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Recombinant Protein Production In Yeasts.

D. Mattanovich, P. Branduardi, Laura Dato, Brigitte Gasser, M. Sauer, D. Porro
Published 2012 · Biology, Medicine

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Recombinant protein production is a multibillion-dollar market. The development of a new product begins with the choice of a production host. While one single perfect host for every protein does not exist, several expression systems ranging from bacterial hosts to mammalian cells have been established. Among them, yeast cell factories combine the advantages of being single cells, such as fast growth and easy genetic manipulation, as well as eukaryotic features including a secretory pathway leading to correct protein processing and post-translational modifications. In this respect, especially the engineering of yeast glycosylation to produce glycoproteins of human-like glycan structures is of great interest. Additionally, different attempts of cellular engineering as well as the design of different production processes that are leading to improved productivities are presented. With the advent of cheaper next-generation sequencing techniques, systems biotechnology approaches focusing on genome scale analyses will advance and accelerate yeast cell factories and thus recombinant protein production processes in the near future. In this review we summarize advantages and limitations of the main and most promising yeast hosts, including Saccharomyces cerevisiae, Pichia pastoris, and Hansenula polymorpha as those presently used in large scale production of heterologous proteins.
This paper references
10.1016/j.jbiotec.2008.12.015
Production of monoclonal antibodies by glycoengineered Pichia pastoris.
Thomas I. Potgieter (2009)
10.1007/BF00326302
Multiple-copy integration in the yeast Yarrowia lipolytica
M. L. Dall (2004)
10.1002/(SICI)1097-0061(1998100)14:14<1267::AID-YEA327>3.0.CO;2-2
Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica.
S. Mueller (1998)
10.1002/BIT.20871
Targeting early apoptotic genes in batch and fed‐batch CHO cell cultures
D. C. F. Wong (2006)
10.1016/S0378-1119(96)00675-0
Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter.
H. Waterham (1997)
Bio drugs to dominate top ten list by 2014. In-Pharma Technologist
G. Macdonald (2009)
Transformation of Yeast by the LiAc/ss Carrier DNA/ PEG
R. D. Gietz (2002)
10.1021/bp025516w
Design of Methanol Feed Control in Pichia pastoris Fermentations Based upon a Growth Model
W. Zhang (2002)
10.1016/J.FGB.2007.09.012
Isolation of four pepsin-like protease genes from Aspergillus niger and analysis of the effect of disruptions on heterologous laccase expression.
Yongchao Wang (2008)
10.1002/YEA.1545
A systems biology perspective of wine fermentations.
F. Pizarro (2007)
10.1002/btpr.524
Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis
Martin Dragosits (2011)
10.1248/BPB.32.786
Glycan engineering and production of 'humanized' glycoprotein in yeast cells.
Y. Chiba (2009)
10.1186/1475-2859-8-29
Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris
D. Mattanovich (2009)
10.1007/s00253-010-2447-0
Engineering of protein secretion in yeast: strategies and impact on protein production
A. Idiris (2010)
in the yeast Pichia pastoris
D. Resina (2004)
10.1111/J.1567-1364.2006.00116.X
Production of Man5GlcNAc2-type sugar chain by the methylotrophic yeast Ogataea minuta.
Kousuke Kuroda (2006)
10.1016/0168-1605(96)01124-5
Evaluation of media for enumerating heat-stressed, benzoate-resistant Zygosaccharomyces bailii.
A. K. Makdesi (1996)
10.1007/BF00167913
Heterologous gene expression in continuous cultures of budding yeast
D. Porro (1991)
and C Leão environments . on the resistance of the yeast to acidic bailii : effects of ethanol and their implications Transport of acetic acid in Zygosaccharomyces
M. J. Sousa (1996)
10.1186/1475-2859-2-4
Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
Jari Rautio (2003)
10.1186/1475-2859-5-37
Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris
Michael Maurer (2006)
10.1038/nbt1290
Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis
T. Jeffries (2007)
10.1038/nbt0591-455
High-Level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integrations of the Gene
J. Clare (1991)
10.1038/nprot.2008.213
Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology
P. P. Jacobs (2008)
10.1016/0167-7799(93)90007-V
The production of heterologous plasma proteins.
