gosslau (2016)
10.3390/foods9050555
Preparation and Evaluation of New Glycopeptides Obtained by Proteolysis from Corn Gluten Meal Followed by Transglutaminase-Induced Glycosylation with Glucosamine
Xiao-lan Liu (2020)
10.1002/cptx.21
Quantification of Lactate Dehydrogenase for Cell Viability Testing Using Cell Lines and Primary Cultured Astrocytes
S. Kaja (2017)
Combination of new multifunctional molecules for erythematotelangiectatic rosacea disorder
Orléans Cedex CBM-Cnrs (2015)
10.3389/fphar.2019.00884
Considerations for an In Vitro, Cell-Based Testing Platform for Detection of Adverse Drug-Induced Inotropic Effects in Early Drug Development. Part 1: General Considerations for Development of Novel Testing Platforms
B. Guth (2019)
10.1016/j.jtbi.2020.110222
Systems biology of ferroptosis: A modeling approach.
Anna Konstorum (2020)
10.1007/s12223-015-0399-6
In vitro effects on biofilm viability and antibacterial and antiadherent activities of silymarin
E. Evren (2015)
10.1080/19440049.2013.790086
Providencia alcalifaciens causes barrier dysfunction and apoptosis in tissue cell culture: potent role of lipopolysaccharides on diarrheagenicity
Hiroshi Asakura (2013)
10.1007/s10103-020-03016-z
Photobiomodulation alters the viability of HUVECs cells
Stella Maris Lins Terena (2020)
10.1016/j.taap.2020.115071
Eprinomectin, a novel avermectin macrocylic lactone is cytotoxic to PC3 metastatic prostate cancer cells via inducing apoptosis.
Angela Lincy Prem Antony Samy (2020)
10.1080/15376516.2017.1313345
In vitro study of putative genomic biomarkers of nephrotoxicity through differential gene expression using gentamicin
Sarah Cristina Teixeira Silva (2017)
Mitochondrial localisation and cellular uptake in vitro using novel ‘mitochondriotropic’ liposomes.
Nicolisha. Narainpersad (2016)
10.3109/10520295.2014.916415
Quirks of dye nomenclature. 3. Trypan blue
C J Cooksey (2014)
10.3390/polym12051205
The Effect of Chitosan on the Chemical Structure, Morphology, and Selected Properties of Polyurethane/Chitosan Composites
Agnieszka Piotrowska-Kirschling (2020)
10.3390/s120912347
Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays
S. Rampersad (2012)
10.3109/10520295.2014.916415
Quirks of dye nomenclature. 3. Trypan blue.
Cooksey Cj (2014)
10.1007/978-1-4939-8994-2_27
The Use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity.
Paul c. Guest (2019)
Thyroid Hormone Mediation of T cell Proliferation and Survival; Implications for Hypothyroidism and Hyperthyroidism.
Tanya Babiuk-Henry (2016)
Cytotoxic Effects of Aqueous Extract of Portulaca oleracea on Oral Cancer Cell Line
Zahra Azarifar (2018)
10.1002/jbio.201500108
Automated quantitative cytological analysis using portable microfluidic microscopy.
Veerendra Kalyan Jagannadh (2016)
10.1089/TEN.TEC.2017.0371
Noninvasive Real-Time Assessment of Cell Viability in a Three-Dimensional Tissue.
MahfouziSeyed Hossein (2018)
10.1016/j.freeradbiomed.2019.08.006
Opposing action of NCoR1 and PGC-1α in mitochondrial redox homeostasis.
Tanes I. Lima (2019)
10.1016/j.bbagen.2018.04.016
STAT-3 regulation of CXCR4 is necessary for the prenylflavonoid Icaritin to enhance mesenchymal stem cell proliferation, migration and osteogenic differentiation.
R. Lim (2018)
10.1007/s10637-017-0483-7
Urolithins impair cell proliferation, arrest the cell cycle and induce apoptosis in UMUC3 bladder cancer cells
J. Liberal (2017)
10.1007/978-3-030-39923-8_13
Toxicity Assessment of Nanomaterials
M. Tasso (2020)
10.4103/0973-1296.139804
The Pavonia xanthogloea (Ekman, Malvaceae): Phenolic compounds quantification, anti-oxidant and cytotoxic effect on human lymphocytes cells
C. P. Mostardeiro (2014)
10.1364/AO.52.00A300
Live tissue viability and chemosensitivity assays using digital holographic motility contrast imaging.
R. An (2013)
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