A. R. Goodey (1993)
10.1002/bit.22756
Antibody expression kinetics in glycoengineered Pichia pastoris
Thomas I. Potgieter (2010)
10.1016/j.jbiotec.2009.06.016
Disruption of the OCH1 and MNN1 genes decrease N-glycosylation on glycoprotein expressed in Kluyveromyces lactis.
B. Liu (2009)
Vector-mediated overexpression of catalase A in the yeast Saccharomyces cerevisiae induces inclusion body formation.
M. Binder (1991)
10.1002/yea.320100703
Genetic interrelationship among species of the genus Zygosaccharomyces as revealed by small-subunit rRNA gene sequences.
S. James (1994)
10.1093/glycob/cwn008
Engineering of a mammalian O-glycosylation pathway in the yeast Saccharomyces cerevisiae: production of O-fucosylated epidermal growth factor domains.
Yuko Chigira (2008)
10.1007/s002530000464
Heterologous protein production in methylotrophic yeasts
G. Gellissen (2000)
10.1002/1097-0061(20000930)16:13<1191::AID-YEA589>3.0.CO;2-2
Constitutive expression of recombinant proteins in the methylotrophic yeast Hansenula polymorpha using the PMA1 promoter.
H. Cox (2000)
10.1093/jb/mvp166
Signalling pathways in the unfolded protein response: development from yeast to mammals.
K. Mori (2009)
10.1038/nbt1020
The evolution of molecular biology into systems biology
H. Westerhoff (2004)
10.1007/s002530051083
Biosynthetic production of type II fish antifreeze protein: fermentation by Pichia pastoris
M. Loewen (1997)
10.1007/s10529-010-0289-6
N-Linked glycoengineering for human therapeutic proteins in bacteria
Jagroop Pandhal (2010)
10.1186/1475-2859-8-22
Process development in Hansenula polymorpha and Arxula adeninivorans, a re-assessment
C. Stöckmann (2009)
10.1002/yea.320080602
Foreign gene expression in yeast: a review.
M. Romanos (1992)
10.1038/nbt.1544
Genome sequence of the recombinant protein production host Pichia pastoris
K. D. Schutter (2009)
10.1007/BF00422632
Methylotrophic yeasts — 1986
G. H. Wegner (2004)
10.1126/SCIENCE.1088166
Production of Complex Human Glycoproteins in Yeast
S. R. Hamilton (2003)
10.1042/BA20090030
Methanol‐inducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidinii
H. Yurimoto (2009)
10.1128/AEM.01196-07
Transcriptomics-Based Identification of Novel Factors Enhancing Heterologous Protein Secretion in Yeasts
Brigitte Gasser (2007)
10.1016/S1567-1356(03)00125-9
The Hansenula polymorpha (strain CBS4732) genome sequencing and analysis.
Massoud Ramezani-Rad (2003)
10.1002/biot.200700252
Glycoengineering of the methylotrophic yeast Hansenula polymorpha for the production of glycoproteins with trimannosyl core N-glycan by blocking core oligosaccharide assembly.
Doo-Byoung Oh (2008)
10.14429/DSJ.59.1501
High Yield Production of Heterologous Proteins with Escherichia coli
N. Tripathi (2009)
10.1111/J.1567-1364.2006.00049.X
Heterologous protein production in the yeast Kluyveromyces lactis.
A. V. van Ooyen (2006)
10.1111/j.1567-1364.2010.00668.x
Advances in molecular tools for the use of Zygosaccharomyces bailii as host for biotechnological productions and construction of the first auxotrophic mutant.
Laura Dato (2010)
10.1042/bst0161081a
Hansenula polymorpha as a novel yeast system for the expression of heterologous genes.
P. Sudbery (1988)
10.1016/S1567-1356(03)00200-9
The yeast Zygosaccharomyces bailii: a new host for heterologous protein production, secretion and for metabolic engineering applications.
P. Branduardi (2004)
10.1128/AEM.66.12.5477-5479.2000
Improved Secretion of Native Human Insulin-Like Growth Factor 1 from gas1 Mutant Saccharomyces cerevisiae Cells
M. Vai (2000)
10.1002/(SICI)1097-0061(19980115)14:1<11::AID-YEA196>3.0.CO;2-S
Development of the methylotrophic yeast Pichia methanolica for the expression of the 65 kilodalton isoform of human glutamate decarboxylase.
C. Raymond (1998)
10.1007/s00284-006-0585-5
Influence of N-Glycosylation on Saccharomyces cerevisiae Morphology: A Golgi Glycosylation Mutant Shows Cell Division Defects
Jungang Zhou (2006)
10.1093/jb/mvp196
Stress-sensing mechanisms in the unfolded protein response: similarities and differences between yeast and mammals.
K. Kohno (2010)
10.1016/0168-1656(93)90114-3
High level expression in Saccharomyces cerevisiae of an artificial gene encoding a repeated tripeptide aspartyl-phenylyalanyl-lysine.
S. Y. Choi (1993)
10.1186/1475-2859-2-6
Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
M. Jahic (2003)
The cellular response to protein misfolding in the endoplasmic reticulum.
A. Welihinda (1999)
10.1186/1475-2859-3-17
Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation
M. Sauer (2004)
10.1007/3-540-36466-8_3
Bioprocessing of therapeutic proteins from the inclusion bodies of Escherichia coli.
A. Panda (2003)
10.1271/bbb.70582
Monitoring Global Gene Expression of Proteases and Improvement of Human Lysozyme Production in the nptB Gene Disruptant of Aspergillus oryzae
S. Kimura (2008)
10.1016/S0958-1669(97)80028-6
Production of recombinant proteins by methylotrophic yeasts.
C. Hollenberg (1997)
10.1002/1097-0290(20010105)72:1<1::AID-BIT1>3.0.CO;2-T
A rational approach to improving productivity in recombinant Pichia pastoris fermentation.
M. Danjou (2001)
10.1385/MB:16:1:23
Recombinant protein expression in Pichia pastoris
J. Cregg (2000)
10.1186/1475-2859-9-4
Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
S. N. Rocha (2010)
10.1002/j.1460-2075.1992.tb05316.x
OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine‐linked oligosaccharides.
K. Nakayama (1992)
10.1186/1471-2164-11-S4-S5
dbDEMC: a database of differentially expressed miRNAs in human cancers
Z. Yang (2010)
10.1002/BIT.10556
Metabolic analysis of the synthesis of high levels of intracellular human SOD in Saccharomyces cerevisiae rhSOD 2060 411 SGA122.
R. Gonzalez (2003)
10.1016/J.JBIOTEC.2007.01.031
Systems biology for industrial strains and fermentation processes--example: amino acids.
R. Takors (2007)
10.1016/J.JBIOTEC.2003.10.027
Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review.
C. Madzak (2004)
10.1021/bp060171t
Process Technology for Production and Recovery of Heterologous Proteins with Pichia pastoris
M. Jahic (2006)
Structure of the N-linked oligosaccharides that show the complete loss of alpha-1,6-polymannose outer chain from och1, och1 mnn1, and och1 mnn1 alg3 mutants of Saccharomyces cerevisiae.
Y. Nakanishi-Shindo (1993)
10.1016/S0076-6879(02)50957-5
Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method.
R. D. Gietz (2002)
10.1128/AEM.70.5.2639-2646.2004
In Vivo Synthesis of Mammalian-Like, Hybrid-Type N-Glycans in Pichia pastoris
W. Vervecken (2004)
10.1002/yea.320101006
Physiological approach to heterologous human serum albumin production by Kluyveromyces lactis in chemostat culture.
K. Blondeau (1994)
10.1016/0958-1669(95)80087-5
Advances in the use of Pichia pastoris for high-level gene expression
M. Romanos (1995)
10.1016/0003-2697(87)90240-5
Transformation of yeast spheroplasts without cell fusion.
P. Burgers (1987)
10.1007/s00253-009-2167-5
Xplor® 2—an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans
E. Böer (2009)
10.1016/J.MIMET.2005.08.010
Rapid and multiplexed transcript analysis of microbial cultures using capillary electophoresis-detectable oligonucleotide probe pools.
J. Rautio (2006)
The effects of drugs inhibiting protein secretion in the fi lamentous fungus Trichoderma reesei . Evidence for down - regulation of genes that encode secreted proteins in the stressed cells
C. Swaim (2008)
10.1016/S1389-1723(00)80082-1
High level secretion of recombinant human serum albumin by fed-batch fermentation of the methylotrophic yeast, Pichia pastoris, based on optimal methanol feeding strategy.
K. Kobayashi (2000)
10.1007/s00253-007-1209-0
Yeast expression platforms
E. Böer (2007)
10.1016/S1567-1356(03)00175-2
Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora.
C. Kurtzman (2003)
10.1271/bbb1961.54.2521
Secretion of Aspergillus oryzae alkaline protease in an osmophilic yeast, Zygosaccharomyces rouxii.
Y. Ogawa (1990)
10.1186/1471-2164-11-1
Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis
Tim van Zutphen (2009)
10.1201/b18525-14
Metabolic engineering
L. Wackett (2000)
10.2436/IM.V1I2.58
Non-conventional yeasts as hosts for heterologous protein production.
A. Domínguez (1998)
10.1099/MIC.0.29263-0
Metabolic flux profiling of Pichia pastoris grown on glycerol/methanol mixtures in chemostat cultures at low and high dilution rates.
A. Solà (2007)
10.1038/nbt1191-1067
Yeast Systems for the Commercial Production of Heterologous Proteins
R. Buckholz (1991)
10.1023/A:1008081314112
Purification of Recombinant Human Serum Albumin Efficient purification using STREAMLINE
A. Sumi (1999)
10.1016/S0958-1669(99)00004-X
Applications of yeast in biotechnology: protein production and genetic analysis.
G. L. Cereghino (1999)
10.1002/BIT.20851
Engineering of Pichia pastoris for improved production of antibody fragments
Brigitte Gasser (2006)
10.1016/S1567-1356(03)00148-X
Integration of heterologous genes in several yeast species using vectors containing a Hansenula polymorpha-derived rDNA-targeting element.
J. Klabunde (2003)
10.1002/YEA.1208
Heterologous protein production using the Pichia pastoris expression system.
Sue Macauley-Patrick (2005)
10.1016/S0958-1669(96)80055-3
The expression of recombinant proteins in yeasts.
P. Sudbery (1996)
10.1186/1475-2859-8-17
Microbial factories for recombinant pharmaceuticals
N. Ferrer-Miralles (2009)
Trans - formation of Yeast by the LiAc / ss Carrier DNA / PEG . Method
M. Sanchez (1993)
10.1155/2008/735101
Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production
Mee-Jung Han (2008)
10.1021/pr8007623
The effect of temperature on the proteome of recombinant Pichia pastoris.
Martin Dragosits (2009)
10.1016/J.FEMSYR.2005.06.004
New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica - a comparison.
G. Gellissen (2005)
10.1023/A:1005340806821
Modification of the protein glycosylation pathway in the methylotrophic yeast Pichia pastoris
W. Martinet (2004)
10.1111/J.1567-1364.2001.TB00024.X
Zygosaccharomyces kombuchaensis, a new ascosporogenous yeast from 'Kombucha tea'.
C. Kurtzman (2001)
10.1002/bit.22836
Quantitative physiology of Pichia pastoris during glucose‐limited high‐cell density fed‐batch cultivation for recombinant protein production
J. Heyland (2010)
10.1074/jbc.M302372200
The Effects of Drugs Inhibiting Protein Secretion in the Filamentous Fungus Trichoderma reesei
T. Pakula (2003)
10.1016/j.mib.2010.04.004
Systems biology of energy homeostasis in yeast.
J. Zhang (2010)
10.1111/j.1567-1364.2009.00561.x
Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus.
H. Marx (2009)
10.1186/1475-2859-7-11
Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview
Brigitte Gasser (2008)
10.1016/J.JBIOTEC.2003.10.029
Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter.
David Resina (2004)
10.1016/0167-4781(88)90135-2
The expression of functional ricin B-chain in Saccharomyces cerevisiae.
P. T. Richardson (1988)
10.1002/pmic.200700764
Physical and computational analysis of the yeast Kluyveromyces lactis secreted proteome.
Catherine L Swaim (2008)
10.1186/1471-2164-8-179
Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions
Brigitte Gasser (2007)
10.1016/S0304-4165(98)00126-3
Overview of N- and O-linked oligosaccharide structures found in various yeast species.
T. Gemmill (1999)
10.1002/bit.22868
Integrative analysis using proteome and transcriptome data from yeast to unravel regulatory patterns at post‐transcriptional level
R. Olivares-Hernández (2010)
10.1016/J.JBIOTEC.2006.12.024
Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures.
C. Jungo (2007)
10.1007/BF00351696
Multiple-copy integration of the α-galactosidase gene from Cyamopsis tetragonoloba into the ribosomal DNA of Kluyveromyces lactis
R. Bergkamp (2004)
10.1186/1475-2859-9-7
Cloning of the Zygosaccharomyces bailii GAS1 homologue and effect of cell wall engineering on protein secretory phenotype
S. Passolunghi (2010)
10.1007/978-94-009-3539-6_35
The Methylotrophic Yeast Pichia Pastoris as a Host for Heterologous Protein Production
G. Thill (1987)
10.1128/aem.59.7.2087-2092.1993
Transformation of Kluyveromyces lactis by Electroporation.
M. Sánchez (1993)
Protocol for effi cient secretion of HSA developed from Pichia pastoris
K. A. Barr (1992)
Purifi cation of recombinant human serum albumin . Effi cient purifi cation using STREAMLINE
H. Westerhoff (2004)
10.1021/bp060012+
Evaluation of Mut+ and MutS Pichia pastoris Phenotypes for High Level Extracellular scFv Expression under Feedback Control of the Methanol Concentration
Itzcoatl A. Pla (2006)
10.1271/bbb.80825
Molecular Basis of Methanol-Inducible Gene Expression and Its Application in the Methylotrophic Yeast Candida boidinii
H. Yurimoto (2009)
10.1016/S0955-0674(00)00219-2
Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response in yeast and mammals.
C. Patil (2001)
10.1128/AEM.69.4.2065-2072.2003
Effects of Inactivation and Constitutive Expression of the Unfolded- Protein Response Pathway on Protein Production in the Yeast Saccharomyces cerevisiae
Mari Valkonen (2003)
10.1016/j.nbt.2009.01.008
Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter.
David Resina (2009)
10.1007/BFb0009080
Transfer and expression of heterologous genes in yeasts other than Saccharomyces cerevisiae.
J. Reiser (1990)
10.1111/j.1567-1364.2009.00507.x
Yeast systems biotechnology for the production of heterologous proteins.
Alexandra B. Graf (2009)
10.1038/293717a0
Expression of a human gene for interferon in yeast
R. Hitzeman (1981)
10.1016/j.copbio.2008.05.003
Importance of systems biology in engineering microbes for biofuel production.
A. Mukhopadhyay (2008)
10.1016/S0141-0229(00)00173-3
The methylotrophic yeast Hansenula polymorpha: a versatile cell factory.
van Dijk R (2000)
10.1038/nrd3040
Market watch: Sales of biologics to show robust growth through to 2013
M. Goodman (2009)
The effects of drugs inhibiting protein secretion in the fi lamentous fungus Trichoderma reesei . Evidence for down - regulation of genes that encode secreted proteins in the stressed cells
C. Swaim (2008)
10.1021/bp025518g
Decreased Protein Expression and Intermittent Recoveries in BiP Levels Result from Cellular Stress during Heterologous Protein Expression in Saccharomyces cerevisiae
Kenneth J. Kauffman (2002)
10.1126/science.274.5287.546
Life with 6000 Genes
A. Goffeau (1996)
10.1007/s10529-009-0077-3
Extracellular recombinant protein production from Escherichia coli
Ye Ni (2009)
10.1007/s00253-010-2721-1
Engineering of glycosylation in yeast and other fungi: current state and perspectives
Karen De Pourcq (2010)
10.1007/s00253-008-1458-6
The yeast Kluyveromyces marxianus and its biotechnological potential
G. Fonseca (2008)
10.1128/aem.62.9.3152-3157.1996
Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and their implications on the resistance of the yeast to acidic environments.
M. J. Sousa (1996)
10.1111/J.1574-6968.2006.00427.X
Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris.
H. Marx (2006)
10.1055/S-2006-957461
Recombinant expression of alliin lyase from garlic (Allium sativum) in bacteria and yeasts.
R. Weik (1998)
10.1016/B978-012666260-3/50002-9
CHAPTER 1 – The Essence of Metabolic Engineering
G. Stephanopoulos (1998)
10.1016/S1097-2765(00)80251-8
Degradation of proteins from the ER of S. cerevisiae requires an intact unfolded protein response pathway.
R. Casagrande (2000)
Infl uence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis, Biotechnology Progress
M. Dragosits (2010)
10.1038/nrmicro1087
The humanization of N-glycosylation pathways in yeast
S. Wildt (2005)
10.1093/nar/gkn369
Promoter library designed for fine-tuned gene expression in Pichia pastoris
F. Hartner (2008)
10.1271/bbb.70080
Protein O-Glycosylation in Fungi: Diverse Structures and Multiple Functions
M. Goto (2007)
10.1016/S0167-7012(00)00171-8
Relating growth dynamics and glucoamylase excretion of individual Saccharomyces cerevisiae cells.
D. Porro (2000)
Induction of oxidofermentative ethanol formation in recombinant cells of Saccharomyces cerevisiae yeast.
R. Weik (1999)
10.1074/jbc.271.7.3398
Golgi Localization and in Vivo Activity of a Mammalian Glycosyltransferase (Human 1,4-Galactosyltransferase) in Yeast (*)
T. Schwientek (1996)
10.1042/BA20090048
Production of heterologous proteins using the fission‐yeast (Schizosaccharomyces pombe) expression system
K. Takegawa (2009)
10.1007/s002530100790
Differential expression of the Trichoderma reesei β-xylanase II (xyn2) gene in the xylose-fermenting yeast Pichia stipitis
R. D. Haan (2001)
10.1016/S0378-1119(97)00020-6
Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis -- a review.
G. Gellissen (1997)
10.1186/1471-2164-11-207
The response to unfolded protein is involved in osmotolerance of Pichia pastoris
Martin Dragosits (2009)
Hansenula polymorpha using the PMA 1 promoter
D. B. Oh (2008)
10.1007/s002530000444
Genetic transformation and biotechnological application of the yeast Arxula adeninivorans
T. Wartmann (2000)
10.1111/J.1574-6976.2000.TB00532.X
Heterologous protein expression in the methylotrophic yeast Pichia pastoris.
J. L. Cereghino (2000)
10.1073/pnas.0931263100
Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris
Byung-Kwon Choi (2003)
10.1111/J.1432-1033.2004.04176.X
Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris.
A. Solà (2004)
10.1111/j.1470-8744.1999.tb00776.x
Expression system for foreign genes using the fission yeast Schizosaccharomyces pombe
Y. Giga-Hama (1999)
10.1002/BIT.10297
Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris.
Feng F Hong (2002)
10.1016/S0378-1119(98)00315-1
A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris.
S. Shen (1998)
10.1006/PREP.1997.0765
High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts.
M. Hasslacher (1997)
10.4014/JMB.0901.018
Optimization of the functional expression of Coprinus cinereus peroxidase in Pichia pastoris by varying the host and promoter.
S. Kim (2009)
10.1002/BIT.10904
Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris
Hubertus Hohenblum (2004)
10.1016/J.JBIOTEC.2004.04.035
Stress in recombinant protein producing yeasts.
D. Mattanovich (2004)
10.1016/S0168-1656(03)00049-X
Assessing viability and cell-associated product of recombinant protein producing Pichia pastoris with flow cytometry.
Hubertus Hohenblum (2003)
10.1007/BF00191182
Expression of Saccharomyces adenylate kinase gene in Candida boidinii under the regulation of its alcohol oxidase promoter
Y. Sakai (2004)
Transcriptomicsbased identifi cation of novel factors enhancing heterologous protein secretion
B. Gasser (2007)
10.1038/nature02579
Genome evolution in yeasts
B. Dujon (2004)
10.1016/J.COPBIO.2007.09.001
Glycosylation engineering in yeast: the advent of fully humanized yeast.
S. R. Hamilton (2007)
10.1128/AEM.02058-06
Engineering of the Yeast Yarrowia lipolytica for the Production of Glycoproteins Lacking the Outer-Chain Mannose Residues of N-Glycans
Yunkyoung Song (2007)
10.1016/J.JBIOTEC.2007.08.004
Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium.
Tobias Fürch (2007)
10.1021/bp000164j
Human Chymotrypsinogen B Production with Pichiapastoris by Integrated Development of Fermentation and Downstream Processing. Part 1. Fermentation
S. Curvers (2001)
10.1093/GLYCOB/CWH104
Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: production of complex humanized glycoproteins with terminal galactose.
P. Bobrowicz (2004)



This paper is referenced by
10.1007/s00253-010-3019-z
Production of recombinant proteins and metabolites in yeasts
D. Porro (2010)
Procédé de production d'une protéine par précipitation
野中 孝裕 (2014)
Structural Investigations of the Fungal Lytic Polysaccharide Monooxygenase Reaction Mechanism using X-ray and Neutron Protein Crystallography.
William B O'Dell (2017)
10.1007/s12033-012-9531-x
Generation and Comparative Characterization of Glycosylated and Aglycosylated Human IgG1 Antibodies
Dmitrij Hristodorov (2012)
10.1007/978-1-62703-691-7_11
Cell-free protein synthesis for functional and structural studies.
S. Makino (2014)
10.1007/s10858-018-0192-3
Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system
R. Suzuki (2018)
Improving the protein secretion capacity of Saccharomyces cerevisiae with strain engineering
Heinrich Kroukamp (2015)
Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications
Mikael Åstrand (2016)
HIGH LEVEL EXPRESSION OF RECOMBINANT ERYTHROPOIETIN IN HANSENULA POLYMORPHA
Sabah Mohamed Khaled Mahmoud (2014)
10.4995/THESIS/10251/16800
Process development for the obtention and use of recombinant glycosidases: expression, modelling and immobilisation
M. Serra (2012)
10.1007/s00253-012-3933-3
Intracellular interactome of secreted antibody Fab fragment in Pichia pastoris reveals its routes of secretion and degradation
Martin Pfeffer (2012)
10.1039/C4MD00537F
Matrix-immobilized yeast for large-scale production of recombinant human lactoferrin†
Chun Loong Ho (2015)
10.1590/1678-4324-2016150131
Transgenic Expression and Identification of Recombinant Human Proinsulin in Peanut
Zheng Ling (2016)
10.1080/07388551.2019.1566206
Potential application of Leishmania tarentolae as an alternative platform for antibody expression
Jing Yi Lai (2019)
10.1007/7651_2019_249
BacMam System for Rapid Recombinant Protein Expression in Mammalian Cells.
Deepak B Thimiri Govinda Raj (2019)
10.18154/RWTH-2018-225075
What happens in yeast during the Crabtree effect? : an investigation of S. cerevisiae’s volatile space
Christoph Halbfeld (2018)
10.1093/femsyr/fov107
Sucrose and Saccharomyces cerevisiae: a relationship most sweet.
W. Marques (2016)
10.1186/PREACCEPT-4194662891296683
Metabolic engineering of
Zhenquan Lin (2014)
10.1007/s12033-019-00222-1
Edible Vaccines: Promises and Challenges
Vrinda M Kurup (2019)
10.1007/978-3-319-66084-4
A Systems Biology Approach to Advancing Adverse Outcome Pathways for Risk Assessment
N. Garcia-Reyero (2018)
Ciências Biosynthesis and isolation of STEAP 1 protein from Escherichia coli cells lysates
D. Interior (2016)
Production and characterization of human carbonic anhydrase IX in insect cells
Mikaela Lindfors-Huttunen (2014)
10.1007/978-1-4939-0563-8_4
Molecular tools and protocols for engineering the acid-tolerant yeast Zygosaccharomyces bailii as a potential cell factory.
Paola Branduardi (2014)
10.1016/j.ymben.2012.01.002
Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae.
Jin Hou (2012)
10.1002/9783527683321.CH02
Yeast Suspension Culture
Diethard Mattanovich (2014)
Improved production and purification of recombinant proteins from mammalian expression systems
Aw Kinna (2017)
10.1007/10_2018_69
Engineering of Yeast Glycoprotein Expression.
Charlot De Wachter (2018)
10.1002/bab.1186
Two‐step transpeptidation of the insulin precursor expressed in Pichia pastoris to insulin ester via trypsin‐catalyzed cleavage and coupling
H. Liu (2014)
Structural and functional analysis of CD81-Claudin-1, a hepatitis C virus receptor complex
Michelle Clare (2015)
10.11189/BITA.41.97
Methylotrophic recombinant Pichla pastoris for phytase production on D-mannitol/methanol optimization
Ndayambaje Jean Bernard (2018)
10.14279/depositonce-6592
Platform technologies for automated bioprocess development
Florian Glauche (2018)
10.1016/j.biotechadv.2015.05.008
Cultivation strategies to enhance productivity of Pichia pastoris: A review.
V. Looser (2015)
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